Gene Details:

Functional Descriptions:

  • Constitutive expression of OsGH3.1 reduces auxin content and enhances defense response and resistance to a fungal pathogen in rice.
  • LC1 is transcribed in various tissues and encodes OsGH3.1, an indole-3-acetic acid (IAA) amido synthetase, whose homolog of Arabidopsis functions in maintaining the auxin homeostasis by conjugating excess IAA to various amino acids.
  • Phenotypic analysis confirmed the exaggerated leaf angles of LC1-D due to the stimulated cell elongation at the lamina joint.
  • The transcript abundance of nearly all OsGH3 genes is enhanced on auxin treatment, with the effect more pronounced on OsGH3.1, -2, and -4.
  • Indeed, recombinant LC1 can catalyze the conjugation of IAA to Ala, Asp, and Asn in vitro, which is consistent with the decreased free IAA amount in LC1-D mutant.
  • LC1-D is insensitive to IAA and hypersensitive to exogenous BR, in agreement with the microarray analysis that reveals the altered transcriptions of genes involved in auxin signaling and BR biosynthesis.
  • Studies on the rice LEAF INCLINATION1 (LC1), an IAA-amido synthetase, reveal the effects of auxin in leaf inclination control.
  • Through genetic screening, a rice gain-of-function mutant leaf inclination1, LC1-D, was identified from the Shanghai T-DNA Insertion Population (SHIP).

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os01g57610.1
    CACAAACCAAGCTCCATACCGCTGCCTACATCTCTGGTTAGTAGCCACCAAGGTCCAAGGAGTAACACGCTCCGTGAGAGACCGAAATCCAGGGAGCACAAATTGCACATTTGCACCAAGCACACTCGCTGCAGCTGCAGTGACATGCCGGAAGCACCGACCGCCAAGACAGCACCGGCCTACGGCTACGCCCCCGGGGCGCACGCCGAGGCGCTCGAGTTCATCGAGCACGTCACGGCGAACGCCGGGCAGGTGCAGCGGCGCGTGCTCGGCGAGATCCTGGCGCAGAACGCGCCGGCCGAGTACCTGCGCCGGTACGGAATCCCCGGGTCCCCCGACGTTGTCGACGCCTTCCGCCGCCTCGTCCCGCTCGTCACATACGAGGGCCTCCAGCCAGACATCCTCCGCATCGCCAACGGCGACACCTCGCCGATCTTCTCCGGGAAGCCTATCTCCGAATTCCTCACGAGCTCGGGCACGTCGGGAGGGGAGAGGAAGCTCATGCCGACCATCGCCGACGAGATGAACAGGCGGTCGCTGCTGTACAGCCTGCTGATGCCGGTGATGAGCCAGTCGGTGTCCGGGCTCGACAAAGGCAAGGCGATGTACCTGCTCTTCGTGAAGGCGGAGTCGCGCACGCCGGGCGGGCTCGCGGCGCGGCCGGTGCTCACAAGCTACTACCGGAGCCGGCAGTTCCTCGACCGTCCGCGCGACCCCTACACATCTTACACGAGCCCCGACGAGGCCATCCTGTGCGTGGACTCCTACCAGAGCATGTACGCGCAGCTGCTCTGCGGCCTCGTCCACCGCGCCGACGTGCTGCGCGTGGGCGCCGTGTTCGCCTCCGGCTTCCTCCGCGCCATCCATTTCCTCGAGAAGCACTGGGCGCGCCTCTGCCACGACATCCGCACCGGCGAGCTCGACCCGGAGATCACCGACCGCGTGGTGCGCGACGCCGTCGGGCGGGTGCTCCGCGCCGACCCGGCGCTCGCCGACGCGATCGAGGACGAGTGCGCTAGGGCGTCGTGGGAGGGCATCATCCGGCGCCTGTGGCCACGCACCAAGTACATCGACGTGATCGTGACCGGCACCATGTCGCAGTACATCCCGACGCTCGAGTTCTACGGCGGCGGCCTGCCGCTGACGTGCACCATGTACGCCTCTTCGGAGTGCTACTTCGGCCTCAACCTGAATCCCATGTGCAAGCCCAGCGACGTCGCCTACACGCTCATCCCCACCATGTGCTACTACGAGTTCCTCCCCGTCAATTGCAACAATGCCACTGCCGAGGCGAGCCACCGCGACCTCGTCGACCTGGTCGACGTAAAGCTCGGGCACGAGTACGAGCTCGTGGTCACCACGTATTCCGGGTTGTATCGTTATCGCGTGGGCGACGTGCTGAGGGTGGCGGGGTTCAAGAACAAGGCCCCGATGTTCAGCTTCGTGCGGCGGCAGAACGTGGCGCTGAGCGTCGACTCGGACAAGACGGACGAGACGGAGCTGCACGCGGCGGTGAGCGGCGCGGTGCAGCACCTGGCGCCGTTCGGCGCGTCGCTGGTGGAGTACACGAGCTACGCGGACGCGGCCACCATCCCGGGCCACTACGTGCTGTTCTGGGAGCTGCGCGCCGGCAGCACGGCGGTGCCGGCGTCCGTGTTCGAGGAGTGCTGCCTGTCCGTGGAGGAGGCACTGAACAGCGTCTACCGGCAGGGCCGCGCGTGCGACAGGTCCATCGGCCCGCTCGAGATACGCGTCGTGGCGGAGGGCACCTTCGACAAGCTCATGGACTACGCGATCAGCCGGGGCGCGTCCATCAACCAGTACAAGGCGCCGCGGTGCGTGCGCCCTGGCCCGGTCGTCGAGCTGCTCGACGCGAGGGTGCAGGGCAAGTACTTCAGTCCCAAGTGCCCCAAGTGGAGCCCCGGGAACAAGCAATGGAACAAAAGCAAGGATCTGGTCGGCAAGGGAGACGCCTAATATTGAAGCTAGAGGGTGATGATCTGATCGAGTATTTGTGTGAGCTGGTAATTAATTAACGGTACATGCATATGGTCATTGGTCGATATGTTTCTTTAGGGATATGATAATTCATGTTTGAATTATCTCTGATGCTGCGAGAGGATCGAATAGTTATGAGATGACGACTTAGCCTATATGATCATTTGCTTTACTCTTTCTCTGCTGTAACGCAGTATATGTTATTTCCTCCGTCTCAAAATATAAGCATTTTTAGTACATTGATAAATTAAACATTTTTAAATTTGACTATTAATAGCAAGAAAAATAAAAAATCAATCATGTAAAATTGATATTCCTAGATTTATCATTAAAAACACTATCATAATATGCAACTTTTTTTATTTAAAATATTTTATTTTTATATATATTGTTGGTTAAAGTAGCATCTTGGATATCGTGTCAGGGTTTAAAATGATTATATTTTAGGACGGAGGGAGTAATTAGTAGCCGTATTGAATAGACTTTTCTACTCAAAGATATGTCTCTCAAATTTATGCACATCGCACGGTGTGCATGCAGTGCCGAGAATTTATTACTTGTCAAAATTGTTGGACTACTGCTACTGATGATAAATAACTTGTCAAAATGATAAACTACATCAAATACTACTATAGTAGTATATTATAATCTTCCTTTATACTAGTATATGCTATCTCATGATGGTTGACATTAA
CDS Sequence
  • >LOC_Os01g57610.1
