Gene Details:

• MSU gene ID: LOC_Os01g59660
• RAPdb gene ID: Os01g0812000
• Gene Symbol: OsMYBGA OsGAMYB
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Mutation of the two W boxes prevents the binding of OsWRKY71 to the mutated promoter, and releases the suppression of the OsGAMYB-activated Amy32b expression by OsWRKY71, suggesting that OsWRKY71 blocks GA signaling by functionally interfering with OsGAMYB.
• High-level OsGAMYB expression was detected in the aleurone cells, inflorescence shoot apical region, stamen primordia, and tapetum cells of the anther, but only low-level expression occurred in organs at the vegetative stage or in the elongating stem.
• No alpha-amylase expression in the endosperm was induced in these mutants in response to GA treatment, indicating that the Tos17 insertion had knocked out OsGAMYB function.
• The sequences were also required for effective transactivation by the transcription factor OsGAMYB.
• These results demonstrate that, in addition to its role in the induction of alpha-amylase in aleurone, OsGAMYB also is important for floral organ development and essential for pollen development.
• Thus, OsGAMYB and OsGAMYB-like genes are regulated by miR159 in flowers.
• Four copies of either GARE or CARE showed transactivation neither by OsGAMYB nor by GA induction.
• <U+2022> Further analysis showed that transcription factor of OsGAMYB was an activator of OsTPP1, and expression of OsGAMYB was decreased by both the exogenous and endogenous ABA, subsequently reduced the expression of OsTPP1, which suggested a new signaling pathway required for seed germination in rice.
• MiR319-targeted OsTCP21 and OsGAMYB regulate tillering and grain yield in rice.
• These data suggest that miR319 regulates rice tiller bud development and tillering through targeting OsTCP21 and OsGAMYB.
• Notably, the tiller number and grain yield increased in STTM319 and overexpression lines of OsTCP21 and OsGAMYB but decreased in OE319a and knockout lines of OsTCP21 and OsGAMYB.
• Moreover, OsTCP21 and OsGAMYB overexpression lines and STTM319 had increased tiller bud length and biomass, whereas both were decreased in OsTCP21 and OsGAMYB knockout lines and OE319a.
• Using transient dual-luciferase and yeast one-hybrid assays, IPA1 was found to directly activate the expression of OsWRKY51 and OsWRKY71, which would interfere with the binding affinity of GA-induced transcription factor OsGAMYB to inhibit the expression of -amylase genes.

Function-related keywords:

• ga ,  anther ,  tapetum ,  inflorescence ,  stem ,  transcription-factor ,  endosperm ,  pollen ,  flower ,  shoot ,  vegetative ,  stamen ,  floral ,  seed ,  seed-germination ,  tillering ,  development ,  grain ,  tiller ,  grain-yield ,  yield ,  tiller-number

Literature:

• A RING finger E3 ligase gene, Oryza sativa Delayed Seed Germination 1 (OsDSG1), controls seed germination and stress responses in rice .  DOI:  10.1007/s11103-010-9687-3 ;  PMID:  20878348
• Functional dissections between GAMYB and Dof transcription factors suggest a role for protein-protein associations in the gibberellin-mediated expression of the RAmy1A gene in the rice aleurone .  DOI:  10.1104/pp.103.027334 ;  PMID:  14500792
• Convergent starvation signals and hormone crosstalk in regulating nutrient mobilization upon germination in cereals .  DOI:  10.1105/tpc.112.097741 ;  PMID:  22773748
• Gibberellin modulates anther development in rice via the transcriptional regulation of GAMYB .  DOI:  10.1105/tpc.108.062935 ;  PMID:  19454733
• Two cis-acting elements necessary and sufficient for gibberellin-upregulated proteinase expression in rice seeds .  DOI:  10.1046/j.1365-313x.2003.01753.x ;  PMID:  12787245
• GAMYB controls different sets of genes and is differentially regulated by microRNA in aleurone cells and anthers .  DOI:  10.1111/j.1365-313X.2006.02795.x ;  PMID:  16792694
• Identification of gamyb-4 and analysis of the regulatory role of GAMYB in rice anther development .  DOI:  10.1111/j.1744-7909.2010.00959.x ;  PMID:  20590996
• Loss-of-function mutations of the rice GAMYB gene impair alpha-amylase expression in aleurone and flower development .  DOI:  10.1105/tpc.017327 ;  PMID:  14688295
• A rice WRKY gene encodes a transcriptional repressor of the gibberellin signaling pathway in aleurone cells .  DOI:  10.1104/pp.103.034967 ;  PMID:  15047897
• Rice dwarf mutant d1, which is defective in the alpha subunit of the heterotrimeric G protein, affects gibberellin signal transduction .  DOI:  10.1073/pnas.97.21.11638 ;  PMID:  11027362
• Interaction between rice MYBGA and the gibberellin response element controls tissue-specific sugar sensitivity of alpha-amylase genes .  DOI:  10.1105/tpc.105.038844 ;  PMID:  16905658
• Fine-Tuning Roles of Osa-miR159a in Rice Immunity Against Magnaporthe oryzae and Development .  DOI:  10.1186/s12284-021-00469-w ;  PMID:  33677712
• OsTPP1 regulates seed germination through the crosstalk with abscisic acid in rice .  DOI:  10.1111/nph.17300 ;  PMID:  33629374
• MiR319-targeted OsTCP21 and OsGAmyb regulate tillering and grain yield in rice .  DOI:  10.1111/jipb.13097 ;  PMID:  33838011
• IPA1 Negatively Regulates Early Rice Seedling Development by Interfering with Starch Metabolism via the GA and WRKY Pathways .  DOI:  10.3390/ijms22126605 ;  PMID:  34203082

Related News:

Gene Resources:

• NCBI ID: CAA67000
• UniProt accessions:

Sequences: