Information report for OsRIP1;OsjRIP1.1
Gene Details
|
|
Functional Descriptions
- The data obtained in this research indicate that OsRIP1 can play a role in plant defense against herbivorous insects.
- OsRIP1 inactivated insect ribosomes in vitro, suggesting that its toxicity relates to the enzymatic activity of OsRIP1.
- Furthermore, recombinant OsRIP1 was toxic for brown planthoppers when administered through liquid artificial diet.
- Over-expression of OsRIP1 in transgenic rice plants did not affect the performance of insects reared on these plants, most likely due to insufficient concentrations of OsRIP1 in the phloem.
- OsRIP1 provoked HeLa cells to undergo caspase-independent cell death, associated with a significant transcriptional upregulation of the apoptotic gene PUMA, interferon regulatory factor 1 (IRF1) and the autophagy-related marker LC3.
- Human cervical cancer HeLa cells were incubated in the presence of OsRIP1 for 24-72 h OsRIP1 treatment yielded an anti-proliferation response of the HeLa cells and resulted in apoptotic-like blebbing of the plasma membrane without causing DNA fragmentation.
- Our study provides the first evidence that OsRIP1 exerts differential effects on the growth of PSB-D and BY-2 cells.
- OsRIP1 triggered cell death in tobacco BY-2 cells but not in Arabidopsis PSB-D cells.
- Targeting of exogenous OsRIP1 to plant vacuoles and OsRIP1-induced accumulation of transcripts for vacuolar processing enzymes (VPEs) indicated that OsRIP1 provoked plant cell death in tobacco BY-2 cells through the activation of VPEs and subsequent vacuolar disruption, which was probably independent of its N-glycosylase activity on cytosolic ribosomes.
- The vacuole-dependent cell death pathway is associated with the lethal effect of the exogenously applied OsRIP1 on BY-2 cells.
Functional Keywords
- defense , insect , brown-planthopper , phloem , cell-death , plasma-membrane , growth
Literature and News
- Involvement of OsRIP1, a ribosome-inactivating protein from rice, in plant defense against Nilaparvata lugens . DOI: 10.1016/j.phytochem.2019.112190 ; PMID: 31731237
- Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of Rice . DOI: 10.3390/ijms22031434 ; PMID: 33535383
- The type-1 ribosome-inactivating protein OsRIP1 triggers caspase-independent apoptotic-like death in HeLa cells . DOI: 10.1016/j.fct.2021.112590 ; PMID: 34601042
- Differential effects of the recombinant type 1 ribosome-inactivating protein, OsRIP1, on growth of PSB-D and BY-2 cells . DOI: 10.3389/fpls.2022.1019591 ; PMID: 36247583
Gene Resources
- UniProt: Q9LGK6
- EMBL: AK103707, AP014957
- AlphaFoldDB: Q9LGK6
- EnsemblPlants: Os01t0160800-01
- Gramene: Os01t0160800-01
- KEGG: osa:4324068
- Orthologous matrix: NPRWLGF
- InterPro: IPR001574, IPR016138, IPR016139
- PANTHER: PTHR33453, PTHR33453:SF27
- SUPFAM: SSF56371
- Gene3D: 3.40.420.10, 4.10.470.10
