Gene Details:

Functional Descriptions:

  • OsWRKY21 and OsWRKY108 interact within the nucleus and both bind to the W-box in the OsPHT1;1 promoter.
  • OsWRKY21 and OsWRKY108 function redundantly to promote phosphate accumulation through maintaining the constitutive expression of OsPHT1;1 under phosphate-replete conditions.
  • Overexpression of OsWRKY21 or OsWRKY108 led to increased Pi accumulation, resulting from elevated expression of OsPHT1;1.
  • By contrast, oswrky21 OsWRKY108 double mutants showed decreased Pi accumulation and OsPHT1;1 expression in a Pi-dependent manner.
  • OsWRKY108 is an integrative regulator of phosphorus homeostasis and leaf inclination in rice.
  • Here, we report that OsWRKY108 acts as a positive regulator of leaf inclination.
  • OsWRKY108 overexpressors showed increased leaf inclination and OsWRKY108-SRDX plants showed an erect-leaf phenotype, irrespective of the Pi regimes.
  • Nevertheless, the response of leaf inclination to Pi starvation was largely impaired upon OsWRKY108 overexpression.
  • Moreover, in both OsWRKY108-SRDX plants and OsWRKY108 overexpressors, the 'percentage of leaf angle alteration relative to wild-type' under Pi-starvation condition was more significant than that under Pi-replete condition.
  • These results suggest that the regulation of OsWRKY108 on leaf inclination is in part dependent on Pi availability.
  • Altogether, our findings demonstrate that OsWRKY108 is an integrative regulator of P homeostasis and leaf inclination, serving as a link between plant nutrient signaling and developmental cues.
  • Overexpression of either OsWRKY108 or OsWRKY21 led to up-regulation of Pi transporter genes and thus enhanced Pi accumulation.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os01g60600.1
    TCTACTTCTGCCGCGCCACCAACCATCCATCGCTGACGCTTCCAACCCAACTGAATTCAAACGAAGCGAACAACACGAACCATGCAGGCGCAATCCCGCCTCGCTGCTGCCGCGAGCGGCGGCAGCGGCAGTGGAATTAGCGGGAGCGGTGGCATTAGCAGGTTGGGTGGTGGAGCGGGGGAGGAGCACGAGGCGGTGGTGAGGGAGCTGACGCGCGGGCACGAGCTGACGGCGCGGCTGCGGGCGGAGGCGCTGCGGGCGCTGCGCGGGCAGGGGCAGGCCGAGGCCACCGCTACATTCATCCTCGGGGAGGTCTCCCGCGCCTTCACCGTCTGCCTCTCCATCATGGCCAGCGCCTCCCCGTCCGCCTCGCCGCCACAACCGGACGAGACGCCGCCGGCCGACTCAGCCGTCTCGCCACCACCACCGCGCGCGGCGAGAGAGGACAACGTCCCGCGAAAGAGATTATTGACAGCCTCTCCTTACGACGATGGGTACCAGTGGAGGAAGTACGGTCAAAAGAAGATCAACAACACCAACTTCCCAAGGAGCTACTACAGGTGCAGTTACCACCGGGAGCGCAGATGCCCCGCGCAGAAGCACGTGCAGCAGCGCGACGGCGACGACGTCCCGGCGCTGCACGTCGTCGTCTACACCCACGAGCACACGTGCCTGCAGGGGGCGCCCGCCGAGCTTCCCGACGCGGCGACGAACGGTGGCGCCGCCGCCGCCGCGTCGCCGGACTACTTCCCCGCCGGCGGCGAGACGCCGTCGTCGCTGCGGCGGCTCCGGGGCGTCGGCGGCGGCGGCCTGCAGCCTCAGTTCGTGGACCACCGCGCCGCCATGGAGGAGCGCGAGCGCCAGGTGCTCGTCTCGTCCCTCGCCCGCGTGCTGCAGGGGCGGCAGTGCTACGACGACGACGACGACGACGACACCGACGTCGCCTCCCTCGGCGCCGTGCACGCGCGCGCGCCTGCGGCGGCGGCGCCGGTCGCCGCGTCGTCGTCGTCGTCCGGGCCCGTCGACGCTGCCGGCGAGGAGCTGGACGTCATGGACTACGACATGACGGACGCGCTGTTCTGGGGGCCTTTCGGCACGGACAGCAACTCGTACGACGGAAACCTGACGTCGACACGCTGTTTTGATCTAATTAATTAATCAGGCGCGCATCGATCCTACATGTGGTTCGGTCGATACATATGACGAGTCGCGGTGCGCATGTCCACGGCTATCGGTAGGCCGTTGGATTCGGATATACTACTACTACTACTACTACTACATTTTGCATGAATCGTATACTACGTACAAGCGTACTACTACTAATTTGCATTACGTATGTACCTTGTGTGTTGTAAAATTAAATCAACCCTGTGTTGTGCAACTATTATGATTGCACTAGGTTATATGGGTTGCGTAGTTGATTAAGTTTGGTCCGGTCGGTTGGAGTGGTTTTTGTATGCACACGTACCAATATGCTGTACGTCTCACTGTCTCGGTGATTCTCACACCAAATAGGCAAAGTGCAAAATTAGCAAAGGCTGTGTTTAGATTTAAAGTTTAGATCCAAACTTTAGTCCTTTTCCATCACATCAATCTATCATACACACACAACTTTTCAGTCACATCATCTCCAATTTCAACCAAAATCCAAACTTTGTACTGAGCTATACACAACCAAAATACACAATAGTGTTGTATGACGTGTGGCTTTCCCTATTTGTCAAGCAAATAGC
CDS Sequence
  • >LOC_Os01g60600.1
    ATGCAGGCGCAATCCCGCCTCGCTGCTGCCGCGAGCGGCGGCAGCGGCAGTGGAATTAGCGGGAGCGGTGGCATTAGCAGGTTGGGTGGTGGAGCGGGGGAGGAGCACGAGGCGGTGGTGAGGGAGCTGACGCGCGGGCACGAGCTGACGGCGCGGCTGCGGGCGGAGGCGCTGCGGGCGCTGCGCGGGCAGGGGCAGGCCGAGGCCACCGCTACATTCATCCTCGGGGAGGTCTCCCGCGCCTTCACCGTCTGCCTCTCCATCATGGCCAGCGCCTCCCCGTCCGCCTCGCCGCCACAACCGGACGAGACGCCGCCGGCCGACTCAGCCGTCTCGCCACCACCACCGCGCGCGGCGAGAGAGGACAACGTCCCGCGAAAGAGATTATTGACAGCCTCTCCTTACGACGATGGGTACCAGTGGAGGAAGTACGGTCAAAAGAAGATCAACAACACCAACTTCCCAAGGAGCTACTACAGGTGCAGTTACCACCGGGAGCGCAGATGCCCCGCGCAGAAGCACGTGCAGCAGCGCGACGGCGACGACGTCCCGGCGCTGCACGTCGTCGTCTACACCCACGAGCACACGTGCCTGCAGGGGGCGCCCGCCGAGCTTCCCGACGCGGCGACGAACGGTGGCGCCGCCGCCGCCGCGTCGCCGGACTACTTCCCCGCCGGCGGCGAGACGCCGTCGTCGCTGCGGCGGCTCCGGGGCGTCGGCGGCGGCGGCCTGCAGCCTCAGTTCGTGGACCACCGCGCCGCCATGGAGGAGCGCGAGCGCCAGGTGCTCGTCTCGTCCCTCGCCCGCGTGCTGCAGGGGCGGCAGTGCTACGACGACGACGACGACGACGACACCGACGTCGCCTCCCTCGGCGCCGTGCACGCGCGCGCGCCTGCGGCGGCGGCGCCGGTCGCCGCGTCGTCGTCGTCGTCCGGGCCCGTCGACGCTGCCGGCGAGGAGCTGGACGTCATGGACTACGACATGACGGACGCGCTGTTCTGGGGGCCTTTCGGCACGGACAGCAACTCGTACGACGGAAACCTGACGTCGACACGCTGTTTTGATCTAATTAATTAA
Protein Sequence
  • >LOC_Os01g60600.1
    MQAQSRLAAAASGGSGSGISGSGGISRLGGGAGEEHEAVVRELTRGHELTARLRAEALRALRGQGQAEATATFILGEVSRAFTVCLSIMASASPSASPPQPDETPPADSAVSPPPPRAAREDNVPRKRLLTASPYDDGYQWRKYGQKKINNTNFPRSYYRCSYHRERRCPAQKHVQQRDGDDVPALHVVVYTHEHTCLQGAPAELPDAATNGGAAAAASPDYFPAGGETPSSLRRLRGVGGGGLQPQFVDHRAAMEERERQVLVSSLARVLQGRQCYDDDDDDDTDVASLGAVHARAPAAAAPVAASSSSSGPVDAAGEELDVMDYDMTDALFWGPFGTDSNSYDGNLTSTRCFDLIN*