OsWRKY13;WRKY13

Gene Details

MSU gene ID: LOC_Os01g54600
RAPdb gene ID: Os01g0750100
Gene Symbol: OsWRKY13 WRKY13
Genome: MSU7 , IRGSP-1.0
Species: Oryza sativa

Functional Descriptions

Furthermore, OsWRKY13 will provide a transgenic tool for engineering wider-spectrum and whole-growth-stage resistance rice in breeding programs.
At least 39 transcription factor genes were influenced by OsWRKY13, and 30 of them were downregulated.
The promoters of OsWRKY13-upregulated genes were overrepresented with W-boxes for WRKY protein binding, whereas the promoters of OsWRKY13-downregulated genes were enriched with cis-elements putatively for binding of MYB and AP2/EREBP types of transcription factors.
CONCLUSION: These results suggest that OsWRKY13-mediated signalling pathways are partitioned by different transcription factors.
In this study, we identified cis-acting elements and their binding proteins of rice OsWRKY13, a gene that plays a pivotal role in disease resistance against bacterial and fungal pathogens.
These results suggest that OsWRKY13 expression is regulated by multiple factors to achieve disease resistance.
Identification of novel pathogen-responsive cis-elements and their binding proteins in the promoter of OsWRKY13, a gene regulating rice disease resistance.
BACKGROUND: Rice transcription regulator OsWRKY13 influences the functioning of more than 500 genes in multiple signalling pathways, with roles in disease resistance, redox homeostasis, abiotic stress responses, and development.
Here, we show that OsWRKY13 plays a pivotal role in rice disease resistance.
OsWRKY13 mediates rice disease resistance by regulating defense-related genes in salicylate- and jasmonate-dependent signaling.
Overexpression of OsWRKY13 can enhance rice resistance to bacterial blight and fungal blast, two of the most devastating diseases of rice worldwide, at both the seedling and adult stages, and shows no influence on the fertility.
The OsWRKY13-associated disease resistance pathway synergistically interacts via OsWRKY13 with the glutathione/glutaredoxin system and flavonoid biosynthesis pathway to monitor redox homeostasis and to putatively enhance the biosynthesis of antimicrobial flavonoid phytoalexins, respectively, in OsWRKY13-overexpressing lines.
Meanwhile, the OsWRKY13-associated disease resistance pathway appears to interact antagonistically with the SNAC1-mediated abiotic stress defense pathway, jasmonic acid signaling pathway, and terpenoid metabolism pathway via OsWRKY13 to suppress salt and cold defense responses as well as to putatively retard rice growth and development.
Rice gene network inferred from expression profiling of plants overexpressing OsWRKY13, a positive regulator of disease resistance.
These results suggest that OsWRKY13, as an activator of the SA-dependent pathway and a suppressor of JA-dependent pathways, mediates rice resistance by directly or indirectly regulating the expression of a subset of genes acting both upstream and downstream of SA and JA.
Here we report that OsWRKY13, an activator of rice resistance to both bacterial and fungal pathogens, appears to function as a convergent point for crosstalk among the pathogen-induced salicylate-dependent defense pathway and five other physiologic pathways.
WRKY45-2, functioning as a transcriptional activator, directly activated WRKY13.
Previous studies have revealed that rice WRKY13 (as a transcriptional repressor) and WRKY45-2 enhance resistance to M.
To compute and evaluate the possible molecular mechanism for observed resistance correlated to WRKY13 gene expression, rice gene expression profiles against bacterial leaf blight and leaf blast disease from ROAD database were used to prioritize the locus IDs that were used as input in RiceNet v2 tool.
Gene network mediated by WRKY13 to regulate resistance against sheath infecting fungi in rice (Oryza sativa L.).
Agrobacterium mediated transformation was carried out in rice plants using overexpression construct of WRKY13 (agroinfection in seeds of varieties susceptible to sheath blight and sheath rot, followed by selection in antibiotic media, germinating and hardening of putative transgenic lines).
RT-PCR and qPCR analyses of WRKY13 using RNA extracted from the plant tissues revealed higher WRKY13 expression in resistant varieties (both diseases) upon pathogen challenge compared to uninfected control and also the susceptible varieties.

