Information report for OsMKKK70
Gene Details
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Functional Descriptions
- OsMKKK70 regulates grain size and leaf angle in rice through the OsMKK4-OsMAPK6-OsWRKY53 signaling pathway.
- Here we demonstrate that the Oryza sativa Mitogen Activated Protein Kinase Kinase Kinase OsMKKK70 regulates grain size and leaf angle in rice.
- The osmkkk62/70 double mutant and the osmkkk55/62/70 triple mutant displayed significantly smaller seeds and a more erect leaf angle compared to the wild type, indicating that OsMKKK70 functions redundantly with its homologs OsMKKK62 and OsMKKK55.
- Taken together, these findings suggest that OsMKKK70 might regulates grain size and leaf angle in rice by activating OsMAPK6 and that OsMKKK70, OsMKK4, OsMAPK6, and OsWRKY53 function in a common signaling pathway that controls grain shape and leaf angle.
- Biochemical analysis demonstrated that OsMKKK70 is an active kinase and that OsMKKK70 interacts with OsMKK4 and promotes OsMAPK6 phosphorylation.
- In this study, a function of OsMKKK70 (Mitogen Activated Protein Kinase Kinase Kinase 70) in response to CTB was characterized.
- OsMKKK70 overexpression (OsMKKK70-OE) plants were more sensitive to cold stress at the booting stage with a lower seed setting and pollen fertility, but there was no significant difference between the OsMKKK70 mutant and WT.
- Considering the effect of functional redundancy, we further tested the CTB response of osmkkk62/70 and osmkkk55/62/70, the double and triple mutants of OsMKKK70 with its closest homologs OsMKKK62 and OsMKKK55, and found that osmkkk62/70 and osmkkk55/62/70 displayed significantly increased CTB with a higher seed setting and pollen fertility, indicating that OsMKKK70 negatively regulates rice CTB.
- OsMKKK70 expression was rapidly induced by cold stress at the booting stage.
- OsMKKK70 Negatively Regulates Cold Tolerance at Booting Stage in Rice.
- Together, these findings suggest that OsMKKK70 negatively regulates rice CTB by fine-tuning GA levels in anthers.
Functional Keywords
- leaf , grain , grain-size , Kinase , protein-kinase , erect , kinase , leaf-angle , grain-shape , pollen , stress , seed , tolerance , cold-tolerance , cold-stress , cold , ga , GA , Pollen-Fertility , seed-set
Literature and News
Homologs
- Panicum virgatum: Pavir.5NG449400, Pavir.5KG498100
- Setaria italica: Seita.5G284400
- Setaria viridis: SEVIR_5G287100v2
Sequences
cDNA Sequence
- >LOC_Os01g50410.1
