Information report for WFSL1;STRIPE3
Gene Details
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Functional Descriptions
- The WFSL1 displayed white fine stripe leaves since tillering stage and abnormal chloroplast structure.
- RNA-seq analysis revealed that chloroplast development and photosynthesis genes were significantly affected in WFSL1 plants.
- Levels of WFSL1 and chloroplast encoded proteins were decreased in WFSL1 mutants via western blot analysis.
- Compared with WT, WFSL1 exhibits lower Chl content and defective in biogenesis of chloroplast ribosomes, which resulted in reduced grain yield.
- Taken together, our results show that WFSL1 is critical for chloroplast development, ribosome biogenesis, and light energy utilization, finally affects grain yield.
- Map-based cloning and Bioinformatic analysis indicated that WFSL1 on chromosome 1 contains an "A" to "T" substitution in protein coding region, and encodes a putative metal-dependent phosphohydrolase with HD domain at the N-terminus.
- Here, we isolated and characterized the STRIPE3 (st3) mutant which exhibited white-striped leaves with reduced chlorophyll content and abnormal chloroplast development during the seedling stage, but gradually produced nearly normal green leaves as it developed.
- In addition, the ROS accumulation level was significantly elevated, and the ROS scavenging enzyme activity was significantly decreased in WSL214 leaf tissue.
- As a result of elevated ROS levels, WSL214 leaf cells underwent DNA damage and programmed cell death.
- The WSL214 encodes an HD domain phosphohydrolase and is widely expressed in various tissues of rice, especially at the highest level in leaf tissue.
- Further research showed that WSL214 promoted the homeostasis of rice leaf cellular ROS in two ways.
- Compared to WT, WSL214 exhibited white-striped leaves, defective chloroplast development, reduced net photosynthetic rate, and overexcitation of photosynthetically active reaction centers.
- Second, WSL214 promoted chloroplast development, kept photosynthesis working properly, and reduced ROS produced by photosynthesis.
