Gene Details:

Functional Descriptions:

  • Phloretin glycosyltransferase2 (PGT2) was identified by activity-directed protein purification and differential gene expression analysis in samples high in trilobatin but low in phloridzin.
  • Transient expression of double bond reductase, chalcone synthase, and PGT2 genes reconstituted the apple pathway for trilobatin production in Nicotiana benthamiana.
  • Transgenic M. × domestica plants overexpressing PGT2 produced high concentrations of trilobatin in young leaves.
  • Biochemical analysis showed PGT2 efficiently catalyzed 4′-O-glycosylation of phloretin to trilobatin as well as 3-hydroxyphloretin to sieboldin.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
CDS Sequence
  • >MDP0000836043
    ATGGAGGCGACAGCTATAGTTTTATATCCATCACCTCTAATTGGGCACTTAGTCTCCATGGTAGAGCTAGGCAAGCTCATACTCACCCGCCACCCTTCTCTGTGCATCCACATCCTCATCACCACCCCGCCCTACCGTGCCAACGACACCGACTCATACATCACCTCCGTCTCCGCCGCCAACCCTTCCCTCATTTTCCACCACCTCCCCACCATCTCCCTCCCTCCCTCCCTCTCCCCCTCCCGCAACCACGAAACCCCAATCTTCGAAGTCCTTCTCCTCAACAACCCTTACGTCCACCAAGCCCTCCTCTCCATCTCCCACAACTTCTCCATCAAAGCTTTTGTCATGGACTTCTTCTGCTCTGTCGGGCTCCCCATTGCCACCGAGCTGAACATCCCCAGCTACTTCTTCTTCACATCCAGCGCCGCCAACCTCGCTTGCTTCCTCTACCTCCCCACCATTCACAGCATCACTGACAAAAGCCTCAAAGACCTAAATATCCTTCTCAACATTCCAGGAGTCCAGCCGATTCCTTCCTCCGATATGCCGAAACCGATTCTTGAACGAAACAACAAAGTGTATGAACATTTCCAAGAAAGCTCAAAGCAGTTCCCGAAATCAGCTGGGATTATCGTAAACACGTTTGAATCTCTCGAACCCAGAGTTCTCAGAGCAATATGGGACGGTCTGTGCTTGACGGAGAACGTTCCAACTCCACCGGTCTACCCCATCGGACCGCTGATTATTTCCCATGGCGGTGGAGGCCGCGGGGCCGAGTATTTGAAATGGCTGGACTCACAGCCAAGTGGAAGCGTGGTGTTCCTCTGTTTTGGGAGCTTGGGATTGTTTTCAAAGGAGCAGTTGAAGGAAATTGCGATTGGGTTGGAGAATAGTGGGCACAGATTTTTGTGGGTGGTCCGTAATCCTCCAGCCCAAAATCAAATTGGGCTGGCTATTAAAGAGTCCGATCCGGAATTGAAATCTTTGCTTCCGGACGGGTTCTTGGATCGGACTAAGGGTCGGGGTCTCGTGGTCAAGTCATGGGCCCCGCAAGTGGCAGTGTTGAATCACAACTCGGTGGGTGGGTTTGTGAGTCATTGCGGGTGGAACTCGGTGTTGGAATCGGTGTGTGCCGGTGTGCCGATTGTGGCTTGGCCGCTCTACGCGGAGCAGAGATTCAATCGAGTGGTTTTGGTGGAGGAGATTAAGATTGCTATGCCGATGAACGAGTCAGAAGACGGGTTTGTGAGAGCAGCGGAGGTGGAGAAGCGAGTTACGGAGTTGATGGACTCGGAGGAGGGCGAGTCGATCAGGAAGCGTACAAAGGATTTGCAAAACGATGCCCATGCAGCATTGGGTGAGACCGGGTCGTCTCGGGTTGCATTTACTAAACTACTTGAATTCTGGGGCAAAACCTGTTAG
Protein Sequence
  • >MDP0000836043
    MEATAIVLYPSPLIGHLVSMVELGKLILTRHPSLCIHILITTPPYRANDTDSYITSVSAANPSLIFHHLPTISLPPSLSPSRNHETPIFEVLLLNNPYVHQALLSISHNFSIKAFVMDFFCSVGLPIATELNIPSYFFFTSSAANLACFLYLPTIHSITDKSLKDLNILLNIPGVQPIPSSDMPKPILERNNKVYEHFQESSKQFPKSAGIIVNTFESLEPRVLRAIWDGLCLTENVPTPPVYPIGPLIISHGGGGRGAEYLKWLDSQPSGSVVFLCFGSLGLFSKEQLKEIAIGLENSGHRFLWVVRNPPAQNQIGLAIKESDPELKSLLPDGFLDRTKGRGLVVKSWAPQVAVLNHNSVGGFVSHCGWNSVLESVCAGVPIVAWPLYAEQRFNRVVLVEEIKIAMPMNESEDGFVRAAEVEKRVTELMDSEEGESIRKRTKDLQNDAHAALGETGSSRVAFTKLLEFWGKTC*