Information report for OsYUCCA1;OsYUC1
Gene Details
|
|
Functional Descriptions
- Furthermore, exogenous application of IAA or overexpression of the auxin biosynthesis gene OsYUCCA1 phenocopies the dao mutants.
- To investigate the molecular mechanisms of IAA synthesis in rice (Oryza sativa), we identified seven YUCCA-like genes (named OsYUCCA1-7) in the rice genome.
- Plants overexpressing OsYUCCA1 exhibited increased IAA levels and characteristic auxin overproduction phenotypes, whereas plants expressing antisense OsYUCCA1 cDNA displayed defects that are similar to those of rice auxin-insensitive mutants.
- These observations are consistent with an important role for the rice enzyme OsYUCCA1 in IAA biosynthesis via the tryptophan-dependent pathway.
- OsYUCCA1 was expressed in almost all of the organs tested, but its expression was restricted to discrete areas, including the tips of leaves, roots, and vascular tissues, where it overlapped with expression of a beta-glucuronidase reporter gene controlled by the auxin-responsive DR5 promoter.
- Trp feeding completely rescued the mutant phenotypes and moderate expression of OsYUCCA1, which encodes a key enzyme in Trp-dependent IAA biosynthesis, also rescued plant height and root length, indicating that the abnormal phenotypes of tdd1 are caused predominantly by Trp and IAA deficiency.
Literature and News
- A role for a dioxygenase in auxin metabolism and reproductive development in rice . DOI: 10.1016/j.devcel.2013.09.005 ; PMID: 24094741
- Cloning, characterization and expression of OsFMO(t) in rice encoding a flavin monooxygenase . DOI: 10.1007/s12041-013-0297-0 ; PMID: 24371168
- A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos . DOI: 10.1111/j.1365-313X.2009.03952.x ; PMID: 19682283
- Auxin biosynthesis by the YUCCA genes in rice . DOI: 10.1104/pp.106.091561 ; PMID: 17220367
- Calcium lignosulfonate improves proliferation of recalcitrant indica rice callus via modulation of auxin biosynthesis and enhancement of nutrient absorption . DOI: 10.1016/j.plaphy.2021.01.046 ; PMID: 33581621
- Targeted Phytohormone Profiling Identifies Potential Regulators of Spikelet Sterility in Rice under Combined Drought and Heat Stress . DOI: 10.3390/ijms222111690 ; PMID: 34769121