ATGGCTAAGCAGCTCAGGCGGGTGCGCACGCTGGGCCGCGGGGCGTCCGGCGCCGTGGTGTGGCTCGCGTCCGACGACGACTCAGGGGAGCTCATGGCCGTCAAGTCGGCCTCCGCCGGCGGCGCCGCGGCGCAGCTGCGGCGAGAGGGGCGTGTCCTGTCCGGGCTCTGCTCGCCGCACATCGTCCCCTGCCTCGGATCGCGCGCCGCCGCGGGCGGCGAGTACCAGCTGTTCCTCGAGTTCGCGCCCGGCGGGTCGCTCGCCGACGAGGCCGCCAGGAACGGGGGCTGCCTCCCGGAGCCGGCCATCCGGGCGTACGCCGCTGACGTGGCGAGGGGGCTGGCGTACCTCCACGGGAATTCGCTGGTGCACGGCGACGTCAAGGCGAGGAACGTCGTGATCGGGAGCGACGGCCGGGCGAGGCTCACGGACTTCGGGTGCGCGAGGGTCATGGACTCGGCGGGGCCGATCGGCGGCACGCCGGCGTTCATGGCGCCGGAGGTGGCGCGCGGGGAGGAGCAGGGGCCGGCGGCCGATGTCTGGGCCCTCGGCTGCACCATCATCGAGATGGCCACCGGCCGCGCGCCGTGGAGCGACATGGACGACATCCTTGCCGCAGTCCACCGGATCGGGTACACGAACGCCGTGCCGGAGGTTCCCGGGTGGCTGTCAGCGGAGGCCAAGGACTTCCTGGATGGCTGCTTCGAGAGGAACGCGTCCGACAGGTCAACGGCGGCGCAGCTCCTGGAACACCCATTCGTTGCCTCCGCCGCGGCCCTCGACCGCTGGCCGGAGCCGGCGAAGCAAGAACGTGCATCCCCCAAGAGCACGCTGCATGACGCGTTCTGGGACTCAGACACCGACGACGAGGACGACGAGATGCCAACCGGCGCGGCGGAGAGGATCGGTGCATTGGCGTGCGCCGCCTCGGCCTTGCCGGACTGGGACTCCGACGAAGGATGGATCGAGGTGCACGACGAGGTCTCCTTCGCCGCCGTCACGCCGCCGGCCAGCGACGCGGACTACTTCGTCTGGGCAGAACTGTCTGACCCAGAGATGGAGCAGTTCGCCGTCGCCGCGGATGGCGTCAACCATGTCCCGCGCAATGAAGCAGAAGCGATCGAATCCTCCATTAGGCAGGGCAGTTACCTGCACGTACATCTTGGCAGTGGTAAAAATGAAATTTTTCACCCGTTCGATACTGACGGGACTGAATCAGGGCTTGATGCCCACCGGCTCACAGCATTGCAAAAACACCAGTGGAATCTGAGCTCAAGATTGCCACATTCGAGCTGGATTGATCGAACCTTTGGAAGTGATCTAGATATTGGTGTTCAATTGGCAACCACATGCTGA
CDS Sequence
- >LOC_Os01g50410.1
ATGGCTAAGCAGCTCAGGCGGGTGCGCACGCTGGGCCGCGGGGCGTCCGGCGCCGTGGTGTGGCTCGCGTCCGACGACGACTCAGGGGAGCTCATGGCCGTCAAGTCGGCCTCCGCCGGCGGCGCCGCGGCGCAGCTGCGGCGAGAGGGGCGTGTCCTGTCCGGGCTCTGCTCGCCGCACATCGTCCCCTGCCTCGGATCGCGCGCCGCCGCGGGCGGCGAGTACCAGCTGTTCCTCGAGTTCGCGCCCGGCGGGTCGCTCGCCGACGAGGCCGCCAGGAACGGGGGCTGCCTCCCGGAGCCGGCCATCCGGGCGTACGCCGCTGACGTGGCGAGGGGGCTGGCGTACCTCCACGGGAATTCGCTGGTGCACGGCGACGTCAAGGCGAGGAACGTCGTGATCGGGAGCGACGGCCGGGCGAGGCTCACGGACTTCGGGTGCGCGAGGGTCATGGACTCGGCGGGGCCGATCGGCGGCACGCCGGCGTTCATGGCGCCGGAGGTGGCGCGCGGGGAGGAGCAGGGGCCGGCGGCCGATGTCTGGGCCCTCGGCTGCACCATCATCGAGATGGCCACCGGCCGCGCGCCGTGGAGCGACATGGACGACATCCTTGCCGCAGTCCACCGGATCGGGTACACGAACGCCGTGCCGGAGGTTCCCGGGTGGCTGTCAGCGGAGGCCAAGGACTTCCTGGATGGCTGCTTCGAGAGGAACGCGTCCGACAGGTCAACGGCGGCGCAGCTCCTGGAACACCCATTCGTTGCCTCCGCCGCGGCCCTCGACCGCTGGCCGGAGCCGGCGAAGCAAGAACGTGCATCCCCCAAGAGCACGCTGCATGACGCGTTCTGGGACTCAGACACCGACGACGAGGACGACGAGATGCCAACCGGCGCGGCGGAGAGGATCGGTGCATTGGCGTGCGCCGCCTCGGCCTTGCCGGACTGGGACTCCGACGAAGGATGGATCGAGGTGCACGACGAGGTCTCCTTCGCCGCCGTCACGCCGCCGGCCAGCGACGCGGACTACTTCGTCTGGGCAGAACTGTCTGACCCAGAGATGGAGCAGTTCGCCGTCGCCGCGGATGGCGTCAACCATGTCCCGCGCAATGAAGCAGAAGCGATCGAATCCTCCATTAGGCAGGGCAGTTACCTGCACGTACATCTTGGCAGTGGTAAAAATGAAATTTTTCACCCGTTCGATACTGACGGGACTGAATCAGGGCTTGATGCCCACCGGCTCACAGCATTGCAAAAACACCAGTGGAATCTGAGCTCAAGATTGCCACATTCGAGCTGGATTGATCGAACCTTTGGAAGTGATCTAGATATTGGTGTTCAATTGGCAACCACATGCTGA
Protein Sequence
- >LOC_Os01g50410.1
MAKQLRRVRTLGRGASGAVVWLASDDDSGELMAVKSASAGGAAAQLRREGRVLSGLCSPHIVPCLGSRAAAGGEYQLFLEFAPGGSLADEAARNGGCLPEPAIRAYAADVARGLAYLHGNSLVHGDVKARNVVIGSDGRARLTDFGCARVMDSAGPIGGTPAFMAPEVARGEEQGPAADVWALGCTIIEMATGRAPWSDMDDILAAVHRIGYTNAVPEVPGWLSAEAKDFLDGCFERNASDRSTAAQLLEHPFVASAAALDRWPEPAKQERASPKSTLHDAFWDSDTDDEDDEMPTGAAERIGALACAASALPDWDSDEGWIEVHDEVSFAAVTPPASDADYFVWAELSDPEMEQFAVAADGVNHVPRNEAEAIESSIRQGSYLHVHLGSGKNEIFHPFDTDGTESGLDAHRLTALQKHQWNLSSRLPHSSWIDRTFGSDLDIGVQLATTC*