Gene Details:

  • Gene ID: Glyma.19G203300
  • Gene Symbol: RPS28
  • Gene Name: 40S RIBOSOMAL PROTEIN SMALL SUBUNIT S28
  • Genome: Wm82.a4.v1
  • Species: Glycine max

Functional Descriptions:

  • Our results indicate the potential of RPS28 and EIF1 promoters to facilitate future genetic engineering and breeding to improve the quality and yield of soybean, as well as in a wide variety of other plant species.
  • Two FLOWERING LOCUS T (FT) homologs, FT2a and FT5a, have been identified as encoding components of florigen and controlling flowering time in soybean . The expression levels of these two genes are down-regulated by E1, E2, E3, and E4 under long-day (LD) conditions, suggesting that FT2a and FT5a are the major soybean flowering regulation targets . Subsequent studies have shown that E9 is FT2a, with a Ty1/copia-like retrotransposon SORE-1 inserted in its first intron in its recessive allele.
  • Characterization of two constitutive promoters RPS28 and EIF1 for studying soybean growth, development, and symbiotic nodule development.
  • Determined that RPS28 and EIF1 were highly expressed during soybean growth and development, nodule development, and various biotic and abiotic stresses.
  • Fusion of both RPS28 and EIF1 promoters, with or without their first intron, with the reporter gene β-GLUCURONIDASE (uidA) in transgenic soybean, resulted in high GUS activity in seedlings, seeds, and nodules.
  • The potential of RPS28 and EIF1 promoters to facilitate future genetic engineering and breeding to improve the quality and yield of soybean, as well as in a wide variety of other plant species.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Glyma.19G203300.1
    ATGGAGTCTCAGGTGAAGCACGCACTTGTTGTCAAAGTTATGGGTCGTACTGGATCCAGAGGACAAGTGACCCAGGTTAGAGTGAAGTTTTTGGATGATCAGAACCGTCACATCATGAGGAATGTGAAAGGACCTGTGAGAGAAGGAGACATTCTCACCCTACTCGAGTCTGAAAGGGAAGCAAGAAGATTGCGCTAG
CDS Sequence
  • >Glyma.19G203300.1
    ATGGAGTCTCAGGTGAAGCACGCACTTGTTGTCAAAGTTATGGGTCGTACTGGATCCAGAGGACAAGTGACCCAGGTTAGAGTGAAGTTTTTGGATGATCAGAACCGTCACATCATGAGGAATGTGAAAGGACCTGTGAGAGAAGGAGACATTCTCACCCTACTCGAGTCTGAAAGGGAAGCAAGAAGATTGCGCTAG
Protein Sequence
  • >Glyma.19G203300.1
    MESQVKHALVVKVMGRTGSRGQVTQVRVKFLDDQNRHIMRNVKGPVREGDILTLLESEREARRLR*