Gene Details:
- Gene ID: MD14G1239500
- Gene Symbol: MdGORK1
- Gene Name:
- Genome: Golden ASM211411v1
- Species: Malus domestica
Functional Descriptions:
- We characterized the transporter genes MdGORK1 and MdNHX2 as downstream targets of MdWRKY53 by ChIP-seq.
- MdGORK1 localized to the plasma membrane and enhanced K+ efflux to increase KCl tolerance in transgenic apple plants.
- RT–qPCR and LUC activity analyses indicated that MdWRKY53 binds to the promoters of MdGORK1 and MdNHX2 and induces their transcription.
- Overexpressing MdWRKY53 enhanced KCl tolerance in transgenic apple plants by increasing K+ efflux and K+ compartmentalization. Subsequently, we characterized the transporter genes MdGORK1 and MdNHX2 as downstream targets of MdWRKY53 by ChIP-seq. MdGORK1 localized to the plasma membrane and enhanced K+ efflux to increase KCl tolerance in transgenic apple plants. Moreover, overexpressing MdNHX2 enhanced the KCl tolerance of transgenic apple plants/callus by compartmentalizing K+ into the vacuole. RT–qPCR and LUC activity analyses indicated that MdWRKY53 binds to the promoters of MdGORK1 and MdNHX2 and induces their transcription.
Function-related keywords:
Literature:
- Melatonin enhances KCl salinity tolerance by maintaining K(+) homeostasis in Malus hupehensis. DOI: 10.1111/pbi.14129 ; PMID: 37465981
Related News:
Gene Resources:
Sequences:
cDNA Sequence
mRNA Sequence
- />MD14G1239500
CCACAAAAAGTTACTGTTTATTACTTGGTATCAAACAACAACAGTATCATTGGCATTAAATATACATTGGCTTTGGCTCTCATTTAACTTTATTCACAAGTTTGTTATTATTGAAAGAGGTCGATCTGAGGAAGATAAAAAATGCATGGAGCTGAAAGGAGAAAATCGTCAGGAGGAGGTGCTCATGATCAGTACGAAGATGAGGGGTCGACGTTTGAGGATGATGATGATTATGAGGAGGAGCATATTAATGATGATTCTAAGAAACAGTGGCTGTGGAAATTAGGGCGTTTTATCGCTTGTACGACTGCCGGCGACTCCACCACTCTTACTACTACTCCTGGCAGTACAAGTACTGGAAGCAGATCATCGTCTGCATCTTCGTTTCTTGATCGACTCGTTATTCATCCTGATAACTGGTTGTACGTGGGGTGGACGCATTTCATTCTGCTATGGGCAGTCTATTCGTCCTTCTTCACGCCATTGGAGTTCGGCTTCTTCCGGGGTCTGCCCGAGAATCTGTTCCTGCTCGACATCGCCGGCCAGCTGGCCTTCCTTGTCGACATCGTCGTACGCTTCTTCGTGGCCTACCGAGACTTTCACTCGCACCGTATTGTCTACAACCGCAACCTCATCGCTCTCCGCTACTTGAAGTCTCGATTTCTCGTTGATTTGCTCGGTTGTCTGCCTTGGGACGCCATCTACAAGGCTTCTGGGAAAGAAGAGGGAGTGAGGTACCTGTTATGGATAAGACTAAGTCGAGCACGCAGAGTAACAGAGTTTTTTGAGAAGTTGGAGAAAGATATAAGGATCAACTACCTTTTTACAAGAATTGCCAAACTTTTTGTTGTGGAACTTTATAGCACACACACGGCTGCCTGCATCTTTTATTACCTTGCTACTACCATACCTCCGTCACGGGAAGGATACACATGGATTGGAAGTTTGAAGATGGGTGACTATAGCTATTCCCATTTTAGAGAAATTGACCTTTGGAAGCGTTACGTTACCTCACTCTATTTCGCCGTTGTTACCATGGCAACCGTTGGATATGGAGAGATACATGCAGTGAATGTGAGGGAAATGATATTCATCATGTTTTATGTGTCTTTTGACATGCTTTTGGGTGCTTATCTGCTGGGTAACATTACCGCACTGATTGTAAAAGGATCAAAAACTGAAAAGTTTAGGGACAAGATGGCCGAACTTATAAAATACATGAAGAAAAACAGGCTTGATAGGGGGATAAGTAAGGATATCAAAAATCATTTGAGGTTACAGTATGATCGTAGCTACACCGAAGCGGCTGTTCTTCAGGATATTCCAGCCTCCATTCGCAGCAAGATTTCACAGAAATTATATGAACCATACATCAAAGAAGTTTCTCTTTTTAAGGGATGCTCTTTAGGATTCATAAAGCAGATTGCAACCAAAATTCATGAGGAGTTTTTCCTTCCAGGAGAAGTGATCATAGAGCAGGGACAAGCAGTGGATCAATTATACATAGTATGCCATGGGGAGCTGGAGAAGGTGAGAAGGGATGAAGACAGTGAAAGTGAAGAGCTCCTTGAGCGCCTGCAAACCTATAGCTCGTTTGGCGAGGTTTCTTTTCTTTGTAATACTCCCCAGCCATATTCTGTTCGGGCTAGTCAACTGTGCAGGGTCTTGCGACTTGATAAACAATCTTTTAGGGAAATCCTGGATATACATTTCTTAGATGGGCGGATTATCCTAAACAACCTCCTCGAGGGAAAAGACGTGAATACCCGGAACAAGCTTTTGGAATCAGATATCATCCTATATATTGGAAAGCATGAAATGGAGTTAGTTATGAAAGTAAATTGTGCTGCCTATGATGGAGATTTTTATCGAGTAAAACGTTTGATTGGAGCTGGAGCTGATCCCAACAAGATGGACTATGACGGAAGGTCGCCTTTGCATGTTGCTGCATCAAAAGGGTTTGTTGATATCACTCGTTTCCTTATTGAACAAGGAGCGGCCGCTGAGATGTCAGATAAATATGGGAACACTCCTTTACTTGAAGCCATTAAAAATGGGCACGACGAAGTAGGTTCTTTACTTGTTAAGGCGGGGGCATCACTCGCTGTTGATGATGCCGGTGATTTTCTCTGTACCACGGCAGCAAGGAGGAATATGGAGCTTCTGAAAAGGCTTTTATCCAATGACATAAACCCGAATGCGAAAAATTATGATCAGCGGTCACCACTTCATGTAGCTGCTTCAGAAGGCTTATATCCAATGGCAGAATTCCTTTTAGAAGCAGGAGCAAGTGTTCTATCAAAGGACAGATGGGGAAGAACTCCACTTGATGAAGCCCGCATAGGTGGAAATAAGAAACTGATCAAATTGCTTGAAGTCGCCAGAGCTTCTCAGTTGTCAGATTTCTCCGATCATTCTCAAGAAACTGGTTAACATTAATTTTTATACATTTCTCGAACATGTAGTACACATTTTATTACTAGCTGCTAGCCAATCTGTATTATCTGTAATATTACATTATCCTTTGAATTCATATCGAAGTAAGCTACTCTAATGAACTTAGTACCAGTTTGGCTTAAGCTTAAATTATAAAAAAAGTGGGTACCAAAAAAAGTT
Protein Sequence
- />MD14G1239500
MHGAERRKSSGGGAHDQYEDEGSTFEDDDDYEEEHINDDSKKQWLWKLGRFIACTTAGDSTTLTTTPGSTSTGSRSSSASSFLDRLVIHPDNWLYVGWTHFILLWAVYSSFFTPLEFGFFRGLPENLFLLDIAGQLAFLVDIVVRFFVAYRDFHSHRIVYNRNLIALRYLKSRFLVDLLGCLPWDAIYKASGKEEGVRYLLWIRLSRARRVTEFFEKLEKDIRINYLFTRIAKLFVVELYSTHTAACIFYYLATTIPPSREGYTWIGSLKMGDYSYSHFREIDLWKRYVTSLYFAVVTMATVGYGEIHAVNVREMIFIMFYVSFDMLLGAYLLGNITALIVKGSKTEKFRDKMAELIKYMKKNRLDRGISKDIKNHLRLQYDRSYTEAAVLQDIPASIRSKISQKLYEPYIKEVSLFKGCSLGFIKQIATKIHEEFFLPGEVIIEQGQAVDQLYIVCHGELEKVRRDEDSESEELLERLQTYSSFGEVSFLCNTPQPYSVRASQLCRVLRLDKQSFREILDIHFLDGRIILNNLLEGKDVNTRNKLLESDIILYIGKHEMELVMKVNCAAYDGDFYRVKRLIGAGADPNKMDYDGRSPLHVAASKGFVDITRFLIEQGAAAEMSDKYGNTPLLEAIKNGHDEVGSLLVKAGASLAVDDAGDFLCTTAARRNMELLKRLLSNDINPNAKNYDQRSPLHVAASEGLYPMAEFLLEAGASVLSKDRWGRTPLDEARIGGNKKLIKLLEVARASQLSDFSDHSQETG