- WSL214 negatively regulates ROS accumulation and pathogen defense response in rice.
- However, WSL214 defense response to exogenous pathogens was also enhanced by high ROS levels.
- WSL214 plays an important role in promoting cellular ROS homeostasis by enhancing catalase activity and reducing photosynthetic ROS production.
- First, WSL214 increased the expression of the catalase gene OsCATC, making the intracellular ROS scavenging enzyme more active.
Functional Keywords
Literature and News
- Single-point Mutation of an Histidine-aspartic Domain-containing Gene involving in Chloroplast Ribosome Biogenesis Leads to White Fine Stripe Leaf in Rice . DOI: 10.1038/s41598-017-03327-2 ; PMID: 28607371
- STRIPE3, encoding a human dNTPase SAMHD1 homolog, regulates chloroplast development in rice . DOI: 10.1016/j.plantsci.2022.111395 ; PMID: 35878695
- WSL214 negatively regulates ROS accumulation and pathogen defense response in rice . DOI: 10.1007/s00299-022-02970-y ; PMID: 36585972
Sequences
cDNA Sequence
- >LOC_Os01g01920.1
AATCGCGCACAAGAAACGTAAACTCGTCCATACCTTAATTAATTATTATCCAAGCGCAAACATGAAACATCCCTCCCGCATTAAATTGGCATCTCCTAATAAATCTCACGGACTTCCATATCTACTCCCGGTTTCTGCTGGATTCCTTCCTTTTTCTCATCGCCTCGCCTGCCTCTTGGCCTTCCCCATCCCCATCTCTCATCAGCCGCAAACTGCTGCAAACCCCACTAGCGCTCGCCCCCACCGTAATCTCGCGCGATTCTCGGAGGAGGAGATGGGCGAGTACTGCGGCGCTGCTCCGGAGGAGGACCCTGCCATGGCCCTCGTCACGCCGCTCCCCACCACCACCACCACCACCACCACGGCGGCGGCGGCGGCCATCAAGCAACCCCACTACTACGGCTGCTTCGACCGCTGCTCCACCAAGCAGGTCTTCGACAACCTCCACGGCAACATCTCGCTCGACCCGTTGGCTCGTGAGTTTGTGGATACGGAGGAGTTTCAGAGGCTGCGGGATCTAAAACAACTTGGCCTTACATATCTGGTCTACCCAGGAGCCGTTCACACACGTTTTGAGCATTCATTAGGTGTCTATTGGCTAGCTGGGGAGGCTATGAACAATCTTCGACTGTACCAGGGAGAAGAGCTTGGCATTGACCGTGTTGATATGCAAACTGTAAAACTTGCAGGCCTTTTGCATGACATTGGACATGGCCCCTTCAGTCATTTGTTTGAACATGAGTTTCTTCCTCGTGTTGTTCCTGGATCAACCTGGACCCATGAAAATATGTCTGCACTGTTGCTGGACAGCATTGTCGACAAACACCAAATAGACATTGAAGCTGATCATCTCAAAATTGTTATGGAAATGATTGTTGCCAGCTCTAAGTTCACTGCAACAGAAAGCACAAAGGAGAAGCGCTTTCTTTATGACATTGTTGCTAATGGTCGGAATGGTATCGATGTTGACAAGTTTGACTACATTGGCCGTGATTGCAGAGCATGTGGTCTGGGTTGTAATTTCCAGTACTGGAGGCTCTTACAAGGCATGCGAGTCATGGGTGATGAAATATGCTACCCTGCCAAAGATTATTTGAGCATCCATAAATTGTTTACGACACGTGCTGATCTTCACCGCACTGTCTATACACATGCGAAAGTCAAGGCTGTTGAACTGATGCTTGTGGATGCACTTGTTGAGGCTAACGAATACTTGGGAATAGCTTTGCATGCACAAGATCCAGCGGACTTCTGGAAGTTAGATGACACGATCATTAAAAGCATTGAAACTGCTCCCAACGATGAACTGAACAAAGCAAAAGGGATCATTCAACGTATTCGCCGAAGAGAGCTCTATAAGTTCTGCAATGAATATTCTGTTCCAAAGGACAAGCTGGAGCACTTTAAGAATATAACCGCACAAGACATAGTTTGTTCACAGAAATCTTCCAAGGTGCTGCTGAAGGAAGAAGATGTTGCTGTTAGCAATGTTAAGATTGATTTGACCCGTGGAAAAGATAATCCCCTTGAAAGGTTTTTGATTTATAAACCCACTTTTCAGACTCTTATATTTCTTAGGGCGACATATCAGATTCTGACCTGTTACTTTTGTTGGTCTGCATATCTACTATCCCCAGCATCAAGTTCTTTAAGGATTTTGGTTGCGATGAGAAGTTCCCCATCACAGATGAGCGGGTCAGCCACCTGCTACCTGCGTACAACCAGGACAGAATTGTCCGGGTCTACGCCAAGAAGCACGAGCTGGTTGAAGCGGTGTCAGAAGCCTTCGAGAACCTACAACTGAGGATGTATGGTGAGAAGACACAAGTTCATGACACTCCCAGGAAGAAGCGCATTAGATTCCACTAATCTTCAGCAACTCCTTGTTGCCAAGTACTGTCATCAGTGTTAGTTAGTAGTAGCTCGTAGCATACTAGTACGACAAAGGAGAAAGGATCATAGGTAGACATACGGAAGGAAGGAAGGAACCTTTTATTACTGAGCGGGAGCATCCAATACTACTAATTACGGGTTTTGTTTTTGCTGGTAATCTGCACGCATAAGTAACGCGCAGATTAGTTGTACAGGTGATAGGCCACAGAAAATGTCTTGGGCAGTGGTGGTGTCACATGACGGCTGGCTACCATAGCAGATAGTAGTAGCTGATACGCCAGTATCGTGATCTTCTGATTTTTTTTTTACTCTGAGCCTGGGCTGTCTGGGACTGATGGCGTTGCTCCTGCTTTGCTAGTGAATGGATATATAATTATATTATATATATATATATATTGGCAAACAACGTTTGTTATGCTTACTATTCTCTC - >LOC_Os01g01920.2
AATCGCGCACAAGAAACGTAAACTCGTCCATACCTTAATTAATTATTATCCAAGCGCAAACATGAAACATCCCTCCCGCATTAAATTGGCATCTCCTAATAAATCTCACGGACTTCCATATCTACTCCCGGTTTCTGCTGGATTCCTTCCTTTTTCTCATCGCCTCGCCTGCCTCTTGGCCTTCCCCATCCCCATCTCTCATCAGCCGCAAACTGCTGCAAACCCCACTAGCGCTCGCCCCCACCGTAATCTCGCGCGATTCTCGGAGGAGGAGATGGGCGAGTACTGCGGCGCTGCTCCGGAGGAGGACCCTGCCATGGCCCTCGTCACGCCGCTCCCCACCACCACCACCACCACCACCACGGCGGCGGCGGCGGCCATCAAGCAACCCCACTACTACGGCTGCTTCGACCGCTGCTCCACCAAGCAGGTCTTCGACAACCTCCACGGCAACATCTCGCTCGACCCGTTGGCTCGTGAGTTTGTGGATACGGAGGAGTTTCAGAGGCTGCGGGATCTAAAACAACTTGGCCTTACATATCTGGTCTACCCAGGAGCCGTTCACACACGTTTTGAGCATTCATTAGGTGTCTATTGGCTAGCTGGGGAGGCTATGAACAATCTTCGACTGTACCAGGGAGAAGAGCTTGGCATTGACCGTGTTGATATGCAAACTGTAAAACTTGCAGGCCTTTTGCATGACATTGGACATGGCCCCTTCAGTCATTTGTTTGAACATGAGTTTCTTCCTCGTGTTGTTCCTGGATCAACCTGGACCCATGAAAATATGTCTGCACTGTTGCTGGACAGCATTGTCGACAAACACCAAATAGACATTGAAGCTGATCATCTCAAAATTGTTATGGAAATGATTGTTGCCAGCTCTAAGTTCACTGCAACAGAAAGCACAAAGGAGAAGCGCTTTCTTTATGACATTGTTGCTAATGGTCGGAATGGTATCGATGTTGACAAGTTTGACTACATTGGCCGTGATTGCAGAGCATGTGGTCTGGGTTGTAATTTCCAGTACTGGAGGCTCTTACAAGGCATGCGAGTCATGGGTGATGAAATATGCTACCCTGCCAAAGATTATTTGAGCATCCATAAATTGTTTACGACACGTGCTGATCTTCACCGCACTGTCTATACACATGCGAAAGTCAAGGCTGTTGAACTGATGCTTGTGGATGCACTTGTTGAGGCTAACGAATACTTGGGAATAGCTTTGCATGCACAAGATCCAGCGGACTTCTGGAAGTTAGATGACACGATCATTAAAAGCATTGAAACTGCTCCCAACGATGAACTGAACAAAGCAAAAGGGATCATTCAACGTATTCGCCGAAGAGAGCTCTATAAGTTCTGCAATGAATATTCTGTTCCAAAGGACAAGCTGGAGCACTTTAAGAATATAACCGCACAAGACATAGTTTGTTCACAGAAATCTTCCAAGGTGCTGCTGAAGGAAGAAGATGTTGCTGTTAGCAATGTTAAGATTGATTTGACCCGTGGAAAAGATAATCCCCTTGAAAGGTTTTTGATTTATAAACCCACTTTTCAGACTCTTATATTTCTTAGGGCGACATATCAGATTCTGACCTGTTACTTTTGTTGGTCTGCATATCTACTATCCCCAGCATCAAGTTCTTTAAGGATTTTGGTTGCGATGAGAAGTTCCCCATCACAGATGAGCGGGTCAGCCACCTGCTACCTGCGTACAACCAGGACAGAATTGTCCGGGTCTACGCCAAGAAGCACGAGCTGGTTGAAGCGGTGTCAGAAGCCTTCGAGAACCTACAACTGAGGATGTATGGTGAGAAGACACAAGTTCATGACACTCCCAGGAAGAAGCGCATTAGATTCCACTAATCTTCAGCAACTCCTTGTTGCCAAGTACTGTCATCAGTGTTAGTTAGTAGTAGCTCGTAGCATACTAGTACGACAAAGGAGAAAGGATCATAGGTAGACATACGGAAGGAAGGAAGGAACCTTTTATTACTGAGCGGGAGCATCCAATACTACTAATTACGGGTTTTGTTTTTGCTGGTAATCTGCACGCATAAGTAACGCGCAGATTAGTTGTACAGGTGATAGGCCACAGAAAATGTCTTGGGCAGTGGTGGTGTCACATGACGGCTGGCTACCATAGCAGATAGTAGTAGCTGATACGCCAGTATCGTGATCTTCTGATTTTTTTTTTACTCTGAGCCTGGGCTGTCTGGGACTGATGGCGTTGCTCCTGCTTTGCTAGTGAATGGATATATAATTATATTATATATATATATATATTGGCAAACAACGTTTGTTATGCTTACTATTCTCTC
CDS Sequence
- >LOC_Os01g01920.1
ATGAAACATCCCTCCCGCATTAAATTGGCATCTCCTAATAAATCTCACGGACTTCCATATCTACTCCCGGTTTCTGCTGGATTCCTTCCTTTTTCTCATCGCCTCGCCTGCCTCTTGGCCTTCCCCATCCCCATCTCTCATCAGCCGCAAACTGCTGCAAACCCCACTAGCGCTCGCCCCCACCGTAATCTCGCGCGATTCTCGGAGGAGGAGATGGGCGAGTACTGCGGCGCTGCTCCGGAGGAGGACCCTGCCATGGCCCTCGTCACGCCGCTCCCCACCACCACCACCACCACCACCACGGCGGCGGCGGCGGCCATCAAGCAACCCCACTACTACGGCTGCTTCGACCGCTGCTCCACCAAGCAGGTCTTCGACAACCTCCACGGCAACATCTCGCTCGACCCGTTGGCTCGTGAGTTTGTGGATACGGAGGAGTTTCAGAGGCTGCGGGATCTAAAACAACTTGGCCTTACATATCTGGTCTACCCAGGAGCCGTTCACACACGTTTTGAGCATTCATTAGGTGTCTATTGGCTAGCTGGGGAGGCTATGAACAATCTTCGACTGTACCAGGGAGAAGAGCTTGGCATTGACCGTGTTGATATGCAAACTGTAAAACTTGCAGGCCTTTTGCATGACATTGGACATGGCCCCTTCAGTCATTTGTTTGAACATGAGTTTCTTCCTCGTGTTGTTCCTGGATCAACCTGGACCCATGAAAATATGTCTGCACTGTTGCTGGACAGCATTGTCGACAAACACCAAATAGACATTGAAGCTGATCATCTCAAAATTGTTATGGAAATGATTGTTGCCAGCTCTAAGTTCACTGCAACAGAAAGCACAAAGGAGAAGCGCTTTCTTTATGACATTGTTGCTAATGGTCGGAATGGTATCGATGTTGACAAGTTTGACTACATTGGCCGTGATTGCAGAGCATGTGGTCTGGGTTGTAATTTCCAGTACTGGAGGCTCTTACAAGGCATGCGAGTCATGGGTGATGAAATATGCTACCCTGCCAAAGATTATTTGAGCATCCATAAATTGTTTACGACACGTGCTGATCTTCACCGCACTGTCTATACACATGCGAAAGTCAAGGCTGTTGAACTGATGCTTGTGGATGCACTTGTTGAGGCTAACGAATACTTGGGAATAGCTTTGCATGCACAAGATCCAGCGGACTTCTGGAAGTTAGATGACACGATCATTAAAAGCATTGAAACTGCTCCCAACGATGAACTGAACAAAGCAAAAGGGATCATTCAACGTATTCGCCGAAGAGAGCTCTATAAGTTCTGCAATGAATATTCTGTTCCAAAGGACAAGCTGGAGCACTTTAAGAATATAACCGCACAAGACATAGTTTGTTCACAGAAATCTTCCAAGGTGCTGCTGAAGGAAGAAGATGTTGCTGTTAGCAATGTTAAGATTGATTTGACCCGTGGAAAAGATAATCCCCTTGAAAGGTTTTTGATTTATAAACCCACTTTTCAGACTCTTATATTTCTTAGGGCGACATATCAGATTCTGACCTGTTACTTTTGTTGGTCTGCATATCTACTATCCCCAGCATCAAGTTCTTTAAGGATTTTGGTTGCGATGAGAAGTTCCCCATCACAGATGAGCGGGTCAGCCACCTGCTACCTGCGTACAACCAGGACAGAATTGTCCGGGTCTACGCCAAGAAGCACGAGCTGGTTGAAGCGGTGTCAGAAGCCTTCGAGAACCTACAACTGA - >LOC_Os01g01920.2
ATGAAACATCCCTCCCGCATTAAATTGGCATCTCCTAATAAATCTCACGGACTTCCATATCTACTCCCGGTTTCTGCTGGATTCCTTCCTTTTTCTCATCGCCTCGCCTGCCTCTTGGCCTTCCCCATCCCCATCTCTCATCAGCCGCAAACTGCTGCAAACCCCACTAGCGCTCGCCCCCACCGTAATCTCGCGCGATTCTCGGAGGAGGAGATGGGCGAGTACTGCGGCGCTGCTCCGGAGGAGGACCCTGCCATGGCCCTCGTCACGCCGCTCCCCACCACCACCACCACCACCACCACGGCGGCGGCGGCGGCCATCAAGCAACCCCACTACTACGGCTGCTTCGACCGCTGCTCCACCAAGCAGGTCTTCGACAACCTCCACGGCAACATCTCGCTCGACCCGTTGGCTCGTGAGTTTGTGGATACGGAGGAGTTTCAGAGGCTGCGGGATCTAAAACAACTTGGCCTTACATATCTGGTCTACCCAGGAGCCGTTCACACACGTTTTGAGCATTCATTAGGTGTCTATTGGCTAGCTGGGGAGGCTATGAACAATCTTCGACTGTACCAGGGAGAAGAGCTTGGCATTGACCGTGTTGATATGCAAACTGTAAAACTTGCAGGCCTTTTGCATGACATTGGACATGGCCCCTTCAGTCATTTGTTTGAACATGAGTTTCTTCCTCGTGTTGTTCCTGGATCAACCTGGACCCATGAAAATATGTCTGCACTGTTGCTGGACAGCATTGTCGACAAACACCAAATAGACATTGAAGCTGATCATCTCAAAATTGTTATGGAAATGATTGTTGCCAGCTCTAAGTTCACTGCAACAGAAAGCACAAAGGAGAAGCGCTTTCTTTATGACATTGTTGCTAATGGTCGGAATGGTATCGATGTTGACAAGTTTGACTACATTGGCCGTGATTGCAGAGCATGTGGTCTGGGTTGTAATTTCCAGTACTGGAGGCTCTTACAAGGCATGCGAGTCATGGGTGATGAAATATGCTACCCTGCCAAAGATTATTTGAGCATCCATAAATTGTTTACGACACGTGCTGATCTTCACCGCACTGTCTATACACATGCGAAAGTCAAGGCTGTTGAACTGATGCTTGTGGATGCACTTGTTGAGGCTAACGAATACTTGGGAATAGCTTTGCATGCACAAGATCCAGCGGACTTCTGGAAGTTAGATGACACGATCATTAAAAGCATTGAAACTGCTCCCAACGATGAACTGAACAAAGCAAAAGGGATCATTCAACGTATTCGCCGAAGAGAGCTCTATAAGTTCTGCAATGAATATTCTGTTCCAAAGGACAAGCTGGAGCACTTTAAGAATATAACCGCACAAGACATAGTTTGTTCACAGAAATCTTCCAAGGTGCTGCTGAAGGAAGAAGATGTTGCTGTTAGCAATGTTAAGATTGATTTGACCCGTGGAAAAGATAATCCCCTTGAAAGGTTTTTGATTTATAAACCCACTTTTCAGACTCTTATATTTCTTAGGGCGACATATCAGATTCTGACCTGTTACTTTTGTTGGTCTGCATATCTACTATCCCCAGCATCAAGTTCTTTAAGGATTTTGGTTGCGATGAGAAGTTCCCCATCACAGATGAGCGGGTCAGCCACCTGCTACCTGCGTACAACCAGGACAGAATTGTCCGGGTCTACGCCAAGAAGCACGAGCTGGTTGAAGCGGTGTCAGAAGCCTTCGAGAACCTACAACTGA
Protein Sequence
- >LOC_Os01g01920.1
MKHPSRIKLASPNKSHGLPYLLPVSAGFLPFSHRLACLLAFPIPISHQPQTAANPTSARPHRNLARFSEEEMGEYCGAAPEEDPAMALVTPLPTTTTTTTTAAAAAIKQPHYYGCFDRCSTKQVFDNLHGNISLDPLAREFVDTEEFQRLRDLKQLGLTYLVYPGAVHTRFEHSLGVYWLAGEAMNNLRLYQGEELGIDRVDMQTVKLAGLLHDIGHGPFSHLFEHEFLPRVVPGSTWTHENMSALLLDSIVDKHQIDIEADHLKIVMEMIVASSKFTATESTKEKRFLYDIVANGRNGIDVDKFDYIGRDCRACGLGCNFQYWRLLQGMRVMGDEICYPAKDYLSIHKLFTTRADLHRTVYTHAKVKAVELMLVDALVEANEYLGIALHAQDPADFWKLDDTIIKSIETAPNDELNKAKGIIQRIRRRELYKFCNEYSVPKDKLEHFKNITAQDIVCSQKSSKVLLKEEDVAVSNVKIDLTRGKDNPLERFLIYKPTFQTLIFLRATYQILTCYFCWSAYLLSPASSSLRILVAMRSSPSQMSGSATCYLRTTRTELSGSTPRSTSWLKRCQKPSRTYN* - >LOC_Os01g01920.2
MKHPSRIKLASPNKSHGLPYLLPVSAGFLPFSHRLACLLAFPIPISHQPQTAANPTSARPHRNLARFSEEEMGEYCGAAPEEDPAMALVTPLPTTTTTTTTAAAAAIKQPHYYGCFDRCSTKQVFDNLHGNISLDPLAREFVDTEEFQRLRDLKQLGLTYLVYPGAVHTRFEHSLGVYWLAGEAMNNLRLYQGEELGIDRVDMQTVKLAGLLHDIGHGPFSHLFEHEFLPRVVPGSTWTHENMSALLLDSIVDKHQIDIEADHLKIVMEMIVASSKFTATESTKEKRFLYDIVANGRNGIDVDKFDYIGRDCRACGLGCNFQYWRLLQGMRVMGDEICYPAKDYLSIHKLFTTRADLHRTVYTHAKVKAVELMLVDALVEANEYLGIALHAQDPADFWKLDDTIIKSIETAPNDELNKAKGIIQRIRRRELYKFCNEYSVPKDKLEHFKNITAQDIVCSQKSSKVLLKEEDVAVSNVKIDLTRGKDNPLERFLIYKPTFQTLIFLRATYQILTCYFCWSAYLLSPASSSLRILVAMRSSPSQMSGSATCYLRTTRTELSGSTPRSTSWLKRCQKPSRTYN*