Gene Details:

• MSU gene ID: LOC_Os01g06640
• RAPdb gene ID: Os01g0159800
• Gene Symbol: OsbHLH035 OsbHLH35
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• The transcription factor OsbHLH035 mediates seed germination and enables seedling recovery from salt stress through ABA-dependent and ABA-independent pathways, respectively.
• The bHLH TF OsbHLH035 is a salt-induced gene that is primarily expressed in germinating seeds and seedlings.
• Moreover, OsbHLH035 mutant seedlings are unable to recover from salt-stress treatment.
• Consistently, sodium is over-accumulated in aerial tissues but slightly reduced in terrestrial tissues from OsbHLH035 seedlings after salt treatment.
• Furthermore, genetic complementation can restore both the delayed seed germination and the impaired recovery of salt-treated OsbHLH035 seedlings to normal growth.
• OsbHLH035 mediates seed germination and seedling recovery after salt stress relief through the ABA-dependent and ABA-independent activation of OsHKT pathways, respectively.
• OsbHLH035 mutants show delayed seed germination, particularly under salt-stress conditions.
• The expression of OsbHLH035 is induced by drought and salinity.
• In parallel, abscisic acid (ABA) contents are over-accumulated, and the expression of the ABA biosynthetic genes OsABA2 and OsAAO3 is upregulated; furthermore, compared with that in wild-type (WT) seedlings, the salt-induced expression of OsABA8ox1, an ABA catabolic gene, in germinating OsbHLH035 mutant seeds is downregulated.
• Stable expression of GFP-fused OsbHLH035 in rice transgenic plants revealed that this protein is predominantly localized to the nucleus.
• Tightly controlled expression of OsbHLH35 is critical for anther development in rice.
• Our data suggest that OsbHLH35 plays an essential role in anther development in rice and the fine control of its expression is crucial to ensure proper seed production.
• Rice transgenic plants expressing GUS reporter gene under the control of OsbHLH35 promoter (pOsbHLH35::GUS) showed that this TF specifically accumulates in anthers at the meiosis stage and in other spikelet tissues.
• Plants overexpressing OsbHLH35 presented small and curved anthers, leading to a reduction of 72 % on seed production.
• Yeast one-hybrid screening identified three members of the Growth-Regulating Factor (GRF) family, OsGRF3, OsGRF4, and OsGRF11, as transcriptional regulators of OsbHLH35.

Function-related keywords:

• transcription-factor ,  seedlings ,  growth ,  seedling ,  seed ,  drought ,  salinity ,  salt ,  seed-germination ,  ABA ,  salt-stress ,  stress ,  nucleus ,  abscisic-acid ,  development ,  spikelet ,  anther ,  meiosis ,  anther-development ,  transcriptional-regulator

Literature:

• The transcription factor OsbHLH035 mediates seed germination and enables seedling recovery from salt stress through ABA-dependent and ABA-independent pathways, respectively .  DOI:  10.1186/s12284-018-0244-z ;  PMID:  30203325
• Tightly controlled expression of OsbHLH35 is critical for anther development in rice .  DOI:  10.1016/j.plantsci.2020.110716 ;  PMID:  33288022

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: