Gene Details:

• MSU gene ID: LOC_Os01g10504
• RAPdb gene ID: Os01g0201700
• Gene Symbol: OSMADS3
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• A knockout line of OSMADS3, in which the gene is disrupted by T-DNA insertion, shows homeotic transformation of stamens into lodicules and ectopic development of lodicules in the second whorl near the palea where lodicules do not form in the wild type but carpels develop almost normally.
• osmads13-3 OSMADS3-4 displayed a loss of floral meristem determinacy and generated abundant carpelloid structures containing severe defective ovules in the flower center, which were not detectable in the single mutant.
• Collectively, we propose a model to illustrate the role of OSMADS3, DL, and OsMADS13 in the specification of flower organ identity and meristem determinacy in rice.
• Here, we report the interactions of rice (Oryza sativa) floral homeotic genes, OSMADS3 (a C-class gene), OsMADS13 (a D-class gene), and DROOPING LEAF (DL), in specifying floral organ identities and floral meristem determinacy.
• This indicates that OSMADS3 plays a synergistic role with OsMADS13 in both ovule development and floral meristem termination.
• Strikingly, OSMADS3-4 dl-sup6 displayed a severe loss of floral meristem determinacy and produced supernumerary whorls of lodicule-like organs at the forth whorl, suggesting that OSMADS3 and DL synergistically terminate the floral meristem.
• Genetic interaction of OSMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy.
• As a consequence of the ectopic expression of the OSMADS3, lodicules were homeotically transformed into stamens.
• Ectopic Expression of OSMADS3, a Rice Ortholog of AGAMOUS, Caused a Homeotic Transformation of Lodicules to Stamens in Transgenic Rice Plants.
• In order to clarify the evolutionary relationship of floral organs between grasses and dicots, we expressed OSMADS3, a rice (Oryza sativa L.
• Loss of function of OSMADS3 via the insertion of a novel retrotransposon leads to recessive male sterility in rice (Oryza sativa).
• Genetic Enhancer Analysis Reveals that FLORAL ORGAN NUMBER2 and OSMADS3 Cooperatively Regulate Maintenance and Determinacy of the Flower Meristem in Rice.
• Disruption of OSMADS3 in the fon2 mutant by CRISPR-Cas9 technology caused a flower phenotype similar to that of 2B-424, confirming that the gene responsible for enhancement of fon2 was OSMADS3.
• These findings suggest that OSMADS3 is involved not only in FM determinacy in late flower development but also in FM activity in early flower development.
• Morphological analysis showed that the fon2 and OSMADS3 mutations synergistically affected pistil development and FM determinacy.
• Furthermore, our results indicate important roles of OSMADS32 in defining stamen, pistil and ovule development through physical and genetic interactions with OsMADS1, OsMADS58 and OsMADS13, and in specifying floral meristem identity with OsMADS6, OSMADS3 and OsMADS58 respectively.

Function-related keywords:

• palea ,  flower ,  meristem ,  stamen ,  leaf ,  floral-meristem ,  floral ,  sterility ,  male-sterility ,  development ,  floral-organ ,  flower-development ,  floral-organ-number ,  ovule

Literature:

• MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress .  DOI:  10.1186/1471-2164-8-242 ;  PMID:  17640358
• Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy .  DOI:  10.1104/pp.111.172080 ;  PMID:  21444646
• Functional analysis of all AGAMOUS subfamily members in rice reveals their roles in reproductive organ identity determination and meristem determinacy .  DOI:  10.1105/tpc.111.087007 ;  PMID:  21810995
• Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa .  DOI:  10.1105/tpc.105.037200 ;  PMID:  16326928
• Rice MADS3 regulates ROS homeostasis during late anther development .  DOI:  10.1105/tpc.110.074369 ;  PMID:  21297036
• Rice MADS6 interacts with the floral homeotic genes SUPERWOMAN1, MADS3, MADS58, MADS13, and DROOPING LEAF in specifying floral organ identities and meristem fate .  DOI:  10.1105/tpc.111.087262 ;  PMID:  21784949
• Ectopic expression of OsMADS3, a rice ortholog of AGAMOUS, caused a homeotic transformation of lodicules to stamens in transgenic rice plants .  DOI:  10.1093/pcp/pcf010 ;  PMID:  11828031
• Loss of function of OsMADS3 via the insertion of a novel retrotransposon leads to recessive male sterility in rice (Oryza sativa) .  DOI:  10.1016/j.plantsci.2015.06.007 ;  PMID:  26259187
• Genetic Enhancer Analysis Reveals that FLORAL ORGAN NUMBER2 and OsMADS3 Co-operatively Regulate Maintenance and Determinacy of the Flower Meristem in Rice .  DOI:  10.1093/pcp/pcx038 ;  PMID:  28371923
• Identification of a new function of metallothionein-like gene OsMT1e for cadmium detoxification and potential phytoremediation .  DOI:  10.1016/j.chemosphere.2020.129136 ;  PMID:  33276998
• Rice transcription factor MADS32 regulates floral patterning through interactions with multiple floral homeotic genes .  DOI:  10.1093/jxb/eraa588 ;  PMID:  33337484

Related News:

Gene Resources:

• NCBI ID: AK108568  , L37528
• UniProt accessions:

Sequences: