Gene Details:

Functional Descriptions:

  • The first committed carotenoid pathway step in apple is controlled by MdPSY1 and MdPSY2, while MdPSY4 play little or no role in this respect.
  • MdPSY1 and MdPSY2 constructs in cells with pACCRTE produced phytoene, with a peak whose retention time and spectral qualities were similar to the positive control. Similarly, ΔcrtB cells produced zeaxanthin diglucoside when transformed with MdPSY1 and MdPSY2 constructs.
  • Both MdPSY1 and MdPSY2 were co-localised with chlorophyll in the chloroplast confirming they are translocated to plastids.
  • The possibility of different transcriptional mechanisms controlling the different expression of apple MdPSY1 and MdPSY2 is supported by the significant sequence differences in their promoters as well as the varied responses to transient activation by the AP2/ERF transcription factors we tested.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
CDS Sequence
  • >MDP0000177623
    ATGTCAGGTGTTCTTCTCTGGGTGGTGAGTCCCAAAGAGAATGCCAGCTCTCCGCTGGGTCTGTTGCCGAGAATTTGCACCCCAAGGAGGTCTAAATTGTGCTCAAAGCTGGGTTTTTCAAGTGGGGTTTTGGCCTACTCGGGTGCAGTTGCAAACCCAGCCAGATCTTCAGAGGAGAAGGTGTATGAAGTGGTGCTGAAGCAGGCTGCTCTAGTGAGAGAACCGAACACGTTGAGTGCACATCTTGAATCGCTTTTTTCTTCGAAACGTTCGCACGCAGCACAATTTGGAATTATTTTTGCTTCCATTTTTTACTTGAAATGGATATTTATGAATTGGAAGGAAACTGTATTTACCAACTTCTTTCTGTGTTCTTCAGGGACATTGCTCATGACACCGGAGCGGCGACRAGCTGTTTGGGCAATCTATGTGTGGTGCAGAAGGACGGATGAGCTAGTGGATGGACCTAATGCTTCATACATTACACCCAAAGCTCTTGACAGATGGGAGAAAAGACTGACAGATCTCTTCGAAGGCCGGCCTTATGATATGTATGATGCTGCTCTATCTGATACAGTCGCCAAGTACCCTGTTGACATTCAGCCCTTCAGAGACATGGTAGAAGGAATGAGATTAGACTTGAGAAAATCAAGATACCAGAACTTTGATGAACTTTACCTTTACTGCTACTATGTTGCTGGAACTGTTGGATTGATGAGTGTTCCGGTAATGGGGATATCCCGGGAATCAAAGGCCTCAACAGAAAGTGTTTACAATGCTGCATTGGCCCTTGGAATTGCTAATCAGCTCACTAACATTCTCAGAGATGTTGGAGAAGATGCTAGGAGAGGAAGGATATATCTCCCACAAGACGAGCTTGCCCAAGCCGGCCTATCAGACGATGACATCTTTCGCGGGAAGGTGACTGACAAGTGGCAAAGTTTCATGAAGGGACAAATAAAGAGAGCTAGGATGTTCTTTGATGAGGCTGAGAAGGGTGTCTCAGAGCTCAACTCAGCTAGTAGATGGCCAGTATGGGCATCTTTGTTGCTGTACAGGCAGATTCTAGATGCAATTGAAGCAAATGGTTATGACAATTTCACAAAAAGGGCATATGTGGGAAAAGCAAAGAAGTTAGCATCATTGCCTGTGGCCTATGGAAGGGCCATTTTAGGACCCTCTAATTTAACTAAGCAGTTGGTGCCTAGATGA
Protein Sequence
  • >MDP0000177623
    MSGVLLWVVSPKENASSPLGLLPRICTPRRSKLCSKLGFSSGVLAYSGAVANPARSSEEKVYEVVLKQAALVREPNTLSAHLESLFSSKRSHAAQFGIIFASIFYLKWIFMNWKETVFTNFFLCSSGTLLMTPERRXAVWAIYVWCRRTDELVDGPNASYITPKALDRWEKRLTDLFEGRPYDMYDAALSDTVAKYPVDIQPFRDMVEGMRLDLRKSRYQNFDELYLYCYYVAGTVGLMSVPVMGISRESKASTESVYNAALALGIANQLTNILRDVGEDARRGRIYLPQDELAQAGLSDDDIFRGKVTDKWQSFMKGQIKRARMFFDEAEKGVSELNSASRWPVWASLLLYRQILDAIEANGYDNFTKRAYVGKAKKLASLPVAYGRAILGPSNLTKQLVPR