Gene Details:

Functional Descriptions:

  • qRT-PCR and promoter-GUS reporter analyses indicated that under Pi-sufficient conditions OsLPR5 was highly expressed in the roots, and specific expression occurred in the leaf collars and nodes, and its expression was increased under Pi-deficient conditions.
  • Overexpression of OsLPR5 triggered higher ferroxidase activity, and elevated concentrations of Fe(III) in the xylem sap and of total Fe in the roots and shoots.
  • Knockout mutation in OsLPR5 by means of CRISPR-Cas9 resulted in adverse effects on Pi translocation, on the relative expression of Cis-NATOsPHO1;2, and on several morphological traits, including root development and yield potential.
  • Our results indicate that ferroxidase-dependent OsLPR5 has both a broad-spectrum influence on growth and development in rice as well as affecting a subset of physiological and molecular traits that govern Pi homeostasis.
  • Transient expression of OsLPR5 in Nicotiana benthamiana provided evidence of its subcellular localization to the cell wall and endoplasmic reticulum.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os01g03640.1
    ATCGATCAGTCGATCAGCTAATCTCTTTCTCCTTTAATTCATTATCCTTTGCATGCCCCTCTCCAGCTAGCTAAGATGAGTCCGAGGATTCAGCAGCTGGCCGCTGTGTTGTTGGCGGCTGTTGTCGTCGTCGCAGCCGCCAGGGATGAACCTGCAGCAGCAAAAAATTACCAAACTCAATGGGATACAGTAATGTCAATACTTAACTGTAAGAGTGATTCTTTAATCCCAAGCTACATCTGCAGCGTGATTAGCAAAAGTCGCTGGGGTTGGGCGTCAGATGACCCCAACGACGACGAGTACACACCACCGGATCATCCGCTGCCGGCGCCGGCGGCGGGGCGGCGGCGGTGGCCGGTGATGACGAGCTTGAACCTGACAAAGTACGTGGACTCGCTGCCGCGGATTGCCAAGATCCGGGGCTACGGCATCCGCCATGGCCGTCCAGTGCCCATCAAGCTCACCATCGGCATGTACAGCAAGACATGGCAATTCCACCGCGACATGCCACCGACGCCGGTGTTCGTGTACGGGCAATCGCTGCAGACAGCGACGTTCCCGGGGCCCACCATCGTGGCGCGGCAGGGCGTGCCGCTCGCCGTGGAGTGGCAGAACCACCTCCCCGACGCCCACATCCTCCCGTGGGACCCCAAGGTCCCCACCGCCATCCCCAAGAAGGGCGGCGTCCCCACCGTCGTTCACCTCCACGGCGGCGCCCACCCGCCGGAGTTCGACGGCCACGCCTTCGCCTGGTTCACCCGCGACTTCGCCGAGAACGGCAGCACATGGACGCGGAAGACCTACACCTACCCCAACGTGCAGGCGCCGGGCAACCTCTGGTACCACGACCACGCCCTCGGCCTCACCCGCGTCAGCCTCCTCGCCGGCCTCCTCGCCGCCTACGTCATCGAGAAGCCCGAGCTCGAGGACCCCATGAACCTCCCCTGCGGCGACCACGACCTCCACCTCGTCATCGCCGACCGCGAGTTCTACACCAATGGCTCCATCTCCATCGACCGGGAGTGGAAGCCGGAGTACTTCGGCCTCGTCATCACCGTCAACGGCAAGGCGTGGCCGTACCTCAGCGTCCAGCGCCGCCGCTACCGCCTCCGCATCCTCAACGCCAGCAACGCCAGATACTTCAACGTCACGCTCTCCAATGGCGCCCTCCCCTTCACCGTCATTGGCTCCGACTCCTCCTACCTCTCCAGGCCGGTCACCGTCTCCAACCTCGTCCTCTCGCCGGCCGAGATCTTCGACGTCATCGTCGACTTCTCCCGGTTGCCGGCGGCGATGACCGAGATCGAGATGCTCAACACGGCGCCCTACCCGTTCCCCAACGGCCCTAATGTGACGGACCCGAACCTCGACGGGAAGGTGATGCTGTTCAAGGTCGCCGGCAAGGGGAAGGTCGACGACATGCCGGACAAATCCAAGGTGCCGGAGCACGGCGTGCCGTACGCGAGCGTGGCGGCGCTCCCGCCGCCGACGACGACGAGGTACATCGTCCTGTACGAGAACCAGACCGCGCCGGGGAACCTCTACATCAACGGCCTCCGGCTGGAGGACCCGGTGACGGAGACGCCCAAGTCCGGCACGACGGAGCTGTGGCAGGTGATCAACCTCACCGGCGACAACCACCCGCTGCACCTCCACATTGCCACGTTCCAGGCCATCAAGATGACGAAGATCGAGGGATTCCAGGTGTTCAAGGACTGCATGATCAAAAACAACAACACCGCCACGTGCAACCTCGACCAGCACGCCGTCGGGCCCGTCGTGCCGGTGCCGGAGGAGGAGAAGACGTGGAAGAACGCGGTGAAGATTCCGCCGGAGTTCATGACGTCGGTGGTGGTGGCGTTCAGGCTTGTCGAGGCCAATCAGCCCTACCCCTTCGACGCCACCACCGAGCCGGGATTCGTCTACCACTGCCACATCCTGGATCACGAAGACAACGCCATGATCAGGCCTCTGAAGCTGCTTCCATGAACAAGAAACGTAGTGCTGATCGATGAGAATATGAGATGAAGAAGCTTCTTCACTAGGATTAATAATTCTGAGCATTTACGTTTGAGTGAAAGTTGTTTATGTTATGTTATCCAAGTCTTTGTTTATGAGTGAAAGTTTGCTAGTCATAAACTGGTGCGCTCTGACGGGGTATCTGTGCTACGGACTTAATTTGGGTTTGTACTTTATTGGAAGTTATATATGAATTATGTTGTTGATTGTTGAAGTACACTGTACAAAATAATATAGCTTATTTCAGGAAATAAATTTTAATCCTTCAAGGGA
CDS Sequence
  • >LOC_Os01g03640.1
    ATGAGTCCGAGGATTCAGCAGCTGGCCGCTGTGTTGTTGGCGGCTGTTGTCGTCGTCGCAGCCGCCAGGGATGAACCTGCAGCAGCAAAAAATTACCAAACTCAATGGGATACAGTAATGTCAATACTTAACTGTAAGAGTGATTCTTTAATCCCAAGCTACATCTGCAGCGTGATTAGCAAAAGTCGCTGGGGTTGGGCGTCAGATGACCCCAACGACGACGAGTACACACCACCGGATCATCCGCTGCCGGCGCCGGCGGCGGGGCGGCGGCGGTGGCCGGTGATGACGAGCTTGAACCTGACAAAGTACGTGGACTCGCTGCCGCGGATTGCCAAGATCCGGGGCTACGGCATCCGCCATGGCCGTCCAGTGCCCATCAAGCTCACCATCGGCATGTACAGCAAGACATGGCAATTCCACCGCGACATGCCACCGACGCCGGTGTTCGTGTACGGGCAATCGCTGCAGACAGCGACGTTCCCGGGGCCCACCATCGTGGCGCGGCAGGGCGTGCCGCTCGCCGTGGAGTGGCAGAACCACCTCCCCGACGCCCACATCCTCCCGTGGGACCCCAAGGTCCCCACCGCCATCCCCAAGAAGGGCGGCGTCCCCACCGTCGTTCACCTCCACGGCGGCGCCCACCCGCCGGAGTTCGACGGCCACGCCTTCGCCTGGTTCACCCGCGACTTCGCCGAGAACGGCAGCACATGGACGCGGAAGACCTACACCTACCCCAACGTGCAGGCGCCGGGCAACCTCTGGTACCACGACCACGCCCTCGGCCTCACCCGCGTCAGCCTCCTCGCCGGCCTCCTCGCCGCCTACGTCATCGAGAAGCCCGAGCTCGAGGACCCCATGAACCTCCCCTGCGGCGACCACGACCTCCACCTCGTCATCGCCGACCGCGAGTTCTACACCAATGGCTCCATCTCCATCGACCGGGAGTGGAAGCCGGAGTACTTCGGCCTCGTCATCACCGTCAACGGCAAGGCGTGGCCGTACCTCAGCGTCCAGCGCCGCCGCTACCGCCTCCGCATCCTCAACGCCAGCAACGCCAGATACTTCAACGTCACGCTCTCCAATGGCGCCCTCCCCTTCACCGTCATTGGCTCCGACTCCTCCTACCTCTCCAGGCCGGTCACCGTCTCCAACCTCGTCCTCTCGCCGGCCGAGATCTTCGACGTCATCGTCGACTTCTCCCGGTTGCCGGCGGCGATGACCGAGATCGAGATGCTCAACACGGCGCCCTACCCGTTCCCCAACGGCCCTAATGTGACGGACCCGAACCTCGACGGGAAGGTGATGCTGTTCAAGGTCGCCGGCAAGGGGAAGGTCGACGACATGCCGGACAAATCCAAGGTGCCGGAGCACGGCGTGCCGTACGCGAGCGTGGCGGCGCTCCCGCCGCCGACGACGACGAGGTACATCGTCCTGTACGAGAACCAGACCGCGCCGGGGAACCTCTACATCAACGGCCTCCGGCTGGAGGACCCGGTGACGGAGACGCCCAAGTCCGGCACGACGGAGCTGTGGCAGGTGATCAACCTCACCGGCGACAACCACCCGCTGCACCTCCACATTGCCACGTTCCAGGCCATCAAGATGACGAAGATCGAGGGATTCCAGGTGTTCAAGGACTGCATGATCAAAAACAACAACACCGCCACGTGCAACCTCGACCAGCACGCCGTCGGGCCCGTCGTGCCGGTGCCGGAGGAGGAGAAGACGTGGAAGAACGCGGTGAAGATTCCGCCGGAGTTCATGACGTCGGTGGTGGTGGCGTTCAGGCTTGTCGAGGCCAATCAGCCCTACCCCTTCGACGCCACCACCGAGCCGGGATTCGTCTACCACTGCCACATCCTGGATCACGAAGACAACGCCATGATCAGGCCTCTGAAGCTGCTTCCATGA
Protein Sequence
  • >LOC_Os01g03640.1
    MSPRIQQLAAVLLAAVVVVAAARDEPAAAKNYQTQWDTVMSILNCKSDSLIPSYICSVISKSRWGWASDDPNDDEYTPPDHPLPAPAAGRRRWPVMTSLNLTKYVDSLPRIAKIRGYGIRHGRPVPIKLTIGMYSKTWQFHRDMPPTPVFVYGQSLQTATFPGPTIVARQGVPLAVEWQNHLPDAHILPWDPKVPTAIPKKGGVPTVVHLHGGAHPPEFDGHAFAWFTRDFAENGSTWTRKTYTYPNVQAPGNLWYHDHALGLTRVSLLAGLLAAYVIEKPELEDPMNLPCGDHDLHLVIADREFYTNGSISIDREWKPEYFGLVITVNGKAWPYLSVQRRRYRLRILNASNARYFNVTLSNGALPFTVIGSDSSYLSRPVTVSNLVLSPAEIFDVIVDFSRLPAAMTEIEMLNTAPYPFPNGPNVTDPNLDGKVMLFKVAGKGKVDDMPDKSKVPEHGVPYASVAALPPPTTTRYIVLYENQTAPGNLYINGLRLEDPVTETPKSGTTELWQVINLTGDNHPLHLHIATFQAIKMTKIEGFQVFKDCMIKNNNTATCNLDQHAVGPVVPVPEEEKTWKNAVKIPPEFMTSVVVAFRLVEANQPYPFDATTEPGFVYHCHILDHEDNAMIRPLKLLP*