- OrthoDB: Q9LGK6
- SWISS-MODEL: Q9LGK6
- eggNOG: ENOG502S89M
Sequences
cDNA Sequence
- >LOC_Os01g06740.1
GTGTCATCTTGTCTATGGTAGTTCAGCAATCCTCTGCTTTTGTCAGTTGTAGTTCACAACGATCGAGCATCCGGCCATGGCGTTGAACCCGCTGTTCACCGTGACGTTCAACGTGAGCGGCAGCGGCAGCGACAACTACGGCGACTTCATCGCCGGCATCCGCAAGAGGGTGGCCAACCCGAGGCACTTCTCCCACAACCGCCCCGTCCTCCCGCCGGTGGAGCCCGACGTCCCGCCGCGCCGCTGGTTCCACGTCGTGCTCCGGACGCAGACCAGCGAGCTCACGCTCGCCACGCGCGCCGACAACCTCTACCTGGAGGGCTTCCGCGGCAGCGACGGCACCAGCGCGTGGTGGGAGCTCACCCGCGGCCTCATCGCCGGCGCCACCTACCTCGGCTTCGGCGGCTCGTACCGCGAGCTCCTCGGGCACACGGACAACATGGTCGGTGTCACGCTCGGCCCGCAGCAGATGACGCAGGCCGTGGACACGCTCGCCGGTCTCGCCGCCAGCGGCGGCGGCGCGGCGCGGCAACGGGCCGGCGAGGCGTTGGCGACGCTGCTCCTGATGGTGAACGAAGCCGTGCGTTTCCTGACCGTGGCGGAGCTTGTGGGCGGCTTCATGAACCCCAGGGCGGTGAGGAAGAGCGGGACGATCACCGCCGACATGAAGGAGCAGGTGAACGGGTGGAAGGTCTTGTCCAGGGCGCTGCTCACCATGGACGCGCTGCAGCTAGAGGACTCCAACTCGGCGTCCAAGCACAACAAGGTGGATACAAAGAAGATGGAGCAGGAGAAGAAGGCGTGGGAGGCGGCGGAGAAGTTGGCCGTGGAGGCGGCCAAGGCGGTGGGGATCCTGCTGTTCGTCGAGAAGGTGCCGGCCGGGATGACGAAGGCGACGGCGCTTCAGCTGTTTCATGGGAACTAAAGTTGCTGCTGCTGGTAGATTTCAGCTTGCTTGATCGAACAGCAAATCAATCTGAGCGACTCGAGTAAAATTGATTCTCCCTCCAACCCAAAATATAAGTATTTTCAGTTATTAATCTGGACAACTGCGTGTCCAGATACATAGACAAAAGTTGTTATATTTTGAGACGGTTGTTATATTTTGGGACGGAGGCAGTAGTTAAATAGCCTCTATAGTGTGATGTAACTACTATATACTCCCTTCGTCCCAAAAAAGACAAACACTGGTCTCCGTGTCCAACGTTTGACTGTCCGTCTTATATGAAATTTTTTTATAATTAATATTTTCATTGTTGTTAGATGATAAAACATGATTAATAATTTATGTGTGACTTGTCTTTTTAATTTTTTCATAATTTTTTTAAATAGACGGACGGTCAAACGTTGACACGGAAACTCAGGGTTTGTCTTTTTTTTTAATGGAGGGAGTATGTATATGATGTGTGTATCGATCCTAGATTCCTACCTCTCCGTCCCAAAAAAAAATCCAATCCTAGCTACGAACCTAGACACACGCGTGTCCAGGTTCATAGCCAGAGTTTGGGTTTTTTTGGATAGACTAGTACACTGTAACTCAAAAATAAAGATAGCAGCAATGGTCAGTTTGCTCC
CDS Sequence
- >LOC_Os01g06740.1
ATGGCGTTGAACCCGCTGTTCACCGTGACGTTCAACGTGAGCGGCAGCGGCAGCGACAACTACGGCGACTTCATCGCCGGCATCCGCAAGAGGGTGGCCAACCCGAGGCACTTCTCCCACAACCGCCCCGTCCTCCCGCCGGTGGAGCCCGACGTCCCGCCGCGCCGCTGGTTCCACGTCGTGCTCCGGACGCAGACCAGCGAGCTCACGCTCGCCACGCGCGCCGACAACCTCTACCTGGAGGGCTTCCGCGGCAGCGACGGCACCAGCGCGTGGTGGGAGCTCACCCGCGGCCTCATCGCCGGCGCCACCTACCTCGGCTTCGGCGGCTCGTACCGCGAGCTCCTCGGGCACACGGACAACATGGTCGGTGTCACGCTCGGCCCGCAGCAGATGACGCAGGCCGTGGACACGCTCGCCGGTCTCGCCGCCAGCGGCGGCGGCGCGGCGCGGCAACGGGCCGGCGAGGCGTTGGCGACGCTGCTCCTGATGGTGAACGAAGCCGTGCGTTTCCTGACCGTGGCGGAGCTTGTGGGCGGCTTCATGAACCCCAGGGCGGTGAGGAAGAGCGGGACGATCACCGCCGACATGAAGGAGCAGGTGAACGGGTGGAAGGTCTTGTCCAGGGCGCTGCTCACCATGGACGCGCTGCAGCTAGAGGACTCCAACTCGGCGTCCAAGCACAACAAGGTGGATACAAAGAAGATGGAGCAGGAGAAGAAGGCGTGGGAGGCGGCGGAGAAGTTGGCCGTGGAGGCGGCCAAGGCGGTGGGGATCCTGCTGTTCGTCGAGAAGGTGCCGGCCGGGATGACGAAGGCGACGGCGCTTCAGCTGTTTCATGGGAACTAA
Protein Sequence
- >LOC_Os01g06740.1
MALNPLFTVTFNVSGSGSDNYGDFIAGIRKRVANPRHFSHNRPVLPPVEPDVPPRRWFHVVLRTQTSELTLATRADNLYLEGFRGSDGTSAWWELTRGLIAGATYLGFGGSYRELLGHTDNMVGVTLGPQQMTQAVDTLAGLAASGGGAARQRAGEALATLLLMVNEAVRFLTVAELVGGFMNPRAVRKSGTITADMKEQVNGWKVLSRALLTMDALQLEDSNSASKHNKVDTKKMEQEKKAWEAAEKLAVEAAKAVGILLFVEKVPAGMTKATALQLFHGN*