Gene Details:

Functional Descriptions:

  • Refunctionalization of the ancient rice blast disease resistance gene Pit by the recruitment of a retrotransposon as a promoter.
  • We previously showed that the resistance function of the rice blast resistance gene Pit in a resistant cultivar, K59, was mainly acquired by up-regulated promoter activity through the insertion of a long terminal repeat (LTR) retrotransposon upstream of Pit.
  • Here, we report that a long terminal repeat (LTR) retrotransposon contributed to the evolution of the rice blast resistance gene Pit.
  • The sequence and expression pattern of Pit in the five cultivars were the same as those in K59 and all showed Pit-mediated blast resistance.
  • Identification of the blast resistance gene Pit in rice cultivars using functional markers.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os01g05620.1
    ATGGGCACAGTTTTGGATGCTCTCGCTTGGAAATTCTTGGAAAAGCTGGGTCAACTTATTGAGGATGAGGTTATCATGACATTAAGTGTGAAAAGGGGTATTGAGAGCCTGAAGAAAAATCTGGAATTCTTCAACGCTGTTCATGAGGATGCTGAGGCTCTGGCCATGGAGGATCCAGGGATAGATTCATGGTGGAAGAACATGAGAGATGTTATGTTTGATGTCGATGACATCGTAGATCTTTTCATGGTTCATTCACAGAAGCTCTTGCTGCCACCAAGGCCGGTATGTTGCAATCAGCCCTTGTTCTCTAGCTTTGCAAAGTTTTCATTTGATCACATGATTGCCAAAAGAATTGATAATATAAATGAAAAGTTTGAAGAAATTAAAATGAACAAAGAGATGTTTGGCTTGGAGAGAACCAACGGGCAACAAATCCAGATAACAATTGTTGACAGAAGTCAGACATCTCCTGTAGATGAACTTGAGGTGGTTGGAGAAGATATCAGAAGGGCTATTGATGACATCGTCAAAATGATTGTTAGTAGTAACTACAATGAAAGCAGGTCAACTGTTTTTGGGATCCAGGGGATGGGAGGCATCGGTAAGACAACATTAGCTCAAAAGATATACAATGAACAGAGGATAAGGGAGAAATTCCAAGTTCATATATGGTTGTGCATTTCTCAGAATTACACAGAGACCAGTTTGTTAAAACAGGCAATCAGAATGGCTGGAGGAATTTGTGATCAGCTGGAGACTAAGACTGAGCTTCTACCGCTTCTAGTGGACACTATTAGAGGGAAGAGTGTTTTTCTTGTGCTGGATGATGTTTGGAAATCTGATGTATGGATTGATCTTCTTCGTTTGCCTTTCTTGAGAGGTTTGAATTCTCATATCCTTGTGACCTCAAGAAACCTGGATGTTTTGGTTGAAATGCATGCAACTTATACCCACAAGGTAAACAAAATGAATGATTGCGATGGCCTAGAACTGCTGATGAAGATGTCATTAGGGCCATACGAGCAAAGCAGAGAATTCAGTGGTGTTGGATATCAAATTGTCAAAAAGTGTGATGGCCTTCCTCTTGCCATCAAAGTTGTTGCAGGTGTTCTATCTACTAAAAGAACAAGGGCAGAATGGGAGAGTATCCGAGATAGCAAGTGGTCCATTCATGGACTTCCCAGAGAACTAGGAGGCCCTCTGTACTTAAGCTACAGCAACTTACCCCCTGAACTTAAACAATGCTTTCTCTGGTGTGCTTTGTTGCCGTCAAATTTTGTGATACGTCGTGATGCTGTCGCTTACTGGTGGGTTGCTGAAGGTTTCGTGACGGAAGTGCATGGGTACTCAATACATGAGGTTGCTGAAGAGTACTACCATGAGCTAATCAGAAGGAATCTTCTGCAGCCACGACCAGAGTTTGTAGACAAAGGCGAGTCAACAATGCATGATCTATTGAGGTCACTTGGTCAATTTTTGACAAAGGATCACTCCATATTCATGAACATGGAATATAGCAAAGCCTTACCAAATCTACGCCACTTATGTATCAGCAATGATGTAGAAGAAATACCTGCTATAGAAAAGCAAAAGTGCTTGAGGAGCCTCCTAGTTTTTGATAACAAAAACTTCATGAAAATTAATAAGGACATTTTCAGAGAACTCAAGCATATCCGTGTCTTAGTTCTCAGTGGAACAAGCATCCAAATCATACCAGAGTCAGTGGGAAACTTCTTGTTACTGAGGCTTCTAGATCTAAGCTATACAAAAATTCAGAAACTCCCAGAATCCATAGGAAAACTTACCAGCCTTGAATACCTTTCTTTGCATGGTTGCATACATTTGGATAGCCTGCCAGATAGTTTAATGAGACTGTCGAACATAAGTTTCCTAGAGCTAGAACAGACTGCAATCGACCATGTTCCAAAAGGAGTTGCAAAGTTGCAGCAACTCTACAACCTTAGAGGAGTTTTTGACAGTGGAACTGGATTCAGACTAGATGAACTCCAATGTCTCTCCAATATCCAACGTCTCCGGATTGTGAAGCTAGAAAAAGCAGCACCAGGGGGTTCATTTGTACTCAAGAACTGTCTTCATCTTCGAGAATTGTGGCTCGGTTGCACAATCGGAGGTCATGATAAAACTTATTATCAGACCAACGAGATTGAGAGAATTCAGCAAGTCTACGAACTGCTAATTCCATCGCCAAGCTTACTGTATATATTTCTTGTCGGGTTCCCTGGTGTAAGGTTCCCAGATTGGTTGTGCTCAGAACCAGAGCGTAAGATGCCAAATTTGGGTCATATGCACCTCAACGACTGCACTTCATGTTCAGTGCTTCCACCAGCAGGCCAGATGCCAGAACTGCTAGTTTTTAAGATTAAAGGCGCAGATGCAATAGTGAATATGGGTGCAGAGCTGCTAGGGAAAGGCGTCAATAGCGCAAAGCATATAACTATTTTTCCAAAACTCGAATTGCTTCTCATCACTAACATGAGCAATTTGGAAAGTTGGTCTCTCAACACATGGAATTTGTGTGGCAAATCTGAACAGCTTGTTCTGATGCCTTGCCTTAAGCGTTTGTTTCTTAACGACTGCCCAAAACTAAGGGCTCTCCCTGAAGACTTGCATAGGATTGCTAACTTAAGAAGAATCCATATCGAAGGTGCTCACACGCTGCAAGAAGTTGACAACCTTCCTTCAGTGTTATGGCTCAAGGTCAAGAACAACAGGTGTTTGAGGAGAATTTCCAATCTCTGTAATCTAAAGGACTTGCTTGCGCAGGACTGTCCAGCGCTGTATCAGGCAGAGAATCTAATCTCACTAAAGAGACTCTACATGGTCGATTGCCACAACGCGAAGCAATTCAGGATGAGCCTCCTGGAAGACCAACAACTTGCCGTCCATGTTGTTACAGTTGGTGCAGATGGCAGGGATATCTTTCCAGATGAATCTCTCTATAATTAG
CDS Sequence
  • >LOC_Os01g05620.1
