Gene Details:

• Gene ID: MD16G1175800
• Gene Symbol: MdNHX2
• Gene Name:
• Genome: Golden ASM211411v1
• Species: Malus domestica

Functional Descriptions:

• MdNHX2 improves KCl tolerance in apple plants by compartmentalizing K+.
• Our results indicate that MT alleviates KCl stress by inducing K+ efflux outside of the cell and compartmentalizing K+ into vacuoles by controlling the transcriptional regulation of MdGORK1 and MdNHX2 via the transcription factor MdWRKY53 in apple.
• RT–qPCR and LUC activity analyses indicated that MdWRKY53 binds to the promoters of MdGORK1 and MdNHX2 and induces their transcription.
• Overexpressing MdWRKY53 enhanced KCl tolerance in transgenic apple plants by increasing K+ efflux and K+ compartmentalization. Subsequently, we characterized the transporter genes MdGORK1 and MdNHX2 as downstream targets of MdWRKY53 by ChIP-seq. MdGORK1 localized to the plasma membrane and enhanced K+ efflux to increase KCl tolerance in transgenic apple plants. Moreover, overexpressing MdNHX2 enhanced the KCl tolerance of transgenic apple plants/callus by compartmentalizing K+ into the vacuole. RT–qPCR and LUC activity analyses indicated that MdWRKY53 binds to the promoters of MdGORK1 and MdNHX2 and induces their transcription.

Function-related keywords:

• transcription-factor ,  stress ,  tolerance ,  stress-tolerance ,  transporter ,  plasma-membrane ,  callus

Literature:

• Melatonin enhances KCl salinity tolerance by maintaining K(+) homeostasis in Malus hupehensis.   DOI:  10.1111/pbi.14129 ;  PMID:  37465981

Related News:

• PBJ | 青岛农大王彩虹/郑晓东课题组揭示苹果通过维持钾离子稳态应答盐碱胁迫的新机制

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: