Gene Details:

Functional Descriptions:

  • Transgenic rice lines over-expressing (OX) OsALMT4 released malate from the roots constitutively and had two-fold higher malate concentrations in the xylem sap than nulls indicating greater concentrations of malate in the apoplast.
  • We show that OsALMT4 is expressed in roots and shoots and the OsALMT4 protein localizes to the plasma membrane.
  • Altered Expression of the Malate-Permeable Anion Channel OsALMT4 Reduces the Growth of Rice Under Low Radiance.
  • Growth of transgenic lines with either increased OsALMT4 expression or reduced expression was measured in different environments.
  • Altering OsALMT4 expression compromises growth in low-light environments.
  • Here, we show that OsALMT4 is expressed widely in roots, shoots, flowers, and grain but not guard cells.
  • Malate efflux from the transgenic plants over-expressing OsALMT4 was inhibited by niflumate and salicylic acid.
  • When day-time light was reduced from 700 to 300 mol m-2s-1 independent transgenic lines with either increased or decreased OsALMT4 expression accumulated less biomass compared to their null controls.
  • Previous studies showed that OsALMT4 localizes to the plasma membrane and that expression in transgenic rice lines results in a constitutive release of malate from the roots.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os01g12210.1
    CCAAAGCATACATCAGCTAGACCAGTGTTATTGATCTCTTCTGTGAAACCCTTAGAGCAATTTCTTAAAAGAGACCGCAAGTTAGAGAATGAATGGAAAGAAGGGTAGTATAAGGATTGACATTTGCCTGAAACCAAAAGATGTGGTGCCAGAAGATGCAAAGAAGCCAATCAGTGAAGAAGGGCTTTCCCCAAGGAAATGGCTACATGATGTCTGGGACTTCGCCAGACAGGATACAAACAGGGTCACCTTTGCACTCAAGGTTGGCCTTGCTTGCCTCCTTGTGTCACTGCTCATACTCTTCCGTGCACCCTATGACATCTTTGGGGCTAACATCATCTGGTCCATCCTCACTGTTGCTATAATGTTCGAGTACACTGTTGGTGCAACATTCAATCGCGGATTCAATCGAGCCGTCGGAAGTGTATTTGCTGGAGTATTTGCAGTAGTTGTTATTCAGGTGGCTATGTCTAGTGGCCATATTGCAGAACCATACATCATTGGTTTCAGCATATTTCTCATTGGTGCTGTAACATCATTTATGAAGCTATGGCCATCACTAGTTCCATATGAATATGGTTTTCGAGTTATCCTATTCACATATTGCTTGATCATCGTATCGGGCTACCGCATGGGAAATCCCATCAGGACTGCAATGGACAGACTCTACTCAATTGCGATCGGAGCCCTCATAGCCGTTTTAGTAAATGTCTTTATCTGCCCAATATGGGCAGGGGAGCAACTGCACAGGGAGCTAGTTAACAGCTTCAACTCTCTCGCAGACTCCTTAGAAGAGTGTGTCAAGAAGTATCTAAGTGATGATGGTTCCGAACATCCAGAGTTCTCAAAGACAGTCATGGATAACTTCCCTGATGAACCAGCATTCAGGAAGTGCCGAGCTACGTTAAATTCGTCAGCGAAGTTTGACTCTCTGGCGAACTCTGCTAAATGGGAACCACCACATGGGAGATTCAAGCACTTCTTTTACCCATGGGCAGAGTATGTGAAAGTTGGCAATGTTCTTCGCCACTGTGCCTACGAGGTGATGGCTTTGCATGGTTGCGTGCACTCAGAGATTCAGGCCCCATACAACCTAAGGTGTGCCTTCAAGTCCGAGATCTTAGATGCCACAAAGCAAGCAGCGGAGCTGTTGCGCGGTTTGGCAAAAGATTTAAACAACATGAAGTGGAGCCTCCAAACTAGCTTACTTAAGCATGTTCATGTCTCGACCGAGCGTCTGCAACATTCAATTGACTTGCACTCCTACCTGTTCACCGCAAGTCAGGAAGACAACTATGCCAAACCACAATTGAAAATCTCAAGGGTTGTGTCTTTCAAGAACCAGTCCGGTGAACCAGAAAGCAAGACAACTGAAACCAGAACACCAATGGCGATGGAAGTTGAATCCTACCATGAGATGATGAAAAGGCAGCAGAGGAAGCTGCATTCATGGCCTTCCAGGGAGGTGGATGATTTTGAAGATGACGAAAATGTTGTTTCTGATCTCATTCCAAGGATGCGTGCACTTGAAAGCACCACAGCGCTGTCACTTGCAACTTTCACATCACTACTGATTGAATTTGTTGCTAGGCTTGATCATTTGGTTGAGGCTGCTGAGAGGCTTGCTACGATGGCAAGGTTCAAGCAGCAGATTGCAAACTAACCATTCAATGGCACTAACAGATTAAATACTAGCATAGTTACAGGATACAGAACTAAGTGCATACTATCTACCCAAAGCATCAAGTGATGTAACATTGTTGTTTGCTCTTTCTTGTACTAGAAAGGGGGAAAGTGTGTTTACATACTGGTATTTGCCAGTGGAGATATAAAATGTAATAGAAATTATACATGTTAACCTAATCCTGTGTGATCTGTCTAGCAAGGGGTGTAATTCCCATACATGACCAATCTTTTTAGAACAA
CDS Sequence
  • >LOC_Os01g12210.1
    ATGAATGGAAAGAAGGGTAGTATAAGGATTGACATTTGCCTGAAACCAAAAGATGTGGTGCCAGAAGATGCAAAGAAGCCAATCAGTGAAGAAGGGCTTTCCCCAAGGAAATGGCTACATGATGTCTGGGACTTCGCCAGACAGGATACAAACAGGGTCACCTTTGCACTCAAGGTTGGCCTTGCTTGCCTCCTTGTGTCACTGCTCATACTCTTCCGTGCACCCTATGACATCTTTGGGGCTAACATCATCTGGTCCATCCTCACTGTTGCTATAATGTTCGAGTACACTGTTGGTGCAACATTCAATCGCGGATTCAATCGAGCCGTCGGAAGTGTATTTGCTGGAGTATTTGCAGTAGTTGTTATTCAGGTGGCTATGTCTAGTGGCCATATTGCAGAACCATACATCATTGGTTTCAGCATATTTCTCATTGGTGCTGTAACATCATTTATGAAGCTATGGCCATCACTAGTTCCATATGAATATGGTTTTCGAGTTATCCTATTCACATATTGCTTGATCATCGTATCGGGCTACCGCATGGGAAATCCCATCAGGACTGCAATGGACAGACTCTACTCAATTGCGATCGGAGCCCTCATAGCCGTTTTAGTAAATGTCTTTATCTGCCCAATATGGGCAGGGGAGCAACTGCACAGGGAGCTAGTTAACAGCTTCAACTCTCTCGCAGACTCCTTAGAAGAGTGTGTCAAGAAGTATCTAAGTGATGATGGTTCCGAACATCCAGAGTTCTCAAAGACAGTCATGGATAACTTCCCTGATGAACCAGCATTCAGGAAGTGCCGAGCTACGTTAAATTCGTCAGCGAAGTTTGACTCTCTGGCGAACTCTGCTAAATGGGAACCACCACATGGGAGATTCAAGCACTTCTTTTACCCATGGGCAGAGTATGTGAAAGTTGGCAATGTTCTTCGCCACTGTGCCTACGAGGTGATGGCTTTGCATGGTTGCGTGCACTCAGAGATTCAGGCCCCATACAACCTAAGGTGTGCCTTCAAGTCCGAGATCTTAGATGCCACAAAGCAAGCAGCGGAGCTGTTGCGCGGTTTGGCAAAAGATTTAAACAACATGAAGTGGAGCCTCCAAACTAGCTTACTTAAGCATGTTCATGTCTCGACCGAGCGTCTGCAACATTCAATTGACTTGCACTCCTACCTGTTCACCGCAAGTCAGGAAGACAACTATGCCAAACCACAATTGAAAATCTCAAGGGTTGTGTCTTTCAAGAACCAGTCCGGTGAACCAGAAAGCAAGACAACTGAAACCAGAACACCAATGGCGATGGAAGTTGAATCCTACCATGAGATGATGAAAAGGCAGCAGAGGAAGCTGCATTCATGGCCTTCCAGGGAGGTGGATGATTTTGAAGATGACGAAAATGTTGTTTCTGATCTCATTCCAAGGATGCGTGCACTTGAAAGCACCACAGCGCTGTCACTTGCAACTTTCACATCACTACTGATTGAATTTGTTGCTAGGCTTGATCATTTGGTTGAGGCTGCTGAGAGGCTTGCTACGATGGCAAGGTTCAAGCAGCAGATTGCAAACTAA
Protein Sequence
  • >LOC_Os01g12210.1
    MNGKKGSIRIDICLKPKDVVPEDAKKPISEEGLSPRKWLHDVWDFARQDTNRVTFALKVGLACLLVSLLILFRAPYDIFGANIIWSILTVAIMFEYTVGATFNRGFNRAVGSVFAGVFAVVVIQVAMSSGHIAEPYIIGFSIFLIGAVTSFMKLWPSLVPYEYGFRVILFTYCLIIVSGYRMGNPIRTAMDRLYSIAIGALIAVLVNVFICPIWAGEQLHRELVNSFNSLADSLEECVKKYLSDDGSEHPEFSKTVMDNFPDEPAFRKCRATLNSSAKFDSLANSAKWEPPHGRFKHFFYPWAEYVKVGNVLRHCAYEVMALHGCVHSEIQAPYNLRCAFKSEILDATKQAAELLRGLAKDLNNMKWSLQTSLLKHVHVSTERLQHSIDLHSYLFTASQEDNYAKPQLKISRVVSFKNQSGEPESKTTETRTPMAMEVESYHEMMKRQQRKLHSWPSREVDDFEDDENVVSDLIPRMRALESTTALSLATFTSLLIEFVARLDHLVEAAERLATMARFKQQIAN*