Functional Keywords

Literature and News

Gene Resources

Sequences

cDNA Sequence

>LOC_Os01g54600.1
ATGTGCCGCGCGCCGGTGTAGCGCAGCGTAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTATGGTAGTCTTCCTACGCTCGGTCAGTGACAGCGTCGCTGCGCATAAAGAAAGGCATCCACTAGCAGGAGCCAGCATACGCTGCCGCATATACAAACACGCATGTAGCCAAGGCGAGGAGAGAGAGAGAGAGAGAAAGGGAGATCATTGAAAGCTGCACCCATCCGTTTGTTCGCCACCATTTCCATCCCCTCCATCATGAGCTCCAATTAGCCGCACACCGATCCCCTCCTCTTCTTCCCCCCCTTCTCCTCTCTCCTCTTTTTTTCCTCATCCGTGCGCGATACTGCGCTTAGTTTGTGGGTGGGGGGAGTGATCATCACCAAAGCCACAAAGGGGGCGCGGAGAGATATTATATATTCTTTGGGAAAGCGTTGGATTAGTTTTTCTCGCTTTGGGCTTTTTATCCGGAGTTGGAGTGTGTGTGCGCCGGGAGTGGTGGTGGTGATGGCGGCCGGAGAGGAGGTGATGGATCGGTCGACGTCGGCCGAGGACGGGTACTGCAGCGCCGGCACGGACTCGCCGCGGGCGGAGTCCGTCGACGAGCAAGGGGCCGCCGAGGAGTCGTCGCCCCGGGGAGGGCAGAAGCGGGAGCTCCCCTCGCCGTCGGCTTCGCCGTCGTCCCCGCTGCCACCTGCCGCGAAGCGCAGCCGGAGGTCAGTGGAGAAGCGGGTGGTGTCCGTGCCGATCGCGGAGTGCGGCGACCGGCCGAAGGGCGCCGGGGAGGGGCCGCCGCCGTCGGACTCGTGGGCCTGGCGCAAGTACGGCCAGAAGCCCATCAAGGGCTCTCCCTACCCAAGGGGGTACTACAGGTGCAGTAGCTCCAAGGGGTGTCCGGCGAGGAAGCAGGTGGAGCGCAGCCGCGCCGACCCCACCGTGCTGCTCGTCACCTACTCCTTCGAGCACAACCACCCGTGGCCGCAGCCCAAGAGCAGCAGCTGCCACGCGAGCAAGTCGTCTCCCCGGTCGACGGCGCCAAAGCCTGAGCCGGTGGCTGACGGACAGCACCCCGAGCCAGCGGAGAACGAATCGTCGGCGTCGGCGGAGCTGGAGGTACCGGAGCCGGAGCCGGAGCAGGAATCTGAGCCGGTTGTGAAGCAGGAGGAGGAGCAGAAGGAGGAACAGAAGGCTGTGGTTGAGCCGGCGGCGGTCACAACCACGGTGGCGCCGGCGCCGGCGGTCGAGGAGGAGGATGAGAACTTCGACTTCGGATGGATCGACCAGTACCACCCGACGTGGCACCGGTCGTACGCGCCGCTGCTGCCGCCGGAGGAGTGGGAGCGCGAGCTGCAAGGGGACGACGCGCTGTTCGCGGGGCTCGGCGAGCTCCCGGAGTGCGCCGTCGTGTTCGGCCGCCGGCGCGAGCTCGGCCTGGCGGCCACCGCGCCGTGCTCCTGATGATCACCTCCTCTTTTCTTTTTCCTCCCCTTTTACAATTTCCCTTTTTTTTTTTTGCTTTTCTTTAGTTCTCCTTGGAGTAATTTGGATTGGAGGTTAGTACATAGCTTGGGTGATCAATGGGAGTGAGTGGCTTAACTGTTCAAGAGAGCCTCCTATTCCCACTCTCCAAATTGCGTCGCGTGTTCGTCATCCAAAGCCCGTCGTGCCGTTTCCGAAGTGGTTGGCGACGTGATTCCAACGACAAAAAAGCAGCGAAAGGTGGGCATTTT

CDS Sequence

>LOC_Os01g54600.1
ATGGCGGCCGGAGAGGAGGTGATGGATCGGTCGACGTCGGCCGAGGACGGGTACTGCAGCGCCGGCACGGACTCGCCGCGGGCGGAGTCCGTCGACGAGCAAGGGGCCGCCGAGGAGTCGTCGCCCCGGGGAGGGCAGAAGCGGGAGCTCCCCTCGCCGTCGGCTTCGCCGTCGTCCCCGCTGCCACCTGCCGCGAAGCGCAGCCGGAGGTCAGTGGAGAAGCGGGTGGTGTCCGTGCCGATCGCGGAGTGCGGCGACCGGCCGAAGGGCGCCGGGGAGGGGCCGCCGCCGTCGGACTCGTGGGCCTGGCGCAAGTACGGCCAGAAGCCCATCAAGGGCTCTCCCTACCCAAGGGGGTACTACAGGTGCAGTAGCTCCAAGGGGTGTCCGGCGAGGAAGCAGGTGGAGCGCAGCCGCGCCGACCCCACCGTGCTGCTCGTCACCTACTCCTTCGAGCACAACCACCCGTGGCCGCAGCCCAAGAGCAGCAGCTGCCACGCGAGCAAGTCGTCTCCCCGGTCGACGGCGCCAAAGCCTGAGCCGGTGGCTGACGGACAGCACCCCGAGCCAGCGGAGAACGAATCGTCGGCGTCGGCGGAGCTGGAGGTACCGGAGCCGGAGCCGGAGCAGGAATCTGAGCCGGTTGTGAAGCAGGAGGAGGAGCAGAAGGAGGAACAGAAGGCTGTGGTTGAGCCGGCGGCGGTCACAACCACGGTGGCGCCGGCGCCGGCGGTCGAGGAGGAGGATGAGAACTTCGACTTCGGATGGATCGACCAGTACCACCCGACGTGGCACCGGTCGTACGCGCCGCTGCTGCCGCCGGAGGAGTGGGAGCGCGAGCTGCAAGGGGACGACGCGCTGTTCGCGGGGCTCGGCGAGCTCCCGGAGTGCGCCGTCGTGTTCGGCCGCCGGCGCGAGCTCGGCCTGGCGGCCACCGCGCCGTGCTCCTGA

Protein Sequence

>LOC_Os01g54600.1
MAAGEEVMDRSTSAEDGYCSAGTDSPRAESVDEQGAAEESSPRGGQKRELPSPSASPSSPLPPAAKRSRRSVEKRVVSVPIAECGDRPKGAGEGPPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRADPTVLLVTYSFEHNHPWPQPKSSSCHASKSSPRSTAPKPEPVADGQHPEPAENESSASAELEVPEPEPEQESEPVVKQEEEQKEEQKAVVEPAAVTTTVAPAPAVEEEDENFDFGWIDQYHPTWHRSYAPLLPPEEWERELQGDDALFAGLGELPECAVVFGRRRELGLAATAPCS*