Gene Details:

Functional Descriptions:

  • The HvDOF19 transcription factor mediates the abscisic acid-dependent repression of hydrolase genes in germinating barley aleurone.
  • HvDOF19 binds in a specific manner to the pyrimidine box within the GARC of a thiol-protease gene (Al21), and mediates the ABA repression of this gene in the barley aleurone.
  • Silencing of HvDOF19 in transient expression assays diminishes the inhibitory effect of ABA upon expression of the Al21 gene promoter. Transcripts from HvDof17 and HvDOF19 accumulate early in germinating aleurones with a peak at 16 h after seed imbibition (hai), whereas the mRNAs of the GA-induced activator GAMYB remain little expressed.
  • HvDOF17 and HvDOF19 interact with GAMYB in plant cell nuclei, and HvDOF17, but not HvDOF19, interferes with the DNA binding of GAMYB to its target site in the promoter of the Al21 gene. A regulatory model of hydrolase gene expression upon germination is proposed.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >HORVU.MOREX.r3.3HG0306700.1
    ATGCCAAAAGAAGGGTGTGCTGAAGTAAATCATTACGATAAATCTCCATCTCAGAGATCGCAACTAAGCCAATCAACAAGATTTCGACTAGTAATTGAAAATAACGTGAGCAGAACAATTTACAAAAAGGAAAGTGTAGAGACCGAGGATGGCGGAGGTCACATAAAAGTTGTCATGTATGATGGTGGCAATCGAATTGCACCTGACCACTCTCTTGCTTCAGCAAGGGTTGATCTAGTTCTAATTGAAGGATCATTCAATGAACCGAAGCGGGGTTCTTGGTCTAAAGAGGAGTTTGAGGAAAGCATAATAAAACCGCGAAAAGGAATCACAAGGCTAGTGAAAAACGGTACATTTGATCTGACTGATGGGAGTTGTGATCATGAAGGTGCCATTATTATGGATAATTCGCAACAAAGGGAAGTTAAGCTTGGAGTAATGATTGCAGTGCATACAGAAGTAAGAGTTCTTGAAGGAGTATCAAATCCTTTCAAAGTGCAAGAAGGCAAGACAAAAAAGACAGCGTCAAGCAAAAAGCGTAAAAAGCCTTCTCCAGTTCCAACGCGAAACTTGGTACAGACAACCAGCACCCACACAGCACAACATGGCCAAAGTCCCATTACTCCAGACGAGCAACATAATCTTCCACCACCTGTCATTCAAGACGTATTGCAGAAATCAGACCGTGGAAAGCATACCGAATTTCTGACTGATGACACTGCAGAGGATAATCGACTGAATTATCCACTGGCTATTGTGCCACAGATAGTTGAGGGAAATGGCCTAAACGGCAACGGTCAACAATTATCTCAACACATCCCAATGCAAATCTTGTGGCAGCCAGCCAGTGCACACTCAATGCGAAATGGTCAAAACTACAGTCTGCCACAAACAGGCATTCAGAATCCATCGCAGGCAGCAGGATATTGTCCAGCCTATCAACCCTTACTTGCTTGTGAAGATCAGTACACTAGTTTCTCTAATGCAAGTTCTCAACTTGCATTTCCGAACCTTTCTGAATACATGACGTTGAATGATATTCGCCAGTGGACTTCCATCCCTGAAGGGGAGATATCTGTGGAACATGTACATCCTTTAATTGTCCCGTCACAAATTCAAGAGACTTATGGGTCATTTAGATGTGGTCAAGTACTACTTCAATGGGATAAGCCTCATGTGGAGCCCAAGGAACACTGGAACTTTCTTGACGAGCTCATGCCGCTGCATAGCACACAGTCAAGGTTTTATGGAGAAAGTTTCCCCACTACAAGCCTCATGGATAAAGCCCATAAGTTACTGGCCAGTAATTCATCAGGTGATTCTGTTTTGAAGATGATTACCATTATTACGAGTCGAGCCACGCAGGCTCCTCAGAGGAGAGGCATTCTCATTCGTCCCATTGAGCCATATGATTCAGACGCTGACATTTTCAGGCCACCAGTTAAGAAGCAAAGAAATGCCAAATATCAATTGCGGTTTGTCAACAGCGTCTGCAATGACTACTACACCCAGGAACAAATCAAATCAGAGAATGGAAATCTCTTAAAGGTAGCTTTGTATGATGAGAATAATCTGGTGGTCACGTCTGGTCCGCTGTCTTCAGCTTCTGTGGAGGTCGTACTACTTCATGGTGATTTCAATGCTGATGGCCAAGATTATTGGACATCAGAGGAGTTCAGTTCCTGCCTAGTGCATCCACAATCTGTCGAAGAACCACCGGCCTTGGTAGGTGATTGTGCATTGGCACTGACTGATGGAGAGACAGACCTTGGTAATATTAGCTTCCAGATTTCCTCCTTCCATGCCAGAACTGGAAAGTTCAAGATGGGTGTTGAGATTAAAAACGTACGAGAAGCGAGTGCTCAAGAAGGAATCACTAGCCCATTTCTCGTGAGAGTTCGCCAAGGGGAAGAATCAAGTCGCCATCGGATCACATCTCCCGAAGCGTTGCTCAGAGTAAGAATGGAATTGCCCAAACAAGTCTGCGGGCCGCTGCAGTGCGATGCCAGGGAAAACGTCTGCGCGTCACCGGACCAGTCGTCGCCGGAGAGAAATGATTTGGTGGCGTCAAGGGCGGAGGACGATGACAGCATGGTACAGAGTGGGAGCGGCGTGTTATTATTCCCAGCCCCGAGGCCGCCGTCGGAGCAGGTGCAGGTGCCGCAGCCGGAGCCGGGGCTCAACTGCCCGCGCTGCAACTCCACCAACACAAAGTTCTTCAACAACAACTCCCTCACACAGCCCCACCACTTCTGCCGCGAATGCGGCCTTGGGCTGGGCGCCACGGCCACGCTTGGCATGACAGCGGTGCCCGCCGCCGACTGCGACATCGCGGGTCGCGCCGCCGGGGCTCCAGCACCAGCCGCCTACGTAGCTGCCGCGGCAGCGATGTCGACGTCAACGAAGCCTAGCACCACCGCTTGCGGCACCTCTGCGTTAATGTCAATGTCGATGGTGGAGCGGGCCCGGTTGGCCAAGGTGCCGCAGCCGGAGCCGGGGCTCAAGTGCCCGCGCTGCGACTCCACCAACACCAAGTTCTGCTACTTCAACAACTACTCCCTCACCCAGCCCCGTCACTTCTGCCACACATGCGGCCGCTCTTGGACTCGTGGCGGCGCGCTCCGCAGTGTCCGCGTGGGCAGCAGGTACAGCTGCCACGCCAAGCGCAGCACCAAGTCCAAGGCATCCGCTAGAGAACCCGCGGCGGCAGCAGGGACAGCATCGTCTGCATTGACGGCAACGACGCCCAACACTACTTCTTGCACCGGCACCGCACCGCCGGGTTTCAACCAGTACTCCATGTTCTGCACCAAGAGCGCGTCGCCGCACAGCAATTGGTTCGCCGACAACTTCGACCCGGCAAGCCCCGGCTTCGGCTCCCCCGCTAGGCTGCTCTTTCCCCAGCAGGTGTTGAAGGGGGTCGACATAGGGCTACAGAGCGGCAGCGGCGGCGATTGCGAGGACGGAGCCAGTTCCACCACCAAGTGACGCCGCCGGCCAAGAGAGATCCACAGCAGGATTACCCAAGCGACAGGGGCATGAACACAGACGTCGTCTATCAACGGTGACGGCTAGAATTTCTATTCCACCACTAAAAGCATCTCCAACAGCCGTATAAACCCAATAAAAAATATAACACGTCATTATAAC
CDS Sequence
  • >HORVU.MOREX.r3.3HG0306700.1
    ATGCCAAAAGAAGGGTGTGCTGAAGTAAATCATTACGATAAATCTCCATCTCAGAGATCGCAACTAAGCCAATCAACAAGATTTCGACTAGTAATTGAAAATAACGTGAGCAGAACAATTTACAAAAAGGAAAGTGTAGAGACCGAGGATGGCGGAGGTCACATAAAAGTTGTCATGTATGATGGTGGCAATCGAATTGCACCTGACCACTCTCTTGCTTCAGCAAGGGTTGATCTAGTTCTAATTGAAGGATCATTCAATGAACCGAAGCGGGGTTCTTGGTCTAAAGAGGAGTTTGAGGAAAGCATAATAAAACCGCGAAAAGGAATCACAAGGCTAGTGAAAAACGGTACATTTGATCTGACTGATGGGAGTTGTGATCATGAAGGTGCCATTATTATGGATAATTCGCAACAAAGGGAAGTTAAGCTTGGAGTAATGATTGCAGTGCATACAGAAGTAAGAGTTCTTGAAGGAGTATCAAATCCTTTCAAAGTGCAAGAAGGCAAGACAAAAAAGACAGCGTCAAGCAAAAAGCGTAAAAAGCCTTCTCCAGTTCCAACGCGAAACTTGGTACAGACAACCAGCACCCACACAGCACAACATGGCCAAAGTCCCATTACTCCAGACGAGCAACATAATCTTCCACCACCTGTCATTCAAGACGTATTGCAGAAATCAGACCGTGGAAAGCATACCGAATTTCTGACTGATGACACTGCAGAGGATAATCGACTGAATTATCCACTGGCTATTGTGCCACAGATAGTTGAGGGAAATGGCCTAAACGGCAACGGTCAACAATTATCTCAACACATCCCAATGCAAATCTTGTGGCAGCCAGCCAGTGCACACTCAATGCGAAATGGTCAAAACTACAGTCTGCCACAAACAGGCATTCAGAATCCATCGCAGGCAGCAGGATATTGTCCAGCCTATCAACCCTTACTTGCTTGTGAAGATCAGTACACTAGTTTCTCTAATGCAAGTTCTCAACTTGCATTTCCGAACCTTTCTGAATACATGACGTTGAATGATATTCGCCAGTGGACTTCCATCCCTGAAGGGGAGATATCTGTGGAACATGTACATCCTTTAATTGTCCCGTCACAAATTCAAGAGACTTATGGGTCATTTAGATGTGGTCAAGTACTACTTCAATGGGATAAGCCTCATGTGGAGCCCAAGGAACACTGGAACTTTCTTGACGAGCTCATGCCGCTGCATAGCACACAGTCAAGGTTTTATGGAGAAAGTTTCCCCACTACAAGCCTCATGGATAAAGCCCATAAGTTACTGGCCAGTAATTCATCAGGTGATTCTGTTTTGAAGATGATTACCATTATTACGAGTCGAGCCACGCAGGCTCCTCAGAGGAGAGGCATTCTCATTCGTCCCATTGAGCCATATGATTCAGACGCTGACATTTTCAGGCCACCAGTTAAGAAGCAAAGAAATGCCAAATATCAATTGCGGTTTGTCAACAGCGTCTGCAATGACTACTACACCCAGGAACAAATCAAATCAGAGAATGGAAATCTCTTAAAGGTAGCTTTGTATGATGAGAATAATCTGGTGGTCACGTCTGGTCCGCTGTCTTCAGCTTCTGTGGAGGTCGTACTACTTCATGGTGATTTCAATGCTGATGGCCAAGATTATTGGACATCAGAGGAGTTCAGTTCCTGCCTAGTGCATCCACAATCTGTCGAAGAACCACCGGCCTTGGTAGGTGATTGTGCATTGGCACTGACTGATGGAGAGACAGACCTTGGTAATATTAGCTTCCAGATTTCCTCCTTCCATGCCAGAACTGGAAAGTTCAAGATGGGTGTTGAGATTAAAAACGTACGAGAAGCGAGTGCTCAAGAAGGAATCACTAGCCCATTTCTCGTGAGAGTTCGCCAAGGGGAAGAATCAAGTCGCCATCGGATCACATCTCCCGAAGCGTTGCTCAGAGTAAGAATGGAATTGCCCAAACAAGTCTGCGGGCCGCTGCAGTGCGATGCCAGGGAAAACGTCTGCGCGTCACCGGACCAGTCGTCGCCGGAGAGAAATGATTTGGTGGCGTCAAGGGCGGAGGACGATGACAGCATGGTACAGAGTGGGAGCGGCGTGTTATTATTCCCAGCCCCGAGGCCGCCGTCGGAGCAGGTGCAGGTGCCGCAGCCGGAGCCGGGGCTCAACTGCCCGCGCTGCAACTCCACCAACACAAAGTTCTTCAACAACAACTCCCTCACACAGCCCCACCACTTCTGCCGCGAATGCGGCCTTGGGCTGGGCGCCACGGCCACGCTTGGCATGACAGCGGTGCCCGCCGCCGACTGCGACATCGCGGGTCGCGCCGCCGGGGCTCCAGCACCAGCCGCCTACGTAGCTGCCGCGGCAGCGATGTCGACGTCAACGAAGCCTAGCACCACCGCTTGCGGCACCTCTGCGTTAATGTCAATGTCGATGGTGGAGCGGGCCCGGTTGGCCAAGGTGCCGCAGCCGGAGCCGGGGCTCAAGTGCCCGCGCTGCGACTCCACCAACACCAAGTTCTGCTACTTCAACAACTACTCCCTCACCCAGCCCCGTCACTTCTGCCACACATGCGGCCGCTCTTGGACTCGTGGCGGCGCGCTCCGCAGTGTCCGCGTGGGCAGCAGGTACAGCTGCCACGCCAAGCGCAGCACCAAGTCCAAGGCATCCGCTAGAGAACCCGCGGCGGCAGCAGGGACAGCATCGTCTGCATTGACGGCAACGACGCCCAACACTACTTCTTGCACCGGCACCGCACCGCCGGGTTTCAACCAGTACTCCATGTTCTGCACCAAGAGCGCGTCGCCGCACAGCAATTGGTTCGCCGACAACTTCGACCCGGCAAGCCCCGGCTTCGGCTCCCCCGCTAGGCTGCTCTTTCCCCAGCAGGTGTTGAAGGGGGTCGACATAGGGCTACAGAGCGGCAGCGGCGGCGATTGCGAGGACGGAGCCAGTTCCACCACCAAGTGA
Protein Sequence
  • >HORVU.MOREX.r3.3HG0306700.1
    MPKEGCAEVNHYDKSPSQRSQLSQSTRFRLVIENNVSRTIYKKESVETEDGGGHIKVVMYDGGNRIAPDHSLASARVDLVLIEGSFNEPKRGSWSKEEFEESIIKPRKGITRLVKNGTFDLTDGSCDHEGAIIMDNSQQREVKLGVMIAVHTEVRVLEGVSNPFKVQEGKTKKTASSKKRKKPSPVPTRNLVQTTSTHTAQHGQSPITPDEQHNLPPPVIQDVLQKSDRGKHTEFLTDDTAEDNRLNYPLAIVPQIVEGNGLNGNGQQLSQHIPMQILWQPASAHSMRNGQNYSLPQTGIQNPSQAAGYCPAYQPLLACEDQYTSFSNASSQLAFPNLSEYMTLNDIRQWTSIPEGEISVEHVHPLIVPSQIQETYGSFRCGQVLLQWDKPHVEPKEHWNFLDELMPLHSTQSRFYGESFPTTSLMDKAHKLLASNSSGDSVLKMITIITSRATQAPQRRGILIRPIEPYDSDADIFRPPVKKQRNAKYQLRFVNSVCNDYYTQEQIKSENGNLLKVALYDENNLVVTSGPLSSASVEVVLLHGDFNADGQDYWTSEEFSSCLVHPQSVEEPPALVGDCALALTDGETDLGNISFQISSFHARTGKFKMGVEIKNVREASAQEGITSPFLVRVRQGEESSRHRITSPEALLRVRMELPKQVCGPLQCDARENVCASPDQSSPERNDLVASRAEDDDSMVQSGSGVLLFPAPRPPSEQVQVPQPEPGLNCPRCNSTNTKFFNNNSLTQPHHFCRECGLGLGATATLGMTAVPAADCDIAGRAAGAPAPAAYVAAAAAMSTSTKPSTTACGTSALMSMSMVERARLAKVPQPEPGLKCPRCDSTNTKFCYFNNYSLTQPRHFCHTCGRSWTRGGALRSVRVGSRYSCHAKRSTKSKASAREPAAAAGTASSALTATTPNTTSCTGTAPPGFNQYSMFCTKSASPHSNWFADNFDPASPGFGSPARLLFPQQVLKGVDIGLQSGSGGDCEDGASSTTK