Gene Details:

Functional Descriptions:

  • Here we report the characterization of two inward rectifying shaker-like potassium channels, OsKAT2 and OsKAT3, expressed in guard cell of rice plants.
  • While OsKAT2 showed typical potassium channel activity, like that of Arabidopsis KAT1, OsKAT3 did not despite high sequence similarity between the two channel proteins.
  • Interestingly, the two potassium channels physically interacted with each other and such interaction negatively regulated the OsKAT2 channel activity in CHO cell system.
  • Unique features of two potassium channels, OsKAT2 and OsKAT3, expressed in rice guard cells.
  • Interestingly, overexpression of OsKAT2(T235R) did not cause serious defects in growth or yield in rice, suggesting that OsKAT2 is a potential target for engineering plants with improved drought tolerance without yield penalty.
  • A Dominant Negative OsKAT2 Mutant Delays Light-Induced Stomatal Opening and Improves Drought Tolerance without Yield Penalty in Rice.
  • Our results indicate that OsKAT2 is an inward- rectifying shaker-like potassium channel that mainly functions in stomatal opening.
  • Here, we investigated the functions of OsKAT2 in stomatal regulation using three dominant negative mutant proteins, OsKAT2(T235R), OsKAT2(T285A) and OsKAT2(T285D), which are altered in amino acids in the channel pore and at a phosphorylation site.
  • In the present study, we found that the repression of OsAKTs, OsKAT2, and OsKAT2 in roots was related to salinity tolerance in rice.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os01g11250.1
    TTATTTATGCTGCATTCACAGCTACCACTTCACACTCAGCTAGTCAAGATTACCTGGCACACTCAGAAATGTACCACCAACATGTCAGTAATAAAAACAGCCTCTAGGAACTAAGATCTCGACTTAGGCACCTCCCACTTCTAACTAGACACTCCAATGGAAACTATTAGCAATATCTTTCACAATGACCCACTCCCACCTCTAGGAGCAAGAGCAAACCAATCTATCAAGCTGAGAAAATTCATTATTTCTCCATATGATTCCCGTTACAGGACCTGGGAGACGTTCTTGCTCGTGCTTGTTGTTTATTCAGCTTGGATCTGCCCATTTGAACTAGCATATCTGAGAAATCTCTCCTGGAAGGTTTCTCTTGTTGACAATATTATTGACAGCTTCTTTGCAATCGATATTATTCTCACATTTTTTCTAGCGTATCTGGATCAAAAGTCATATCTTCTAGTGGATGATCCAAAAAGGATCGTGGCTAGGTTACTTAGCATGCTTCGATTATGGCGTCTCCGGCGCCTCAGTGAATTATTTGCCAGGCTCGAAAAGGATATCCGATTGAATTACTACTGGATAAGGTGCACCAAACTAATTTCTGTTACACTTTTTGCGGTACATTGTTCAGGATGCTTCAATTATTTAATAGCTGATAGATACCCCAATCCAGCAAGAACATGGATAGGAGCAGCAATACCAAACTACAGATCACAAAATCTGTGGGTTCGATATGTTACGGCAATTTATTGGTCCATAACAACACTCACAACAACTGGTTATGGGGATTTGCATGCAGAGAATCAAAGAGAAATGTTATTTAGCATATGCTACATGCTATTTAACCTGGGATTGACAGCATACCTCATAGGTAACATGACAAATCTGGTTGTTCAGGGAAGTTGCCGGACCAGGAATTTTAGGGACACAATCCATGCTGCCTCTCAGTTTGCTGCAAGAAATCAGTTGCCTGGACATATTAAGGACGAAATGCTATCTCATATCTGCCTAAGATATAAAACAGAGGGGCTGAAACAGAAAGAAACATTGGATAGTCTCCCCAAGGGAATCCGTTCAAGCATAGCGTGCAACTTATTCTTACCTGTTATAGAAAAAGTCTATCTTTTCCATGGTGTCTCATTTACTTGCATGATCCAACTGGTAACTGAAATGGAAGCAGAGTACTACCCACCAAGAGAAGTTGTCATACTCCAAAATGAAGCACCCCGAGATGTTTACATATTAGTTTCGGGAGCAGTGGAAGAGCGGGTAGAGATCGATGGAACAGAAAAGGTCCAGGAAGTACTGTGCAATGGTGAAATATTTGGAGAAATAGGAGTTATCTGCAGCATCCCCCAGCCATGTGCTTTCCATACAATCAAAGTTTCACAACTATTAAGGCTGAACACAGCAGTGCTAAAGAACATTATCAAGGAAAACAGCGATGACAGACGAGTTATTCTGAACAATCTTTCCCAGAAGATGAACCAAGATCACAGGTTCTCTACCGAGGTGATGGAAAAATCTTTACAGATGATGCATCAACACTTTGGAGAGTACAACAGATGTTCTGCATTGAATCAAGACAATGAGAAGAATGAATTAAAAGCTAATAATGGTCATTCTATGGCTTTAGAATGGAAAAGAGTGACAATACATATGTATTCACAGAGGAACAAAAGACCGGAGGCACCGCTTGCAAAAGTTATAAATCTACCAGGATCTCTTGATAAACTTTTCGCCATAGCTTGTCAGAAGTTTAACAATTATCGTCTTACAAAACTTGTCAATCCAGAATTTGCTGAAATTGATGATATTACAGTAATTCGAGATGGTGATCATCTGTTTTTTATGGAAATTTGAATAAGGATGCTTGAAATCAGCAACGATGCAAAGAACTGCAGATAGGTATGTAGTATACCTTTCAGAAGGTACCAGATTAAGATAATAGTGATCACTAATCAGGCATGAGGCGACTTGGCATGATAATCAAGAACATGCCACCTAGGGATGTTTCGAGTTGCAGTCCTGTAGTTACATCTAATTTTACTTTAGAATGTAAATGTAGAATTTTGGAGCAATGTAGTCTCTATTCCGCTTTCAAAATGTTGGATCATGGCAGAGGGCAGCCCTCTATGATCTGTGGAGTTTGTAATGTAGCTATAGTGCAACGAAAGATACTAATATCGTTTACAGGTACTTTATAGTGTTTCTCCAAGTTTTTTTTGAAATAAATGATGAAACAGACATGC
CDS Sequence
  • >LOC_Os01g11250.1
    ATGGAAACTATTAGCAATATCTTTCACAATGACCCACTCCCACCTCTAGGAGCAAGAGCAAACCAATCTATCAAGCTGAGAAAATTCATTATTTCTCCATATGATTCCCGTTACAGGACCTGGGAGACGTTCTTGCTCGTGCTTGTTGTTTATTCAGCTTGGATCTGCCCATTTGAACTAGCATATCTGAGAAATCTCTCCTGGAAGGTTTCTCTTGTTGACAATATTATTGACAGCTTCTTTGCAATCGATATTATTCTCACATTTTTTCTAGCGTATCTGGATCAAAAGTCATATCTTCTAGTGGATGATCCAAAAAGGATCGTGGCTAGGTTACTTAGCATGCTTCGATTATGGCGTCTCCGGCGCCTCAGTGAATTATTTGCCAGGCTCGAAAAGGATATCCGATTGAATTACTACTGGATAAGGTGCACCAAACTAATTTCTGTTACACTTTTTGCGGTACATTGTTCAGGATGCTTCAATTATTTAATAGCTGATAGATACCCCAATCCAGCAAGAACATGGATAGGAGCAGCAATACCAAACTACAGATCACAAAATCTGTGGGTTCGATATGTTACGGCAATTTATTGGTCCATAACAACACTCACAACAACTGGTTATGGGGATTTGCATGCAGAGAATCAAAGAGAAATGTTATTTAGCATATGCTACATGCTATTTAACCTGGGATTGACAGCATACCTCATAGGTAACATGACAAATCTGGTTGTTCAGGGAAGTTGCCGGACCAGGAATTTTAGGGACACAATCCATGCTGCCTCTCAGTTTGCTGCAAGAAATCAGTTGCCTGGACATATTAAGGACGAAATGCTATCTCATATCTGCCTAAGATATAAAACAGAGGGGCTGAAACAGAAAGAAACATTGGATAGTCTCCCCAAGGGAATCCGTTCAAGCATAGCGTGCAACTTATTCTTACCTGTTATAGAAAAAGTCTATCTTTTCCATGGTGTCTCATTTACTTGCATGATCCAACTGGTAACTGAAATGGAAGCAGAGTACTACCCACCAAGAGAAGTTGTCATACTCCAAAATGAAGCACCCCGAGATGTTTACATATTAGTTTCGGGAGCAGTGGAAGAGCGGGTAGAGATCGATGGAACAGAAAAGGTCCAGGAAGTACTGTGCAATGGTGAAATATTTGGAGAAATAGGAGTTATCTGCAGCATCCCCCAGCCATGTGCTTTCCATACAATCAAAGTTTCACAACTATTAAGGCTGAACACAGCAGTGCTAAAGAACATTATCAAGGAAAACAGCGATGACAGACGAGTTATTCTGAACAATCTTTCCCAGAAGATGAACCAAGATCACAGGTTCTCTACCGAGGTGATGGAAAAATCTTTACAGATGATGCATCAACACTTTGGAGAGTACAACAGATGTTCTGCATTGAATCAAGACAATGAGAAGAATGAATTAAAAGCTAATAATGGTCATTCTATGGCTTTAGAATGGAAAAGAGTGACAATACATATGTATTCACAGAGGAACAAAAGACCGGAGGCACCGCTTGCAAAAGTTATAAATCTACCAGGATCTCTTGATAAACTTTTCGCCATAGCTTGTCAGAAGTTTAACAATTATCGTCTTACAAAACTTGTCAATCCAGAATTTGCTGAAATTGATGATATTACAGTAATTCGAGATGGTGATCATCTGTTTTTTATGGAAATTTGA
Protein Sequence
  • >LOC_Os01g11250.1
    METISNIFHNDPLPPLGARANQSIKLRKFIISPYDSRYRTWETFLLVLVVYSAWICPFELAYLRNLSWKVSLVDNIIDSFFAIDIILTFFLAYLDQKSYLLVDDPKRIVARLLSMLRLWRLRRLSELFARLEKDIRLNYYWIRCTKLISVTLFAVHCSGCFNYLIADRYPNPARTWIGAAIPNYRSQNLWVRYVTAIYWSITTLTTTGYGDLHAENQREMLFSICYMLFNLGLTAYLIGNMTNLVVQGSCRTRNFRDTIHAASQFAARNQLPGHIKDEMLSHICLRYKTEGLKQKETLDSLPKGIRSSIACNLFLPVIEKVYLFHGVSFTCMIQLVTEMEAEYYPPREVVILQNEAPRDVYILVSGAVEERVEIDGTEKVQEVLCNGEIFGEIGVICSIPQPCAFHTIKVSQLLRLNTAVLKNIIKENSDDRRVILNNLSQKMNQDHRFSTEVMEKSLQMMHQHFGEYNRCSALNQDNEKNELKANNGHSMALEWKRVTIHMYSQRNKRPEAPLAKVINLPGSLDKLFAIACQKFNNYRLTKLVNPEFAEIDDITVIRDGDHLFFMEI*