Gene Details:

Functional Descriptions:

  • HvPAP14, which belongs to the C1A family of cysteine proteases, was identified in senescing barley (Hordeum vulgare L.) leaves by affinity enrichment using the mechanism-based probe DCG-04 targeting cysteine proteases and subsequent mass spectrometry.
  • Biochemical analyses and expression of a HvPAP14:RFP fusion construct in barley protoplasts was used to identify the subcellular localization and putative substrates of HvPAP14.
  • Overexpression of HvPAP14 in barley revealed that the protease can cleave LHCB proteins and PSBO as well as the large subunit of Rubisco.
  • HvPAP14 is involved in the normal turnover of chloroplast proteins and may have a function in bulk protein degradation during leaf senescence.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >HORVU.MOREX.r3.3HG0304500.1
    ACCACACCACACCCAGCCATCACACAGCATAAGCTAGCTAGCATACACTCAGACTGCCTAGCTAGCATTGCCAAGGTCAAGGTTCCAATCATCAATGGCGAGAGCGATCGTGCTTGCGGCCACCATGGCCGCGGCCGCGCTGTTCATGCTGCTGGCCGCTGCGCCAGCGCCGGCGAGCGCCATGGACTTCGGCGACCACGACCTGGCGTCGGAGGACTCCCTATGGGCGCTGTACGAGCGCTGGCGGGAGCAGCACACCGTGGCGCGCGACCTGGGCGAGAAGGCCCGACGCTTCAACGTGTTCAGGGAGAACGTCCGCCTCATCCACGAGTTCAACCGCGGTGACGCCCCCTACAAGCTCCGCCTCAACCGCTTCGGCGACATGACCGCCGACGAGTTCCGCCGCGCCTACGCCTCCTCCCGCGTCTCTCACCACCGCATGTTCAGCCTTAAGGAGGGTGGTGGCGGTTTCATGCACGGCAGCGCTGCCTCCGTCCGGGACGTGCCGCCGTCGGTGGACTGGCGGCAGAAGGGCGCGGTGACCGCCGTCAAGGACCAAGGGCAGTGCGGCAGCTGCTGGGCTTTCTCGACGATCGCGGCCGTGGAGGGCATCAACGCTATCCGCTCTAAGAACCTCACGTCTCTGTCCGAGCAGCAGCTCGTGGACTGCGACACGAAGTCCAACGCCGGCTGCAACGGCGGCCTCATGGACTACGCCTTCCAGTACATCGCCAAGCATGGCGGGGTGGCCGCCGAGGACGCGTACCCCTACAAGGCCAGGCAGGCCTCCTCCTGCAACAAGAAGCCGTCCGCCGTGGTCACCATCGACGGGTATGAGGACGTGCCCGCCAACGACGAAACCGCGCTGAAGAAGGCGGTGGCTGCACAGCCAGTGGCCGTGGCCATCGAGGCTAGCGGGTCGCACTTCCAGTTCTACTCGGAGGGGGTGTTCGCGGGCAAGTGCGGGACGGAGCTGGACCATGGCGTGGCGGCGGTGGGGTACGGCACCACCGTGGACGGCACCAAGTATTGGATCGTGAAAAACTCATGGGGGCCGGAGTGGGGTGAGAAGGGATACATCCGCATGAAGCGCGATGTGAAGGACAAGGAAGGGCTCTGCGGGATCGCCATGGAGGCGTCCTACCCAGTCAAGACCTCAGCCAACCCGAAGCATGCCGGTGCCCATGACGAGCTCTGAATCTCTCATCGATTATCTAACAAATTATTGGCCCGCAACATGCATGATCAGGGCATTATCGCTAGGGAAAAATAAAGAGTCAATCGCTATGTACCTACACATCAGTGCACTACTACTTAATTGTCACACCTGTAATGCGGTTTCGACCCATTGTATGTAAACAGCTTACGTTATCCTGGTTTCCTCATTGTTGTGC
CDS Sequence
  • >HORVU.MOREX.r3.3HG0304500.1
    ATGGCGAGAGCGATCGTGCTTGCGGCCACCATGGCCGCGGCCGCGCTGTTCATGCTGCTGGCCGCTGCGCCAGCGCCGGCGAGCGCCATGGACTTCGGCGACCACGACCTGGCGTCGGAGGACTCCCTATGGGCGCTGTACGAGCGCTGGCGGGAGCAGCACACCGTGGCGCGCGACCTGGGCGAGAAGGCCCGACGCTTCAACGTGTTCAGGGAGAACGTCCGCCTCATCCACGAGTTCAACCGCGGTGACGCCCCCTACAAGCTCCGCCTCAACCGCTTCGGCGACATGACCGCCGACGAGTTCCGCCGCGCCTACGCCTCCTCCCGCGTCTCTCACCACCGCATGTTCAGCCTTAAGGAGGGTGGTGGCGGTTTCATGCACGGCAGCGCTGCCTCCGTCCGGGACGTGCCGCCGTCGGTGGACTGGCGGCAGAAGGGCGCGGTGACCGCCGTCAAGGACCAAGGGCAGTGCGGCAGCTGCTGGGCTTTCTCGACGATCGCGGCCGTGGAGGGCATCAACGCTATCCGCTCTAAGAACCTCACGTCTCTGTCCGAGCAGCAGCTCGTGGACTGCGACACGAAGTCCAACGCCGGCTGCAACGGCGGCCTCATGGACTACGCCTTCCAGTACATCGCCAAGCATGGCGGGGTGGCCGCCGAGGACGCGTACCCCTACAAGGCCAGGCAGGCCTCCTCCTGCAACAAGAAGCCGTCCGCCGTGGTCACCATCGACGGGTATGAGGACGTGCCCGCCAACGACGAAACCGCGCTGAAGAAGGCGGTGGCTGCACAGCCAGTGGCCGTGGCCATCGAGGCTAGCGGGTCGCACTTCCAGTTCTACTCGGAGGGGGTGTTCGCGGGCAAGTGCGGGACGGAGCTGGACCATGGCGTGGCGGCGGTGGGGTACGGCACCACCGTGGACGGCACCAAGTATTGGATCGTGAAAAACTCATGGGGGCCGGAGTGGGGTGAGAAGGGATACATCCGCATGAAGCGCGATGTGAAGGACAAGGAAGGGCTCTGCGGGATCGCCATGGAGGCGTCCTACCCAGTCAAGACCTCAGCCAACCCGAAGCATGCCGGTGCCCATGACGAGCTCTGA
Protein Sequence
  • >HORVU.MOREX.r3.3HG0304500.1.CDS1
    MARAIVLAATMAAAALFMLLAAAPAPASAMDFGDHDLASEDSLWALYERWREQHTVARDLGEKARRFNVFRENVRLIHEFNRGDAPYKLRLNRFGDMTADEFRRAYASSRVSHHRMFSLKEGGGGFMHGSAASVRDVPPSVDWRQKGAVTAVKDQGQCGSCWAFSTIAAVEGINAIRSKNLTSLSEQQLVDCDTKSNAGCNGGLMDYAFQYIAKHGGVAAEDAYPYKARQASSCNKKPSAVVTIDGYEDVPANDETALKKAVAAQPVAVAIEASGSHFQFYSEGVFAGKCGTELDHGVAAVGYGTTVDGTKYWIVKNSWGPEWGEKGYIRMKRDVKDKEGLCGIAMEASYPVKTSANPKHAGAHDEL