Gene Details:

Functional Descriptions:

  • A membrane-associated NAC transcription factor OsNTL3 is involved in thermotolerance in rice.
  • OsNTL3 encodes a NAC transcription factor with a predicted C-terminal transmembrane domain.
  • Loss-of-function mutation of OsNTL3 confers heat sensitivity while inducible expression of the truncated form of OsNTL3 without the transmembrane domain increases heat tolerance in rice seedlings.
  • In the current study, we discovered that OsNTL3 is required for heat stress tolerance in rice.
  • Interestingly, OsNTL3 directly binds to OsbZIP74 promoter and regulates its expression in response to heat stress.
  • In turn, up-regulation of OsNTL3 by heat stress is dependent on OsbZIP74.
  • Thus, our work reveals the important role of OsNTL3 in thermotolerance, and a regulatory circuit mediated by OsbZIP74 and OsNTL3 in communications among ER, plasma membrane and nucleus under heat stress conditions.
  • RNA-Seq analysis revealed that OsNTL3 regulates the expression of genes involved in ER protein folding and other processes.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os01g15640.1
    CTCCTACCCATCCATCCCTAGCTTCCTCGCTGCTTCGCCCGCCACGTCACCCTCCTCCTCCTCCCCTTTTTTTTCTCTCGCTCGCCTCGCCATCCATGGCGTCGATGGCGAACCAGCAGTAGTAGTAGTCCACCCCAACGACGCGCGCGAGCCAGCTCCGGGGTACCCGCCGCTCGTGCCCGGGGGGGAAGGGGGTGCACCGGGGAGCCGTCGCGCCGCGCGGCTCTGATTCTTCCCGTTCCCGGAGATTTGAACCATGGAGTCTTTACGTGACATGGTCCTCCCACCAGGATTTGGATTCCACCCAAAAGATACTGAGCTCATTTCTCACTATCTGAAAAAGAAAATACATGGCCAAAAGATTGAATATGAGATCATTCCAGAGGTGGATATATACAAGCATGAACCATGGGATTTACCTGCAAAGTGCGATGTTCCAACTCAGGATAATAAGTGGCATTTCTTCGCTGCTCGTGACAGAAAGTATCCTAACGGTTCTCGGTCAAATAGGGCAACAGTTGCTGGGTATTGGAAATCCACTGGAAAGGATCGAGCCATAAAAATGGGCAAGCAAACTATAGGAACGAAGAAAACTTTAGTTTTTCATGAAGGCCGCCCACCCACTGGGAGACGTACTGAGTGGATCATGCATGAGTACTATATAGATGAGCGTGAATGTCAAGCCTGCCCTGATATGAAGGATGCATATGTACTTTGTCGTATTACCAAAAGAAATGACTGGATCCCAGGGAATGGCAATGAGTTGGACAACAGTGATCCTCATCCAGAACCATATGATGCTCCTCCATCAGTTATCAGTACTGAACAGCTAAATCCTGCTGCTGAACCAGTTGTCGGTGTTGAAGCTGCACCAGTTACCGTTGCTGAACCTGACGGTGTTACTACGTCAGCTATCACTGCTAATATACCATCTCCTAGTGATGACATTAATCTGGATGATTGGTTGAATGAACTATTTGATCCTTTCTTTGACCCTGAACAGAGTCTGGCTTCTGCTGATCTGTCTCCAGATGAACAAAATGTTGAATCATCGAACGTAGGTGCTTTGGCTCCAAAGGTGGAACAAGACTATTCTAGTCCTAACGAGAACGTAGTAGATGATACGGAGTACTTGTTGCCTGAAGATGTTTACAACATACTGCACCCTGGTACAGATGACTTCAATATGCTCCAGAATCCTTTGGATCAGTACCCCATTCAATATGCAACCGATGTTTGGAGTGGAATTCAGAAGGAAGAACTGTGGTCGCCCCAGGCCAATGCTGAGCCTAGTCAGTCGAATGAAGCTGCTGATAATGGAATTATAAGACGTTATCGTAGTATGAAAACACCTGAAACTAGTGTGCCGCAGTTCAAGGGTAAAACTCAGGCTAAAATGCGGGTTGGGATCAACAAGATGGCTACGAGTAGTTCTGAATCTATCAACCAGACTATCAAGTTTGAGAACAGTGGTCGTCTTGTTGAGCACCAGAAGAACCAAGCGCATGATGTAGCTTCTACCAAGCGGTCAGACGCAGGGAAGCCAAGTACAGAGCTCAGCAGCAATCGAGGATTTCTCAGGGGTATCCGAAATGCATTTGCAGGCTGTTCGGATGCTCGATGGAACATGATACTTGTTGCGGGTTTCGCTATTGGAGTCGCTGTGGTAGCGCTTCATATAGGCCAACGCCTTGGATTAAGCCAGAGAGATCAGCAGCATACCTAGCCTTTAGGTTTGCAGGTTACTGGGTTTCTATGCTTTTTGCAATCTGGAGAAACGTAAACAAACTCCGATCTTTGGTTGGTGCAGAACATTTGATGTATGCGCCGTTTTTTTTGTGTTGGTTTTGTACATATTATTATCTTCGGTTTTGGTCGACTTTTGTTCTCGCCAGCTAGTAGTACTATGTTGACGAATGTAGAAGAAAACCTCAAAAAAAAAAGAACAAATGAAAAAAAATTGACGATGAAAATTTATATAATTAACCGCTTGCATTGTACTATA
CDS Sequence
  • >LOC_Os01g15640.1
    ATGGAGTCTTTACGTGACATGGTCCTCCCACCAGGATTTGGATTCCACCCAAAAGATACTGAGCTCATTTCTCACTATCTGAAAAAGAAAATACATGGCCAAAAGATTGAATATGAGATCATTCCAGAGGTGGATATATACAAGCATGAACCATGGGATTTACCTGCAAAGTGCGATGTTCCAACTCAGGATAATAAGTGGCATTTCTTCGCTGCTCGTGACAGAAAGTATCCTAACGGTTCTCGGTCAAATAGGGCAACAGTTGCTGGGTATTGGAAATCCACTGGAAAGGATCGAGCCATAAAAATGGGCAAGCAAACTATAGGAACGAAGAAAACTTTAGTTTTTCATGAAGGCCGCCCACCCACTGGGAGACGTACTGAGTGGATCATGCATGAGTACTATATAGATGAGCGTGAATGTCAAGCCTGCCCTGATATGAAGGATGCATATGTACTTTGTCGTATTACCAAAAGAAATGACTGGATCCCAGGGAATGGCAATGAGTTGGACAACAGTGATCCTCATCCAGAACCATATGATGCTCCTCCATCAGTTATCAGTACTGAACAGCTAAATCCTGCTGCTGAACCAGTTGTCGGTGTTGAAGCTGCACCAGTTACCGTTGCTGAACCTGACGGTGTTACTACGTCAGCTATCACTGCTAATATACCATCTCCTAGTGATGACATTAATCTGGATGATTGGTTGAATGAACTATTTGATCCTTTCTTTGACCCTGAACAGAGTCTGGCTTCTGCTGATCTGTCTCCAGATGAACAAAATGTTGAATCATCGAACGTAGGTGCTTTGGCTCCAAAGGTGGAACAAGACTATTCTAGTCCTAACGAGAACGTAGTAGATGATACGGAGTACTTGTTGCCTGAAGATGTTTACAACATACTGCACCCTGGTACAGATGACTTCAATATGCTCCAGAATCCTTTGGATCAGTACCCCATTCAATATGCAACCGATGTTTGGAGTGGAATTCAGAAGGAAGAACTGTGGTCGCCCCAGGCCAATGCTGAGCCTAGTCAGTCGAATGAAGCTGCTGATAATGGAATTATAAGACGTTATCGTAGTATGAAAACACCTGAAACTAGTGTGCCGCAGTTCAAGGGTAAAACTCAGGCTAAAATGCGGGTTGGGATCAACAAGATGGCTACGAGTAGTTCTGAATCTATCAACCAGACTATCAAGTTTGAGAACAGTGGTCGTCTTGTTGAGCACCAGAAGAACCAAGCGCATGATGTAGCTTCTACCAAGCGGTCAGACGCAGGGAAGCCAAGTACAGAGCTCAGCAGCAATCGAGGATTTCTCAGGGGTATCCGAAATGCATTTGCAGGCTGTTCGGATGCTCGATGGAACATGATACTTGTTGCGGGTTTCGCTATTGGAGTCGCTGTGGTAGCGCTTCATATAGGCCAACGCCTTGGATTAAGCCAGAGAGATCAGCAGCATACCTAG
Protein Sequence
  • >LOC_Os01g15640.1
    MESLRDMVLPPGFGFHPKDTELISHYLKKKIHGQKIEYEIIPEVDIYKHEPWDLPAKCDVPTQDNKWHFFAARDRKYPNGSRSNRATVAGYWKSTGKDRAIKMGKQTIGTKKTLVFHEGRPPTGRRTEWIMHEYYIDERECQACPDMKDAYVLCRITKRNDWIPGNGNELDNSDPHPEPYDAPPSVISTEQLNPAAEPVVGVEAAPVTVAEPDGVTTSAITANIPSPSDDINLDDWLNELFDPFFDPEQSLASADLSPDEQNVESSNVGALAPKVEQDYSSPNENVVDDTEYLLPEDVYNILHPGTDDFNMLQNPLDQYPIQYATDVWSGIQKEELWSPQANAEPSQSNEAADNGIIRRYRSMKTPETSVPQFKGKTQAKMRVGINKMATSSSESINQTIKFENSGRLVEHQKNQAHDVASTKRSDAGKPSTELSSNRGFLRGIRNAFAGCSDARWNMILVAGFAIGVAVVALHIGQRLGLSQRDQQHT*