Gene Details:
- Gene ID:
- Gene Symbol: HvPTR1
- Gene Name: peptide transporter1
- Genome: MorexV3_pseudomolecules_assembly
- Species: Hordeum vulgare
Functional Descriptions:
- A role for phosphorylation in the regulation of the barley scutellar peptide transporter HvPTR1 by amino acids.
- In vivo [32P]orthophosphate labelling studies of barley scutellar tissue in combination with immunoprecipitation studies using antiserum raised to HvPTR1 showed that HvPTR1 (66 kDa) is phosphorylated in the presence of amino acids.
- Immunopurified HvPTR1 was further demonstrated to be phosphorylated on serine residues. Digestion with the N-glycosidase enzyme PNGase F results in a shift in the molecular mass of the protein by 10 kDa, indicating that HvPTR1 is an N-linked glycoprotein.
- These results provide strong circumstantial evidence that HvPTR1 peptide transport activity in the germinating barley grain is regulated at the post-translational level by phosphorylation in response to rising levels of amino acids emanating from the endosperm as a result of storage protein breakdown and mobilization.
Function-related keywords:
- grain , transporter , endosperm , grain-protein , as-transport , glycoprotein , protein
Literature:
- A role for phosphorylation in the regulation of the barley scutellar peptide transporter HvPTR1 by amino acids. DOI: 10.1093/jxb/eri149 ; PMID: 15824072
Related News:
Gene Resources:
- NCBI ID: AV914147.1
- UniProt accessions:
Orthologs:
Sequences:
cDNA Sequence
CDS Sequence
Protein Sequence