Gene Details:

Functional Descriptions:

  • We identified a novel cereal member of CDFs in barley, termed HvMTP1, that localizes to the vacuolar membrane. Unlike its close relative AtMTP1, which is highly selective for Zn2+, HvMTP1 exhibits selectivity for both Zn2+ and Co2+ as assessed by its ability to suppress yeast mutant phenotypes for both metals.
  • Expression of HvMTP1/AtMTP1 chimeras in yeast revealed a five-residue sequence within the AtMTP1 N-segment of the His-rich intracytoplasmic loop that confines specificity to Zn2+.
  • Improving zinc accumulation in cereal endosperm using HvMTP1, a transition metal transporter.
  • The barley (Hordeum vulgare) vacuolar Zn transporter HvMTP1 was expressed under the control of the endosperm-specific D-hordein promoter.
  • We hypothesized that over-expression of HvMTP1 on the strong endosperm-specific D-hordein promoter might provide a strategy to increase endosperm Zn content, thereby providing a proof of principle approach to cereal grain biofortification.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
CDS Sequence
Protein Sequence