Gene Details:
- MSU gene ID: LOC_Os01g34880 ,LOC_Os01g34890
- RAPdb gene ID: Os01g0533000
- Gene Symbol: SEM1
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- Map-based cloning identified two allelic mutations in a gene encoding a GLUCAN SYNTHASE-LIKE (GSL) protein, thus indicating a role for SEM1 in callose biosynthesis.
- In situ expression analysis and GUS staining showed that SEM1 was mainly expressed in vascular phloem cells.
- Plasma membrane-localized SEM1 protein mediates sugar movement to sink rice tissues.
- Subcellular localization in rice showed that SEM1 localized to the plasma membrane.
- Map-based cloning identified two allelic mutations in a gene encoding a GLUCAN SYNTHASE-LIKE (GSL) protein, thus indicating a role for SEM1 in callose biosynthesis.
- Reduced sucrose transport was found in the SEM1-1/1-2 mutant, which led to excessive starch accumulation in source leaves and inhibited photosynthesis.
Function-related keywords:
- map-based-cloning , phloem , sugar , plasma-membrane , callose-biosynthesis , starch-accumulation , sucrose-transport , photosynthesis
Literature:
- Plasma membrane-localized SEM1 protein mediates sugar movement to sink rice tissues . DOI: 10.1111/tpj.15573 ; PMID: 34750914
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os01g34880.1
ATGAGAGAAGGGAATGTCACACATCATGAGTATATGCAAGTTGGTAAGGGGCGTGATGTGGGAATGAATCAAATTTCAAGCTTTGAAGCTAAGGTGGCAAATGGCAATGGTGAACAAACATTGAGTCGTGATATCTATCGGCTGGGGCGCAGATTCGATTTTTATAGAATGCTATCCTTCTACTTTACTACAGTTGGGTTTTACTTTAGTAGCATGGTTACAGTACTTACAGTTTATGTGTTTCTATATGGGAGGTTATATCTAGTTATGAGTGGTCTGGAAAGGTCTATTTTGCTGGATCCACGTATCGAGCAGAACATTAAGCCTCTTGAAAACGCACTGGCCTCGCAGTCTTTTTTCCAGCTAGGTTTGCTGCTTGTTCTCCCTATGGTAATGGAAGTTGGCTTAGAAAAGGGATTCCGCACAGCATTAGGAGAGTTTGTTATCATGCAGCTTCAGCTGGCATCTGTGTTTTTCACATTCCAGCTTGGCACCAAAACGCACTACTACGGAAGAACAATACTGCATGGCGGGGCTAAATACAGACCTACAGGTCGTGGATTTGTTGTCTACCATGCAAAGTTTGCTGACAATTACCGCATGTACTCCCGCAGCCACTTTGTTAAAGGACTTGAACTGTTGATACTTCTGGTGGTTTATTTAGTGTATGGAAGTTCCTACCGCAGCTCAAGCATGTACCTATTTGTCACCTTCTCCATATGGTTCCTGGTTGCATCTTGGCTCTTCGCACCCTTTATCTTCAATCCGTCATGCTTCGAATGGCAGAAGACAGTTGATGACTGGACAGATTGGAGGAAGTGGATGGGGAACCGTGGGGGTATCGGCATGTCTGTGGATCAAAGCTGGGAGGCTTGGTGGATAAGTGAGCAGGAGCACCTTAGGAAGACTAGCATTCGCTCTCTCCTCTTGGAAATCATTCTTTCGCTCCGCTTCTTGATCTATCAGTACGGTATTGTGTACCATCTGAACATAGCACGCCGCAGCAAAAGCATTCTGGTATATGGATTGTCATGGCTGGTTATGCTCTCAGTCCTAGTAGTTCTAAAGATGGTTTCGATTGGGCGACAGAAGTTTGGAACAGATCTGCAGCTTATGTTCCGCATTCTCAAGGGCCTCCTGTTCCTTGGTTTTGTCTCTGTGATGGCTGTATTATTCGTCGTTTGTAACCTCACAATTTCGGATGTTTTTGCTAGTATTCTTGGGTTCATGCCTACTGGTTGGTGCATTCTTCTGATTGGGCAGGCATGTTCGCCGTTGGTGAAGAAGGCAATGCTGTGGGATTCCATCATGGAGCTCGGGAGGTCGTACGAGAACCTGATGGGACTCGTCCTCTTCCTCCCCATCGGCCTCTTGTCATGGTTCCCCTTCGTGTCCGAGTTCCAGACACGACTGCTCTTCAACCAGGCCTTCAGCCGTGGCCTCCAGATCTCAAGGATCCTCGCCGGGCAAAAGGACATTGGAGAGGAATGAATGAATGGATGAATGGCCCCTCCTGGCCTCGTCTCCTCCGGTGAACTCGCTTGGACGAAAAAACCATCGATTTTGGGCGGCTAGTTCGTTGATAGGGCCTTTTTTAAATAGTTCTGAACAAACAAATAGTAGCCGGTACATCTGAACTGTAGTATAAATGTTTGTTCCTCTTTAGAACAAGGCATATATATGTAGTAAGCAATTAACATAAATGCAGTACGTTGGTATATTTCC
CDS Sequence
- >LOC_Os01g34880.1
ATGAGAGAAGGGAATGTCACACATCATGAGTATATGCAAGTTGGTAAGGGGCGTGATGTGGGAATGAATCAAATTTCAAGCTTTGAAGCTAAGGTGGCAAATGGCAATGGTGAACAAACATTGAGTCGTGATATCTATCGGCTGGGGCGCAGATTCGATTTTTATAGAATGCTATCCTTCTACTTTACTACAGTTGGGTTTTACTTTAGTAGCATGGTTACAGTACTTACAGTTTATGTGTTTCTATATGGGAGGTTATATCTAGTTATGAGTGGTCTGGAAAGGTCTATTTTGCTGGATCCACGTATCGAGCAGAACATTAAGCCTCTTGAAAACGCACTGGCCTCGCAGTCTTTTTTCCAGCTAGGTTTGCTGCTTGTTCTCCCTATGGTAATGGAAGTTGGCTTAGAAAAGGGATTCCGCACAGCATTAGGAGAGTTTGTTATCATGCAGCTTCAGCTGGCATCTGTGTTTTTCACATTCCAGCTTGGCACCAAAACGCACTACTACGGAAGAACAATACTGCATGGCGGGGCTAAATACAGACCTACAGGTCGTGGATTTGTTGTCTACCATGCAAAGTTTGCTGACAATTACCGCATGTACTCCCGCAGCCACTTTGTTAAAGGACTTGAACTGTTGATACTTCTGGTGGTTTATTTAGTGTATGGAAGTTCCTACCGCAGCTCAAGCATGTACCTATTTGTCACCTTCTCCATATGGTTCCTGGTTGCATCTTGGCTCTTCGCACCCTTTATCTTCAATCCGTCATGCTTCGAATGGCAGAAGACAGTTGATGACTGGACAGATTGGAGGAAGTGGATGGGGAACCGTGGGGGTATCGGCATGTCTGTGGATCAAAGCTGGGAGGCTTGGTGGATAAGTGAGCAGGAGCACCTTAGGAAGACTAGCATTCGCTCTCTCCTCTTGGAAATCATTCTTTCGCTCCGCTTCTTGATCTATCAGTACGGTATTGTGTACCATCTGAACATAGCACGCCGCAGCAAAAGCATTCTGGTATATGGATTGTCATGGCTGGTTATGCTCTCAGTCCTAGTAGTTCTAAAGATGGTTTCGATTGGGCGACAGAAGTTTGGAACAGATCTGCAGCTTATGTTCCGCATTCTCAAGGGCCTCCTGTTCCTTGGTTTTGTCTCTGTGATGGCTGTATTATTCGTCGTTTGTAACCTCACAATTTCGGATGTTTTTGCTAGTATTCTTGGGTTCATGCCTACTGGTTGGTGCATTCTTCTGATTGGGCAGGCATGTTCGCCGTTGGTGAAGAAGGCAATGCTGTGGGATTCCATCATGGAGCTCGGGAGGTCGTACGAGAACCTGATGGGACTCGTCCTCTTCCTCCCCATCGGCCTCTTGTCATGGTTCCCCTTCGTGTCCGAGTTCCAGACACGACTGCTCTTCAACCAGGCCTTCAGCCGTGGCCTCCAGATCTCAAGGATCCTCGCCGGGCAAAAGGACATTGGAGAGGAATGA
Protein Sequence
- >LOC_Os01g34880.1
MREGNVTHHEYMQVGKGRDVGMNQISSFEAKVANGNGEQTLSRDIYRLGRRFDFYRMLSFYFTTVGFYFSSMVTVLTVYVFLYGRLYLVMSGLERSILLDPRIEQNIKPLENALASQSFFQLGLLLVLPMVMEVGLEKGFRTALGEFVIMQLQLASVFFTFQLGTKTHYYGRTILHGGAKYRPTGRGFVVYHAKFADNYRMYSRSHFVKGLELLILLVVYLVYGSSYRSSSMYLFVTFSIWFLVASWLFAPFIFNPSCFEWQKTVDDWTDWRKWMGNRGGIGMSVDQSWEAWWISEQEHLRKTSIRSLLLEIILSLRFLIYQYGIVYHLNIARRSKSILVYGLSWLVMLSVLVVLKMVSIGRQKFGTDLQLMFRILKGLLFLGFVSVMAVLFVVCNLTISDVFASILGFMPTGWCILLIGQACSPLVKKAMLWDSIMELGRSYENLMGLVLFLPIGLLSWFPFVSEFQTRLLFNQAFSRGLQISRILAGQKDIGEE*