Gene Details:

• Gene ID: MD04G0113100
• Gene Symbol: MdLAC7
• Gene Name: laccase 7
• Genome: Golden ASM211411v1
• Species: Malus domestica

Functional Descriptions:

• The detailed analysis identified MdLAC7’s (laccase 7) role in the preharvest apple peel browning process.
• Transient injection, overexpression, and CRISPR/Cas9 knockout of the MdLAC7 gene in apple fruit and calli identified vallinic acid, anthocyanidin, tannic acid, sinapic acid, and catechinic acid as its catalytic substrates. In addition, yeast one-hybrid assay, electrophoretic mobility shift assay, luciferase reporter assay, and ChIP-PCR analysis revealed that MdWRKY31 binds to the promoter of MdLAC7 and positively regulates its activity to promote peel browning of bagged fruits (dark conditions).
• The expression of MdLAC7 in the BF stalk cavity was detected initially at 165 DAFB, which then increased with the intensification of browning.
• The study revealed the MdLAC7-mediated mechanism regulating preharvest apple peel browning and demonstrated the role of light in inhibiting MdLAC7 activity and subsequently reducing peel browning. These results provide theoretical guidance for producing high-quality apple fruits.

Function-related keywords:

• quality ,  calli ,  peel

Literature:

• Transcriptomic and metabolomic analysis reveals a protein module involved in preharvest apple peel browning.   DOI:  10.1093/plphys/kiad064 ;  PMID:  36722358

Related News:

Gene Resources:

• NCBI ID:
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