Information report for MdCIbHLH1
Gene Details
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Functional Descriptions
- In vitro and in vivo assays indicate that MdBT2 interacts directly with and ubiquitinates a bHLH transcription factor, MdCIbHLH1, via the ubiquitin/26S proteasome pathway in response to nitrate.
- This ubiquitination results in the degradation of MdCIbHLH1 protein and reduces the transcription of MdCIbHLH1-targeted genes involved in malate accumulation and vacuolar acidification, including MdVHA-A, which encodes a vacuolar H+-ATPase, and MdVHP1, which encodes a vacuolar H+-pyrophosphatase, as well as MdALMT9, which encodes an aluminum-activated malate transporter.
- Taken together, these findings reveal that MdBT2 regulates the stability of MdCIbHLH1 via ubiquitination in response to nitrate, which in succession transcriptionally reduces the expression of malate-associated genes, thereby controlling malate accumulation and vacuolar acidification in apples under high nitrate supply.
- MdBT2 regulates the stability of MdCIbHLH1 via ubiquitination in response to nitrate, which in succession transcriptionally reduces the expression of malate-associated genes, thereby controlling malate accumulation and vacuolar acidification in apples under high nitrate supply.
- The cold-induced basic helix-loop-helix transcription factor gene MdCIbHLH1encodes an ICE-like protein in apple.
- A apple bHLH (basic helix-loop-helix) gene MdCIbHLH1 (Cold-Induced bHLH1), which encodes an ICE-like protein, was noticeably induced in response to cold stress.
- Finally, we observed that cold induces the degradation of the MdCIbHLH1 protein in apple and that this degradation was potentially mediated by ubiquitination and sumoylation.
- Our findings indicate that MdCIbHLH1 functions in stress tolerance in different species. For example, ectopic MdCIbHLH1 expression conferred enhanced chilling tolerance in transgenic tobacco.
Functional Keywords
- transcription-factor , atpase , transporter , aluminum , nitrate , ubiquitin , nitrate-transporter , stress , tolerance , cold-tolerance , cold-stress , cold , stress-tolerance , chilling , chilling-stress , chilling-tolerance , stress-response , protein
Literature and News
- BTB-TAZ Domain Protein MdBT2 Modulates Malate Accumulation and Vacuolar Acidification in Response to Nitrate. DOI: 10.1104/pp.20.00208 ; PMID: 32241879
- The cold-induced basic helix-loop-helix transcription factor gene MdCIbHLH1 encodes an ICE-like protein in apple. DOI: 10.1186/1471-2229-12-22 ; PMID: 22336381
Sequences
cDNA Sequence
CDS Sequence
- >MDP0000662999
ATGCTGCCAAGGCTGAACGGTGGTGTTTGGATGGACGACAGGGAGGACAGAGACTCTGTTTCCTGGACTAGAACCTCCGCCACCGCCACCGCCGGCAACACCGAGAACAAGGACGAGATGGGTTCTTCTCTGTCTACCTTCAARTCGATGCTGGAGGTTGAAGACGACTGGTACATGGCGGCGAATAACAGCATCCAGGGCCATTCAGATGTCGGAGACATCAGCTTCTCGCCGAGCTTTGCCGACCCAGAAAGCTTGCTGCTCAACCCGGTGGACTCTTCCTCCTCCTGCTCACCGTCGTCGTCGGTTTTCAACAATCTCGACCCGAATCAGGTTCATTACTACATGCCTCAAAATCCCAACTTGTCTTCGCTGCTTAATGTTGTTCCTAACAACCCTTTGGATCACGGCTTCGATTTGGGCTGTGACATTGGGTTTCTTGACACTCAAGCTTCGGGTGGCGGTTCGAGTATGATGAACAGGGGAGGTGGGGTTTTATCTGGATTCAATGATTTAAGCTCGAATAGCCAGGTGAATGCTCAGAATTTGGGTTCTAATTTACAGTTTTCGACTACCCGCATGCCTCAAGCTCTTGAAAACAGTTCAAATTTTTCGGGTTTTCGAGGTGTGGATGATGGTTCGGCAAATGCTTTGTTTCCCAATAGGCCTAAGTTACTGAGACCACTTGAATCCTTCCCTTCTGTGGGAGTCCAGCCAACTCTTTTCCAGAAAAGAGCAGCGCTTCGGAAAAACTTAGGCGATGGTGGAGGGAATTTGGGTGTTTTGGKCTCACAAGGGGGCCTGGTTTTGAATGAAGGGGATGAGAGGAAGAGGGAGATGGGTGTGCAGAATGAGAAGAAGAGGAAAATGAGCGGCGSMGATGATGTCGATGATCTGAGTTTCGATGGTTCGGGTTTGAACTATGATTCGGATGAGTTTACTGAGAACACTAAGGTTGATGACGGTGCCAAGAATGGTGGGAACAGCTCGAATGCAAATAGCACKGTTACCGGTGGAGGAGGGGGTCATAAGGGGAAGAAGAAAGGGTTGCCTGCCAAGAATCTGATGGCTGAGAGGCGCCGCCGGAAGAAGCTCAATGATAGGCTGTACATGCTGAGATCCGTTGTTCCGAAGATTAGCAAAATGGACAGGGCCTCAATCCTTGGGGATGCAATTGAGTACTTGAAGGAACTTCTGCAGAGGATCAACAACCTCCACAATGAACTGGAGTCAATCCCTCCTGGATCTGCATTGACACCTACCGGAAATACTTTCCACCCTTTGACACCCACTCCGGCTACTCTGCCAAATCGTATCAAGGAAGAACTTTGCCCCAGCTCATTACCTAGCCCAAATGGCCAAGCTGCAAGGGTTGAAGTTAGGCTGCGAGAAGGACGAGCAGTTAACATCCACATGTTCTGCGGCCGAAGGCCAGGTCTCTTGCTCTCAACCATGAGAACTTTGGATAACCTTGGGTTAGACATCCAGCAAGCCGTCATCAGCTGTTTCAATGGTTTTGCTATGGATGTCTTCCGTGCCGAGCAATGCAAGGAAGGCCAAGACGTCCACCCGGATCAGATAAAGGCGGTACTTCTGGATTCGATCGGGTTCCATGGCATGATGTAG
Protein Sequence
- >MDP0000662999
MLPRLNGGVWMDDREDRDSVSWTRTSATATAGNTENKDEMGSSLSTFKSMLEVEDDWYMAANNSIQGHSDVGDISFSPSFADPESLLLNPVDSSSSCSPSSSVFNNLDPNQVHYYMPQNPNLSSLLNVVPNNPLDHGFDLGCDIGFLDTQASGGGSSMMNRGGGVLSGFNDLSSNSQVNAQNLGSNLQFSTTRMPQALENSSNFSGFRGVDDGSANALFPNRPKLLRPLESFPSVGVQPTLFQKRAALRKNLGDGGGNLGVLXSQGGLVLNEGDERKREMGVQNEKKRKMSGXDDVDDLSFDGSGLNYDSDEFTENTKVDDGAKNGGNSSNANSTVTGGGGGHKGKKKGLPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIEYLKELLQRINNLHNELESIPPGSALTPTGNTFHPLTPTPATLPNRIKEELCPSSLPSPNGQAARVEVRLREGRAVNIHMFCGRRPGLLLSTMRTLDNLGLDIQQAVISCFNGFAMDVFRAEQCKEGQDVHPDQIKAVLLDSIGFHGMM*