Gene Details:

Functional Descriptions:

  • Here we show that a locus for indica-japonica hybrid male sterility, Sa, comprises two adjacent genes, SaM and SaF, encoding a small ubiquitin-like modifier E3 ligase-like protein and an F-box protein, respectively.
  • This allele-specific gamete elimination results from a selective interaction of SaF(+) with SaM(-), a truncated protein, but not with SaM(+) because of the presence of an inhibitory domain, although SaM(+) is required for this male sterility.
  • The complex locus Sa comprises two adjacently located genes, SaF and SaM, which interact to cause abortion of pollen grains carrying the japonica allele in japonica-indica hybrids.
  • Our results firmly proved that SaF and SaM are required for the hybrid male sterility, but are not essential for pollen development.
  • Here we showed that silencing of SaF or SaM by RNA interference restored male fertility in indica-japonica hybrids with heterozygous Sa.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os01g39670.1
    ATGGAGGCGGTCATCGCGGAGTGCCGCCCCAAGCCTCTCTTCACCACCGGGCCCTTCCTCTCCGCCGTCGGCGGCGGCGGCGGCGGCGTCGACCGCATCTCCGGGCTCCCCGACGACCTCCTCTTTGTCATCCTCTCCAAGCTCCCCGTCAGGGATGCCGTGGCGACGTCCGCCCTCTCCCCCCGCTGGAAGAGCCTCTGGTCCAGCGTCCCGCTCCGCCTCGACGACGCCGGCCTCCTCCACCGCCGCGACGGCACGCGCCTCGGCCGGGAAGGCGTCGCCGCCACCGTCTCCGCCGTCCTCGCCGCCCACCCAGGCCCCGTGCCCGCGGCCTCCGTTGGGTGCTGCCTCTCCTCCGACGACCAGGGCTACCAGCTGGGAGGCTGGCTCCGAGCCCTCGCCGCCAAGGGCGTTCGGCAACTGTGCCTGATGGGCGCGCCATGGTCGCCGCGCGCTGCTCTCCCCTCCGCTGTGTTCTCCTGCTCATCCCTGCGGCGCCTCTTCCTTGGTTCCGTGCAATGCAACTGGGACCTCATACCAGACCATGCCTGCTTCCCTGAGCTACGAGAGATCCAGATATGCAACGCCCTAATGAAGAGCCAGGATCTGTCCCTTGTTTTGGCTGTCTGCCCAGCGCTTGAGACAGTGGAGATTCTTGCGAGCCGTAACAAAATCCCCACCGTGCGAATGAGTAGCCATACTATCCGCAATACTTTGCTATGGAAGTCGGTGGCCAAGGAAGTGAACGTTTTGGACACCCCTTGCCTCAGCAGGGTTGTTTTGTGGCAGGATCTTCTGCTGCCCCACTCAAGATATAATTCTAAGGTCACAATCAGCCGTGCCACCAAAATGCGCATTTCCGGTTACCTGGACACCGGCATCAACACTTTGGTGATCAACGAAACCACAGTTAAGGTAAACACAAATATAAGCTTCAAGACACTTATCCCCAGCGTGAAGGTTTTGGGTCTCTCTGTTCATTTCGGAGTACGAAAGGAGGCTTTGATGTCCATCAGCTTCCTTAGGTGTTTTCCAGAGGTTGAGACACTACATATAACTAGCAAAACGGACAAGGCTTCAGAAGCCGAACAATTCAGTTTCTGGGGAAAGGTTGATCCTGTTGAGTGTGTGACCTCTCATCTCAAGAAGTTAGTGTTCCATGGGATGCCATGGTGTCCTGGAAACCTTGAGTTTCTCAAATTCATCGTGGAAGGGGCATACTTGCTAGAGAAGGTGTTGATTGTTCTTCCTAAAGGAACTTACACTAGCATGCATAGCGTTATCACCAAACTCAAGTTAGCACCTTTGACCTCTGCAAGTTGGGCTAGTCACATCTGTAAAATGGAGGTTGTCCAATCCAGTCAAGGAACTTTGAGCTACCAAAGGGCATCTGATCATTCCGTCGATGATCCTTTAGATTATTCCCTGTAGGCTGTTCTATCTGTTTGTTGTGATTATAACATATTGTCGATATGGTTATCCTTTAGGTCCTTCATGTCTGTATGCCATTTTCATGTGTTTTGGCTTGTTATCTAGAGAAAAATGTATGGTTTGTTGGATATGCTTAGTACTATACTTGATGGGGTGCAAGTAATAGAATGCAGAGCATCCGATGTGTCAGTAGTTCCTCTCCATTACTTCTTGTGGCAAGACAGCAAGATACTCTACTCTCGATTTTGTTGCC
CDS Sequence
  • >LOC_Os01g39670.1
    ATGGAGGCGGTCATCGCGGAGTGCCGCCCCAAGCCTCTCTTCACCACCGGGCCCTTCCTCTCCGCCGTCGGCGGCGGCGGCGGCGGCGTCGACCGCATCTCCGGGCTCCCCGACGACCTCCTCTTTGTCATCCTCTCCAAGCTCCCCGTCAGGGATGCCGTGGCGACGTCCGCCCTCTCCCCCCGCTGGAAGAGCCTCTGGTCCAGCGTCCCGCTCCGCCTCGACGACGCCGGCCTCCTCCACCGCCGCGACGGCACGCGCCTCGGCCGGGAAGGCGTCGCCGCCACCGTCTCCGCCGTCCTCGCCGCCCACCCAGGCCCCGTGCCCGCGGCCTCCGTTGGGTGCTGCCTCTCCTCCGACGACCAGGGCTACCAGCTGGGAGGCTGGCTCCGAGCCCTCGCCGCCAAGGGCGTTCGGCAACTGTGCCTGATGGGCGCGCCATGGTCGCCGCGCGCTGCTCTCCCCTCCGCTGTGTTCTCCTGCTCATCCCTGCGGCGCCTCTTCCTTGGTTCCGTGCAATGCAACTGGGACCTCATACCAGACCATGCCTGCTTCCCTGAGCTACGAGAGATCCAGATATGCAACGCCCTAATGAAGAGCCAGGATCTGTCCCTTGTTTTGGCTGTCTGCCCAGCGCTTGAGACAGTGGAGATTCTTGCGAGCCGTAACAAAATCCCCACCGTGCGAATGAGTAGCCATACTATCCGCAATACTTTGCTATGGAAGTCGGTGGCCAAGGAAGTGAACGTTTTGGACACCCCTTGCCTCAGCAGGGTTGTTTTGTGGCAGGATCTTCTGCTGCCCCACTCAAGATATAATTCTAAGGTCACAATCAGCCGTGCCACCAAAATGCGCATTTCCGGTTACCTGGACACCGGCATCAACACTTTGGTGATCAACGAAACCACAGTTAAGGTAAACACAAATATAAGCTTCAAGACACTTATCCCCAGCGTGAAGGTTTTGGGTCTCTCTGTTCATTTCGGAGTACGAAAGGAGGCTTTGATGTCCATCAGCTTCCTTAGGTGTTTTCCAGAGGTTGAGACACTACATATAACTAGCAAAACGGACAAGGCTTCAGAAGCCGAACAATTCAGTTTCTGGGGAAAGGTTGATCCTGTTGAGTGTGTGACCTCTCATCTCAAGAAGTTAGTGTTCCATGGGATGCCATGGTGTCCTGGAAACCTTGAGTTTCTCAAATTCATCGTGGAAGGGGCATACTTGCTAGAGAAGGTGTTGATTGTTCTTCCTAAAGGAACTTACACTAGCATGCATAGCGTTATCACCAAACTCAAGTTAGCACCTTTGACCTCTGCAAGTTGGGCTAGTCACATCTGTAAAATGGAGGTTGTCCAATCCAGTCAAGGAACTTTGAGCTACCAAAGGGCATCTGATCATTCCGTCGATGATCCTTTAGATTATTCCCTGTAG
Protein Sequence
  • >LOC_Os01g39670.1
    MEAVIAECRPKPLFTTGPFLSAVGGGGGGVDRISGLPDDLLFVILSKLPVRDAVATSALSPRWKSLWSSVPLRLDDAGLLHRRDGTRLGREGVAATVSAVLAAHPGPVPAASVGCCLSSDDQGYQLGGWLRALAAKGVRQLCLMGAPWSPRAALPSAVFSCSSLRRLFLGSVQCNWDLIPDHACFPELREIQICNALMKSQDLSLVLAVCPALETVEILASRNKIPTVRMSSHTIRNTLLWKSVAKEVNVLDTPCLSRVVLWQDLLLPHSRYNSKVTISRATKMRISGYLDTGINTLVINETTVKVNTNISFKTLIPSVKVLGLSVHFGVRKEALMSISFLRCFPEVETLHITSKTDKASEAEQFSFWGKVDPVECVTSHLKKLVFHGMPWCPGNLEFLKFIVEGAYLLEKVLIVLPKGTYTSMHSVITKLKLAPLTSASWASHICKMEVVQSSQGTLSYQRASDHSVDDPLDYSL*