Gene Details:

Functional Descriptions:

  • An 8-bp deletion in the MdERF3 promoter disrupted MdDOF5.3 binding, reduced MdERF3 expression, relieved the inhibition on MdPGLR3, MdPME2, and MdACO4 expression, and ultimately decreased flesh firmness and crispness retainability.
  • Role of MdERF3 and MdERF118 natural variations in apple flesh firmness/crispness retainability and development of QTL-based genomics-assisted prediction.
  • MdERF3 was then transiently overexpressed via Agrobacterium-mediated infiltration or silenced via pTRV virus-induced gene silencing (VIGS).
  • These data further confirmed that MdERF3 contributed to post-harvest flesh firmness/crispness retainability.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
mRNA Sequence
  • />MD03G1194300
    ATGAGGAGGGGCAGAGGAGCAGCCACGAGAGCCGCAGCGCCGCCGGCCGAACCCACCGGATCCAGACGCAGTATTGGCAAAGAGGTCCGGTACCGAGGGGTGCGGAGAAGACCGTGGGGTCGGTTTGCAGCCGAGATCCGGGACCCGTGGAAGAAGACCCGGGTTTGGCTCGGCACCTTCGACTCGGCCGAAGACGCCGCCCGAGCTTACGATGCCGCCGCTCGGATCCTCCGCGGCCCCAAGGCCAAGACTAACTTCCCGATCTCATGTGCTTACCCGCCCTTTTACAACAACCCTACCGACACTATCGAGGCAAAGCTTTTCGGGTCGGGTTACGGATTTCAGGACAATCCGGTTGTAGATCCTCAGAGACCCGCATCCAGCAGCCTGAGCTCCACCGTGGAGTCGTTCAGTGGGCCCCGGCCGCCGCCTCAATTTACGACGATGGCTGCCGAGTTGTCGCGGCCTAAACGACAGCCGAGGACCCCGCCGGTGGTTCCCGAGGACTGCCACAGCGACTGCGACTCGTCGTCATCGGTGGTTGAGGACGACGGGGATTTCGTTGCGTCGTCGTCGTTTCGCAAGGCCCCGCTGCCCTTCGATCTTAATTTCCCACCGTTGGATGTGGATGGGGATTTGGGCGGTGGGGTGGACGGTCATGATCTTCGATGTACGGCTCTATGCCTTTGA
Protein Sequence
  • />MD03G1194300
    MRRGRGAATRAAAPPAEPTGSRRSIGKEVRYRGVRRRPWGRFAAEIRDPWKKTRVWLGTFDSAEDAARAYDAAARILRGPKAKTNFPISCAYPPFYNNPTDTIEAKLFGSGYGFQDNPVVDPQRPASSSLSSTVESFSGPRPPPQFTTMAAELSRPKRQPRTPPVVPEDCHSDCDSSSSVVEDDGDFVASSSFRKAPLPFDLNFPPLDVDGDLGGGVDGHDLRCTALCL