Information report for OsIFL;OsIF
Gene Details
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Functional Descriptions
- Overexpression of OsIFL in transgenic tobacco plants conferred salinity stress tolerance by maintaining favourable K(+)/Na(+) ratio and thus showed protection from salinity stress induced ion toxicity.
- Our investigations clearly establish that the heterologous expression of OsIFL in three diverse organisms (bacteria, yeast and tobacco) provides survival advantage towards diverse abiotic stresses.
- Seeds of OsIF overexpression rice germinated normally in the presence of high salt, showed better growth, maintained chloroplast ultrastructure and favourable K+/Na+ ratio than the wild type (WT) and KD plants.
- Under salinity and high temperature stress, OsIF overexpressing plants could maintain significantly high yield, while the WT and KD plants could not.
- Overexpression of OsIF in transgenic rice enhanced tolerance to salinity and heat stress, while its knock-down (KD) rendered plants more sensitive thereby indicating the role of IFs in promoting survival under stress.
- Analysis of photosynthesis and chlorophyll a fluorescence data suggested better performance of both photosystem I and II in the OsIF overexpression rice under salinity stress as compared to the WT and KD.
- Further, metabolite profiling revealed a 2-4 fold higher accumulation of proline and trehalose in OsIF overexpressing rice than WT, under salinity stress.
Functional Keywords
Literature and News
- Evidence for nuclear interaction of a cytoskeleton protein (OsIFL) with metallothionein and its role in salinity stress tolerance . DOI: 10.1038/srep34762 ; PMID: 27708383
- Rice intermediate filament, OsIF, stabilizes photosynthetic machinery and yield under salinity and heat stress . DOI: 10.1038/s41598-018-22131-0 ; PMID: 29511223
Gene Resources
- UniProt: Q5JNS1
- EMBL: AP002482, AP008207, CM000138
- AlphaFoldDB: Q5JNS1
- EnsemblPlants: Os01t0292700-01
- Gramene: Os01t0292700-01
- KEGG: dosa:Os01g0292700
- InterPro: IPR049932
- PANTHER: PTHR48145, PTHR48145:SF5
- SWISS-MODEL: Q5JNS1
Sequences
cDNA Sequence
- >LOC_Os01g18840.1
ATGCCGATTTCAGAGAGGGCAACAACAACGCCATCATCGTCATATTACTCGTTGGAGTTGGATCCACTGTTGAGTGATCTCGCAGAGAAGAAGCTTAGCCTGAGGAGAAGCTTGGCTTGGCTGGATGCAGAGCTCAAGGATGCCAAGATCAAGCTTGCTTCCAAGGAACAGTTGCTTGCTCAGGAATCAGAAAACAGGAAGAAGTTTGCTGAGTCGAGGGCGAGAAGCATGGAGGAGGAGGTGAAGAAGCTGCATAAATGTTTACAGGACAAGGATGAGCAGCTGCGAACATCAATATGTAGCACTGAACAGCAGTATTTGTCTTCCTACAAATTGGATATTCTTAGATCACAGATTTCAGTGGCACAAGCCACTGCAGAAGCCAGTGCAGAATCAGCCATGTTGGCACGGTTGCAGTGCTTGTCCCTGTCAGGAGGGCATGAGAAGATCAATTCTTTGGGTGAATGCGAGCTTCGAGTGAAGAAGGTAGAGGAGCAGCTAGATCTTGTTCAGAAGTTCCTTGAAGCGAAAGAGCTCTCGCAGTTGGAGAAGAATCAAATGACAACGGTTCATGAGCTGAAGAAGAAGGTACTGAAACTTGAATGCACCCTCAAAGTATCAAGAGCTCAGCTGCGAAAACTACATAAGATGGTAGAGCGAAGAGATAAACCTCTCAAAAAACTACAAAGCCGATTGCCGCTGAAGCAGCAGACCGCCTGCGACAAGCAGAAGTTGTGGGAGAGCTCAGGCTTCAGGATCATAGCCTCCATGTCAATATTGGCCCTGGCTATGCTGTCGAAGCGATGAACTGAACAAGTGATGATTGACGTCGTCGTGTATCAGTTGTCTATGGCAGACGCAAATGTTCCTGTATTGGTGTCAGGGTTATGAGATAGTTGATGGTATGAATGATAAATCTGTGTAGCATCATTACCAGTTCTCCGTACCAAATTTGACGAAATTCAGTGTACTGTCGTAGACTTCAGCGTGGTGGAAACTTAAACCAATGCACTAAATGAACTTGGGG
CDS Sequence
- >LOC_Os01g18840.1
ATGCCGATTTCAGAGAGGGCAACAACAACGCCATCATCGTCATATTACTCGTTGGAGTTGGATCCACTGTTGAGTGATCTCGCAGAGAAGAAGCTTAGCCTGAGGAGAAGCTTGGCTTGGCTGGATGCAGAGCTCAAGGATGCCAAGATCAAGCTTGCTTCCAAGGAACAGTTGCTTGCTCAGGAATCAGAAAACAGGAAGAAGTTTGCTGAGTCGAGGGCGAGAAGCATGGAGGAGGAGGTGAAGAAGCTGCATAAATGTTTACAGGACAAGGATGAGCAGCTGCGAACATCAATATGTAGCACTGAACAGCAGTATTTGTCTTCCTACAAATTGGATATTCTTAGATCACAGATTTCAGTGGCACAAGCCACTGCAGAAGCCAGTGCAGAATCAGCCATGTTGGCACGGTTGCAGTGCTTGTCCCTGTCAGGAGGGCATGAGAAGATCAATTCTTTGGGTGAATGCGAGCTTCGAGTGAAGAAGGTAGAGGAGCAGCTAGATCTTGTTCAGAAGTTCCTTGAAGCGAAAGAGCTCTCGCAGTTGGAGAAGAATCAAATGACAACGGTTCATGAGCTGAAGAAGAAGGTACTGAAACTTGAATGCACCCTCAAAGTATCAAGAGCTCAGCTGCGAAAACTACATAAGATGGTAGAGCGAAGAGATAAACCTCTCAAAAAACTACAAAGCCGATTGCCGCTGAAGCAGCAGACCGCCTGCGACAAGCAGAAGTTGTGGGAGAGCTCAGGCTTCAGGATCATAGCCTCCATGTCAATATTGGCCCTGGCTATGCTGTCGAAGCGATGA
Protein Sequence
- >LOC_Os01g18840.1
MPISERATTTPSSSYYSLELDPLLSDLAEKKLSLRRSLAWLDAELKDAKIKLASKEQLLAQESENRKKFAESRARSMEEEVKKLHKCLQDKDEQLRTSICSTEQQYLSSYKLDILRSQISVAQATAEASAESAMLARLQCLSLSGGHEKINSLGECELRVKKVEEQLDLVQKFLEAKELSQLEKNQMTTVHELKKKVLKLECTLKVSRAQLRKLHKMVERRDKPLKKLQSRLPLKQQTACDKQKLWESSGFRIIASMSILALAMLSKR*