Information report for SPL28
Gene Details
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Functional Descriptions
- The SPL28 mutant also exhibited significantly enhanced resistance to rice blast and bacterial blight.
- SPL28 appears to be involved in the regulation of vesicular trafficking, and SPL28 dysfunction causes the formation of hypersensitive response (HR)-like lesions, leading to the initiation of leaf senescence.
- SPL28 encodes a clathrin-associated adaptor protein complex 1, medium subunit micro 1 (AP1M1) and is responsible for spotted leaf and early senescence in rice (Oryza sativa).
- However, after flowering, SPL28 mutants exhibited a significant decrease in chlorophyll content, soluble protein content, and photosystem II efficiency, and high concentrations of reactive oxygen species (ROS), phytoalexin, callose, and autofluorescent phenolic compounds that localized in or around the lesions.
- To expand our understanding of cell death in plant defense responses, we isolated a novel rice (Oryza sativa) spotted leaf mutant (SPL28) that displays a lesion mimic phenotype in the absence of pathogen attack through treatment of Hwacheongbyeo (an elite Korean japonica cultivar) with N-methyl-N-nitrosourea (MNU).
Functional Keywords
- blight , bacterial-blight , blast , senescence , flower , defense-response , cell-death , defense , leaf
Literature and News
Gene Resources
- UniProt: Q0JK13
- EMBL: AP014957
- AlphaFoldDB: Q0JK13
- EnsemblPlants: Os01t0703600-01
- Gramene: Os01t0703600-01
- KEGG: osa:4324247
- Orthologous matrix: KPLIWCD
- InterPro: IPR001392, IPR011012, IPR018240
Homologs
- Actinidia chinensis: Achn041401
- Daucus carota: DCAR_026528
- Manihot esculenta: Manes.09G178800
- Prunus avium: Pav_sc0006018.1_g500.1.mk
- Solanum melongena: Sme2.5_00544.1_g00012.1
Sequences
cDNA Sequence
- >LOC_Os01g50770.1
ACCGTCTCCACTCCCGATCTCCCCGCTCCCCGCGTCCCGTCTCCGATCCACCCAAACACCCGCACCGCCGCGCTACGCGAGCACGGCACCGCGGCGCGGCGCGACTCCGCAGGCGACAAGCCGAGGAGAATCCCGCGCCCGCCCGCCCGCATCACCGCCGGATGGCCGCCGGCCGTCGCCGGTAGGGCGATAGGCGGGTAGATCCAGATCCGGCGGGGCGGGATCATATACACCCCGCGCCGATGGCGGGCGCGGTGTCGGCGCTGTTCCTTCTGGACATCAAGGGCCGCGTCCTCGTCTGGCGCGACTACCGCGGCGACGTCTCCGCCCTCCAGGCTGAGCGCTTCTTCACCAAGCTCCTCGACAAGGAGGGCGACTCGGAGGCGCACTCGCCGGTGGTCTACGACGATGCCGGGGTCACCTACATGTTCATCCAGCACAACAACGTGTTCCTACTAACCGCCTCCCGCCAGAACTGCAACGCCGCCAGCATCCTCCTCTTCCTCCACCGCGTCGTTGATGTGTTCAAGCACTATTTCGAAGAGTTGGAGGAAGAGTCGCTGAGGGATAACTTTGTCGTTGTGTATGAGTTGCTTGATGAAATGATGGATTTTGGGTACCCACAATACACGGAGGCGAAAATCTTAAGTGAATTCATTAAGACGGATGCGTACAGGATGGAGGTATCACAGAGGCCACCTATGGCAGTGACAAATGCCGTGTCATGGCGGAGTGAGGGTATTCGGTACAAGAAGAATGAAGTGTTCTTGGATGTTGTGGAGAGTGTTAACATTCTTGTTAACAGCAATGGGCAGATTGTGAGATCAGATGTTGTTGGGGCACTGAAGATGCGGACATATTTGAGTGGAATGCCTGAGTGCAAACTTGGATTGAACGATAGGGTTCTTTTGGAGGCTCAAGGCCGAGCTACTAAAGGAAAAGCAATAGATCTTGATGATATTAAATTTCACCAGTGCGTGAGGTTGGCTAGATTTGAGAATGATAGAACTATATCCTTCATACCTCCTGACGGATCTTTCGATCTAATGACATACAGACTTAGCACCCAGGTAAAACCACTTATCTGGGTGGAAGCACAAATTGAGAAACATTCAAGAAGCCGAATAGAACTTATGGTGAAAGCAAGAAGTCAGTTTAAGGAAAGGAGCACAGCAACAAATGTTGAAATTGAAGTGCCTGTTCCTTCAGATGCTACGAACCCTAATATAAGAACTTCAATGGGCTCTGCTGCATATGCACCTGAGAGAGATGCAATGGTCTGGAAAGTAAAATCATTTCCTGGTGGCAAGGATTACATGTGCAGAGCTGAATTTAGTCTTCCAAGTATAACTGCAGAAGAAGCAGCTCCTGAAAAGAAGGCACCAATACGAGTGAAATTTGAGATACCATATTTCACTGTGTCGGGTATTCAGGTTCGCTATCTGAAGATCATTGAAAAGAGTGGGTACCAGGCGCTTCCTTGGGTTAGATACATTACAATGGCTGGTGAATACGAACTGAGACTTATATGAGTTATCTCTGATATGGCTGCTCAAAGATTTTGATGCTAAATTTCAACTCCCAAGATACATGTTTGATGCTCGATATACAACATATAGTTGGCATTGGGACGTTGCCATGGAAGTCTTTGAAAAGCTCAACCATGCAAGGTTTTTCTGTCTCGTTGTTCATCTTGTTAGTGTTACTTTACCATGGTTACTGTAAGTTACCCATCAAGTATAGCTTTGAAAAAAACTTTTTTTTTGTTCACTTGTATCATTATTGATTTATTTTTGTGGGTGTCGAACTTTCCCCCTTTTGTACTACACAAAAATCTTGAGCTGAATTGGTATAAATGAAATACCTGGGCGGTGCTTTTGAAGTCCAGATTAAAACTGCTGAACCTGTAGAACTATTTTTAAGGCATGCTTTCTGTTGTGT - >LOC_Os01g50770.2
ACCGTCTCCACTCCCGATCTCCCCGCTCCCCGCGTCCCGTCTCCGATCCACCCAAACACCCGCACCGCCGCGCTACGCGAGCACGGCACCGCGGCGCGGCGCGACTCCGCAGGCGACAAGCCGAGGAGAATCCCGCGCCCGCCCGCCCGCATCACCGCCGGATGGCCGCCGGCCGTCGCCGGTAGGGCGATAGGCGGGTAGATCCAGATCCGGCGGGGCGGGATCATATACACCCCGCGCCGATGGCGGGCGCGGTGTCGGCGCTGTTCCTTCTGGACATCAAGGGCCGCGTCCTCGTCTGGCGCGACTACCGCGGCGACGTCTCCGCCCTCCAGGCTGAGCGCTTCTTCACCAAGCTCCTCGACAAGGAGGGCGACTCGGAGGCGCACTCGCCGGTGGTCTACGACGATGCCGGGGTCACCTACATGTTCATCCAGCACAACAACGTGTTCCTACTAACCGCCTCCCGCCAGAACTGCAACGCCGCCAGCATCCTCCTCTTCCTCCACCGCGTCGTTGATGTGTTCAAGCACTATTTCGAAGAGTTGGAGGAAGAGTCGCTGAGGGATAACTTTGTCGTTGTGTATGAGTTGCTTGATGAAATGATGGATTTTGGGTACCCACAATACACGGAGGCGAAAATCTTAAGTGAATTCATTAAGACGGATGCGTACAGGATGGAGGTATCACAGAGGCCACCTATGGCAGTGACAAATGCCGTGTCATGGCGGAGTGAGGGTATTCGGTACAAGAAGAATGAAGTGTTCTTGGATGTTGTGGAGAGTGTTAACATTCTTGTTAACAGCAATGGGCAGATTGTGAGATCAGATGTTGTTGGGGCACTGAAGATGCGGACATATTTGAGTGGAATGCCTGAGTGCAAACTTGGATTGAACGATAGGGTTCTTTTGGAGGCTCAAGGCCGAGCTACTAAAGGAAAAGCAATAGATCTTGATGATATTAAATTTCACCAGTGCGTGAGGTTGGCTAGATTTGAGAATGATAGAACTATATCCTTCATACCTCCTGACGGATCTTTCGATCTAATGACATACAGACTTAGCACCCAGGTAAAACCACTTATCTGGGTGGAAGCACAAATTGAGAAACATTCAAGAAGCCGAATAGAACTTATGGTGAAAGCAAGAAGTCAGTTTAAGGAAAGGAGCACAGCAACAAATGTTGAAATTGAAGTGCCTGTTCCTTCAGATGCTACGAACCCTAATATAAGAACTTCAATGGGCTCTGCTGCATATGCACCTGAGAGAGATGCAATGGTCTGGAAAGTAAAATCATTTCCTGGTGGCAAGGATTACATGTGCAGAGCTGAATTTAGTCTTCCAAGTATAACTGCAGAAGAAGCAGCTCCTGAAAAGAAGGCACCAATACGAGTGAAATTTGAGATACCATATTTCACTGTGTCGGGTATTCAGGTTCGCTATCTGAAGATCATTGAAAAGAGTGGGTACCAGGCGCTTCCTTGGGTTAGATACATTACAATGGCTGGTGAATACGAACTGAGACTTATATGAGTTATCTCTGATATGGCTGCTCAAAGATTTTGATGCTAAATTTCAACTCCCAAGATACATGTTTGATGCTCGATATACAACATATAGTTGGCATTGGGACGTTGCCATGGAAGTCTTTGAAAAGCTCAACCATGCAAGGTTTTTCTGTCTCGTTGTTCATCTTGTTAGTGTTACTTTACCATGGTTACTGTAAGTTACCCATCAAGTATAGCTTTGAAAAAAACTTTTTTTTTGTTCACTTGTATCATTATTGATTTATTTTTGTGGGTGTCGAACTTTCCCCCTTTTGTACTACACAAAAATCTTGAGCTGAATTGGTATAAATGAAATACCTGGGCGGTGCTTTTGAAGTCCAGATTAAAACTGCTGAACCTGTAGAACTATTTTTAAGGCATGCTTTCTGTTGTGT
CDS Sequence
- >LOC_Os01g50770.1
ATGGCGGGCGCGGTGTCGGCGCTGTTCCTTCTGGACATCAAGGGCCGCGTCCTCGTCTGGCGCGACTACCGCGGCGACGTCTCCGCCCTCCAGGCTGAGCGCTTCTTCACCAAGCTCCTCGACAAGGAGGGCGACTCGGAGGCGCACTCGCCGGTGGTCTACGACGATGCCGGGGTCACCTACATGTTCATCCAGCACAACAACGTGTTCCTACTAACCGCCTCCCGCCAGAACTGCAACGCCGCCAGCATCCTCCTCTTCCTCCACCGCGTCGTTGATGTGTTCAAGCACTATTTCGAAGAGTTGGAGGAAGAGTCGCTGAGGGATAACTTTGTCGTTGTGTATGAGTTGCTTGATGAAATGATGGATTTTGGGTACCCACAATACACGGAGGCGAAAATCTTAAGTGAATTCATTAAGACGGATGCGTACAGGATGGAGGTATCACAGAGGCCACCTATGGCAGTGACAAATGCCGTGTCATGGCGGAGTGAGGGTATTCGGTACAAGAAGAATGAAGTGTTCTTGGATGTTGTGGAGAGTGTTAACATTCTTGTTAACAGCAATGGGCAGATTGTGAGATCAGATGTTGTTGGGGCACTGAAGATGCGGACATATTTGAGTGGAATGCCTGAGTGCAAACTTGGATTGAACGATAGGGTTCTTTTGGAGGCTCAAGGCCGAGCTACTAAAGGAAAAGCAATAGATCTTGATGATATTAAATTTCACCAGTGCGTGAGGTTGGCTAGATTTGAGAATGATAGAACTATATCCTTCATACCTCCTGACGGATCTTTCGATCTAATGACATACAGACTTAGCACCCAGGTAAAACCACTTATCTGGGTGGAAGCACAAATTGAGAAACATTCAAGAAGCCGAATAGAACTTATGGTGAAAGCAAGAAGTCAGTTTAAGGAAAGGAGCACAGCAACAAATGTTGAAATTGAAGTGCCTGTTCCTTCAGATGCTACGAACCCTAATATAAGAACTTCAATGGGCTCTGCTGCATATGCACCTGAGAGAGATGCAATGGTCTGGAAAGTAAAATCATTTCCTGGTGGCAAGGATTACATGTGCAGAGCTGAATTTAGTCTTCCAAGTATAACTGCAGAAGAAGCAGCTCCTGAAAAGAAGGCACCAATACGAGTGAAATTTGAGATACCATATTTCACTGTGTCGGGTATTCAGGTTCGCTATCTGAAGATCATTGAAAAGAGTGGGTACCAGGCGCTTCCTTGGGTTAGATACATTACAATGGCTGGTGAATACGAACTGAGACTTATATGA - >LOC_Os01g50770.2