    ATGGGCACAGTTTTGGATGCTCTCGCTTGGAAATTCTTGGAAAAGCTGGGTCAACTTATTGAGGATGAGGTTATCATGACATTAAGTGTGAAAAGGGGTATTGAGAGCCTGAAGAAAAATCTGGAATTCTTCAACGCTGTTCATGAGGATGCTGAGGCTCTGGCCATGGAGGATCCAGGGATAGATTCATGGTGGAAGAACATGAGAGATGTTATGTTTGATGTCGATGACATCGTAGATCTTTTCATGGTTCATTCACAGAAGCTCTTGCTGCCACCAAGGCCGGTATGTTGCAATCAGCCCTTGTTCTCTAGCTTTGCAAAGTTTTCATTTGATCACATGATTGCCAAAAGAATTGATAATATAAATGAAAAGTTTGAAGAAATTAAAATGAACAAAGAGATGTTTGGCTTGGAGAGAACCAACGGGCAACAAATCCAGATAACAATTGTTGACAGAAGTCAGACATCTCCTGTAGATGAACTTGAGGTGGTTGGAGAAGATATCAGAAGGGCTATTGATGACATCGTCAAAATGATTGTTAGTAGTAACTACAATGAAAGCAGGTCAACTGTTTTTGGGATCCAGGGGATGGGAGGCATCGGTAAGACAACATTAGCTCAAAAGATATACAATGAACAGAGGATAAGGGAGAAATTCCAAGTTCATATATGGTTGTGCATTTCTCAGAATTACACAGAGACCAGTTTGTTAAAACAGGCAATCAGAATGGCTGGAGGAATTTGTGATCAGCTGGAGACTAAGACTGAGCTTCTACCGCTTCTAGTGGACACTATTAGAGGGAAGAGTGTTTTTCTTGTGCTGGATGATGTTTGGAAATCTGATGTATGGATTGATCTTCTTCGTTTGCCTTTCTTGAGAGGTTTGAATTCTCATATCCTTGTGACCTCAAGAAACCTGGATGTTTTGGTTGAAATGCATGCAACTTATACCCACAAGGTAAACAAAATGAATGATTGCGATGGCCTAGAACTGCTGATGAAGATGTCATTAGGGCCATACGAGCAAAGCAGAGAATTCAGTGGTGTTGGATATCAAATTGTCAAAAAGTGTGATGGCCTTCCTCTTGCCATCAAAGTTGTTGCAGGTGTTCTATCTACTAAAAGAACAAGGGCAGAATGGGAGAGTATCCGAGATAGCAAGTGGTCCATTCATGGACTTCCCAGAGAACTAGGAGGCCCTCTGTACTTAAGCTACAGCAACTTACCCCCTGAACTTAAACAATGCTTTCTCTGGTGTGCTTTGTTGCCGTCAAATTTTGTGATACGTCGTGATGCTGTCGCTTACTGGTGGGTTGCTGAAGGTTTCGTGACGGAAGTGCATGGGTACTCAATACATGAGGTTGCTGAAGAGTACTACCATGAGCTAATCAGAAGGAATCTTCTGCAGCCACGACCAGAGTTTGTAGACAAAGGCGAGTCAACAATGCATGATCTATTGAGGTCACTTGGTCAATTTTTGACAAAGGATCACTCCATATTCATGAACATGGAATATAGCAAAGCCTTACCAAATCTACGCCACTTATGTATCAGCAATGATGTAGAAGAAATACCTGCTATAGAAAAGCAAAAGTGCTTGAGGAGCCTCCTAGTTTTTGATAACAAAAACTTCATGAAAATTAATAAGGACATTTTCAGAGAACTCAAGCATATCCGTGTCTTAGTTCTCAGTGGAACAAGCATCCAAATCATACCAGAGTCAGTGGGAAACTTCTTGTTACTGAGGCTTCTAGATCTAAGCTATACAAAAATTCAGAAACTCCCAGAATCCATAGGAAAACTTACCAGCCTTGAATACCTTTCTTTGCATGGTTGCATACATTTGGATAGCCTGCCAGATAGTTTAATGAGACTGTCGAACATAAGTTTCCTAGAGCTAGAACAGACTGCAATCGACCATGTTCCAAAAGGAGTTGCAAAGTTGCAGCAACTCTACAACCTTAGAGGAGTTTTTGACAGTGGAACTGGATTCAGACTAGATGAACTCCAATGTCTCTCCAATATCCAACGTCTCCGGATTGTGAAGCTAGAAAAAGCAGCACCAGGGGGTTCATTTGTACTCAAGAACTGTCTTCATCTTCGAGAATTGTGGCTCGGTTGCACAATCGGAGGTCATGATAAAACTTATTATCAGACCAACGAGATTGAGAGAATTCAGCAAGTCTACGAACTGCTAATTCCATCGCCAAGCTTACTGTATATATTTCTTGTCGGGTTCCCTGGTGTAAGGTTCCCAGATTGGTTGTGCTCAGAACCAGAGCGTAAGATGCCAAATTTGGGTCATATGCACCTCAACGACTGCACTTCATGTTCAGTGCTTCCACCAGCAGGCCAGATGCCAGAACTGCTAGTTTTTAAGATTAAAGGCGCAGATGCAATAGTGAATATGGGTGCAGAGCTGCTAGGGAAAGGCGTCAATAGCGCAAAGCATATAACTATTTTTCCAAAACTCGAATTGCTTCTCATCACTAACATGAGCAATTTGGAAAGTTGGTCTCTCAACACATGGAATTTGTGTGGCAAATCTGAACAGCTTGTTCTGATGCCTTGCCTTAAGCGTTTGTTTCTTAACGACTGCCCAAAACTAAGGGCTCTCCCTGAAGACTTGCATAGGATTGCTAACTTAAGAAGAATCCATATCGAAGGTGCTCACACGCTGCAAGAAGTTGACAACCTTCCTTCAGTGTTATGGCTCAAGGTCAAGAACAACAGGTGTTTGAGGAGAATTTCCAATCTCTGTAATCTAAAGGACTTGCTTGCGCAGGACTGTCCAGCGCTGTATCAGGCAGAGAATCTAATCTCACTAAAGAGACTCTACATGGTCGATTGCCACAACGCGAAGCAATTCAGGATGAGCCTCCTGGAAGACCAACAACTTGCCGTCCATGTTGTTACAGTTGGTGCAGATGGCAGGGATATCTTTCCAGATGAATCTCTCTATAATTAG
Protein Sequence
  • >LOC_Os01g05620.1
    MGTVLDALAWKFLEKLGQLIEDEVIMTLSVKRGIESLKKNLEFFNAVHEDAEALAMEDPGIDSWWKNMRDVMFDVDDIVDLFMVHSQKLLLPPRPVCCNQPLFSSFAKFSFDHMIAKRIDNINEKFEEIKMNKEMFGLERTNGQQIQITIVDRSQTSPVDELEVVGEDIRRAIDDIVKMIVSSNYNESRSTVFGIQGMGGIGKTTLAQKIYNEQRIREKFQVHIWLCISQNYTETSLLKQAIRMAGGICDQLETKTELLPLLVDTIRGKSVFLVLDDVWKSDVWIDLLRLPFLRGLNSHILVTSRNLDVLVEMHATYTHKVNKMNDCDGLELLMKMSLGPYEQSREFSGVGYQIVKKCDGLPLAIKVVAGVLSTKRTRAEWESIRDSKWSIHGLPRELGGPLYLSYSNLPPELKQCFLWCALLPSNFVIRRDAVAYWWVAEGFVTEVHGYSIHEVAEEYYHELIRRNLLQPRPEFVDKGESTMHDLLRSLGQFLTKDHSIFMNMEYSKALPNLRHLCISNDVEEIPAIEKQKCLRSLLVFDNKNFMKINKDIFRELKHIRVLVLSGTSIQIIPESVGNFLLLRLLDLSYTKIQKLPESIGKLTSLEYLSLHGCIHLDSLPDSLMRLSNISFLELEQTAIDHVPKGVAKLQQLYNLRGVFDSGTGFRLDELQCLSNIQRLRIVKLEKAAPGGSFVLKNCLHLRELWLGCTIGGHDKTYYQTNEIERIQQVYELLIPSPSLLYIFLVGFPGVRFPDWLCSEPERKMPNLGHMHLNDCTSCSVLPPAGQMPELLVFKIKGADAIVNMGAELLGKGVNSAKHITIFPKLELLLITNMSNLESWSLNTWNLCGKSEQLVLMPCLKRLFLNDCPKLRALPEDLHRIANLRRIHIEGAHTLQEVDNLPSVLWLKVKNNRCLRRISNLCNLKDLLAQDCPALYQAENLISLKRLYMVDCHNAKQFRMSLLEDQQLAVHVVTVGADGRDIFPDESLYN*