Gene Details:
- Gene ID: HORVU.MOREX.r3.3HG0304500
- Gene Symbol: HvPAP14
- Gene Name: papain-like cysteine proteinase
- Genome: MorexV3_pseudomolecules_assembly
- Species: Hordeum vulgare
Functional Descriptions:
- HvPAP14, which belongs to the C1A family of cysteine proteases, was identified in senescing barley (Hordeum vulgare L.) leaves by affinity enrichment using the mechanism-based probe DCG-04 targeting cysteine proteases and subsequent mass spectrometry.
- Biochemical analyses and expression of a HvPAP14:RFP fusion construct in barley protoplasts was used to identify the subcellular localization and putative substrates of HvPAP14.
- Overexpression of HvPAP14 in barley revealed that the protease can cleave LHCB proteins and PSBO as well as the large subunit of Rubisco.
- HvPAP14 is involved in the normal turnover of chloroplast proteins and may have a function in bulk protein degradation during leaf senescence.
Function-related keywords:
- leaf , leaf-senescence , chloroplast , senescence , protoplasts , protein
Literature:
- Barley cysteine protease PAP14 plays a role in degradation of chloroplast proteins. DOI: 10.1093/jxb/erz356 ; PMID: 31403664
Related News:
Gene Resources:
Orthologs:
Sequences:
cDNA Sequence
- >HORVU.MOREX.r3.3HG0304500.1
ACCACACCACACCCAGCCATCACACAGCATAAGCTAGCTAGCATACACTCAGACTGCCTAGCTAGCATTGCCAAGGTCAAGGTTCCAATCATCAATGGCGAGAGCGATCGTGCTTGCGGCCACCATGGCCGCGGCCGCGCTGTTCATGCTGCTGGCCGCTGCGCCAGCGCCGGCGAGCGCCATGGACTTCGGCGACCACGACCTGGCGTCGGAGGACTCCCTATGGGCGCTGTACGAGCGCTGGCGGGAGCAGCACACCGTGGCGCGCGACCTGGGCGAGAAGGCCCGACGCTTCAACGTGTTCAGGGAGAACGTCCGCCTCATCCACGAGTTCAACCGCGGTGACGCCCCCTACAAGCTCCGCCTCAACCGCTTCGGCGACATGACCGCCGACGAGTTCCGCCGCGCCTACGCCTCCTCCCGCGTCTCTCACCACCGCATGTTCAGCCTTAAGGAGGGTGGTGGCGGTTTCATGCACGGCAGCGCTGCCTCCGTCCGGGACGTGCCGCCGTCGGTGGACTGGCGGCAGAAGGGCGCGGTGACCGCCGTCAAGGACCAAGGGCAGTGCGGCAGCTGCTGGGCTTTCTCGACGATCGCGGCCGTGGAGGGCATCAACGCTATCCGCTCTAAGAACCTCACGTCTCTGTCCGAGCAGCAGCTCGTGGACTGCGACACGAAGTCCAACGCCGGCTGCAACGGCGGCCTCATGGACTACGCCTTCCAGTACATCGCCAAGCATGGCGGGGTGGCCGCCGAGGACGCGTACCCCTACAAGGCCAGGCAGGCCTCCTCCTGCAACAAGAAGCCGTCCGCCGTGGTCACCATCGACGGGTATGAGGACGTGCCCGCCAACGACGAAACCGCGCTGAAGAAGGCGGTGGCTGCACAGCCAGTGGCCGTGGCCATCGAGGCTAGCGGGTCGCACTTCCAGTTCTACTCGGAGGGGGTGTTCGCGGGCAAGTGCGGGACGGAGCTGGACCATGGCGTGGCGGCGGTGGGGTACGGCACCACCGTGGACGGCACCAAGTATTGGATCGTGAAAAACTCATGGGGGCCGGAGTGGGGTGAGAAGGGATACATCCGCATGAAGCGCGATGTGAAGGACAAGGAAGGGCTCTGCGGGATCGCCATGGAGGCGTCCTACCCAGTCAAGACCTCAGCCAACCCGAAGCATGCCGGTGCCCATGACGAGCTCTGAATCTCTCATCGATTATCTAACAAATTATTGGCCCGCAACATGCATGATCAGGGCATTATCGCTAGGGAAAAATAAAGAGTCAATCGCTATGTACCTACACATCAGTGCACTACTACTTAATTGTCACACCTGTAATGCGGTTTCGACCCATTGTATGTAAACAGCTTACGTTATCCTGGTTTCCTCATTGTTGTGC
CDS Sequence
- >HORVU.MOREX.r3.3HG0304500.1
ATGGCGAGAGCGATCGTGCTTGCGGCCACCATGGCCGCGGCCGCGCTGTTCATGCTGCTGGCCGCTGCGCCAGCGCCGGCGAGCGCCATGGACTTCGGCGACCACGACCTGGCGTCGGAGGACTCCCTATGGGCGCTGTACGAGCGCTGGCGGGAGCAGCACACCGTGGCGCGCGACCTGGGCGAGAAGGCCCGACGCTTCAACGTGTTCAGGGAGAACGTCCGCCTCATCCACGAGTTCAACCGCGGTGACGCCCCCTACAAGCTCCGCCTCAACCGCTTCGGCGACATGACCGCCGACGAGTTCCGCCGCGCCTACGCCTCCTCCCGCGTCTCTCACCACCGCATGTTCAGCCTTAAGGAGGGTGGTGGCGGTTTCATGCACGGCAGCGCTGCCTCCGTCCGGGACGTGCCGCCGTCGGTGGACTGGCGGCAGAAGGGCGCGGTGACCGCCGTCAAGGACCAAGGGCAGTGCGGCAGCTGCTGGGCTTTCTCGACGATCGCGGCCGTGGAGGGCATCAACGCTATCCGCTCTAAGAACCTCACGTCTCTGTCCGAGCAGCAGCTCGTGGACTGCGACACGAAGTCCAACGCCGGCTGCAACGGCGGCCTCATGGACTACGCCTTCCAGTACATCGCCAAGCATGGCGGGGTGGCCGCCGAGGACGCGTACCCCTACAAGGCCAGGCAGGCCTCCTCCTGCAACAAGAAGCCGTCCGCCGTGGTCACCATCGACGGGTATGAGGACGTGCCCGCCAACGACGAAACCGCGCTGAAGAAGGCGGTGGCTGCACAGCCAGTGGCCGTGGCCATCGAGGCTAGCGGGTCGCACTTCCAGTTCTACTCGGAGGGGGTGTTCGCGGGCAAGTGCGGGACGGAGCTGGACCATGGCGTGGCGGCGGTGGGGTACGGCACCACCGTGGACGGCACCAAGTATTGGATCGTGAAAAACTCATGGGGGCCGGAGTGGGGTGAGAAGGGATACATCCGCATGAAGCGCGATGTGAAGGACAAGGAAGGGCTCTGCGGGATCGCCATGGAGGCGTCCTACCCAGTCAAGACCTCAGCCAACCCGAAGCATGCCGGTGCCCATGACGAGCTCTGA
Protein Sequence
- >HORVU.MOREX.r3.3HG0304500.1.CDS1
MARAIVLAATMAAAALFMLLAAAPAPASAMDFGDHDLASEDSLWALYERWREQHTVARDLGEKARRFNVFRENVRLIHEFNRGDAPYKLRLNRFGDMTADEFRRAYASSRVSHHRMFSLKEGGGGFMHGSAASVRDVPPSVDWRQKGAVTAVKDQGQCGSCWAFSTIAAVEGINAIRSKNLTSLSEQQLVDCDTKSNAGCNGGLMDYAFQYIAKHGGVAAEDAYPYKARQASSCNKKPSAVVTIDGYEDVPANDETALKKAVAAQPVAVAIEASGSHFQFYSEGVFAGKCGTELDHGVAAVGYGTTVDGTKYWIVKNSWGPEWGEKGYIRMKRDVKDKEGLCGIAMEASYPVKTSANPKHAGAHDEL