Gene Details:

• MSU gene ID: LOC_Os01g01920
• RAPdb gene ID: Os01g0109300
• Gene Symbol: WFSL1 STRIPE3 WSL214
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• The WFSL1 displayed white fine stripe leaves since tillering stage and abnormal chloroplast structure.
• RNA-seq analysis revealed that chloroplast development and photosynthesis genes were significantly affected in WFSL1 plants.
• Levels of WFSL1 and chloroplast encoded proteins were decreased in WFSL1 mutants via western blot analysis.
• Compared with WT, WFSL1 exhibits lower Chl content and defective in biogenesis of chloroplast ribosomes, which resulted in reduced grain yield.
• Taken together, our results show that WFSL1 is critical for chloroplast development, ribosome biogenesis, and light energy utilization, finally affects grain yield.
• Map-based cloning and Bioinformatic analysis indicated that WFSL1 on chromosome 1 contains an "A" to "T" substitution in protein coding region, and encodes a putative metal-dependent phosphohydrolase with HD domain at the N-terminus.
• Here, we isolated and characterized the STRIPE3 (st3) mutant which exhibited white-striped leaves with reduced chlorophyll content and abnormal chloroplast development during the seedling stage, but gradually produced nearly normal green leaves as it developed.
• In addition, the ROS accumulation level was significantly elevated, and the ROS scavenging enzyme activity was significantly decreased in WSL214 leaf tissue.
• As a result of elevated ROS levels, WSL214 leaf cells underwent DNA damage and programmed cell death.
• The WSL214 encodes an HD domain phosphohydrolase and is widely expressed in various tissues of rice, especially at the highest level in leaf tissue.
• Further research showed that WSL214 promoted the homeostasis of rice leaf cellular ROS in two ways.
• Compared to WT, WSL214 exhibited white-striped leaves, defective chloroplast development, reduced net photosynthetic rate, and overexcitation of photosynthetically active reaction centers.
•  Second, WSL214 promoted chloroplast development, kept photosynthesis working properly, and reduced ROS produced by photosynthesis.
• WSL214 negatively regulates ROS accumulation and pathogen defense response in rice.
• However, WSL214 defense response to exogenous pathogens was also enhanced by high ROS levels.
• WSL214 plays an important role in promoting cellular ROS homeostasis by enhancing catalase activity and reducing photosynthetic ROS production.
• First, WSL214 increased the expression of the catalase gene OsCATC, making the intracellular ROS scavenging enzyme more active.

Function-related keywords:

• chloroplast ,  tillering ,  grain ,  development ,  map-based-cloning ,  photosynthesis ,  grain-yield ,  yield ,  chloroplast-development ,  seedling ,  chlorophyll ,  chlorophyll-content ,  leaf ,  defense-response ,  defense ,  cell-death ,  pathogen ,  homeostasis ,  programmed-cell-death ,  catalase

Literature:

• Single-point Mutation of an Histidine-aspartic Domain-containing Gene involving in Chloroplast Ribosome Biogenesis Leads to White Fine Stripe Leaf in Rice .  DOI:  10.1038/s41598-017-03327-2 ;  PMID:  28607371
• STRIPE3, encoding a human dNTPase SAMHD1 homolog, regulates chloroplast development in rice .  DOI:  10.1016/j.plantsci.2022.111395 ;  PMID:  35878695
• WSL214 negatively regulates ROS accumulation and pathogen defense response in rice .  DOI:  10.1007/s00299-022-02970-y ;  PMID:  36585972

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: