Gene Details:

Functional Descriptions:

  • we expressed HvPHT1;6;HvPHT1;6 in Xenopus laevis oocytes, allowing detailed characterization of voltage-dependent fluxes and currents induced by HvPHT1;6;HvPHT1;6. HvPHT1;6;HvPHT1;6 increased efflux of Pi near oocyte resting membrane potentials, dependent on external Pi concentration.
  • Our results demonstrate that HvPHT1;6;HvPHT1;6 is most likely to be a proton-coupled Pi transporter, though it has time-dependent activation at negative membrane potentials with linear concentration dependence similar to voltage-dependent ion channels.
  • The electrophysiological properties of HvPHT1;6;HvPHT1;6, which locates to the plasma membrane when expressed in onion (Allium cepa) epidermal cells, are consistent with its suggested role in the remobilization of Pi in barley plants.
  • The Pi transporter HvPHT1;6;HvPHT1;6 is highly expressed in old leaves compared to young leaves, and its transcript is abundant in phloem tissue, with a lower level also observed in mesophyll cells. These data suggest that HvPHT1;6;HvPHT1;6 plays a major role in Pi remobilization.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >HORVU.MOREX.r3.5HG0523050.1
    CCTATCTCTCTAGCTGCTTCGTCGGCGACGCACAACCAACAGCGATCCCCGGCGGCGGCGAGAGATCAAGGCTCGCGGCCATGGCGCGCGAGCAGCTGGAGGTGCTGTCGGCGCTGGACACGGCCAAGACGCAGTGGTACCACTTCACGGCGATCGTCATCGCCGGCATGGGCTTCTTCACCGACGCGTATGATCTCTTCTGCATCTCGCTCGTCACCAAGCTGCTCGGCCGCATCTACTACTACCGCGAGGGTGCCGACGCCCCCGGCTCGCTGCCGCCCAACGTCGCCGCCGCCGTCAACGGCGTCGCCTTCTGCGGCACGCTCTCGGGCCAGCTCTTCTTCGGCTGGCTCGGCGACCGCATGGGCCGCAAGCGCGTCTACGGCATGACCCTCATGTGCATGGTGCTCTGCTCCATCGCCTCGGGCCTCTCCTTCGGCTCCACCCCCGGCTCCGTCATGGCCACGCTCTGCTTCTTCCGCTTCTGGCTCGGGTTTGGGATCGGCGGCGACTACCCGCTCTCCGCCACCATCATGTCCGAGTACGCCAACAAGAAGACGAGGGGCGCCTTCATCGCCGCCGTATTCGCCATGCAGGGCTTCGGCATCCTCACCGGCGGCGTCGTCACGCTCATCGTGTCCGCCGCGTTCCGCGCCGCCTTCCACGCGCCCGCCTACGAGAAGGGCGCCGTCGCATCCACGCCCCCGCAGGCCGACTTCGTGTGGCGCTTCATCCTCATGTTCGGCGCCGTCCCGGCCCTGCTCACCTACTACTGGCGGATGAAGATGCCCGAGACGGCGCGCTACACGGCGCTCGTCGCCAAGAACGCCAAGCAGGCCGCGGCCGACATGTCCAAGGTGCTCCAGGTGGAGATCGCCGCCGAGGACGAAACCAAGGACAACGACGGGGCCGGGGCCGGCGAAGACCGCAACTCGTTCGGGCTCTTCTCCGGCGAGTTCCTTCGGCGGCACGGGCTCCACCTCCTCGGCACGGCCACCTGCTGGTTCCTCCTCGACATCGCCTTCTACTCGCAGAACCTGTTCCAGAAGGACATCTTCACGGCGATCAACTGGATCCCCAAGGCCAAGACGATGAGCGCCCTCGAAGAAGTGCACCGCATCGCGCGCGCGCAGACGCTCATCGCGCTCTGCGGCACGGTGCCGGGCTACTGGTTCACCGTGGCCCTCATCGACCGGATCGGGCGCTTCTGGATCCAGCTCGGCGGATTCTTCTTCATGGCGGTGTTCATGCTGGGGCTGGCCTTCCCGTACCACCACTGGACGACCCCGGGCAACCACATCGGGTTCGTGGTGCTGTACGCGCTCACCTTCTTCTTCGCCAACTTCGGGCCAAACTCCACCACATTCATCGTGCCGGCAGAGATCTTCCCGGCCAGGCTCCGGTCGACGTGCCACGGCATCTCCGCCGCCGCCGGGAAGCTGGGCGCCATCGTGGGGTCGTTCGGGTTCCTGTACCTGGCGCAGAACCAGGACCCCAGCAAGGTGGACCACGGGTACAAGGCCGGCATCGGGGTCAAGAACTCGCTATTCATCCTCGCCGCCTGCAACTTCCTCGGCATGGCCTTCACCTTCTGCGCGCCCGAGTCCAACGGCATCTCGCTCGAGGAGCTCTCCGGCGAGAACGACGACGAGGCGCCGGCGCCGGCGACGCACGCCAGGACGGTGCCCGTGTGAGACGTCGCCGTACGTCAGATATACCAGTATATGCTACCGGTGGTATACTCTGCAGGTTTGGATGGATGTATGGGTGTTTTCTTTTTCTTGGTTGGGACGTTCAACTCAGGAGTCGAGTACAACAACAATACTACTATTGTCATGTGTCAGAGTTCTCCATGTTAATTAATTAATTAGAG
CDS Sequence
  • >HORVU.MOREX.r3.5HG0523050.1
    ATGGCGCGCGAGCAGCTGGAGGTGCTGTCGGCGCTGGACACGGCCAAGACGCAGTGGTACCACTTCACGGCGATCGTCATCGCCGGCATGGGCTTCTTCACCGACGCGTATGATCTCTTCTGCATCTCGCTCGTCACCAAGCTGCTCGGCCGCATCTACTACTACCGCGAGGGTGCCGACGCCCCCGGCTCGCTGCCGCCCAACGTCGCCGCCGCCGTCAACGGCGTCGCCTTCTGCGGCACGCTCTCGGGCCAGCTCTTCTTCGGCTGGCTCGGCGACCGCATGGGCCGCAAGCGCGTCTACGGCATGACCCTCATGTGCATGGTGCTCTGCTCCATCGCCTCGGGCCTCTCCTTCGGCTCCACCCCCGGCTCCGTCATGGCCACGCTCTGCTTCTTCCGCTTCTGGCTCGGGTTTGGGATCGGCGGCGACTACCCGCTCTCCGCCACCATCATGTCCGAGTACGCCAACAAGAAGACGAGGGGCGCCTTCATCGCCGCCGTATTCGCCATGCAGGGCTTCGGCATCCTCACCGGCGGCGTCGTCACGCTCATCGTGTCCGCCGCGTTCCGCGCCGCCTTCCACGCGCCCGCCTACGAGAAGGGCGCCGTCGCATCCACGCCCCCGCAGGCCGACTTCGTGTGGCGCTTCATCCTCATGTTCGGCGCCGTCCCGGCCCTGCTCACCTACTACTGGCGGATGAAGATGCCCGAGACGGCGCGCTACACGGCGCTCGTCGCCAAGAACGCCAAGCAGGCCGCGGCCGACATGTCCAAGGTGCTCCAGGTGGAGATCGCCGCCGAGGACGAAACCAAGGACAACGACGGGGCCGGGGCCGGCGAAGACCGCAACTCGTTCGGGCTCTTCTCCGGCGAGTTCCTTCGGCGGCACGGGCTCCACCTCCTCGGCACGGCCACCTGCTGGTTCCTCCTCGACATCGCCTTCTACTCGCAGAACCTGTTCCAGAAGGACATCTTCACGGCGATCAACTGGATCCCCAAGGCCAAGACGATGAGCGCCCTCGAAGAAGTGCACCGCATCGCGCGCGCGCAGACGCTCATCGCGCTCTGCGGCACGGTGCCGGGCTACTGGTTCACCGTGGCCCTCATCGACCGGATCGGGCGCTTCTGGATCCAGCTCGGCGGATTCTTCTTCATGGCGGTGTTCATGCTGGGGCTGGCCTTCCCGTACCACCACTGGACGACCCCGGGCAACCACATCGGGTTCGTGGTGCTGTACGCGCTCACCTTCTTCTTCGCCAACTTCGGGCCAAACTCCACCACATTCATCGTGCCGGCAGAGATCTTCCCGGCCAGGCTCCGGTCGACGTGCCACGGCATCTCCGCCGCCGCCGGGAAGCTGGGCGCCATCGTGGGGTCGTTCGGGTTCCTGTACCTGGCGCAGAACCAGGACCCCAGCAAGGTGGACCACGGGTACAAGGCCGGCATCGGGGTCAAGAACTCGCTATTCATCCTCGCCGCCTGCAACTTCCTCGGCATGGCCTTCACCTTCTGCGCGCCCGAGTCCAACGGCATCTCGCTCGAGGAGCTCTCCGGCGAGAACGACGACGAGGCGCCGGCGCCGGCGACGCACGCCAGGACGGTGCCCGTGTGA
Protein Sequence
  • >HORVU.MOREX.r3.5HG0523050.1.CDS1
    MAREQLEVLSALDTAKTQWYHFTAIVIAGMGFFTDAYDLFCISLVTKLLGRIYYYREGADAPGSLPPNVAAAVNGVAFCGTLSGQLFFGWLGDRMGRKRVYGMTLMCMVLCSIASGLSFGSTPGSVMATLCFFRFWLGFGIGGDYPLSATIMSEYANKKTRGAFIAAVFAMQGFGILTGGVVTLIVSAAFRAAFHAPAYEKGAVASTPPQADFVWRFILMFGAVPALLTYYWRMKMPETARYTALVAKNAKQAAADMSKVLQVEIAAEDETKDNDGAGAGEDRNSFGLFSGEFLRRHGLHLLGTATCWFLLDIAFYSQNLFQKDIFTAINWIPKAKTMSALEEVHRIARAQTLIALCGTVPGYWFTVALIDRIGRFWIQLGGFFFMAVFMLGLAFPYHHWTTPGNHIGFVVLYALTFFFANFGPNSTTFIVPAEIFPARLRSTCHGISAAAGKLGAIVGSFGFLYLAQNQDPSKVDHGYKAGIGVKNSLFILAACNFLGMAFTFCAPESNGISLEELSGENDDEAPAPATHARTVPV