Gene Details:

  • Gene ID:
  • Gene Symbol: MdCRF4
  • Gene Name: Malus domestica CYTOKININ RESPONSE FACTOR4
  • Genome: Golden ASM211411v1
  • Species: Malus domestica

Functional Descriptions:

  • Phosphorylation of MdCYTOKININ RESPONSE FACTOR4 suppresses ethylene biosynthesis during apple fruit ripening.
  • A transcriptional activator of ethylene biosynthesis- and signaling-related genes, including Md1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID SYNTHASE1 (MdACS1) and MdETHYLENE-RESPONSIVE FACTOR3 (MdERF3), as a partner of the calcium sensor, calmodulin.
  • Ca2+ promoted the Ca2+/CaM2-mediated phosphorylation of MdCRF4, resulting in MdCRF4 recognition by the E3 ubiquitin ligase MdXB3 ORTHOLOG 1 IN ARABIDOPSIS THALIANA (MdXBAT31), and consequently its ubiquitination and degradation via the 26S proteasome pathway.
  • Transiently overexpressing various MdCRF4 proteins with specific mutated phosphorylation sites revealed that the phosphorylation state of MdCRF4 affects the ripening of apple fruit.
  • The results reveal that a Ca2+/CaM-MdCRF4–MdXBAT31 module is involved in Ca2+-suppressed ethylene biosynthesis, which delays apple fruit ripening.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
CDS Sequence
Protein Sequence