Gene Details:

• MSU gene ID: LOC_Os01g06740
• RAPdb gene ID: Os01g0160800
• Gene Symbol: OsRIP1 OsjRIP1.1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• The data obtained in this research indicate that OsRIP1 can play a role in plant defense against herbivorous insects.
• OsRIP1 inactivated insect ribosomes in vitro, suggesting that its toxicity relates to the enzymatic activity of OsRIP1.
• Furthermore, recombinant OsRIP1 was toxic for brown planthoppers when administered through liquid artificial diet.
• Over-expression of OsRIP1 in transgenic rice plants did not affect the performance of insects reared on these plants, most likely due to insufficient concentrations of OsRIP1 in the phloem.
• OsRIP1 provoked HeLa cells to undergo caspase-independent cell death, associated with a significant transcriptional upregulation of the apoptotic gene PUMA, interferon regulatory factor 1 (IRF1) and the autophagy-related marker LC3.
• Human cervical cancer HeLa cells were incubated in the presence of OsRIP1 for 24-72 h OsRIP1 treatment yielded an anti-proliferation response of the HeLa cells and resulted in apoptotic-like blebbing of the plasma membrane without causing DNA fragmentation.
• Our study provides the first evidence that OsRIP1 exerts differential effects on the growth of PSB-D and BY-2 cells.
• OsRIP1 triggered cell death in tobacco BY-2 cells but not in Arabidopsis PSB-D cells.
• Targeting of exogenous OsRIP1 to plant vacuoles and OsRIP1-induced accumulation of transcripts for vacuolar processing enzymes (VPEs) indicated that OsRIP1 provoked plant cell death in tobacco BY-2 cells through the activation of VPEs and subsequent vacuolar disruption, which was probably independent of its N-glycosylase activity on cytosolic ribosomes.
• The vacuole-dependent cell death pathway is associated with the lethal effect of the exogenously applied OsRIP1 on BY-2 cells.

Function-related keywords:

• defense ,  insect ,  brown-planthopper ,  phloem ,  cell-death ,  plasma-membrane ,  growth

Literature:

• Involvement of OsRIP1, a ribosome-inactivating protein from rice, in plant defense against Nilaparvata lugens .  DOI:  10.1016/j.phytochem.2019.112190 ;  PMID:  31731237
• Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of Rice .  DOI:  10.3390/ijms22031434 ;  PMID:  33535383
• The type-1 ribosome-inactivating protein OsRIP1 triggers caspase-independent apoptotic-like death in HeLa cells .  DOI:  10.1016/j.fct.2021.112590 ;  PMID:  34601042
• Differential effects of the recombinant type 1 ribosome-inactivating protein, OsRIP1, on growth of PSB-D and BY-2 cells .  DOI:  10.3389/fpls.2022.1019591 ;  PMID:  36247583

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: