Gene Details:

  • Gene ID: Gh_A01G0621
  • Gene Symbol: GhCPK33
  • Gene Name: calcium-dependent protein kinase
  • Genome: TM1_NBI
  • Species: Gossypium hirsutum

Functional Descriptions:

  • GhCPK33 Negatively Regulates Defense against Verticillium dahliae by Phosphorylating GhOPR3.
  • Identified the calcium-dependent protein kinase GhCPK33 from upland cotton (Gossypium hirsutum) as a negative regulator of resistance to V. dahliae that directly manipulates JA biosynthesis.
  • Knockdown of GhCPK33 by Agrobacterium tumefaciens-mediated virus-induced gene silencing constitutively activated JA biosynthesis and JA-mediated defense responses and enhanced resistance to V. dahliae.
  • GhCPK33 phosphorylates GhOPR3 at threonine-246, leading to decreased stability of GhOPR3, which consequently limits JA biosynthesis. We propose that GhCPK33 is a potential molecular target for improving resistance to Verticillium wilt disease in cotton.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Orthologs:

Sequences:

cDNA Sequence
CDS Sequence
  • >Gh_A01G0621
    ATGGGTACTTGCCTGACGAAAAGCAAAGACTCGAAGCCAACGCATAATGGGTATGGATCAGGACCGACAACTACGGCTGCTGTGCACCAACAAAGGTACCAGGAGCCTGTTAGGCCTGCACCAGTTCAACCCCAATTCCACCACATCCCTGAAAAACCAGGTACTCAAACACCTTGGAAGCCAGTGGCTCCATCGCCAAGCCCCAAGCCTGTTGCCCCAAGGGTTGACACCATTCTTGGTAAACCATTTGAAGATATAAGGATGCACTACACAATTGGTAAAGAATTAGGGAAAGGTCAGTTTGGTGTGACTTATCTTTGTATTGAAAATTCAACTGGAAAGCAATATGCCTGCAAGACCATATCCAAAAAGAAATTAGTCACGAGGAATGATAAGGAGGATATGAGAAGGGAGATACAAATTATGCAGCATTTGAGTGGACAGCCTAATATTGTGGAGTTTAAAGGTGCATATGAGGATAAGCTATCTGTTCACCTTGTAATGGAATTATGTGCTGGTGGTGAATTGTTTGATAGGATTATTGCCAAGGGACATTATAGTGAAAGGGCAGCTGCTTCCATTTGTAGGGCAATTGTGAATGTTGTCCATGCTTGCCATTTTATGGGAGTGATGCATAGGGACCTTAAGCCTGAGAATTTCTTGTTGTCTAGCAAGGGTGAGAATGCTCTTTTGAAGGCCACCGATTTCGGCTTGTCGGTGTTCATTGAAGATGGAAAGGTGTACAAGGATATAGTCGGGAGCGCGTACTATGTTGCTCCTGAAGTATTACTGCGAAAATATGGGAAAGAAATCGATATTTGGAGTGCAGGGGTTATATTATATATCTTACTCAGTGGTGTGCCTCCATTTTGGGCAGAAACGGAGAAGAGGATATTTGATGCTATTTTAGAAGGAGAAATTGACTTCGAAAGTCAACCATGGCCTTCTATTTCAGAGAGTGCCAAGGACCTAGTGCGCCAGATGTTAACACAGGATCCGAAAAAGCGGATTACTTCTACTCAAGTCCTAGAGCATCCATGGATAAGAGAAGGTGGAAGTGCATCAGACAAGCCTATAGATAGTGCAGTCCTTTCTAGAATGAAGCAATTCAGAAGAATGAACAAACTAAAGCAACTTGCCTTAAAGGTCATTGCGGAAAATCTTTCTAGCGAAGAAATCCAGGGTCTGAAGCAAATGTTTGCAAACATCGACACTGACAACAGTGGCACGATCACCTATGAAGAACTTAAGACCGGATTAGCTCGACTTGGATCAAAGCTCACAGAGGCAGAGGTGCAGCAACTAATGGAAGCTGCTGACGTGGATGGAAATGGTAGTATTGATTACATTGAATTCATCACTGCTACAATGCATAGACATAGGCTTGAAAGAGATGAACATCTTTACAAAGCTTTTCAACATTTTGATAAGGACAACAGTGGGTATATCACAAGAGATGAGCTAGAAGCAGCCATGAAAGAATACGGAATGGGTGATGATGACACAATCAAGGAAATAATATCCGAAGTTGACACCGACAACGACGGTAAGATCAACTACGAGGAGTTCCGCGATATGATGAGAAGCGGAACCCAACACACACAACTCTTTTAG
Protein Sequence
  • >Gh_A01G0621
    MGTCLTKSKDSKPTHNGYGSGPTTTAAVHQQRYQEPVRPAPVQPQFHHIPEKPGTQTPWKPVAPSPSPKPVAPRVDTILGKPFEDIRMHYTIGKELGKGQFGVTYLCIENSTGKQYACKTISKKKLVTRNDKEDMRREIQIMQHLSGQPNIVEFKGAYEDKLSVHLVMELCAGGELFDRIIAKGHYSERAAASICRAIVNVVHACHFMGVMHRDLKPENFLLSSKGENALLKATDFGLSVFIEDGKVYKDIVGSAYYVAPEVLLRKYGKEIDIWSAGVILYILLSGVPPFWAETEKRIFDAILEGEIDFESQPWPSISESAKDLVRQMLTQDPKKRITSTQVLEHPWIREGGSASDKPIDSAVLSRMKQFRRMNKLKQLALKVIAENLSSEEIQGLKQMFANIDTDNSGTITYEELKTGLARLGSKLTEAEVQQLMEAADVDGNGSIDYIEFITATMHRHRLERDEHLYKAFQHFDKDNSGYITRDELEAAMKEYGMGDDDTIKEIISEVDTDNDGKINYEEFRDMMRSGTQHTQLF