Gene Details:

Functional Descriptions:

  • In vitro and in vivo assays indicate that MdBT2 interacts directly with and ubiquitinates a bHLH transcription factor, MdCIbHLH1, via the ubiquitin/26S proteasome pathway in response to nitrate.
  • This ubiquitination results in the degradation of MdCIbHLH1 protein and reduces the transcription of MdCIbHLH1-targeted genes involved in malate accumulation and vacuolar acidification, including MdVHA-A, which encodes a vacuolar H+-ATPase, and MdVHP1, which encodes a vacuolar H+-pyrophosphatase, as well as MdALMT9, which encodes an aluminum-activated malate transporter.
  • Taken together, these findings reveal that MdBT2 regulates the stability of MdCIbHLH1 via ubiquitination in response to nitrate, which in succession transcriptionally reduces the expression of malate-associated genes, thereby controlling malate accumulation and vacuolar acidification in apples under high nitrate supply.
  • MdBT2 regulates the stability of MdCIbHLH1 via ubiquitination in response to nitrate, which in succession transcriptionally reduces the expression of malate-associated genes, thereby controlling malate accumulation and vacuolar acidification in apples under high nitrate supply.
  • The cold-induced basic helix-loop-helix transcription factor gene MdCIbHLH1encodes an ICE-like protein in apple.
  • A apple bHLH (basic helix-loop-helix) gene MdCIbHLH1 (Cold-Induced bHLH1), which encodes an ICE-like protein, was noticeably induced in response to cold stress.
  • Finally, we observed that cold induces the degradation of the MdCIbHLH1 protein in apple and that this degradation was potentially mediated by ubiquitination and sumoylation.
  • Our findings indicate that MdCIbHLH1 functions in stress tolerance in different species. For example, ectopic MdCIbHLH1 expression conferred enhanced chilling tolerance in transgenic tobacco.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
CDS Sequence
  • >MDP0000662999
    ATGCTGCCAAGGCTGAACGGTGGTGTTTGGATGGACGACAGGGAGGACAGAGACTCTGTTTCCTGGACTAGAACCTCCGCCACCGCCACCGCCGGCAACACCGAGAACAAGGACGAGATGGGTTCTTCTCTGTCTACCTTCAARTCGATGCTGGAGGTTGAAGACGACTGGTACATGGCGGCGAATAACAGCATCCAGGGCCATTCAGATGTCGGAGACATCAGCTTCTCGCCGAGCTTTGCCGACCCAGAAAGCTTGCTGCTCAACCCGGTGGACTCTTCCTCCTCCTGCTCACCGTCGTCGTCGGTTTTCAACAATCTCGACCCGAATCAGGTTCATTACTACATGCCTCAAAATCCCAACTTGTCTTCGCTGCTTAATGTTGTTCCTAACAACCCTTTGGATCACGGCTTCGATTTGGGCTGTGACATTGGGTTTCTTGACACTCAAGCTTCGGGTGGCGGTTCGAGTATGATGAACAGGGGAGGTGGGGTTTTATCTGGATTCAATGATTTAAGCTCGAATAGCCAGGTGAATGCTCAGAATTTGGGTTCTAATTTACAGTTTTCGACTACCCGCATGCCTCAAGCTCTTGAAAACAGTTCAAATTTTTCGGGTTTTCGAGGTGTGGATGATGGTTCGGCAAATGCTTTGTTTCCCAATAGGCCTAAGTTACTGAGACCACTTGAATCCTTCCCTTCTGTGGGAGTCCAGCCAACTCTTTTCCAGAAAAGAGCAGCGCTTCGGAAAAACTTAGGCGATGGTGGAGGGAATTTGGGTGTTTTGGKCTCACAAGGGGGCCTGGTTTTGAATGAAGGGGATGAGAGGAAGAGGGAGATGGGTGTGCAGAATGAGAAGAAGAGGAAAATGAGCGGCGSMGATGATGTCGATGATCTGAGTTTCGATGGTTCGGGTTTGAACTATGATTCGGATGAGTTTACTGAGAACACTAAGGTTGATGACGGTGCCAAGAATGGTGGGAACAGCTCGAATGCAAATAGCACKGTTACCGGTGGAGGAGGGGGTCATAAGGGGAAGAAGAAAGGGTTGCCTGCCAAGAATCTGATGGCTGAGAGGCGCCGCCGGAAGAAGCTCAATGATAGGCTGTACATGCTGAGATCCGTTGTTCCGAAGATTAGCAAAATGGACAGGGCCTCAATCCTTGGGGATGCAATTGAGTACTTGAAGGAACTTCTGCAGAGGATCAACAACCTCCACAATGAACTGGAGTCAATCCCTCCTGGATCTGCATTGACACCTACCGGAAATACTTTCCACCCTTTGACACCCACTCCGGCTACTCTGCCAAATCGTATCAAGGAAGAACTTTGCCCCAGCTCATTACCTAGCCCAAATGGCCAAGCTGCAAGGGTTGAAGTTAGGCTGCGAGAAGGACGAGCAGTTAACATCCACATGTTCTGCGGCCGAAGGCCAGGTCTCTTGCTCTCAACCATGAGAACTTTGGATAACCTTGGGTTAGACATCCAGCAAGCCGTCATCAGCTGTTTCAATGGTTTTGCTATGGATGTCTTCCGTGCCGAGCAATGCAAGGAAGGCCAAGACGTCCACCCGGATCAGATAAAGGCGGTACTTCTGGATTCGATCGGGTTCCATGGCATGATGTAG
Protein Sequence
  • >MDP0000662999
    MLPRLNGGVWMDDREDRDSVSWTRTSATATAGNTENKDEMGSSLSTFKSMLEVEDDWYMAANNSIQGHSDVGDISFSPSFADPESLLLNPVDSSSSCSPSSSVFNNLDPNQVHYYMPQNPNLSSLLNVVPNNPLDHGFDLGCDIGFLDTQASGGGSSMMNRGGGVLSGFNDLSSNSQVNAQNLGSNLQFSTTRMPQALENSSNFSGFRGVDDGSANALFPNRPKLLRPLESFPSVGVQPTLFQKRAALRKNLGDGGGNLGVLXSQGGLVLNEGDERKREMGVQNEKKRKMSGXDDVDDLSFDGSGLNYDSDEFTENTKVDDGAKNGGNSSNANSTVTGGGGGHKGKKKGLPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIEYLKELLQRINNLHNELESIPPGSALTPTGNTFHPLTPTPATLPNRIKEELCPSSLPSPNGQAARVEVRLREGRAVNIHMFCGRRPGLLLSTMRTLDNLGLDIQQAVISCFNGFAMDVFRAEQCKEGQDVHPDQIKAVLLDSIGFHGMM*