Gene Details:

  • Gene ID: MD06G1128500
  • Gene Symbol: MxRBOHD2
  • Gene Name: Malus xiaojinensis Mitogen-activated protein kinase 4-1
  • Genome: Golden ASM211411v1
  • Species: Malus domestica

Functional Descriptions:

  • MxMPK4-1 phosphorylated the C terminus of MxRBOHD2 at Ser797 and Ser906 and positively and negatively regulated NOX activity through these phospho-sites, respectively. When compared with apple calli that overexpressed MxRBOHD2, the coexpression of MxMPK4-1 and MxRBOHD2 prominently enhanced the Fe deficiency responses.
  • We found that MxMPK4-1 interacted with NADPH oxidases (NOX)—respiratory burst oxidase homologs MxRBOHD1 and MxRBOHD2, which positively regulated responses to Fe deficiency.
  • When compared with apple calli that overexpressed MxRBOHD2, the coexpression of MxMPK4-1 and MxRBOHD2 prominently enhanced the Fe deficiency responses.
  • MxMPK4-1 phosphorylated the C terminus of MxRBOHD2 at Ser797 and Ser906 and positively and negatively regulated NOX activity through these phospho-sites, respectively.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
mRNA Sequence
  • />MD06G1128500
    GACTGATTCTAACAAAACTCGTAGATAGAATATACTTTCTTTGAAGTGGGAATGATCTGACCTGCTCTATAAATAATATAAACATCACATGCATACACTCCATCCACTAAGCCCCAGAAAGAAATGAAAGAAACAGAAAATAATTAACAACCCTTTTGGACTCTTCTCTCATTGCCAGCCTTTCCTTTGTTATAAGCAAAGAAACACACCAAGATCTTCAGCCTTAAAATTTCTTCCTCACAGAAACTCGTTTGGTTACTGAGAAAATGGGAACAAATGAGGCTGGAGGAGTTCATTACCACTCAGATACTGAGATCATCGGTGGTAACGAAAAAATGGCGAATTATAGCGGTCCGCTGAGCGGACCTCTTAGCGGACCACTGAACAAAAAAAGATCAGGCACAAAGAAGAATGCCAAGATCAACACACCGGAGCCAATCTCTGCTACCAAGCCTGGCATTAGTTCAAATGATGAAGAATCTTATGTCGAGGTCACTCTGGACGTCCGAGATGATTCTGTGGCGGTTCACAGCGTAAAACCTGCCGATCATCCTCCTCATGAAGATCACGAAGATCATGTTGAGCTGCTGGGAAAAGGGTTGGAGAAGAGCCGGTCTTTTGTTCGAAATGCTTCATTTCATATCAAGCGCCTGGCCTCGTTTTCAAAACAAGCAGTAGTAGCAGCAGTGCCTCATCATCATCCGCGAAGGCGGTTTGATCGAACACAGTCAGCGGCTGCCCCTGCATTGAAGGGCCTCAAGTTCATCAGCAAGACTGATGGGAATGAGTGGCCTGAAGTTGAGAAGAGGTTCTGCAAGCTCACTGCTTCCACCAATGGCCTGCTTTCCCGTGCGCTTTTCGGGGAATGTATAGGAATGAACAAGGAATCGAAAGAGTTTGCAGGGAAGCTTTTCGATGCGCTTGCTCGAAAGCTCGACATCAAAGGCGATTCTATCAATGAGGATCAGCTGAAAGAGTTATGGGATCAAATCTCTGAGCAGGGATTTGACTCCAGGCTTCAAACTTTCTTTGACATGGTTGATAAAGATGCAGATGGAAGAATCACCATAGAGGAAGTCAAGGAGATTATTAGCTTCAGTGCTTCTGCCAACAAGCTCTCAAACATCCAAAAACAAGCAGATGAGTATGCAGCATTGATCATGGAAGAGTTAGATCCAGACAATCTCGGATACATCATGATAGACAGCCTCGAAACGCTCTTGTTGGAAGGACCGGAAGAGACTGCAGCTGCGGCAAGAGGAAAAGACAGCCGAAACCTAAGCAGAATGCTGAGCCAGAAGCTCAAGCCTACAAGAGAGCATCCATTAACAAGGTGTTATAACAATACAAAGTACTTCTTGCAAGACAATTGGAAGAGAGTTTGGGTAATGATCCTGTGGCTCGGAATCACGTTAGGTCTATTCGTGTACAAATTCATGCAGTATCGAAACAAGGCAGCGTACGAAGTGATGGGACACTGCGTCTGCATGGCGAAAGGTGCAGCGGAGACACTCAAATTCAACATGGCGCTCATTCTCCTCCCGGTCTGCCGGAACACCATAACCTTGCTCCGAAACAAGACCAAACTAGGAGGCGTTATCCCCTTCGACGACAATCTCAATTTCCACAAGGTGATAGCTGTGGGGATTGGGTTTGGAGTTGGGATACATGCAATCTACCATATGGCCTGCGACTTCCCGAGGCTCATTCATGCGAACAGCGATGACTACGAGCTGATGAAGCCATTTTTCGGGGAGGTGCAACCTTCGAGTTACTGGTATTTCGTGAAATCTTTGGAGGGAGTGAGTGGGATTGTCATGGTGGTTTTGATGGCGGTTGCATTCACATTGGCTGCTCCTTGTTTCAGAAGAGGTAAAGTTAACCTGCCTAAGCCTCTGAATAAGCTCGCTGGGTTCAATGCCTTTTGGTATTCCCACCACCTTTTCGTCATAGTCTATGTCCTGCTGATTATCCATGGAGTTCACCTGTATCTGACCAAGAAATGGTACCAGAAAACAACATGGATGTACCTAGCTGTTCCACTCACACTCTATGCTGGTGAAAGATTAGTTAGGGCACTCAGATCCAGCATCAAGCCTGTTCAGATTCTAAAGGTGGCTCTCTACCCTGGTAATGTCTTGGCACTTCATTTGTCCAAGCCTCAAGGCTTCAGATACCATAGTGGGCAATACATGTTTGTTAATTGTGCTGCTGTGTCGCCATTTGAATGGCACCCGTTTTCCATCACCTCGGCCCCTGGAGATGACCACCTCAGTGTTCATATCCGGATCGTCGGAGATTGGACCCGACAACTTAAAACGGTGTTCTCAAAGGTATGCCAACCACCTCCTAGTGGGAAAAGTGGACTCCTTACAGCTGACCACATGCAAGGAGCTGAAAACTTGATATTTCCAAGAGTGTTGATCGATGGACCCTATGGAGCACCAGCTCAAGAATACAAGACCTATGAAGTGGTGTTGTTGGTAGGGCTAGGGATTGGGGCAACCCCAATGATTAGCATTTTGAAGGACATAGTGAACAATATCAAGGCCATGGAGGAAGAGGAGGAGGACAATGCTTTAGAAAATGGTGGTGGTGGTGGTGGCGGCACCAAGTTACCACACAAATCATCAAAAACACATGCTGGGTCGACCTCGGTGGGACACCATAACTTTAAGACCAAGCGGGCGTATTTTTATTGGGTGACACGGGAACAGGGCTCCTTTGATTGGTTCAAAGGAGCGATGAATGAGGTGGCGGAATTGGACCACAACAGGGTCATTGAATTTCACAATTATTGCACTAGTGTGTATGAAGAAGGTGATGCACGTTCTGCCCTCATTGCCATGCTTCAGTCTTTGAGTCATGCAAAGAATGGTGTCGACATTGTCTCCGGCACACGTGTCAAATCGCACTTTGCCAAGCCAAACTGGCGTGAGGTCTACAAGCGGATTGCACTCGATCACACCGATGCTAGAGTTGGGGTGTTCTACTGCGGGCCACCGGCGCTTACCGAAGAGCTACGCCAGCTGTCCCTCGACTTCTCTCACAAAACCTCCACCCGATTTGAGTTCCACAAAGAAAACTTCTGATAACACAAGTCTGTCCCACACATTATATATATAATTATAATTATACAAGTTAATTACAGGTTGGATTGAAGAGATGTGCAAACCATGTTATACAATGATGCAATCGTTGTAATCTCTTATTATCATGTGAGATGAGAGAGAATAAGAGAAGATAATATTTTGTAATGATAGAATCTCTCAGGTTTTAGGAGAAATTCTTGGGTACGGCTGACGCAGCAACACACTCATTAGGATTTGAGACTCACATGTAAAGTCGCATATTGTTAAGGTATTGTTGTGTGCAGCAGTCGCATCTTAAATTCTCCCATATTGTAGCATAAAGTTTGTATTCGAGTTATTTTCTTTTTCCTTAACTTATACATAGAAGAGAATGTGGATGTATTTTTAAGTCATATATGATAATCATATAAGGTTGTGTTTGGTCAAGAAGATAATTTCCCAAGGGAACTTCCCAGTGTAATGGCAAGCTTGTAATTTCACAAGCTTCTGGTACTTATACGACTCACAAGTTTAAACGGC
Protein Sequence
  • />MD06G1128500
    MGTNEAGGVHYHSDTEIIGGNEKMANYSGPLSGPLSGPLNKKRSGTKKNAKINTPEPISATKPGISSNDEESYVEVTLDVRDDSVAVHSVKPADHPPHEDHEDHVELLGKGLEKSRSFVRNASFHIKRLASFSKQAVVAAVPHHHPRRRFDRTQSAAAPALKGLKFISKTDGNEWPEVEKRFCKLTASTNGLLSRALFGECIGMNKESKEFAGKLFDALARKLDIKGDSINEDQLKELWDQISEQGFDSRLQTFFDMVDKDADGRITIEEVKEIISFSASANKLSNIQKQADEYAALIMEELDPDNLGYIMIDSLETLLLEGPEETAAAARGKDSRNLSRMLSQKLKPTREHPLTRCYNNTKYFLQDNWKRVWVMILWLGITLGLFVYKFMQYRNKAAYEVMGHCVCMAKGAAETLKFNMALILLPVCRNTITLLRNKTKLGGVIPFDDNLNFHKVIAVGIGFGVGIHAIYHMACDFPRLIHANSDDYELMKPFFGEVQPSSYWYFVKSLEGVSGIVMVVLMAVAFTLAAPCFRRGKVNLPKPLNKLAGFNAFWYSHHLFVIVYVLLIIHGVHLYLTKKWYQKTTWMYLAVPLTLYAGERLVRALRSSIKPVQILKVALYPGNVLALHLSKPQGFRYHSGQYMFVNCAAVSPFEWHPFSITSAPGDDHLSVHIRIVGDWTRQLKTVFSKVCQPPPSGKSGLLTADHMQGAENLIFPRVLIDGPYGAPAQEYKTYEVVLLVGLGIGATPMISILKDIVNNIKAMEEEEEDNALENGGGGGGGTKLPHKSSKTHAGSTSVGHHNFKTKRAYFYWVTREQGSFDWFKGAMNEVAELDHNRVIEFHNYCTSVYEEGDARSALIAMLQSLSHAKNGVDIVSGTRVKSHFAKPNWREVYKRIALDHTDARVGVFYCGPPALTEELRQLSLDFSHKTSTRFEFHKENF