Information report for OsWRKY10
Gene Details
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Functional Descriptions
- Assays of disease in OsWRKY10KD and OsWRKY88KD lines following infection with an incompatible Xoo race, which induced Xa1-mediated resistance in wild-type plants, showed that OsWRKY10 and OsWRKY88 were positive regulators of Xa1-mediated resistance.
- Two transcriptional regulatory cascades of OsWRKY10 were identified in basal defense and Xa1-mediated resistance.
- These OsWRKY10 transcriptional regulatory cascades played important roles in basal defense and Xa1-mediated resistance to enable the mounting of a rapid immune response against pathogens.
- OsWRKY10 also acted as a positive regulator in basal defense by directly or indirectly activating transcription of defense-related genes.
- In the first transcriptional regulatory cascade, OsWRKY47 acted downstream of OsWRKY10 whereas OsWRKY51 acted upstream.
- The transcription factor OsWRKY10 inhibits phosphate uptake via suppressing OsPHT1;2 expression under phosphate-replete condition in rice.
- Here, we functionally characterized a transcription factor in rice (Oryza sativa), OsWRKY10.
- In accordance with this phenotype, OsWRKY10 was transcriptionally induced by Pi, and a subset of Phosphate Transporter 1 (PHT1) genes were up-regulated upon its mutation, suggesting that OsWRKY10 is a transcriptional repressor in Pi uptake.
- Moreover, rice plants expressing the OsWRKY10-VP16 fusion protein (a dominant transcriptional activator) accumulated even more Pi than OsWRKY10.
- Knockout of OsWRKY10 led to increased Pi uptake and accumulation under Pi-replete condition.
- Genetic analysis showed that OsPHT1;2 was responsible for the increased Pi accumulation in OsWRKY10.
- Furthermore, during Pi starvation, OsWRKY10 protein was degraded through the 26S proteasome.
Functional Keywords
Literature and News
- WRKY10 transcriptional regulatory cascades in rice are involved in basal defense and Xa1-mediated resistance . DOI: 10.1093/jxb/eraa135 ; PMID: 32227093
- The transcription factor OsWRKY10 inhibits phosphate uptake via suppressing OsPHT1;2 expression under phosphate-replete conditions in rice . DOI: 10.1093/jxb/erac456 ; PMID: 36402551
Gene Resources
- UniProt: Q7XAA3
- EMBL: AY341854, HQ858826
- AlphaFoldDB: Q7XAA3
- EnsemblPlants: Os01t0186000-01
- Gramene: Os01t0186000-01
- KEGG: osa:4325658
- InterPro: IPR003657, IPR036576, IPR044810
- PANTHER: PTHR31221, PTHR31221:SF377
- SUPFAM: SSF118290
- PROSITE: PS50811
- Gene3D: 2.20.25.80
- OrthoDB: Q7XAA3
- SWISS-MODEL: Q7XAA3
Sequences
cDNA Sequence
- >LOC_Os01g09100.1
CTGTCAAACACTCAATCAGCAGCAGCAGCAGCTAGCTTAGCTAACGCACAGTGACGCGAGCATGGCGGCTTCGCTGGGACTCTGCCACGAGACGTCGTACGCCTACTCGTACCCGGCGTCGAACACCTCCTCCTCCTTGTGCTTCCCTCCTCTCATGGCGGACCACATCGTCGACGGCGGCGGCGGCGGCGGCTGTTCCTTTGGTGAATTCCTGGAGCTCGGCCATTCCGTGTACTCGCTGCCGCTTCCGCCGCCGCCGTCGCAGCCGGTGGTGGTCGCCGGCGGGAACAATGATCAGTATGGCGTGTCGTCGTCGTCGTCGGCGGCGGCGACGACGAGCAGGATCGGGTTCAGGACGAGGTCGGAGGTGGAGGTGCTGGACGACGGGTTCAAGTGGCGAAAGTACGGGAAGAAGGCGGTGAAGAGCAGCCCGAACCCGCGGAACTACTACCGGTGCTCGGCGGCGGGGTGCGGCGTCAAGAAGCGGGTGGAGCGCGACGGCGACGACCCGCGCTACGTCGTCACCACCTACGACGGCGTCCACAACCACGCGACCCCCGGCTGCGTCGGCGGCGGCGGCCACCTCCCGTACCCTACTTCCGCTGCGCCGCCGTGGAGCGTTCCGGCGGCGGCGGCCTCGCCGCCTCCCGCGCACGCGCAGGCGTGGGGGGCGCCGCTGCACGCCGCCGCGGCGGCGCACTCGTCGGAATCGTCGTTCTGAACGCTGAATTTACTTCTGTTTTTGTTTTTCTTGGTTGCTTGTTAGTTGTACCGCCGTTCGTGATCGATCGATCGGTTCTTGTATGCACTAGACTTACTACTTAAAAAAAAAAGAGTTAAATCTTAACTATAAATCTAGACAATCATATGTTTAGAACTTCAGATTCGTCGTTAGAATTAATTTTTTTCTAAACGGAGTACGCCGTATTGTTTTAGCTATAAAATCGTGATGATTTGTGCAT
CDS Sequence
- >LOC_Os01g09100.1
ATGGCGGCTTCGCTGGGACTCTGCCACGAGACGTCGTACGCCTACTCGTACCCGGCGTCGAACACCTCCTCCTCCTTGTGCTTCCCTCCTCTCATGGCGGACCACATCGTCGACGGCGGCGGCGGCGGCGGCTGTTCCTTTGGTGAATTCCTGGAGCTCGGCCATTCCGTGTACTCGCTGCCGCTTCCGCCGCCGCCGTCGCAGCCGGTGGTGGTCGCCGGCGGGAACAATGATCAGTATGGCGTGTCGTCGTCGTCGTCGGCGGCGGCGACGACGAGCAGGATCGGGTTCAGGACGAGGTCGGAGGTGGAGGTGCTGGACGACGGGTTCAAGTGGCGAAAGTACGGGAAGAAGGCGGTGAAGAGCAGCCCGAACCCGCGGAACTACTACCGGTGCTCGGCGGCGGGGTGCGGCGTCAAGAAGCGGGTGGAGCGCGACGGCGACGACCCGCGCTACGTCGTCACCACCTACGACGGCGTCCACAACCACGCGACCCCCGGCTGCGTCGGCGGCGGCGGCCACCTCCCGTACCCTACTTCCGCTGCGCCGCCGTGGAGCGTTCCGGCGGCGGCGGCCTCGCCGCCTCCCGCGCACGCGCAGGCGTGGGGGGCGCCGCTGCACGCCGCCGCGGCGGCGCACTCGTCGGAATCGTCGTTCTGA
Protein Sequence
- >LOC_Os01g09100.1
MAASLGLCHETSYAYSYPASNTSSSLCFPPLMADHIVDGGGGGGCSFGEFLELGHSVYSLPLPPPPSQPVVVAGGNNDQYGVSSSSSAAATTSRIGFRTRSEVEVLDDGFKWRKYGKKAVKSSPNPRNYYRCSAAGCGVKKRVERDGDDPRYVVTTYDGVHNHATPGCVGGGGHLPYPTSAAPPWSVPAAAASPPPAHAQAWGAPLHAAAAAHSSESSF*