ATGGCGGGCGCGGTGTCGGCGCTGTTCCTTCTGGACATCAAGGGCCGCGTCCTCGTCTGGCGCGACTACCGCGGCGACGTCTCCGCCCTCCAGGCTGAGCGCTTCTTCACCAAGCTCCTCGACAAGGAGGGCGACTCGGAGGCGCACTCGCCGGTGGTCTACGACGATGCCGGGGTCACCTACATGTTCATCCAGCACAACAACGTGTTCCTACTAACCGCCTCCCGCCAGAACTGCAACGCCGCCAGCATCCTCCTCTTCCTCCACCGCGTCGTTGATGTGTTCAAGCACTATTTCGAAGAGTTGGAGGAAGAGTCGCTGAGGGATAACTTTGTCGTTGTGTATGAGTTGCTTGATGAAATGATGGATTTTGGGTACCCACAATACACGGAGGCGAAAATCTTAAGTGAATTCATTAAGACGGATGCGTACAGGATGGAGGTATCACAGAGGCCACCTATGGCAGTGACAAATGCCGTGTCATGGCGGAGTGAGGGTATTCGGTACAAGAAGAATGAAGTGTTCTTGGATGTTGTGGAGAGTGTTAACATTCTTGTTAACAGCAATGGGCAGATTGTGAGATCAGATGTTGTTGGGGCACTGAAGATGCGGACATATTTGAGTGGAATGCCTGAGTGCAAACTTGGATTGAACGATAGGGTTCTTTTGGAGGCTCAAGGCCGAGCTACTAAAGGAAAAGCAATAGATCTTGATGATATTAAATTTCACCAGTGCGTGAGGTTGGCTAGATTTGAGAATGATAGAACTATATCCTTCATACCTCCTGACGGATCTTTCGATCTAATGACATACAGACTTAGCACCCAGGTAAAACCACTTATCTGGGTGGAAGCACAAATTGAGAAACATTCAAGAAGCCGAATAGAACTTATGGTGAAAGCAAGAAGTCAGTTTAAGGAAAGGAGCACAGCAACAAATGTTGAAATTGAAGTGCCTGTTCCTTCAGATGCTACGAACCCTAATATAAGAACTTCAATGGGCTCTGCTGCATATGCACCTGAGAGAGATGCAATGGTCTGGAAAGTAAAATCATTTCCTGGTGGCAAGGATTACATGTGCAGAGCTGAATTTAGTCTTCCAAGTATAACTGCAGAAGAAGCAGCTCCTGAAAAGAAGGCACCAATACGAGTGAAATTTGAGATACCATATTTCACTGTGTCGGGTATTCAGGTTCGCTATCTGAAGATCATTGAAAAGAGTGGGTACCAGGCGCTTCCTTGGGTTAGATACATTACAATGGCTGGTGAATACGAACTGAGACTTATATGA
Protein Sequence
- >LOC_Os01g50770.1
MAGAVSALFLLDIKGRVLVWRDYRGDVSALQAERFFTKLLDKEGDSEAHSPVVYDDAGVTYMFIQHNNVFLLTASRQNCNAASILLFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEMMDFGYPQYTEAKILSEFIKTDAYRMEVSQRPPMAVTNAVSWRSEGIRYKKNEVFLDVVESVNILVNSNGQIVRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRATKGKAIDLDDIKFHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQIEKHSRSRIELMVKARSQFKERSTATNVEIEVPVPSDATNPNIRTSMGSAAYAPERDAMVWKVKSFPGGKDYMCRAEFSLPSITAEEAAPEKKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRYITMAGEYELRLI* - >LOC_Os01g50770.2
MAGAVSALFLLDIKGRVLVWRDYRGDVSALQAERFFTKLLDKEGDSEAHSPVVYDDAGVTYMFIQHNNVFLLTASRQNCNAASILLFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEMMDFGYPQYTEAKILSEFIKTDAYRMEVSQRPPMAVTNAVSWRSEGIRYKKNEVFLDVVESVNILVNSNGQIVRSDVVGALKMRTYLSGMPECKLGLNDRVLLEAQGRATKGKAIDLDDIKFHQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAQIEKHSRSRIELMVKARSQFKERSTATNVEIEVPVPSDATNPNIRTSMGSAAYAPERDAMVWKVKSFPGGKDYMCRAEFSLPSITAEEAAPEKKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRYITMAGEYELRLI*