Gene Details:

• MSU gene ID: LOC_Os01g54270
• RAPdb gene ID: Os01g0746400
• Gene Symbol: D10 OsCCD8 OsCCD8b
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Besides, in D10-RNAi plants, decreased storage cytokinin levels in the shoot node may partly account for the increased active cytokinin contents, resulting in more tillering phenotypes.
• Real-time polymerase chain reaction analysis revealed that accumulation of D10 mRNA is induced by exogenous auxin.
• D10 is a rice ortholog of MAX4/RMS1/DAD1 that encodes a carotenoid cleavage dioxygenase 8 and is supposed to be involved in the synthesis of an unidentified inhibitor of shoot branching.
• We discovered a triazole-type chemical, TIS13 [2,2-dimethyl-7-phenoxy-4-(1H-1,2,4-triazol-1-yl)heptan-3-ol], which induced outgrowth of second tiller buds of wild-type seedlings, as observed for non-treated strigolactone-deficient D10 mutant seedlings.
• Moreover, D10 expression is upregulated in six branching mutants, d3, D10, d14, d17, d27 and high tillering dwarf (htd1).
• Here we use D10- RNA interference (RNAi) transgenic plants similar to D10 mutant in phenotypes to investigate the interactions among D10, auxin and cytokinin in regulating rice shoot branching.
• Auxin levels in node 1 of both decapitated D10-RNAi and wild type plants decreased significantly, showing that decapitation does reduce endogenous auxin concentration, but decapitation has no clear effects on auxin levels in node 2 of the same plants.
• This implies that node 1 may be the location where a possible interaction between auxin and D10 gene would be detected.
• D10 expression in node 1 is inhibited by decapitation, and this inhibition can be restored by exogenous auxin application, indicating that D10 may play an important role in auxin regulation of SL.
• The decreased expression of most OsPINs in shoot nodes of D10-RNAi plants may cause a reduced auxin transport capacity.
• Furthermore, effects of auxin treatment of decapitated plants on the expression of cytokinin biosynthetic genes suggest that D10 promotes cytokinin biosynthesis by reducing auxin levels.
• In dwarf10 (D10), an enhanced branching mutant of rice, apical dominance can be observed, but the inhibitory effects of the apical meristem was reduced.
• The d14 mutant exhibits increased shoot branch-ing with reduced plant height like the previously characterized strigolactone-deficient and -insensitive mutants D10 and d3, respectively.
• However, unlike with D10, the d14 branching phenotype could not be rescued by exogenous strigolactones.
• Loss of function of HTD2 resulted in a significantly increased expression of HTD1, D10 and D3, which were involved in the strigolactone biosynthetic pathway.
• Moreover, the mRNA levels of D3, HTD1 and D10, which are orthologs of Arabidopsis MAX2/ORE9, MAX3 and MAX4, respectively, increased during cell death.
• Previous studies have shown that DWARF10 (D10) is a rice ortholog of MAX4/RMS1/DAD1, encoding a carotenoid cleavage dioxygenase and functioning in strigolactones/strigolactone-derivatives (SL) biosynthesis.
• These findings imply that D10 transcription might be a critical step in the regulation of the branching inhibitor pathway.

Function-related keywords:

• tillering ,  auxin ,  shoot ,  growth ,  tiller ,  meristem ,  branching ,  strigolactone ,  cell-death ,  dwarf ,  cytokinin ,  height ,  seedling

Literature:

• Identification and characterization of HTD2: a novel gene negatively regulating tiller bud outgrowth in rice .  DOI:  10.1007/s00425-009-0975-6 ;  PMID:  19579033
• Rice tillering dwarf mutant dwarf3 has increased leaf longevity during darkness-induced senescence or hydrogen peroxide-induced cell death .  DOI:  10.1266/ggs.82.361 ;  PMID:  17895586
• The rice HIGH-TILLERING DWARF1 encoding an ortholog of Arabidopsis MAX3 is required for negative regulation of the outgrowth of axillary buds .  DOI:  10.1111/j.1365-313X.2006.02916.x ;  PMID:  17092317
• DWARF10, an RMS1/MAX4/DAD1 ortholog, controls lateral bud outgrowth in rice .  DOI:  10.1111/j.1365-313X.2007.03210.x ;  PMID:  17655651
• The interactions among DWARF10, auxin and cytokinin underlie lateral bud outgrowth in rice .  DOI:  10.1111/j.1744-7909.2010.00960.x ;  PMID:  20590993
• A new lead chemical for strigolactone biosynthesis inhibitors .  DOI:  10.1093/pcp/pcq077 ;  PMID:  20522488
• d14, a strigolactone-insensitive mutant of rice, shows an accelerated outgrowth of tillers .  DOI:  10.1093/pcp/pcp091 ;  PMID:  19542179
• Carotenoid oxygenases involved in plant branching catalyse a highly specific conserved apocarotenoid cleavage reaction .  DOI:  10.1042/BJ20080568 ;  PMID:  18637791
• Characterization of dwarf and narrow leaf (dnl-4) mutant in rice .  DOI:  10.1080/15592324.2020.1849490 ;  PMID:  33300429

Related News:

Gene Resources:

• NCBI ID: NM_191187
• UniProt accessions:

Sequences: