Gene Details:

Functional Descriptions:

  • The MSI1 member OsRBAP1 gene, identified by a modified MutMap method, is required for rice height and spikelet fertility.
  • Altogether, our results suggest that OsRBAP1 plays an important role in the regulation of rice height and spikelet fertility.
  • The CRISPR/Cas9 system was used to switch off OsRBAP1 in PTWT to obtain the knockout mutant OsRBAP1, which exhibited a severe reduction in height and fertility.
  • MutMap-based cloning revealed the occurrence of a single amino acid substitution in the LOC_Os01g51300 gene, belonging to the MSI1 (multicopy suppressor of IRA1) member OsRBAP1.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os01g51300.1
    ATTCGGGCACAAGAGGGGTGGCATCTCTGAGCGGGAGCCACGCGCAAGGGTCCAGAGCAAACGCTCCTCATACGACAGAGAGAGCGTGACAGCGCGAGGGGGTTCGGAGGCGGGAGCGGGCGACGGCGGCGGCGGCGGGCGCCAGCGAGCGGCGAAGGGCGCGCTGGAGATGAAGGAGAAGGGGTCGAGGGCGGCGGTGGACGAGCGCTACGCGCAGTGGAAGTCGCTCATCCCGGTGCTCTACGACTGGTTCGCCAACCACAACCTCGTCTGGCCGTCCCTCTCCTGCCGGTGGGGACCCCAGTTTGAGAAGGCTACCTACAAGAATCGTCAGCGTCTTTACCTTTCAGAGCAGACGGATGGGACTGTCCCTAATACTCTGGTTATTGCAAATTGTGAAGTTGTCAAACCAAGGGTTGCAGCTGCTGAACATATATCTCAGTTCAATGAGGAAGCACGATCACCTTTTGTAAAGAAGTACAAGACTATAATTCATCCAGGCGAGGTGAACAGGATTAGGGAGCTTCCGCAGAACAGTAAGATCATTGCCACTCATACCGACAGCCCAGATGTTCTCATTTGGGATGTCGAGGCCCAGCCAAATAGACAAGCTCAACTGGCACAGATGGAGTCTCGTCCTGATTTGGTACCACCTGACTCTCGCCCTGATTTGATATTAAGAGGACATAAGGATATTGCTGAGTTTGCGCTTGCTATGTGCCCAGCTGAGCCATATGTGTTATCTGGAGGAAAAGACAAATCTGTTGTATGGTGGAGCATCCAAGACCACATATCTGCACTGGGAGATTCCTCGAAAACTGAATCTTCTCCAGGGGCATCAGGAAGCAAGGGAAAAACTGCAAATGATAAGGATAGTCCTAAAGTTGATCCTCGTGGTATCTTTCTTGGCCACGACAGTACTGTTGAAGATGTCCAGTTCTGCCCTTCTAGTGCACAGGAGTTTTGTAGTGTAGGCGATGATTCTTGTCTTATTCTTTGGGATGCCCGGAGTGGTACAGGCCCAGCTGTTAAGGTTGAGAAAGCTCATGGTGGTGATGTTCATTGTGTTGACTGGAACCTCCATGATGTTAACTATATCTTAACTGGTTCTGCGGATAATTCTGTCCGTATGTGGGACCGGAGAAATCTGGGTTCTGGAGGAGCTGGCATTCCAGTTCACAAATTTGAGGGTCATAAAGCTGCTGTTCTTTGTGTTCAGTGGTCACCTGACAAGGCATCTGTATTTGGAAGCTCTGCGGAAGACGGCTTCTTAAATGTGTGGGATCATGAGAAGGTTGGAAATAAGAAAAATCCCAACGCACCTGCTGGGCTTTTCTTTCAACATGCTGGTCATAGGGATAAGATTGTAGACTTCCACTGGAATTCTTCGGATCCTTGGACTATTGTGAGTGTGTCTGATGATGGTGAGAGTACTGGTGGAGGTGGAACATTGCAGATATGGCGCATGAGTGACCTGATATACCGTCCAGAAGATGAAGTTCTTGCAGAGCTGGAGAACTTCAAGACTCACTTGGCCAGCTGTGCTCCAAGGAGCTGAATCGTGTGACTATCTGTGTAACCCAGTCCTGTAATAACTCTAGTAGGTTTCTAATTTCTTGTCCATTTAGAATGTTCCTTTCAAATATTGGTTATATCCTCTGGTGCGGAACCCAGGCTTGTACCCTTCAAGTGCTTGTGCGACTCTTTTACCTTACTGTATCGACTCAAATCGGTAAGCTAGTGCAAACACAGCAGAGCAGAATCTCTGTGGGCAGATTATTTGGATGCTAAACATTGTTCTGGAGTCTCGTATTGGACCTAGTTGTATTGTTAGCCTGACTTGGTGACTTAATCAGATGGTTCTTCAGATAGA
  • >LOC_Os01g51300.2
    ATTCGGGCACAAGAGGGGTGGCATCTCTGAGCGGGAGCCACGCGCAAGGGTCCAGAGCAAACGCTCCTCATACGACAGAGAGAGCGTGACAGCGCGAGGGGGTTCGGAGGCGGGAGCGGGCGACGGCGGCGGCGGCGGGCGCCAGCGAGCGGCGAAGGGCGCGCTGGAGATGAAGGAGAAGGGGTCGAGGGCGGCGGTGGACGAGCGCTACGCGCAGTGGAAGTCGCTCATCCCGGTGCTCTACGACTGGTTCGCCAACCACAACCTCGTCTGGCCGTCCCTCTCCTGCCGGTGGGGACCCCAGTTTGAGAAGGCTACCTACAAGAATCGTCAGCGTCTTTACCTTTCAGAGCAGACGGATGGGACTGTCCCTAATACTCTGGTTATTGCAAATTGTGAAGTTGTCAAACCAAGGGTTGCAGCTGCTGAACATATATCTCAGTTCAATGAGGAAGCACGATCACCTTTTGTAAAGAAGTACAAGACTATAATTCATCCAGGCGAGGTGAACAGGATTAGGGAGCTTCCGCAGAACAGTAAGATCATTGCCACTCATACCGACAGCCCAGATGTTCTCATTTGGGATGTCGAGGCCCAGCCAAATAGACAAGCTCAACTGGCACAGATGGAGTCTCGTCCTGATTTGGTACCACCTGACTCTCGCCCTGATTTGATATTAAGAGGACATAAGGATATTGCTGAGTTTGCGCTTGCTATGTGCCCAGCTGAGCCATATGTGTTATCTGGAGGAAAAGACAAATCTGTTGTATGGTGGAGCATCCAAGACCACATATCTGCACTGGGAGATTCCTCGAAAACTGAATCTTCTCCAGGGGCATCAGGAAGCAAGGGAAAAACTGCAAATGATAAGGATAGTCCTAAAGTTGATCCTCGTGGTATCTTTCTTGGCCACGACAGTACTGTTGAAGATGTCCAGTTCTGCCCTTCTAGTGCACAGGAGTTTTGTAGTGTAGGCGATGATTCTTGTCTTATTCTTTGGGATGCCCGGAGTGGTACAGGCCCAGCTGTTAAGGTTGAGAAAGCTCATGGTGGTGATGTTCATTGTGTTGACTGGAACCTCCATGATGTTAACTATATCTTAACTGGTTCTGCGGATAATTCTGTCCGTATGTGGGACCGGAGAAATCTGGGTTCTGGAGGAGCTGGCATTCCAGTTCACAAATTTGAGGGTCATAAAGCTGCTGTTCTTTGTGTTCAGTGGTCACCTGACAAGGCATCTGTATTTGGAAGCTCTGCGGAAGACGGCTTCTTAAATGTGTGGGATCATGAGAAGGTTGGAAATAAGAAAAATCCCAACGCACCTGCTGGGCTTTTCTTTCAACATGCTGGTCATAGGGATAAGATTGTAGACTTCCACTGGAATTCTTCGGATCCTTGGACTATTGTGAGTGTGTCTGATGATGGTGAGAGTACTGGTGGAGGTGGAACATTGCAGATATGGCGCATGAGTGACCTGATATACCGTCCAGAAGATGAAGTTCTTGCAGAGCTGGAGAACTTCAAGACTCACTTGGCCAGCTGTGCTCCAAGGAGCTGAATCGTGTGACTATCTGTGTAACCCAGTCCTGTAATAACTCTAGTAGGTTTCTAATTTCTTGTCCATTTAGAATGTTCCTTTCAAATATTGGTTATATCCTCTGGTGCGGAACCCAGGCTTGTACCCTTCAAGTGCTTGTGCGACTCTTTTACCTTACTGTATCGACTCAAATCGGTAAGCTAGTGCAAACACAGCAGAGCAGAATCTCTGTGGGCAGATTATTTGGATGCTAAACATTGTTCTGGAGTCTCGTATTGGACCTAGTTGTATTGTTAGCCTGACTTGGTGACTTAATCAGATGGTTCTTCAGATAGA
CDS Sequence
  • >LOC_Os01g51300.1
    ATGAAGGAGAAGGGGTCGAGGGCGGCGGTGGACGAGCGCTACGCGCAGTGGAAGTCGCTCATCCCGGTGCTCTACGACTGGTTCGCCAACCACAACCTCGTCTGGCCGTCCCTCTCCTGCCGGTGGGGACCCCAGTTTGAGAAGGCTACCTACAAGAATCGTCAGCGTCTTTACCTTTCAGAGCAGACGGATGGGACTGTCCCTAATACTCTGGTTATTGCAAATTGTGAAGTTGTCAAACCAAGGGTTGCAGCTGCTGAACATATATCTCAGTTCAATGAGGAAGCACGATCACCTTTTGTAAAGAAGTACAAGACTATAATTCATCCAGGCGAGGTGAACAGGATTAGGGAGCTTCCGCAGAACAGTAAGATCATTGCCACTCATACCGACAGCCCAGATGTTCTCATTTGGGATGTCGAGGCCCAGCCAAATAGACAAGCTCAACTGGCACAGATGGAGTCTCGTCCTGATTTGGTACCACCTGACTCTCGCCCTGATTTGATATTAAGAGGACATAAGGATATTGCTGAGTTTGCGCTTGCTATGTGCCCAGCTGAGCCATATGTGTTATCTGGAGGAAAAGACAAATCTGTTGTATGGTGGAGCATCCAAGACCACATATCTGCACTGGGAGATTCCTCGAAAACTGAATCTTCTCCAGGGGCATCAGGAAGCAAGGGAAAAACTGCAAATGATAAGGATAGTCCTAAAGTTGATCCTCGTGGTATCTTTCTTGGCCACGACAGTACTGTTGAAGATGTCCAGTTCTGCCCTTCTAGTGCACAGGAGTTTTGTAGTGTAGGCGATGATTCTTGTCTTATTCTTTGGGATGCCCGGAGTGGTACAGGCCCAGCTGTTAAGGTTGAGAAAGCTCATGGTGGTGATGTTCATTGTGTTGACTGGAACCTCCATGATGTTAACTATATCTTAACTGGTTCTGCGGATAATTCTGTCCGTATGTGGGACCGGAGAAATCTGGGTTCTGGAGGAGCTGGCATTCCAGTTCACAAATTTGAGGGTCATAAAGCTGCTGTTCTTTGTGTTCAGTGGTCACCTGACAAGGCATCTGTATTTGGAAGCTCTGCGGAAGACGGCTTCTTAAATGTGTGGGATCATGAGAAGGTTGGAAATAAGAAAAATCCCAACGCACCTGCTGGGCTTTTCTTTCAACATGCTGGTCATAGGGATAAGATTGTAGACTTCCACTGGAATTCTTCGGATCCTTGGACTATTGTGAGTGTGTCTGATGATGGTGAGAGTACTGGTGGAGGTGGAACATTGCAGATATGGCGCATGAGTGACCTGATATACCGTCCAGAAGATGAAGTTCTTGCAGAGCTGGAGAACTTCAAGACTCACTTGGCCAGCTGTGCTCCAAGGAGCTGA
  • >LOC_Os01g51300.2
    ATGAAGGAGAAGGGGTCGAGGGCGGCGGTGGACGAGCGCTACGCGCAGTGGAAGTCGCTCATCCCGGTGCTCTACGACTGGTTCGCCAACCACAACCTCGTCTGGCCGTCCCTCTCCTGCCGGTGGGGACCCCAGTTTGAGAAGGCTACCTACAAGAATCGTCAGCGTCTTTACCTTTCAGAGCAGACGGATGGGACTGTCCCTAATACTCTGGTTATTGCAAATTGTGAAGTTGTCAAACCAAGGGTTGCAGCTGCTGAACATATATCTCAGTTCAATGAGGAAGCACGATCACCTTTTGTAAAGAAGTACAAGACTATAATTCATCCAGGCGAGGTGAACAGGATTAGGGAGCTTCCGCAGAACAGTAAGATCATTGCCACTCATACCGACAGCCCAGATGTTCTCATTTGGGATGTCGAGGCCCAGCCAAATAGACAAGCTCAACTGGCACAGATGGAGTCTCGTCCTGATTTGGTACCACCTGACTCTCGCCCTGATTTGATATTAAGAGGACATAAGGATATTGCTGAGTTTGCGCTTGCTATGTGCCCAGCTGAGCCATATGTGTTATCTGGAGGAAAAGACAAATCTGTTGTATGGTGGAGCATCCAAGACCACATATCTGCACTGGGAGATTCCTCGAAAACTGAATCTTCTCCAGGGGCATCAGGAAGCAAGGGAAAAACTGCAAATGATAAGGATAGTCCTAAAGTTGATCCTCGTGGTATCTTTCTTGGCCACGACAGTACTGTTGAAGATGTCCAGTTCTGCCCTTCTAGTGCACAGGAGTTTTGTAGTGTAGGCGATGATTCTTGTCTTATTCTTTGGGATGCCCGGAGTGGTACAGGCCCAGCTGTTAAGGTTGAGAAAGCTCATGGTGGTGATGTTCATTGTGTTGACTGGAACCTCCATGATGTTAACTATATCTTAACTGGTTCTGCGGATAATTCTGTCCGTATGTGGGACCGGAGAAATCTGGGTTCTGGAGGAGCTGGCATTCCAGTTCACAAATTTGAGGGTCATAAAGCTGCTGTTCTTTGTGTTCAGTGGTCACCTGACAAGGCATCTGTATTTGGAAGCTCTGCGGAAGACGGCTTCTTAAATGTGTGGGATCATGAGAAGGTTGGAAATAAGAAAAATCCCAACGCACCTGCTGGGCTTTTCTTTCAACATGCTGGTCATAGGGATAAGATTGTAGACTTCCACTGGAATTCTTCGGATCCTTGGACTATTGTGAGTGTGTCTGATGATGGTGAGAGTACTGGTGGAGGTGGAACATTGCAGATATGGCGCATGAGTGACCTGATATACCGTCCAGAAGATGAAGTTCTTGCAGAGCTGGAGAACTTCAAGACTCACTTGGCCAGCTGTGCTCCAAGGAGCTGA
Protein Sequence
  • >LOC_Os01g51300.1
    MKEKGSRAAVDERYAQWKSLIPVLYDWFANHNLVWPSLSCRWGPQFEKATYKNRQRLYLSEQTDGTVPNTLVIANCEVVKPRVAAAEHISQFNEEARSPFVKKYKTIIHPGEVNRIRELPQNSKIIATHTDSPDVLIWDVEAQPNRQAQLAQMESRPDLVPPDSRPDLILRGHKDIAEFALAMCPAEPYVLSGGKDKSVVWWSIQDHISALGDSSKTESSPGASGSKGKTANDKDSPKVDPRGIFLGHDSTVEDVQFCPSSAQEFCSVGDDSCLILWDARSGTGPAVKVEKAHGGDVHCVDWNLHDVNYILTGSADNSVRMWDRRNLGSGGAGIPVHKFEGHKAAVLCVQWSPDKASVFGSSAEDGFLNVWDHEKVGNKKNPNAPAGLFFQHAGHRDKIVDFHWNSSDPWTIVSVSDDGESTGGGGTLQIWRMSDLIYRPEDEVLAELENFKTHLASCAPRS*
  • >LOC_Os01g51300.2
    MKEKGSRAAVDERYAQWKSLIPVLYDWFANHNLVWPSLSCRWGPQFEKATYKNRQRLYLSEQTDGTVPNTLVIANCEVVKPRVAAAEHISQFNEEARSPFVKKYKTIIHPGEVNRIRELPQNSKIIATHTDSPDVLIWDVEAQPNRQAQLAQMESRPDLVPPDSRPDLILRGHKDIAEFALAMCPAEPYVLSGGKDKSVVWWSIQDHISALGDSSKTESSPGASGSKGKTANDKDSPKVDPRGIFLGHDSTVEDVQFCPSSAQEFCSVGDDSCLILWDARSGTGPAVKVEKAHGGDVHCVDWNLHDVNYILTGSADNSVRMWDRRNLGSGGAGIPVHKFEGHKAAVLCVQWSPDKASVFGSSAEDGFLNVWDHEKVGNKKNPNAPAGLFFQHAGHRDKIVDFHWNSSDPWTIVSVSDDGESTGGGGTLQIWRMSDLIYRPEDEVLAELENFKTHLASCAPRS*