In vivo and in vitro experiments showed that MtLUX directly binds to a cis-element in the promoter of MtTB1/MtTCP1A, suggesting that MtLUX regulates branching by rhythmically suppressing MtTB1/MtTCP1A.
Core clock component MtLUX controls shoot architecture through repression of MtTB1/MtTCP1A in Medicago truncatula.
Gene expression analysis revealed that MtLUX regulated shoot architecture by repressing the expression of strigolactone receptor MtD14 and MtTB1/MtTCP1A, a TCP gene that functions centrally in modulating shoot architecture.
Our results provide direct evidence that the core clock component MtLUX directly regulates plant architecture.