Gene Details:

Functional Descriptions:

  • Overexpression of OsIFL in transgenic tobacco plants conferred salinity stress tolerance by maintaining favourable K(+)/Na(+) ratio and thus showed protection from salinity stress induced ion toxicity.
  • Our investigations clearly establish that the heterologous expression of OsIFL in three diverse organisms (bacteria, yeast and tobacco) provides survival advantage towards diverse abiotic stresses.
  • Seeds of OsIF overexpression rice germinated normally in the presence of high salt, showed better growth, maintained chloroplast ultrastructure and favourable K+/Na+ ratio than the wild type (WT) and KD plants.
  • Under salinity and high temperature stress, OsIF overexpressing plants could maintain significantly high yield, while the WT and KD plants could not.
  • Overexpression of OsIF in transgenic rice enhanced tolerance to salinity and heat stress, while its knock-down (KD) rendered plants more sensitive thereby indicating the role of IFs in promoting survival under stress.
  • Analysis of photosynthesis and chlorophyll a fluorescence data suggested better performance of both photosystem I and II in the OsIF overexpression rice under salinity stress as compared to the WT and KD.
  • Further, metabolite profiling revealed a 2-4 fold higher accumulation of proline and trehalose in OsIF overexpressing rice than WT, under salinity stress.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os01g18840.1
    ATGCCGATTTCAGAGAGGGCAACAACAACGCCATCATCGTCATATTACTCGTTGGAGTTGGATCCACTGTTGAGTGATCTCGCAGAGAAGAAGCTTAGCCTGAGGAGAAGCTTGGCTTGGCTGGATGCAGAGCTCAAGGATGCCAAGATCAAGCTTGCTTCCAAGGAACAGTTGCTTGCTCAGGAATCAGAAAACAGGAAGAAGTTTGCTGAGTCGAGGGCGAGAAGCATGGAGGAGGAGGTGAAGAAGCTGCATAAATGTTTACAGGACAAGGATGAGCAGCTGCGAACATCAATATGTAGCACTGAACAGCAGTATTTGTCTTCCTACAAATTGGATATTCTTAGATCACAGATTTCAGTGGCACAAGCCACTGCAGAAGCCAGTGCAGAATCAGCCATGTTGGCACGGTTGCAGTGCTTGTCCCTGTCAGGAGGGCATGAGAAGATCAATTCTTTGGGTGAATGCGAGCTTCGAGTGAAGAAGGTAGAGGAGCAGCTAGATCTTGTTCAGAAGTTCCTTGAAGCGAAAGAGCTCTCGCAGTTGGAGAAGAATCAAATGACAACGGTTCATGAGCTGAAGAAGAAGGTACTGAAACTTGAATGCACCCTCAAAGTATCAAGAGCTCAGCTGCGAAAACTACATAAGATGGTAGAGCGAAGAGATAAACCTCTCAAAAAACTACAAAGCCGATTGCCGCTGAAGCAGCAGACCGCCTGCGACAAGCAGAAGTTGTGGGAGAGCTCAGGCTTCAGGATCATAGCCTCCATGTCAATATTGGCCCTGGCTATGCTGTCGAAGCGATGAACTGAACAAGTGATGATTGACGTCGTCGTGTATCAGTTGTCTATGGCAGACGCAAATGTTCCTGTATTGGTGTCAGGGTTATGAGATAGTTGATGGTATGAATGATAAATCTGTGTAGCATCATTACCAGTTCTCCGTACCAAATTTGACGAAATTCAGTGTACTGTCGTAGACTTCAGCGTGGTGGAAACTTAAACCAATGCACTAAATGAACTTGGGG
CDS Sequence
  • >LOC_Os01g18840.1
    ATGCCGATTTCAGAGAGGGCAACAACAACGCCATCATCGTCATATTACTCGTTGGAGTTGGATCCACTGTTGAGTGATCTCGCAGAGAAGAAGCTTAGCCTGAGGAGAAGCTTGGCTTGGCTGGATGCAGAGCTCAAGGATGCCAAGATCAAGCTTGCTTCCAAGGAACAGTTGCTTGCTCAGGAATCAGAAAACAGGAAGAAGTTTGCTGAGTCGAGGGCGAGAAGCATGGAGGAGGAGGTGAAGAAGCTGCATAAATGTTTACAGGACAAGGATGAGCAGCTGCGAACATCAATATGTAGCACTGAACAGCAGTATTTGTCTTCCTACAAATTGGATATTCTTAGATCACAGATTTCAGTGGCACAAGCCACTGCAGAAGCCAGTGCAGAATCAGCCATGTTGGCACGGTTGCAGTGCTTGTCCCTGTCAGGAGGGCATGAGAAGATCAATTCTTTGGGTGAATGCGAGCTTCGAGTGAAGAAGGTAGAGGAGCAGCTAGATCTTGTTCAGAAGTTCCTTGAAGCGAAAGAGCTCTCGCAGTTGGAGAAGAATCAAATGACAACGGTTCATGAGCTGAAGAAGAAGGTACTGAAACTTGAATGCACCCTCAAAGTATCAAGAGCTCAGCTGCGAAAACTACATAAGATGGTAGAGCGAAGAGATAAACCTCTCAAAAAACTACAAAGCCGATTGCCGCTGAAGCAGCAGACCGCCTGCGACAAGCAGAAGTTGTGGGAGAGCTCAGGCTTCAGGATCATAGCCTCCATGTCAATATTGGCCCTGGCTATGCTGTCGAAGCGATGA
Protein Sequence
  • >LOC_Os01g18840.1
    MPISERATTTPSSSYYSLELDPLLSDLAEKKLSLRRSLAWLDAELKDAKIKLASKEQLLAQESENRKKFAESRARSMEEEVKKLHKCLQDKDEQLRTSICSTEQQYLSSYKLDILRSQISVAQATAEASAESAMLARLQCLSLSGGHEKINSLGECELRVKKVEEQLDLVQKFLEAKELSQLEKNQMTTVHELKKKVLKLECTLKVSRAQLRKLHKMVERRDKPLKKLQSRLPLKQQTACDKQKLWESSGFRIIASMSILALAMLSKR*