Information report for OsSar1a
Gene Details
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Functional Descriptions
- However, simultaneous knockdown of OsSar1a/b/c resulted in floury and shrunken seeds, with an increased level of glutelin precursor and decreased level of the mature alpha- and beta-subunit.
- To reveal the route by which glutelin and alpha-globulin exit the ER, four putative Sar1 genes (OsSar1a/b/c/d) were cloned from rice, and transgenic rice were generated with Sar1 overexpressed or suppressed by RNA interference (RNAi) specifically in the endosperm under the control of the rice glutelin promoter.
- OsSar1abc RNAi endosperm generated numerous, spherical, novel protein bodies with highly electron-dense matrixes containing both glutelin and alpha-globulin.
- These results indicated that OsSar1a/b/c play a crucial role in storage proteins exiting from the ER, with functional redundancy in rice endosperm, and glutelin and alpha-globulin transported together from the ER to the Golgi apparatus by a pathway mediated by coat protein complex II.
Literature and News
- Small GTPase Sar1 is crucial for proglutelin and α-globulin export from the endoplasmic reticulum in rice endosperm . DOI: 10.1093/jxb/ert128 ; PMID: 23682119
- A deleterious Sar1c variant in rice inhibits export of seed storage proteins from the endoplasmic reticulum . DOI: 10.1007/s11103-022-01327-z ; PMID: 36469200
Gene Resources
- UniProt: A0A8J8Y1A8
- EMBL: AP003331, AP003793, AP008207
- EnsemblPlants: Os01t0338000-01, Os01t0338000-02
- Gramene: Os01t0338000-01, Os01t0338000-02
- KEGG: dosa:Os01g0338000, osa:4326797
- Orthologous matrix: DCADYER
- InterPro: IPR005225, IPR006687, IPR006689
- PANTHER: PTHR45684
- SUPFAM: SSF52540
- PROSITE: PS51417, PS51422
- Gene3D: 3.40.50.300
- OrthoDB: A0A8J8Y1A8
- SWISS-MODEL: A0A8J8Y1A8
- Conserved Domain Database: cd00879
Sequences
cDNA Sequence
- >LOC_Os01g23620.1
CTGTTTCTAGTTGAGGGGAAGTCGATCGGGATTAGGAGGAAGCCGGGCCCACATGCAGGCGCCCACACCCACACTTCAATCCTCACGCCCCAGCAGTTAATGCCTTGACGACCCTCCTCCCCTCTCCCCAACCAAGCATAGAATAGGCGGACACACAGGGTGATAGAGAGAGAGAGATTCAGATCCACCCGCGTCTTCTCTTCTCCCCTCTCGTCCACCCATCCCTCGATCGATCCGGCGATCTCCGCCGCCGCCGCCGCCGCCGCTAGCAGCAGCAACTGCACTAGGTAAAGAGGAGGAGAGGAGAGGAGAGGGCGCGCGGTACAGAGGAGGAAGAGGCGTGGGGAGAGAAGATGTTCCTGTGGGACTGGTTCTACGGCGTGCTCGCGTCGCTGGGGCTGTGGCAGAAGGAGGCCAAGATCCTCTTCCTCGGCCTCGACAACGCCGGCAAGACCACCCTCCTCCACATGCTCAAGGACGAGAGGTTGGTGCAGCACCAGCCGACGCAGCACCCGACGTCGGAGGAGCTCAGCATCGGGAAGATCAAGTTCAAGGCCTTCGATCTCGGCGGCCACCAGATCGCCCGCCGCGTCTGGAAGGACTACTACGCCAAGGTTGATGCTGTGGTATATTTGGTAGATGCTTATGATAAGGAACGTTTTGCTGAGTCAAAAAAGGAGCTTGATGCACTCCTATCTGATGATTCATTGGCAGCTGTCCCGTTTCTTATCCTTGGCAACAAGATTGACATTCCATATGCTGCTTCTGAAGAGGAGCTGCGGTATCATCTAGGCCTTAGCAACTTCACAACTGGGAAGGGCAAGGTCAGCCTAGGTGAATCCAATGTCCGGCCCCTCGAAGTGTTCATGTGCAGTGTTGTCCGCAAGATGGGCTATGGTGACGGGTTCAAGTGGGTCTCCCAGTACATCAAGTAGATCCCTTGTCAAGACATACTTGCATACTCTGCCCAGCGATTTTTTTTCCTTCTCGTTTGTTTCAGTTGGTTGTTCTTCACGACAACAATCATCATGACGGTATGGAGGGTATGAAGAGAAAACAGTGCTTTAACAATTGGGGACGATGTTCCATGTTGGATATTTAGCCTCAAACCATTTGTTATGTCGAGCTTCTAAAATTTTTTTGCTTTTCCATAGGGCGTTTACTAAGGACTTGGAAGATGATGAATTATAAGATTATTGGTTTCTTAAATGTTGCTTTGCTTATGGATTGCTGCATGATGTGTTCCCATTGCGATG
CDS Sequence
- >LOC_Os01g23620.1
ATGTTCCTGTGGGACTGGTTCTACGGCGTGCTCGCGTCGCTGGGGCTGTGGCAGAAGGAGGCCAAGATCCTCTTCCTCGGCCTCGACAACGCCGGCAAGACCACCCTCCTCCACATGCTCAAGGACGAGAGGTTGGTGCAGCACCAGCCGACGCAGCACCCGACGTCGGAGGAGCTCAGCATCGGGAAGATCAAGTTCAAGGCCTTCGATCTCGGCGGCCACCAGATCGCCCGCCGCGTCTGGAAGGACTACTACGCCAAGGTTGATGCTGTGGTATATTTGGTAGATGCTTATGATAAGGAACGTTTTGCTGAGTCAAAAAAGGAGCTTGATGCACTCCTATCTGATGATTCATTGGCAGCTGTCCCGTTTCTTATCCTTGGCAACAAGATTGACATTCCATATGCTGCTTCTGAAGAGGAGCTGCGGTATCATCTAGGCCTTAGCAACTTCACAACTGGGAAGGGCAAGGTCAGCCTAGGTGAATCCAATGTCCGGCCCCTCGAAGTGTTCATGTGCAGTGTTGTCCGCAAGATGGGCTATGGTGACGGGTTCAAGTGGGTCTCCCAGTACATCAAGTAG
Protein Sequence
- >LOC_Os01g23620.1
MFLWDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVDAVVYLVDAYDKERFAESKKELDALLSDDSLAAVPFLILGNKIDIPYAASEEELRYHLGLSNFTTGKGKVSLGESNVRPLEVFMCSVVRKMGYGDGFKWVSQYIK*