    ATGCCGGAAGCACCGACCGCCAAGACAGCACCGGCCTACGGCTACGCCCCCGGGGCGCACGCCGAGGCGCTCGAGTTCATCGAGCACGTCACGGCGAACGCCGGGCAGGTGCAGCGGCGCGTGCTCGGCGAGATCCTGGCGCAGAACGCGCCGGCCGAGTACCTGCGCCGGTACGGAATCCCCGGGTCCCCCGACGTTGTCGACGCCTTCCGCCGCCTCGTCCCGCTCGTCACATACGAGGGCCTCCAGCCAGACATCCTCCGCATCGCCAACGGCGACACCTCGCCGATCTTCTCCGGGAAGCCTATCTCCGAATTCCTCACGAGCTCGGGCACGTCGGGAGGGGAGAGGAAGCTCATGCCGACCATCGCCGACGAGATGAACAGGCGGTCGCTGCTGTACAGCCTGCTGATGCCGGTGATGAGCCAGTCGGTGTCCGGGCTCGACAAAGGCAAGGCGATGTACCTGCTCTTCGTGAAGGCGGAGTCGCGCACGCCGGGCGGGCTCGCGGCGCGGCCGGTGCTCACAAGCTACTACCGGAGCCGGCAGTTCCTCGACCGTCCGCGCGACCCCTACACATCTTACACGAGCCCCGACGAGGCCATCCTGTGCGTGGACTCCTACCAGAGCATGTACGCGCAGCTGCTCTGCGGCCTCGTCCACCGCGCCGACGTGCTGCGCGTGGGCGCCGTGTTCGCCTCCGGCTTCCTCCGCGCCATCCATTTCCTCGAGAAGCACTGGGCGCGCCTCTGCCACGACATCCGCACCGGCGAGCTCGACCCGGAGATCACCGACCGCGTGGTGCGCGACGCCGTCGGGCGGGTGCTCCGCGCCGACCCGGCGCTCGCCGACGCGATCGAGGACGAGTGCGCTAGGGCGTCGTGGGAGGGCATCATCCGGCGCCTGTGGCCACGCACCAAGTACATCGACGTGATCGTGACCGGCACCATGTCGCAGTACATCCCGACGCTCGAGTTCTACGGCGGCGGCCTGCCGCTGACGTGCACCATGTACGCCTCTTCGGAGTGCTACTTCGGCCTCAACCTGAATCCCATGTGCAAGCCCAGCGACGTCGCCTACACGCTCATCCCCACCATGTGCTACTACGAGTTCCTCCCCGTCAATTGCAACAATGCCACTGCCGAGGCGAGCCACCGCGACCTCGTCGACCTGGTCGACGTAAAGCTCGGGCACGAGTACGAGCTCGTGGTCACCACGTATTCCGGGTTGTATCGTTATCGCGTGGGCGACGTGCTGAGGGTGGCGGGGTTCAAGAACAAGGCCCCGATGTTCAGCTTCGTGCGGCGGCAGAACGTGGCGCTGAGCGTCGACTCGGACAAGACGGACGAGACGGAGCTGCACGCGGCGGTGAGCGGCGCGGTGCAGCACCTGGCGCCGTTCGGCGCGTCGCTGGTGGAGTACACGAGCTACGCGGACGCGGCCACCATCCCGGGCCACTACGTGCTGTTCTGGGAGCTGCGCGCCGGCAGCACGGCGGTGCCGGCGTCCGTGTTCGAGGAGTGCTGCCTGTCCGTGGAGGAGGCACTGAACAGCGTCTACCGGCAGGGCCGCGCGTGCGACAGGTCCATCGGCCCGCTCGAGATACGCGTCGTGGCGGAGGGCACCTTCGACAAGCTCATGGACTACGCGATCAGCCGGGGCGCGTCCATCAACCAGTACAAGGCGCCGCGGTGCGTGCGCCCTGGCCCGGTCGTCGAGCTGCTCGACGCGAGGGTGCAGGGCAAGTACTTCAGTCCCAAGTGCCCCAAGTGGAGCCCCGGGAACAAGCAATGGAACAAAAGCAAGGATCTGGTCGGCAAGGGAGACGCCTAA
Protein Sequence
  • >LOC_Os01g57610.1
    MPEAPTAKTAPAYGYAPGAHAEALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEGLQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVMSQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEAILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDPEITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYIPTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATAEASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWELRAGSTAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGASINQYKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSPGNKQWNKSKDLVGKGDA*