Gene Details:

Functional Descriptions:

  • Transcriptome deep-sequencing analysis identified the core transcription factor gene MdWRKY53, whose expression responded to both KCl and MT treatment.
  • Overexpressing MdWRKY53 enhanced KCl tolerance in transgenic apple plants by increasing K+ efflux and K+ compartmentalization.
  • RT–qPCR and LUC activity analyses indicated that MdWRKY53 binds to the promoters of MdGORK1 and MdNHX2 and induces their transcription.
  • To explore the role of MdWRKY53 in apple under KCl stress and MT treatment, we transformed apple with this gene and analysed its regulatory mechanism by chromatin immunoprecipitation followed by sequencing (ChIP-seq).

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
mRNA Sequence
  • />MD06G1104100
    CTCTCAAATTTGTCCGTTCAAATCAAAACTCTCTCACTCTCTTTGCTTCTGCAGCTTCACTACTCTAATCTTCTTCCCTTTTCCTGCATACTCAATTCCTCACTTCAAATAATCTTCAAATTCTGGATACCAATTCCATGTCTGTACCTTCTTACTGCAAACAACGTTTCGGAGCTCACGAATCTCCTTGGGTTCTACTCTTTCTCGGAATTGCGAAGCTTTAGTGGGATGAAGTTGATGAAGGTGCTTAAACACTGCTCATAATCTTCCACGGTACTTGTAGTTCACGTGGTTAAGAGTGTCTAAGGTCCTGTGTTCAAATCCTCCCTTCCCCGATATCGCTTGTATCAGGAAGAAAAAATCATTGCTCATAATCCCAACTGACATATAGAATTGAAGAAATGGATTCTGGTAAGAGCTGGGAGCAGAAGTCACTGGTCAATGAGATCATTGAAGGAATGGAGCTAGCAAAACAGTTGAGGCTATGTCTAAATGCAAAATCGTCAGCAGACACCAAGCAATTTTTAGTGCAGAGGATACTGTCGTCATATGAGAAGGCCCTTCTGATGCTGAAATGTGGCGGTTCGCCACAAAGTCCTATCAGAGCAATTGCTAGCGCGCCGGAGTCGCTAATGTCAGCCAATGAAGGTCCTTGTTGCGTTGACAATAACAGAAGTCTCCAGGATCATCAGGACCTGACGGCGGTCTCCAAGAAAAGAAAGGAAATGGCCAAATGGACAGAACACGTCACGAGAGTAATCTCTGAGAATGGGATTGAAGGACCCCATGAAGATGGCCACAGCTGGAGAAAATATGGGCAGAAAGACATCCTAGGAGCCAAACATCCAAGAAGCTATTACAGATGCACGTACCGGAACACGCAAAGTTGTTGGGCTACAAAGCAAGTGCAAAGATCAGATGAAGACCCCACCGTCTTCGAAATCACATACAAAGGGAAGCATACATGTTCTCATGGCGGCAGTTCAGTTCCACCGCCACCATCACCAGAAAAGCAAGAACGAAAACGACACAATCATAACAATACTTATCAACAACAGCAGTCTCAAGGAAACCAAATGAGCTTCCCAACTAATCTGAGAGTCAATACTGAGAACTTAGACGACAGAGAGAACACAGCATCTCCATTCTCTTTTACTTCATCTCCATTCGGAAGTATCAGCGATGACGCCTTCCTATCTTCGATGCTTGATGATCAAAGTCTTTTTGACCATTTCAATCAATCATTGCTGTCTCCAGCCGCAGGCGGATCAAACTATTACTTTGAGCTGCCCAGCCAGATGAGAAACATTGCAGGAAATGAGCAACGTTCGGAATCTGATATCATCTCAGCCAACAATTCGTCAACCAATTCTCCGATCCCGGACATGGATTTTCCACTGGAGCCAGTGGAACTCGACCCCTATTTCCCATTTGACTCCCCAGGATTTTTCTTATAATTCTAGTCGGTCTTTGAGAATAAGAATAAGTT
Protein Sequence
  • />MD06G1104100
    MDSGKSWEQKSLVNEIIEGMELAKQLRLCLNAKSSADTKQFLVQRILSSYEKALLMLKCGGSPQSPIRAIASAPESLMSANEGPCCVDNNRSLQDHQDLTAVSKKRKEMAKWTEHVTRVISENGIEGPHEDGHSWRKYGQKDILGAKHPRSYYRCTYRNTQSCWATKQVQRSDEDPTVFEITYKGKHTCSHGGSSVPPPPSPEKQERKRHNHNNTYQQQQSQGNQMSFPTNLRVNTENLDDRENTASPFSFTSSPFGSISDDAFLSSMLDDQSLFDHFNQSLLSPAAGGSNYYFELPSQMRNIAGNEQRSESDIISANNSSTNSPIPDMDFPLEPVELDPYFPFDSPGFFL