Gene Details:

Functional Descriptions:

  • Apple MdABCF assists in the transportation of S-RNase into pollen tubes.
  • We show here that the apple (Malus domestica) MdABCF protein, which belongs to group F of the ABC transporter family, assists in transportation of S-RNase into the pollen tube.
  • MdABCF is located in the pollen tube membrane and interacts with S-RNase. S-RNase was unable to enter the pollen tube when MdABCF was silenced by antisense oligonucleotide transfection.
  • Our results show that MdABCF assists in transportation of either self or non-self S-RNase into the pollen tube. Moreover, MdABCF coordinates with the cytoskeleton to transport S-RNase. Blockage of S-RNase transport disrupts self-incompatibility in this system.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
CDS Sequence
  • >MDP0000170302
    ATGACGGTGGCTAGCTCAGTGGTCCACGAAGTGCTGGGCCGGCGGGCCGAGGACGTCGATGAGCCCATAATCGAGTACATCGTTAACGTCCTCGCCGACGAGGACTTCGATTTCGGCGACGACGGTGAAGGCGCCTTCGACGCTCTCGGCGAGCTCCTCGTCGGAGCCGGCTGTGTTAGCGACTTCGCTGAGTGCCGCTCGGTTTGCAGTATAATATCCGAGAAGTTCGGGAAACATGGGTTGGTTAAAGCTAAACCGACTGTGCGGAGCCTATCAGCACCAGTAAGAATGGATGATGGGATGGATGAGAAGGTAGCTCCAAAGAAGAAGGTTGAGGTGATTGATGGCCCTCTGTTAACTGAACGTGACCGAGCTAAGATAGAGAGGAAAAAGAGGAAGGATGATCGCCAAAGAGAGCAACAATATCAAATACATTTAGCAGAGATGGAAGCAGTCAGGGCAGGAATGCCTGTTGTATCCGTAAATCATGAAAGTGCTGGTGGCCCAAATGTCAGGGATATCCGTCTGGAAAACTTCAATGTTTCTGTGGGTGGCCGTGATCTAATTGTGGATGGTTCAGTAACACTTTCTTTTGGAAGGCATTATGGCCTTGTTGGAAGAAATGGTACGGGGAAAACAACGTTTCTTAGGCACTTGGCTATGCATGCCATTGACGGCATTCCTAGGAACTTCCAAATATTGCATGTGGAGCAAGAAGTGGTGGGTGATGATACAACAGCCTTGCAGTGTGTTCTTAACACTGATGTTGAAAGAACAAAGCTTTTGGAAGAAGAAGCTCGTCTTCTCACCCAACAGAGAGCATTGGAGTTTGAGGACTCAACAGAAAAGAGTAACGGGGAAGTTGATAAAGATGCCATTGGGCAGAGGCTTCAGGAGATATACAAAAGGCTCGAGTTCATTGATGCTGATTCGGCAGAGTCACGGGCAGCTTCCATTCTTGCGGGTCTTAGTTTCTCTCCGGAGATGCAGTTGAAACCAACTAAAGCATTTTCAGGAGGATGGCGGATGCGAATAGCTCTTGCTCGTGCCCTGTTTATAGAGCCTGATTTGTTACTGCTTGATGAACCCACGAACCATCTTGATCTTCATGCAGTCCTCTGGCTAGAGGCTTACCTGGTGAAGTGGCCAAAAACATGCATAGTTGTTTCTCATGCTAGAGAATTCTTGAACACGGTAGTCACTGATATACTACATCTTCATGGGCAAAAATTGAATGCTTACAAGGGAGACTACGATACATATGAGAGGACGCGGATAGAACTAGTCAAGAACCAACAGAAGGCTTTTGAGGCCAATGAGCGTTCAAGAACACATATGCAGTCGTTTATTGATAAGTTCCGCTACAATGCCAAGAGAGCTGCGCTTGTTCAGTCAAGAATTAAGGCTTTGGACCGGTTGGGACATGTGGATGAGATTGTAAATGACCCTGACTATAAGTTTGAGTTCCCAACTCCAGACGACAGACCTGGTCCTCCAATAATAAGTTTCAGTGATGCATCATTTGGTTATCCTGGGGGACCCGTCTTGTTTAGGAATTTAAATTTTGGAATTGACCTTGACAGCCGCATCGCAATGGTCGGGCCAAATGGTATTGGTAAATCAACGATACTCAAACTAATTGCAGGGGAGCTACAACCAATCTCTGGGACAGTTTTTCGTTCAGCTAAGGTTCGGATCGCTGTATTCAGTCAGCACCATGTTGACGGACTAGATTTATCTTCTAATCCCCTATTGTATATGATGCGCTGCTTCCCGGGGGTGCCTGAACAAAAGCTCCGATCTCACTTAGGTTCTTTCGGTGTCAGTGGAAATCTTGCACTTCAGCCTATGTACACATTGTCTGGTGGTCAGAAGAGCAGAGTTGCATTTGCCAAGATAACTTTCAAGAAACCGCACATAATACTGCTTGATGAGCCCTCCAATCATCTGGACTTGGATGCTGTGGAGGCACTAATTCAAGGTCTTGTTCTCTTCCAAGGAGGCATTCTCATGGTTAGTCACGACGAGCATCTGATCTCTGGAAGTGTGGACGAGCTGTGGGTGGTATCAGAAGGTAGGATACAACCATTCCATGGCAGTTTTGAGGACTACAAGAAGATACTGCAGTCCTCGTAG
Protein Sequence
  • >MDP0000170302
    MTVASSVVHEVLGRRAEDVDEPIIEYIVNVLADEDFDFGDDGEGAFDALGELLVGAGCVSDFAECRSVCSIISEKFGKHGLVKAKPTVRSLSAPVRMDDGMDEKVAPKKKVEVIDGPLLTERDRAKIERKKRKDDRQREQQYQIHLAEMEAVRAGMPVVSVNHESAGGPNVRDIRLENFNVSVGGRDLIVDGSVTLSFGRHYGLVGRNGTGKTTFLRHLAMHAIDGIPRNFQILHVEQEVVGDDTTALQCVLNTDVERTKLLEEEARLLTQQRALEFEDSTEKSNGEVDKDAIGQRLQEIYKRLEFIDADSAESRAASILAGLSFSPEMQLKPTKAFSGGWRMRIALARALFIEPDLLLLDEPTNHLDLHAVLWLEAYLVKWPKTCIVVSHAREFLNTVVTDILHLHGQKLNAYKGDYDTYERTRIELVKNQQKAFEANERSRTHMQSFIDKFRYNAKRAALVQSRIKALDRLGHVDEIVNDPDYKFEFPTPDDRPGPPIISFSDASFGYPGGPVLFRNLNFGIDLDSRIAMVGPNGIGKSTILKLIAGELQPISGTVFRSAKVRIAVFSQHHVDGLDLSSNPLLYMMRCFPGVPEQKLRSHLGSFGVSGNLALQPMYTLSGGQKSRVAFAKITFKKPHIILLDEPSNHLDLDAVEALIQGLVLFQGGILMVSHDEHLISGSVDELWVVSEGRIQPFHGSFEDYKKILQSS