Gene Resources
- NCBI ID: AK105488
Sequences
cDNA Sequence
- >LOC_Os01g45760.2
ACCTAGTTGGCAGTAAAAAAAGCGCCGCTACCTACACACACAACCGCTCTGCACAAGGGGCCATGGACAACAAGCCGGCGCAAGAACGCCGCGAGACGTGGGTCCCGGGCGCCGTCATCGTCGGCGCGGGCCCGTCGGGGCTCGCCGCGGCGGCGTGCCTCGCGGCGCGGGGCGTTCCGGCGACGGTGCTTGAGCGGTCCGACTCGCTGGCGTCCACGTGGCGCCACCGCATGTACGACCGCCTCGCTCTCCACCTGCCCAAGCGCTTCTGCGAGCTGCCGCTCCTCCCGTTCCCGGAGGAGTACCCGACGTACCCGTCCAAGGACCAGTTCGTGGCGTACATGGAGGCGTACGCCGCGGCGGCGGGCGTCGCGCCGCGCTTCGGCGCCACCGTCGAGGAGGCCGCGTTCGACGCGGCCGTCGGCGCATGGAGGGTGCGTCTCGACGGCGGGGAGGTGCTCATGGCGCGGTGGCTCGTCGTCGCGACGGGGGAGAACGCCGAGCCGCGGGTGCCGGACTTTCCCGGCATGCAGAAGTTCGCCGGGTGTGCCATGCATACTTCCGAGTACAAGTCCGGCGAGCAGTTTGCCGGTAAGAAGGTGTTGGTCGTGGGATGCGGGAATTCTGGCATGGAAGTGAGCTTGGATTTGTGCCGGCATGGCGCGAAGCCCTCCATGGTGGTGCGCAACACGGTGCATGTCCTACCAAGGGAAATGTTCGGTCTATCCACGTTCGGCATCGCCATGGCGCTGCTGCGATGGCTACCGGTTCAGCTCGTCGACCGGTTCCTTCTCACGGCGGCTCACCTCATCCTCGGTAACACGGGGCAGTTCGGCCTAAGGCGGCCCAAAACAGGGCCCATCGAGCTCAAGAACCTCACCGGCAGGACACCCGTACTGGACGTTGGGACGCTAGACCACATCAAATCCGGCAAAATTAAGGTAGTGGGAGCAGTGAAGGAGATGACGAGGCAAGGGGTCAGGTTCACGGATGGCAAGGAGGAGCAATTCGACACAATAATCCTCGCCACAGGCTACAGGAGCAACGTGCCATCTTGGCTCAAGGTAAAGCAGAGTGTGACTCACTCTTGTTCGTTTTATTTTTCTTTCTGTCCGCACTGCACTATACTACTACTAGTGATATATATCTCGAGATGATACGGATTATATGGTCAACTGTCAAGCCCAAGGATGTTAATTATATGTCTATATGTACGTGTGCTTTATGTGTTGTAGGATGCCGGTGACTTGTTCACGAGGGAGGGCATTTCCAAGGTCCCCTTCCCTAACAGCTGGAGAGGGCGGAATGGTCTCTACACCGTCGGATTCACTCAGCGGGGGCTCCTCGGCACGTCATCGGACGCCCTCAACGTCGCCAAAGACATACATTGCCAATGGAGAGAAAGGGACCGATCTGCTATTAATGTACTTGAAATAAGCAACTCTTCCTTTTGATTTGACTGGAAATGTACAGTGTTTTGATTAGGAATATTGTATTGTGTGCATATAACACCATGTTCTATCTCCCTTTTCAACAAAGACTGATAATCTTGCTCTGCCTTATGTCGATTACTTGCCTATTGAGCATCTTTTGGGCAAACTGTTATTATGACAAGAATTTAGAATTTATATCAATACTCCTTTAAA - >LOC_Os01g45760.1
ACCTAGTTGGCAGTAAAAAAAGCGCCGCTACCTACACACACAACCGCTCTGCACAAGGGGCCATGGACAACAAGCCGGCGCAAGAACGCCGCGAGACGTGGGTCCCGGGCGCCGTCATCGTCGGCGCGGGCCCGTCGGGGCTCGCCGCGGCGGCGTGCCTCGCGGCGCGGGGCGTTCCGGCGACGGTGCTTGAGCGGTCCGACTCGCTGGCGTCCACGTGGCGCCACCGCATGTACGACCGCCTCGCTCTCCACCTGCCCAAGCGCTTCTGCGAGCTGCCGCTCCTCCCGTTCCCGGAGGAGTACCCGACGTACCCGTCCAAGGACCAGTTCGTGGCGTACATGGAGGCGTACGCCGCGGCGGCGGGCGTCGCGCCGCGCTTCGGCGCCACCGTCGAGGAGGCCGCGTTCGACGCGGCCGTCGGCGCATGGAGGGTGCGTCTCGACGGCGGGGAGGTGCTCATGGCGCGGTGGCTCGTCGTCGCGACGGGGGAGAACGCCGAGCCGCGGGTGCCGGACTTTCCCGGCATGCAGAAGTTCGCCGGGTGTGCCATGCATACTTCCGAGTACAAGTCCGGCGAGCAGTTTGCCGGTAAGAAGGTGTTGGTCGTGGGATGCGGGAATTCTGGCATGGAAGTGAGCTTGGATTTGTGCCGGCATGGCGCGAAGCCCTCCATGGTGGTGCGCAACACGGTGCATGTCCTACCAAGGGAAATGTTCGGTCTATCCACGTTCGGCATCGCCATGGCGCTGCTGCGATGGCTACCGGTTCAGCTCGTCGACCGGTTCCTTCTCACGGCGGCTCACCTCATCCTCGGTAACACGGGGCAGTTCGGCCTAAGGCGGCCCAAAACAGGGCCCATCGAGCTCAAGAACCTCACCGGCAGGACACCCGTACTGGACGTTGGGACGCTAGACCACATCAAATCCGGCAAAATTAAGGTAGTGGGAGCAGTGAAGGAGATGACGAGGCAAGGGGTCAGGTTCACGGATGGCAAGGAGGAGCAATTCGACACAATAATCCTCGCCACAGGCTACAGGAGCAACGTGCCATCTTGGCTCAAGGTAAAGCAGAGTGTGACTCACTCTTGTTCGTTTTATTTTTCTTTCTGTCCGCACTGCACTATACTACTACTAGTGATATATATCTCGAGATGATACGGATTATATGGTCAACTGTCAAGCCCAAGGATGTTAATTATATGTCTATATGTACGTGTGCTTTATGTGTTGTAGGATGCCGGTGACTTGTTCACGAGGGAGGGCATTTCCAAGGTCCCCTTCCCTAACAGCTGGAGAGGGCGGAATGGTCTCTACACCGTCGGATTCACTCAGCGGGGGCTCCTCGGCACGTCATCGGACGCCCTCAACGTCGCCAAAGACATACATTGCCAATGGAGAGAAAGGGACCGATCTGCTATTAATGTACTTGAAATAAGCAACTCTTCCTTTTGATTTGACTGGAAATGTACAGTGTTTTGATTAGGAATATTGTATTGTGTGCATATAACACCATGTTCTATCTCCCTTTTCAACAAAGACTGATAATCTTGCTCTGCCTTATGTCGATTACTTGCCTATTGAGCATCTTTTGGGCAAACTGTTATTATGACAAGAATTTAGAATTTATATCAATACTCCTTTAAA
CDS Sequence
- >LOC_Os01g45760.2
ATGGACAACAAGCCGGCGCAAGAACGCCGCGAGACGTGGGTCCCGGGCGCCGTCATCGTCGGCGCGGGCCCGTCGGGGCTCGCCGCGGCGGCGTGCCTCGCGGCGCGGGGCGTTCCGGCGACGGTGCTTGAGCGGTCCGACTCGCTGGCGTCCACGTGGCGCCACCGCATGTACGACCGCCTCGCTCTCCACCTGCCCAAGCGCTTCTGCGAGCTGCCGCTCCTCCCGTTCCCGGAGGAGTACCCGACGTACCCGTCCAAGGACCAGTTCGTGGCGTACATGGAGGCGTACGCCGCGGCGGCGGGCGTCGCGCCGCGCTTCGGCGCCACCGTCGAGGAGGCCGCGTTCGACGCGGCCGTCGGCGCATGGAGGGTGCGTCTCGACGGCGGGGAGGTGCTCATGGCGCGGTGGCTCGTCGTCGCGACGGGGGAGAACGCCGAGCCGCGGGTGCCGGACTTTCCCGGCATGCAGAAGTTCGCCGGGTGTGCCATGCATACTTCCGAGTACAAGTCCGGCGAGCAGTTTGCCGGTAAGAAGGTGTTGGTCGTGGGATGCGGGAATTCTGGCATGGAAGTGAGCTTGGATTTGTGCCGGCATGGCGCGAAGCCCTCCATGGTGGTGCGCAACACGGTGCATGTCCTACCAAGGGAAATGTTCGGTCTATCCACGTTCGGCATCGCCATGGCGCTGCTGCGATGGCTACCGGTTCAGCTCGTCGACCGGTTCCTTCTCACGGCGGCTCACCTCATCCTCGGTAACACGGGGCAGTTCGGCCTAAGGCGGCCCAAAACAGGGCCCATCGAGCTCAAGAACCTCACCGGCAGGACACCCGTACTGGACGTTGGGACGCTAGACCACATCAAATCCGGCAAAATTAAGGTAGTGGGAGCAGTGAAGGAGATGACGAGGCAAGGGGTCAGGTTCACGGATGGCAAGGAGGAGCAATTCGACACAATAATCCTCGCCACAGGCTACAGGAGCAACGTGCCATCTTGGCTCAAGGTAAAGCAGAGTGTGACTCACTCTTGTTCGTTTTATTTTTCTTTCTGTCCGCACTGCACTATACTACTACTAGTGATATATATCTCGAGATGA - >LOC_Os01g45760.1
