Gene Details:
- MSU gene ID: LOC_Os01g03640
- RAPdb gene ID: Os01g0127200
- Gene Symbol: OsLPR5
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- qRT-PCR and promoter-GUS reporter analyses indicated that under Pi-sufficient conditions OsLPR5 was highly expressed in the roots, and specific expression occurred in the leaf collars and nodes, and its expression was increased under Pi-deficient conditions.
- Overexpression of OsLPR5 triggered higher ferroxidase activity, and elevated concentrations of Fe(III) in the xylem sap and of total Fe in the roots and shoots.
- Knockout mutation in OsLPR5 by means of CRISPR-Cas9 resulted in adverse effects on Pi translocation, on the relative expression of Cis-NATOsPHO1;2, and on several morphological traits, including root development and yield potential.
- Our results indicate that ferroxidase-dependent OsLPR5 has both a broad-spectrum influence on growth and development in rice as well as affecting a subset of physiological and molecular traits that govern Pi homeostasis.
- Transient expression of OsLPR5 in Nicotiana benthamiana provided evidence of its subcellular localization to the cell wall and endoplasmic reticulum.
Function-related keywords:
- leaf , xylem , root , growth , development , yield , root-development , homeostasis , cell-wall , Pi , Fe , pi , Pi-homeostasis
Literature:
- Identification and expression analysis of OsLPR family revealed the potential roles of OsLPR3 and 5 in maintaining phosphate homeostasis in rice . DOI: 10.1186/s12870-016-0853-x ; PMID: 27716044
- The ferroxidase LPR5 functions in the maintenance of phosphate homeostasis and is required for normal growth and development of rice . DOI: 10.1093/jxb/eraa211 ; PMID: 32618334
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os01g03640.1
ATCGATCAGTCGATCAGCTAATCTCTTTCTCCTTTAATTCATTATCCTTTGCATGCCCCTCTCCAGCTAGCTAAGATGAGTCCGAGGATTCAGCAGCTGGCCGCTGTGTTGTTGGCGGCTGTTGTCGTCGTCGCAGCCGCCAGGGATGAACCTGCAGCAGCAAAAAATTACCAAACTCAATGGGATACAGTAATGTCAATACTTAACTGTAAGAGTGATTCTTTAATCCCAAGCTACATCTGCAGCGTGATTAGCAAAAGTCGCTGGGGTTGGGCGTCAGATGACCCCAACGACGACGAGTACACACCACCGGATCATCCGCTGCCGGCGCCGGCGGCGGGGCGGCGGCGGTGGCCGGTGATGACGAGCTTGAACCTGACAAAGTACGTGGACTCGCTGCCGCGGATTGCCAAGATCCGGGGCTACGGCATCCGCCATGGCCGTCCAGTGCCCATCAAGCTCACCATCGGCATGTACAGCAAGACATGGCAATTCCACCGCGACATGCCACCGACGCCGGTGTTCGTGTACGGGCAATCGCTGCAGACAGCGACGTTCCCGGGGCCCACCATCGTGGCGCGGCAGGGCGTGCCGCTCGCCGTGGAGTGGCAGAACCACCTCCCCGACGCCCACATCCTCCCGTGGGACCCCAAGGTCCCCACCGCCATCCCCAAGAAGGGCGGCGTCCCCACCGTCGTTCACCTCCACGGCGGCGCCCACCCGCCGGAGTTCGACGGCCACGCCTTCGCCTGGTTCACCCGCGACTTCGCCGAGAACGGCAGCACATGGACGCGGAAGACCTACACCTACCCCAACGTGCAGGCGCCGGGCAACCTCTGGTACCACGACCACGCCCTCGGCCTCACCCGCGTCAGCCTCCTCGCCGGCCTCCTCGCCGCCTACGTCATCGAGAAGCCCGAGCTCGAGGACCCCATGAACCTCCCCTGCGGCGACCACGACCTCCACCTCGTCATCGCCGACCGCGAGTTCTACACCAATGGCTCCATCTCCATCGACCGGGAGTGGAAGCCGGAGTACTTCGGCCTCGTCATCACCGTCAACGGCAAGGCGTGGCCGTACCTCAGCGTCCAGCGCCGCCGCTACCGCCTCCGCATCCTCAACGCCAGCAACGCCAGATACTTCAACGTCACGCTCTCCAATGGCGCCCTCCCCTTCACCGTCATTGGCTCCGACTCCTCCTACCTCTCCAGGCCGGTCACCGTCTCCAACCTCGTCCTCTCGCCGGCCGAGATCTTCGACGTCATCGTCGACTTCTCCCGGTTGCCGGCGGCGATGACCGAGATCGAGATGCTCAACACGGCGCCCTACCCGTTCCCCAACGGCCCTAATGTGACGGACCCGAACCTCGACGGGAAGGTGATGCTGTTCAAGGTCGCCGGCAAGGGGAAGGTCGACGACATGCCGGACAAATCCAAGGTGCCGGAGCACGGCGTGCCGTACGCGAGCGTGGCGGCGCTCCCGCCGCCGACGACGACGAGGTACATCGTCCTGTACGAGAACCAGACCGCGCCGGGGAACCTCTACATCAACGGCCTCCGGCTGGAGGACCCGGTGACGGAGACGCCCAAGTCCGGCACGACGGAGCTGTGGCAGGTGATCAACCTCACCGGCGACAACCACCCGCTGCACCTCCACATTGCCACGTTCCAGGCCATCAAGATGACGAAGATCGAGGGATTCCAGGTGTTCAAGGACTGCATGATCAAAAACAACAACACCGCCACGTGCAACCTCGACCAGCACGCCGTCGGGCCCGTCGTGCCGGTGCCGGAGGAGGAGAAGACGTGGAAGAACGCGGTGAAGATTCCGCCGGAGTTCATGACGTCGGTGGTGGTGGCGTTCAGGCTTGTCGAGGCCAATCAGCCCTACCCCTTCGACGCCACCACCGAGCCGGGATTCGTCTACCACTGCCACATCCTGGATCACGAAGACAACGCCATGATCAGGCCTCTGAAGCTGCTTCCATGAACAAGAAACGTAGTGCTGATCGATGAGAATATGAGATGAAGAAGCTTCTTCACTAGGATTAATAATTCTGAGCATTTACGTTTGAGTGAAAGTTGTTTATGTTATGTTATCCAAGTCTTTGTTTATGAGTGAAAGTTTGCTAGTCATAAACTGGTGCGCTCTGACGGGGTATCTGTGCTACGGACTTAATTTGGGTTTGTACTTTATTGGAAGTTATATATGAATTATGTTGTTGATTGTTGAAGTACACTGTACAAAATAATATAGCTTATTTCAGGAAATAAATTTTAATCCTTCAAGGGA
CDS Sequence
- >LOC_Os01g03640.1
ATGAGTCCGAGGATTCAGCAGCTGGCCGCTGTGTTGTTGGCGGCTGTTGTCGTCGTCGCAGCCGCCAGGGATGAACCTGCAGCAGCAAAAAATTACCAAACTCAATGGGATACAGTAATGTCAATACTTAACTGTAAGAGTGATTCTTTAATCCCAAGCTACATCTGCAGCGTGATTAGCAAAAGTCGCTGGGGTTGGGCGTCAGATGACCCCAACGACGACGAGTACACACCACCGGATCATCCGCTGCCGGCGCCGGCGGCGGGGCGGCGGCGGTGGCCGGTGATGACGAGCTTGAACCTGACAAAGTACGTGGACTCGCTGCCGCGGATTGCCAAGATCCGGGGCTACGGCATCCGCCATGGCCGTCCAGTGCCCATCAAGCTCACCATCGGCATGTACAGCAAGACATGGCAATTCCACCGCGACATGCCACCGACGCCGGTGTTCGTGTACGGGCAATCGCTGCAGACAGCGACGTTCCCGGGGCCCACCATCGTGGCGCGGCAGGGCGTGCCGCTCGCCGTGGAGTGGCAGAACCACCTCCCCGACGCCCACATCCTCCCGTGGGACCCCAAGGTCCCCACCGCCATCCCCAAGAAGGGCGGCGTCCCCACCGTCGTTCACCTCCACGGCGGCGCCCACCCGCCGGAGTTCGACGGCCACGCCTTCGCCTGGTTCACCCGCGACTTCGCCGAGAACGGCAGCACATGGACGCGGAAGACCTACACCTACCCCAACGTGCAGGCGCCGGGCAACCTCTGGTACCACGACCACGCCCTCGGCCTCACCCGCGTCAGCCTCCTCGCCGGCCTCCTCGCCGCCTACGTCATCGAGAAGCCCGAGCTCGAGGACCCCATGAACCTCCCCTGCGGCGACCACGACCTCCACCTCGTCATCGCCGACCGCGAGTTCTACACCAATGGCTCCATCTCCATCGACCGGGAGTGGAAGCCGGAGTACTTCGGCCTCGTCATCACCGTCAACGGCAAGGCGTGGCCGTACCTCAGCGTCCAGCGCCGCCGCTACCGCCTCCGCATCCTCAACGCCAGCAACGCCAGATACTTCAACGTCACGCTCTCCAATGGCGCCCTCCCCTTCACCGTCATTGGCTCCGACTCCTCCTACCTCTCCAGGCCGGTCACCGTCTCCAACCTCGTCCTCTCGCCGGCCGAGATCTTCGACGTCATCGTCGACTTCTCCCGGTTGCCGGCGGCGATGACCGAGATCGAGATGCTCAACACGGCGCCCTACCCGTTCCCCAACGGCCCTAATGTGACGGACCCGAACCTCGACGGGAAGGTGATGCTGTTCAAGGTCGCCGGCAAGGGGAAGGTCGACGACATGCCGGACAAATCCAAGGTGCCGGAGCACGGCGTGCCGTACGCGAGCGTGGCGGCGCTCCCGCCGCCGACGACGACGAGGTACATCGTCCTGTACGAGAACCAGACCGCGCCGGGGAACCTCTACATCAACGGCCTCCGGCTGGAGGACCCGGTGACGGAGACGCCCAAGTCCGGCACGACGGAGCTGTGGCAGGTGATCAACCTCACCGGCGACAACCACCCGCTGCACCTCCACATTGCCACGTTCCAGGCCATCAAGATGACGAAGATCGAGGGATTCCAGGTGTTCAAGGACTGCATGATCAAAAACAACAACACCGCCACGTGCAACCTCGACCAGCACGCCGTCGGGCCCGTCGTGCCGGTGCCGGAGGAGGAGAAGACGTGGAAGAACGCGGTGAAGATTCCGCCGGAGTTCATGACGTCGGTGGTGGTGGCGTTCAGGCTTGTCGAGGCCAATCAGCCCTACCCCTTCGACGCCACCACCGAGCCGGGATTCGTCTACCACTGCCACATCCTGGATCACGAAGACAACGCCATGATCAGGCCTCTGAAGCTGCTTCCATGA
Protein Sequence
- >LOC_Os01g03640.1
MSPRIQQLAAVLLAAVVVVAAARDEPAAAKNYQTQWDTVMSILNCKSDSLIPSYICSVISKSRWGWASDDPNDDEYTPPDHPLPAPAAGRRRWPVMTSLNLTKYVDSLPRIAKIRGYGIRHGRPVPIKLTIGMYSKTWQFHRDMPPTPVFVYGQSLQTATFPGPTIVARQGVPLAVEWQNHLPDAHILPWDPKVPTAIPKKGGVPTVVHLHGGAHPPEFDGHAFAWFTRDFAENGSTWTRKTYTYPNVQAPGNLWYHDHALGLTRVSLLAGLLAAYVIEKPELEDPMNLPCGDHDLHLVIADREFYTNGSISIDREWKPEYFGLVITVNGKAWPYLSVQRRRYRLRILNASNARYFNVTLSNGALPFTVIGSDSSYLSRPVTVSNLVLSPAEIFDVIVDFSRLPAAMTEIEMLNTAPYPFPNGPNVTDPNLDGKVMLFKVAGKGKVDDMPDKSKVPEHGVPYASVAALPPPTTTRYIVLYENQTAPGNLYINGLRLEDPVTETPKSGTTELWQVINLTGDNHPLHLHIATFQAIKMTKIEGFQVFKDCMIKNNNTATCNLDQHAVGPVVPVPEEEKTWKNAVKIPPEFMTSVVVAFRLVEANQPYPFDATTEPGFVYHCHILDHEDNAMIRPLKLLP*