Information report for HvKCS1
Gene Details
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Functional Descriptions
- The β-Ketoacyl-CoA Synthase HvKCS1, Encoded by Cer-zh, Plays a Key Role in Synthesis of Barley Leaf Wax and Germination of Barley Powdery Mildew.
- Positional cloning of the gene mutated in cer-zh identified an elongase component, β-ketoacyl-CoA synthase (CER-ZH/HvKCS1) that is one of 34 homologous KCSs encoded by the barley genome.
- The biochemical function of HvKCS1 was deduced from wax and cutin analysis of the cer-zh.54 mutant and its heterologous expression in yeast cells. The contribution of HvKCS1 to water barrier properties of the cuticle, and its role in spore germination of barley powdery mildew fungus were also assessed.
- These experiments revealed that CER-ZH/HvKCS1 has a substrate specificity for C16–C20, especially unsaturated, acyl chains, thus playing a major role in total acyl chain elongation for wax biosynthesis.
Functional Keywords
- leaf , cuticle , cutin , wax-biosynthesis , powdery-mildew , mildew , wax
Literature and News
Sequences
cDNA Sequence
- >HORVU.MOREX.r3.4HG0392320.1
GCCACAGGTTTGTTCCGGGAGCCACCGTCGCGGTCTGGCTGTACGCGCACCCTATATATATCGAACCGCCCGCCGAGGCCAACTCACGCTTCCGGTTCCGGCGCCTCGGTCCAACTCACACCAAAGGCGACACTCGACGCGAGTACGTCTACGTCTACGTACGACTCGAGATCCATGGAGTCTGCGCCGGCGGCGGCGTTCATGGAGCGGGAGCGCCTCACGGCGGAGGTGACCTTCTCCGGGGACGCGCAGGCTGCGGCGTCCACTGAGGGGCTGCCCAGCATCATCATCAAGATCCGGCGTCGCCTCCCGGACTTCGCACGCTCCGTCAACCTCAAGTACGTCAAGCTCGGGATCCGGCACGGCGGCAGCCTCACGTCCTACCTGCCCATGCTCTGCGTGCCGGTGCTCGCCGCGGCGGCATACTCCTTCGTCCGCCTCGACGTCATCTACCTCTCCATCGACCTGCTCAGCTGCGTCGCCTGGCTCGGCACCGCCATGCTGCTCCTCACCGTCTACTACATGAAGCGGCCTCGCCCGGTGTACCTCGTCGAGTTCGCGTGCTACAAGCCGGAGGACCAGAACAAGATCTCCAAGGAGGCCTTCCTCGACATGACCGAGAGCACCGGCTGCTTCAACGACGAGACGCTCGCGTTCCAGACCAAGATCACCACCCGCTCCGCTCTCGGCGACGAGACCTACCTGCCCTCGGGCGTCCAGGCGCGCCCGCCGAGGCTCAACATGGCCGAGGCGCGGCTGGAGGCCGAGGCTGTCATGTTCGGCTGCCTTGACGCTCTGTTCGCGTCCACCGGGATCGACCCGCGCCGCGACGTGCGCATCCTCATCGTCAACTGCAGCCTGTTCAACCCGACGCCGTCGCTGGCGTCCATGATCATCCACCATTACAAGATGCGGGAGGACGTCAAGTCGTTCAACCTGGGCGGCATGGGGTGCAGCGCCGGCCTCATCGCCATCGACCTCGCCAAGGACATGCTCCAGGCCAACCCCAACTCGTACGCGGTCGTGCTCAGCACCGAGAACATCACCCTCAACTGGTACTTTGGGAACGACCGCTCCATGCTGCTCTCCAACTGCATCTTCCGCATGGGCGGCGCCGCGGCCCTGCTCTCGAACCGGCGCGCCGACGCCGGGCGCGCCAAGTACCGCCTCCTCCACACGGTGCGGACGCACAAGGGCGCCACGGACGAGTGCTTCAACTGCGTGTACCAGCGCGAGGACGATGTGGGCAAGGTGGGCGTGTCGCTGGCGCGGGAGCTCATGACGGTGGCCGGCGACGCGCTCAAGACCAACATCACCACGCTGGGGCCACTGGTGCTGCCCCTCAGCGAGCAGCTCAAGTTCCTCAAGTCGCTCATGATGCGCCGCGTGTTCCGCGCCAAGGGGGTGCGCCCCTACATCCCCAACTTCCGGCGCGCGTTCGAGCACTTCTGCGTGCACGCCGGCGGGCGCGCCGTGCTGGAGGAGGTGCAGCGCAGCCTGAGCCTGGAGGACAAGGACATGGAGCCCAGCAGGTGCGCCCTGCACCGCTTCGGCAACACCAGCAGCAGCTCGCTCTGGTACGAGCTCGCCTATGCCGAGGCCAAGGGCCGGGTGAAGCGCGGCAACCGCGTCTGGCAGATCGGCTTCGGGTCGGGCTTCAAGTGCAACAGCGCCGTGTGGCGCGCGCTCCGCGACGTGCCGCCCGTGTCGCCCCCGCACGCCGGCGCCGCTGCGCCTGGACCGGAGAAGAAGGCGGCCTGCAACCCGTGGGTGGACTGCGTCGCCAAGTACCCGCCCAAGGCGTACGTCTGATGATCATTACGCCGCCGCCGCTGCTGCTGCATAACTCCACACGAAGATTACATGATTCTTTTCTCCCCACCGCAGTGTAAAACATCACAAGTGCGGTGGGGAGATTATATACTCCTACTATACTTTTTCTGTATTTCCTTTCCCGTAGGGTGGCTGTAATTCAGGTTCCATGGGCGAGCAGTGCCGTTGTTTTCAGCTTTTCTACTTCAAGTGTGAATTACTATAAACGACAATACTCCTCCTTATATATATACGACGAGGTTTCATTC
CDS Sequence
- >HORVU.MOREX.r3.4HG0392320.1
ATGGAGTCTGCGCCGGCGGCGGCGTTCATGGAGCGGGAGCGCCTCACGGCGGAGGTGACCTTCTCCGGGGACGCGCAGGCTGCGGCGTCCACTGAGGGGCTGCCCAGCATCATCATCAAGATCCGGCGTCGCCTCCCGGACTTCGCACGCTCCGTCAACCTCAAGTACGTCAAGCTCGGGATCCGGCACGGCGGCAGCCTCACGTCCTACCTGCCCATGCTCTGCGTGCCGGTGCTCGCCGCGGCGGCATACTCCTTCGTCCGCCTCGACGTCATCTACCTCTCCATCGACCTGCTCAGCTGCGTCGCCTGGCTCGGCACCGCCATGCTGCTCCTCACCGTCTACTACATGAAGCGGCCTCGCCCGGTGTACCTCGTCGAGTTCGCGTGCTACAAGCCGGAGGACCAGAACAAGATCTCCAAGGAGGCCTTCCTCGACATGACCGAGAGCACCGGCTGCTTCAACGACGAGACGCTCGCGTTCCAGACCAAGATCACCACCCGCTCCGCTCTCGGCGACGAGACCTACCTGCCCTCGGGCGTCCAGGCGCGCCCGCCGAGGCTCAACATGGCCGAGGCGCGGCTGGAGGCCGAGGCTGTCATGTTCGGCTGCCTTGACGCTCTGTTCGCGTCCACCGGGATCGACCCGCGCCGCGACGTGCGCATCCTCATCGTCAACTGCAGCCTGTTCAACCCGACGCCGTCGCTGGCGTCCATGATCATCCACCATTACAAGATGCGGGAGGACGTCAAGTCGTTCAACCTGGGCGGCATGGGGTGCAGCGCCGGCCTCATCGCCATCGACCTCGCCAAGGACATGCTCCAGGCCAACCCCAACTCGTACGCGGTCGTGCTCAGCACCGAGAACATCACCCTCAACTGGTACTTTGGGAACGACCGCTCCATGCTGCTCTCCAACTGCATCTTCCGCATGGGCGGCGCCGCGGCCCTGCTCTCGAACCGGCGCGCCGACGCCGGGCGCGCCAAGTACCGCCTCCTCCACACGGTGCGGACGCACAAGGGCGCCACGGACGAGTGCTTCAACTGCGTGTACCAGCGCGAGGACGATGTGGGCAAGGTGGGCGTGTCGCTGGCGCGGGAGCTCATGACGGTGGCCGGCGACGCGCTCAAGACCAACATCACCACGCTGGGGCCACTGGTGCTGCCCCTCAGCGAGCAGCTCAAGTTCCTCAAGTCGCTCATGATGCGCCGCGTGTTCCGCGCCAAGGGGGTGCGCCCCTACATCCCCAACTTCCGGCGCGCGTTCGAGCACTTCTGCGTGCACGCCGGCGGGCGCGCCGTGCTGGAGGAGGTGCAGCGCAGCCTGAGCCTGGAGGACAAGGACATGGAGCCCAGCAGGTGCGCCCTGCACCGCTTCGGCAACACCAGCAGCAGCTCGCTCTGGTACGAGCTCGCCTATGCCGAGGCCAAGGGCCGGGTGAAGCGCGGCAACCGCGTCTGGCAGATCGGCTTCGGGTCGGGCTTCAAGTGCAACAGCGCCGTGTGGCGCGCGCTCCGCGACGTGCCGCCCGTGTCGCCCCCGCACGCCGGCGCCGCTGCGCCTGGACCGGAGAAGAAGGCGGCCTGCAACCCGTGGGTGGACTGCGTCGCCAAGTACCCGCCCAAGGCGTACGTCTGA
Protein Sequence
- >HORVU.MOREX.r3.4HG0392320.1.CDS1
MESAPAAAFMERERLTAEVTFSGDAQAAASTEGLPSIIIKIRRRLPDFARSVNLKYVKLGIRHGGSLTSYLPMLCVPVLAAAAYSFVRLDVIYLSIDLLSCVAWLGTAMLLLTVYYMKRPRPVYLVEFACYKPEDQNKISKEAFLDMTESTGCFNDETLAFQTKITTRSALGDETYLPSGVQARPPRLNMAEARLEAEAVMFGCLDALFASTGIDPRRDVRILIVNCSLFNPTPSLASMIIHHYKMREDVKSFNLGGMGCSAGLIAIDLAKDMLQANPNSYAVVLSTENITLNWYFGNDRSMLLSNCIFRMGGAAALLSNRRADAGRAKYRLLHTVRTHKGATDECFNCVYQREDDVGKVGVSLARELMTVAGDALKTNITTLGPLVLPLSEQLKFLKSLMMRRVFRAKGVRPYIPNFRRAFEHFCVHAGGRAVLEEVQRSLSLEDKDMEPSRCALHRFGNTSSSSLWYELAYAEAKGRVKRGNRVWQIGFGSGFKCNSAVWRALRDVPPVSPPHAGAAAPGPEKKAACNPWVDCVAKYPPKAYV