ATGGACAACAAGCCGGCGCAAGAACGCCGCGAGACGTGGGTCCCGGGCGCCGTCATCGTCGGCGCGGGCCCGTCGGGGCTCGCCGCGGCGGCGTGCCTCGCGGCGCGGGGCGTTCCGGCGACGGTGCTTGAGCGGTCCGACTCGCTGGCGTCCACGTGGCGCCACCGCATGTACGACCGCCTCGCTCTCCACCTGCCCAAGCGCTTCTGCGAGCTGCCGCTCCTCCCGTTCCCGGAGGAGTACCCGACGTACCCGTCCAAGGACCAGTTCGTGGCGTACATGGAGGCGTACGCCGCGGCGGCGGGCGTCGCGCCGCGCTTCGGCGCCACCGTCGAGGAGGCCGCGTTCGACGCGGCCGTCGGCGCATGGAGGGTGCGTCTCGACGGCGGGGAGGTGCTCATGGCGCGGTGGCTCGTCGTCGCGACGGGGGAGAACGCCGAGCCGCGGGTGCCGGACTTTCCCGGCATGCAGAAGTTCGCCGGGTGTGCCATGCATACTTCCGAGTACAAGTCCGGCGAGCAGTTTGCCGGTAAGAAGGTGTTGGTCGTGGGATGCGGGAATTCTGGCATGGAAGTGAGCTTGGATTTGTGCCGGCATGGCGCGAAGCCCTCCATGGTGGTGCGCAACACGGTGCATGTCCTACCAAGGGAAATGTTCGGTCTATCCACGTTCGGCATCGCCATGGCGCTGCTGCGATGGCTACCGGTTCAGCTCGTCGACCGGTTCCTTCTCACGGCGGCTCACCTCATCCTCGGTAACACGGGGCAGTTCGGCCTAAGGCGGCCCAAAACAGGGCCCATCGAGCTCAAGAACCTCACCGGCAGGACACCCGTACTGGACGTTGGGACGCTAGACCACATCAAATCCGGCAAAATTAAGGTAGTGGGAGCAGTGAAGGAGATGACGAGGCAAGGGGTCAGGTTCACGGATGGCAAGGAGGAGCAATTCGACACAATAATCCTCGCCACAGGCTACAGGAGCAACGTGCCATCTTGGCTCAAGGTAAAGCAGAGTGTGACTCACTCTTGTTCGTTTTATTTTTCTTTCTGTCCGCACTGCACTATACTACTACTAGTGATATATATCTCGAGATGA
Protein Sequence
- >LOC_Os01g45760.2
MDNKPAQERRETWVPGAVIVGAGPSGLAAAACLAARGVPATVLERSDSLASTWRHRMYDRLALHLPKRFCELPLLPFPEEYPTYPSKDQFVAYMEAYAAAAGVAPRFGATVEEAAFDAAVGAWRVRLDGGEVLMARWLVVATGENAEPRVPDFPGMQKFAGCAMHTSEYKSGEQFAGKKVLVVGCGNSGMEVSLDLCRHGAKPSMVVRNTVHVLPREMFGLSTFGIAMALLRWLPVQLVDRFLLTAAHLILGNTGQFGLRRPKTGPIELKNLTGRTPVLDVGTLDHIKSGKIKVVGAVKEMTRQGVRFTDGKEEQFDTIILATGYRSNVPSWLKVKQSVTHSCSFYFSFCPHCTILLLVIYISR* - >LOC_Os01g45760.1
MDNKPAQERRETWVPGAVIVGAGPSGLAAAACLAARGVPATVLERSDSLASTWRHRMYDRLALHLPKRFCELPLLPFPEEYPTYPSKDQFVAYMEAYAAAAGVAPRFGATVEEAAFDAAVGAWRVRLDGGEVLMARWLVVATGENAEPRVPDFPGMQKFAGCAMHTSEYKSGEQFAGKKVLVVGCGNSGMEVSLDLCRHGAKPSMVVRNTVHVLPREMFGLSTFGIAMALLRWLPVQLVDRFLLTAAHLILGNTGQFGLRRPKTGPIELKNLTGRTPVLDVGTLDHIKSGKIKVVGAVKEMTRQGVRFTDGKEEQFDTIILATGYRSNVPSWLKVKQSVTHSCSFYFSFCPHCTILLLVIYISR*