Gene Details:

Functional Descriptions:

  • Natural variation of MS2 confers male fertility and drives hybrid breeding in soybean.
  • It was reported in the last century that the soybean male sterile MS2 mutant has the highest record with seed set.
  • The cloning and characterization of the MS2 gene in soybean, which encodes a protein that is specifically expressed in the anther.
  • MS2 functions in the tapetum and microspore by directly regulating genes involved in the biosynthesis of secondary metabolites and the lipid metabolism, which is essential for the formation of microspore cell wall.
  • We demonstrated that the MS2 mutant conducts the best in outcrossing rate and makes it an ideal tool in building a cost-effective hybrid system for soybean.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Glyma.10G281800.2
    ATTGCAAAAATCCCTCCCTCTCTCTCATTCACCCTCTGCAGTTCACCTACCATAGTAACAGCCACATTATACGCTCCAATATTTTTATATATATAAAGATATTGCTATGATTATATTATGATATATAAAGAAGGAAGGAACCGGGTGTGTGCTTTCGATATTGTTTGCATCTTCCTTTCTTCCTAGGCACTGATACAAGGAACTTTGAGTTTGCTGAGATGGGACTTTCGGCATAAGGTGGATATGATTCAAACTTTTGGGTTATGATGGTGCAGTTAACTGTTGCTATCAACTAAGTTTTTTTATGGATATCAGTTTTTGTTTCTTCTGCTACTTCTGGTCTGTTTGTTCTTCAATTTTGCTCTTGCTTTTCTTGCTTTTAGTCAAAGGAAGTAACCATTGCCACTTTGCTTTTGCGAGCTCAAAGAGGAAGTTCCACTATCATCATCCAGGTGAGATGAACATCAGCATGCAACACTTAGTAGAGAGACTAAGACCCCTCGTGGGTTTGAACGGATGGGACTATTGCATCTACTGGAAATTGAGTGAAGACCAAAGGTTTCTTGAGTGGTTGGGATGCTGTTGTGCTGGCACTGAAAGCAATCAAAATGCTGGGGAAGAACATCTTTTCCCTGTCTCTTCTGTGGCTTCATGCAGAGATATCACTTATCCACACCCCAGAACAAAGCCCTGTGATCTTCTTTCACAGCTTTCCACTTGCATACCCATAGATAATTCTGGGATTCATGCACAGACCCTATTAACAAACCAACCCAACTGGGTGAACTATTCCAATGGCATGGATCCTAACATTTTGGAAGAAACAATTGGGACCCAGGTTTTGATTTCGGTGCCGGGTGGACTAGTTGAGCTGTTTGTAACTAAACAAGTGTCTGAAGATCATCAACTAATAGATTTTGTGACAAACCAGTGCATTGAAGCCGTGAACCACTCAATGAGCTTCAACATTGACGTGAGCAACATGCAATCAAACCCACTTATACAAGATGAAAACGAAGGGAACAACAACAGGAACAATAATCACTTATTCCATCCATCAGAACATGTCATCACTGATATGGACCACCGCAATATTGGTCTGTGTAATTCTCAACTGAACTTCATGCAGCAGTTCAACTACAACCAGCACAACAGAATGAAGAGCGATGCTGCTTTCTCTGAAGAATACCAAGCTGGTAATTCCTTCCTTCACGACGAGCAAACCAACCCAGAAGATGATCAGGAGCCTGGGCATGAGCATGACACGTATCAGAAAAGCCTCATGACAACAGATTCACAATACGTGGAGGCAAAAGATCAGAAGCAAGAGGAAGACAAGGACTTGATGAAAAACGTTGTTGGCAGATCAGATTCAATGTCAGATTGCAGTGACCAGAACGAAGAAGAGGAATTAGATGGAAAGTATAGGAGGAGGAATGGAAAAGGGAACCAATCCAAGAACCTTGTGGCTGAAAGAAAGAGAAGGAAGAAACTCAATGATAGGCTATATAACCTTCGTTCTTTGGTTCCTAGGATCTCTAAGCTGGATAGGGCATCCATTCTTGGAGATGCCATTGAGTATGTGAAGGATTTGCAGAAGCAAGTGAAGGAGCTCCAAGATGAGCTTGAGGAGAATGCAGACACTGAAAGCAACTGCATGAATATTGGTGTAGGTGCTGAACTTGGGCCAAATGCTGAACATGATAAGGCCCAAACTGGGTTGCATGTGGGAACATCAGGGAATGGATATGTCTCCAAACAAAAGCAGGAAGGTGCTACTGTCATTGATAAGCAGACCCAGCAGATGGAGCCGCAAGTGGAAGTGGCTCTGATAGATGAGAATGAGTATTTTGTGAAGGTTTTCTGTGAGCACAGGCCTGGTGGGTTTGTGAAATTGATGGAAGCGTTGAACACTATTGGCATGGATGTAGTGCATGCCACGGTAACCAGCCACACGGGACTCGTCTCAAATGTTTTTAAAGTGGAAAAAAAGGATAATGAAACGGTTGAGGCTGAAGATGTAAGAGACTCACTGCTAGAGCTTACGCGGAACCGTTATAGAGGGTGGACTCATGAGATGACAGCAACGCCGGAAAATGGGGTGGGAAGGGATCAACATCAACTTCACAACCACCAGCAGATAGGTGCCTACCCGCACCAGTTTCATAGTTAACCTTTGATCGACCATCATGTGTTCTCTCATTATAAACTTTGTTATATTAAACATTTTTAATAATAATTTAGCATGCGTGATTAATATAGAGCTTTCATTACTGGAATAATTTTATTTTACAATCATACAAATGTATGGCAAAACGTTGACAACCCTTAAAAAAACAATTTTTTAAATCATATATATATATATATTTGAGAG
  • >Glyma.10G281800.1
    ATTGCAAAAATCCCTCCCTCTCTCTCATTCACCCTCTGCAGTTCACCTACCATAGTAACAGCCACATTATACGCTCCAATATTTTTATATATATAAAGATATTGCTATGATTATATTATGATATATAAAGAAGGAAGGAACCGGGTGTGTGCTTTCGATATTGTTTGCATCTTCCTTTCTTCCTAGGCACTGATACAAGGAACTTTGAGTTTGCTGAGATGGGACTTTCGGCATAAGGTGGATATGATTCAAACTTTTGGGTTATGATGGTGCAGTTAACTGTTGCTATCAACTAAGTTTTTTTATGGATATCAGTTTTTGTTTCTTCTGCTACTTCTGGTCTGTTTGTTCTTCAATTTTGCTCTTGCTTTTCTTGCTTTTAGTCAAAGGAAGTAACCATTGCCACTTTGCTTTTGCGAGCTCAAAGAGGAAGTTCCACTATCATCATCCAGGTGAGATGAACATCAGCATGCAACACTTAGTAGAGAGACTAAGACCCCTCGTGGGTTTGAACGGATGGGACTATTGCATCTACTGGAAATTGAGTGAAGACCAAAGGTTTCTTGAGTGGTTGGGATGCTGTTGTGCTGGCACTGAAAGCAATCAAAATGCTGGGGAAGAACATCTTTTCCCTGTCTCTTCTGTGGCTTCATGCAGAGATATCACTTATCCACACCCCAGAACAAAGCCCTGTGATCTTCTTTCACAGCTTTCCACTTGCATACCCATAGATAATTCTGGGATTCATGCACAGACCCTATTAACAAACCAACCCAACTGGGTGAACTATTCCAATGGCATGGATCCTAACATTTTGGAAGAAACAATTGGGACCCAGGTTTTGATTTCGGTGCCGGGTGGACTAGTTGAGCTGTTTGTAACTAAACAAGTGTCTGAAGATCATCAACTAATAGATTTTGTGACAAACCAGTGCATTGAAGCCGTGAACCACTCAATGAGCTTCAACATTGACGTGAGCAACATGCAATCAAACCCACTTATACAAGATGAAAACGAAGGGAACAACAACAGGAACAATAATCACTTATTCCATCCATCAGAACATGTCATCACTGATATGGACCACCGCAATATTGGTCTGTGTAATTCTCAACTGAACTTCATGCAGCAGTTCAACTACAACCAGCACAACAGAATGAAGAGCGATGCTGCTTTCTCTGAAGAATACCAAGCTGGTAATTCCTTCCTTCACGACGAGCAAACCAACCCAGAAGATGATCAGGAGCCTGGGCATGAGCATGACACGTATCAGAAAAGCCTCATGACAACAGATTCACAATACGTGGAGGCAAAAGATCAGAAGCAAGAGGAAGACAAGGACTTGATGAAAAACGTTGTTGGCAGATCAGATTCAATGTCAGATTGCAGTGACCAGAACGAAGAAGAGGAATTAGATGGAAAGTATAGGAGGAGGAATGGAAAAGGGAACCAATCCAAGAACCTTGTGGCTGAAAGAAAGAGAAGGAAGAAACTCAATGATAGGCTATATAACCTTCGTTCTTTGGTTCCTAGGATCTCTAAGCTGGATAGGGCATCCATTCTTGGAGATGCCATTGAGTATGTGAAGGATTTGCAGAAGCAAGTGAAGGAGCTCCAAGATGAGCTTGAGGAGAATGCAGACACTGAAAGCAACTGCATGAATATTGGTGTAGGTGCTGAACTTGGGCCAAATGCTGAACATGATAAGGCCCAAACTGGGTTGCATGTGGGAACATCAGGGAATGGATATGTCTCCAAACAAAAGCAGGAAGGTGCTACTGTCATTGATAAGCAGACCCAGCAGATGGAGCCGCAAGTGGAAGTGGCTCTGATAGATGAGAATGAGTATTTTGTGAAGGTTTTCTGTGAGCACAGGCCTGGTGGGTTTGTGAAATTGATGGAAGCGTTGAACACTATTGGCATGGATGTAGTGCATGCCACGGTAACCAGCCACACGGGACTCGTCTCAAATGTTTTTAAAGTGGAAAAAAAGGATAATGAAACGGTTGAGGCTGAAGATGTAAGAGACTCACTGCTAGAGCTTACGCGGAACCGTTATAGAGGGTGGACTCATGAGATGACAGCAACGCCGGAAAATGGGGTGGGAAGGGATCAACATCAACTTCACAACCACCAGCAGATAGGTGCCTACCCGCACCAGTTTCATAGTTAACCTTTGATCGACCATCATGTGTTCTCTCATTATAAACTTTGTTATATTAAACATTTTTAATAATAATTTAGCATGCGTGATTAATATAGAGCTTTCATTACTGGAATAATTTTATTTTACAATCATACAAATGTATGGCAAAACGTTGACAACCCTTAAAAAAACAATTTTTTAAATCATATATATATATATATTTGAGAG
CDS Sequence
  • >Glyma.10G281800.2
    ATGGATATCAGTTTTTGTTTCTTCTGCTACTTCTGGTCTGTTTGTTCTTCAATTTTGCTCTTGCTTTTCTTGCTTTTAGTCAAAGGAAGTAACCATTGCCACTTTGCTTTTGCGAGCTCAAAGAGGAAGTTCCACTATCATCATCCAGGTGAGATGAACATCAGCATGCAACACTTAGTAGAGAGACTAAGACCCCTCGTGGGTTTGAACGGATGGGACTATTGCATCTACTGGAAATTGAGTGAAGACCAAAGGTTTCTTGAGTGGTTGGGATGCTGTTGTGCTGGCACTGAAAGCAATCAAAATGCTGGGGAAGAACATCTTTTCCCTGTCTCTTCTGTGGCTTCATGCAGAGATATCACTTATCCACACCCCAGAACAAAGCCCTGTGATCTTCTTTCACAGCTTTCCACTTGCATACCCATAGATAATTCTGGGATTCATGCACAGACCCTATTAACAAACCAACCCAACTGGGTGAACTATTCCAATGGCATGGATCCTAACATTTTGGAAGAAACAATTGGGACCCAGGTTTTGATTTCGGTGCCGGGTGGACTAGTTGAGCTGTTTGTAACTAAACAAGTGTCTGAAGATCATCAACTAATAGATTTTGTGACAAACCAGTGCATTGAAGCCGTGAACCACTCAATGAGCTTCAACATTGACGTGAGCAACATGCAATCAAACCCACTTATACAAGATGAAAACGAAGGGAACAACAACAGGAACAATAATCACTTATTCCATCCATCAGAACATGTCATCACTGATATGGACCACCGCAATATTGGTCTGTGTAATTCTCAACTGAACTTCATGCAGCAGTTCAACTACAACCAGCACAACAGAATGAAGAGCGATGCTGCTTTCTCTGAAGAATACCAAGCTGGTAATTCCTTCCTTCACGACGAGCAAACCAACCCAGAAGATGATCAGGAGCCTGGGCATGAGCATGACACGTATCAGAAAAGCCTCATGACAACAGATTCACAATACGTGGAGGCAAAAGATCAGAAGCAAGAGGAAGACAAGGACTTGATGAAAAACGTTGTTGGCAGATCAGATTCAATGTCAGATTGCAGTGACCAGAACGAAGAAGAGGAATTAGATGGAAAGTATAGGAGGAGGAATGGAAAAGGGAACCAATCCAAGAACCTTGTGGCTGAAAGAAAGAGAAGGAAGAAACTCAATGATAGGCTATATAACCTTCGTTCTTTGGTTCCTAGGATCTCTAAGCTGGATAGGGCATCCATTCTTGGAGATGCCATTGAGTATGTGAAGGATTTGCAGAAGCAAGTGAAGGAGCTCCAAGATGAGCTTGAGGAGAATGCAGACACTGAAAGCAACTGCATGAATATTGGTGTAGGTGCTGAACTTGGGCCAAATGCTGAACATGATAAGGCCCAAACTGGGTTGCATGTGGGAACATCAGGGAATGGATATGTCTCCAAACAAAAGCAGGAAGGTGCTACTGTCATTGATAAGCAGACCCAGCAGATGGAGCCGCAAGTGGAAGTGGCTCTGATAGATGAGAATGAGTATTTTGTGAAGGTTTTCTGTGAGCACAGGCCTGGTGGGTTTGTGAAATTGATGGAAGCGTTGAACACTATTGGCATGGATGTAGTGCATGCCACGGTAACCAGCCACACGGGACTCGTCTCAAATGTTTTTAAAGTGGAAAAAAAGGATAATGAAACGGTTGAGGCTGAAGATGTAAGAGACTCACTGCTAGAGCTTACGCGGAACCGTTATAGAGGGTGGACTCATGAGATGACAGCAACGCCGGAAAATGGGGTGGGAAGGGATCAACATCAACTTCACAACCACCAGCAGATAGGTGCCTACCCGCACCAGTTTCATAGTTAA
  • >Glyma.10G281800.1
    ATGGATATCAGTTTTTGTTTCTTCTGCTACTTCTGGTCTGTTTGTTCTTCAATTTTGCTCTTGCTTTTCTTGCTTTTAGTCAAAGGAAGTAACCATTGCCACTTTGCTTTTGCGAGCTCAAAGAGGAAGTTCCACTATCATCATCCAGGTGAGATGAACATCAGCATGCAACACTTAGTAGAGAGACTAAGACCCCTCGTGGGTTTGAACGGATGGGACTATTGCATCTACTGGAAATTGAGTGAAGACCAAAGGTTTCTTGAGTGGTTGGGATGCTGTTGTGCTGGCACTGAAAGCAATCAAAATGCTGGGGAAGAACATCTTTTCCCTGTCTCTTCTGTGGCTTCATGCAGAGATATCACTTATCCACACCCCAGAACAAAGCCCTGTGATCTTCTTTCACAGCTTTCCACTTGCATACCCATAGATAATTCTGGGATTCATGCACAGACCCTATTAACAAACCAACCCAACTGGGTGAACTATTCCAATGGCATGGATCCTAACATTTTGGAAGAAACAATTGGGACCCAGGTTTTGATTTCGGTGCCGGGTGGACTAGTTGAGCTGTTTGTAACTAAACAAGTGTCTGAAGATCATCAACTAATAGATTTTGTGACAAACCAGTGCATTGAAGCCGTGAACCACTCAATGAGCTTCAACATTGACGTGAGCAACATGCAATCAAACCCACTTATACAAGATGAAAACGAAGGGAACAACAACAGGAACAATAATCACTTATTCCATCCATCAGAACATGTCATCACTGATATGGACCACCGCAATATTGGTCTGTGTAATTCTCAACTGAACTTCATGCAGCAGTTCAACTACAACCAGCACAACAGAATGAAGAGCGATGCTGCTTTCTCTGAAGAATACCAAGCTGGTAATTCCTTCCTTCACGACGAGCAAACCAACCCAGAAGATGATCAGGAGCCTGGGCATGAGCATGACACGTATCAGAAAAGCCTCATGACAACAGATTCACAATACGTGGAGGCAAAAGATCAGAAGCAAGAGGAAGACAAGGACTTGATGAAAAACGTTGTTGGCAGATCAGATTCAATGTCAGATTGCAGTGACCAGAACGAAGAAGAGGAATTAGATGGAAAGTATAGGAGGAGGAATGGAAAAGGGAACCAATCCAAGAACCTTGTGGCTGAAAGAAAGAGAAGGAAGAAACTCAATGATAGGCTATATAACCTTCGTTCTTTGGTTCCTAGGATCTCTAAGCTGGATAGGGCATCCATTCTTGGAGATGCCATTGAGTATGTGAAGGATTTGCAGAAGCAAGTGAAGGAGCTCCAAGATGAGCTTGAGGAGAATGCAGACACTGAAAGCAACTGCATGAATATTGGTGTAGGTGCTGAACTTGGGCCAAATGCTGAACATGATAAGGCCCAAACTGGGTTGCATGTGGGAACATCAGGGAATGGATATGTCTCCAAACAAAAGCAGGAAGGTGCTACTGTCATTGATAAGCAGACCCAGCAGATGGAGCCGCAAGTGGAAGTGGCTCTGATAGATGAGAATGAGTATTTTGTGAAGGTTTTCTGTGAGCACAGGCCTGGTGGGTTTGTGAAATTGATGGAAGCGTTGAACACTATTGGCATGGATGTAGTGCATGCCACGGTAACCAGCCACACGGGACTCGTCTCAAATGTTTTTAAAGTGGAAAAAAAGGATAATGAAACGGTTGAGGCTGAAGATGTAAGAGACTCACTGCTAGAGCTTACGCGGAACCGTTATAGAGGGTGGACTCATGAGATGACAGCAACGCCGGAAAATGGGGTGGGAAGGGATCAACATCAACTTCACAACCACCAGCAGATAGGTGCCTACCCGCACCAGTTTCATAGTTAA
Protein Sequence
  • >Glyma.10G281800.2
    MDISFCFFCYFWSVCSSILLLLFLLLVKGSNHCHFAFASSKRKFHYHHPGEMNISMQHLVERLRPLVGLNGWDYCIYWKLSEDQRFLEWLGCCCAGTESNQNAGEEHLFPVSSVASCRDITYPHPRTKPCDLLSQLSTCIPIDNSGIHAQTLLTNQPNWVNYSNGMDPNILEETIGTQVLISVPGGLVELFVTKQVSEDHQLIDFVTNQCIEAVNHSMSFNIDVSNMQSNPLIQDENEGNNNRNNNHLFHPSEHVITDMDHRNIGLCNSQLNFMQQFNYNQHNRMKSDAAFSEEYQAGNSFLHDEQTNPEDDQEPGHEHDTYQKSLMTTDSQYVEAKDQKQEEDKDLMKNVVGRSDSMSDCSDQNEEEELDGKYRRRNGKGNQSKNLVAERKRRKKLNDRLYNLRSLVPRISKLDRASILGDAIEYVKDLQKQVKELQDELEENADTESNCMNIGVGAELGPNAEHDKAQTGLHVGTSGNGYVSKQKQEGATVIDKQTQQMEPQVEVALIDENEYFVKVFCEHRPGGFVKLMEALNTIGMDVVHATVTSHTGLVSNVFKVEKKDNETVEAEDVRDSLLELTRNRYRGWTHEMTATPENGVGRDQHQLHNHQQIGAYPHQFHS*
  • >Glyma.10G281800.1
    MDISFCFFCYFWSVCSSILLLLFLLLVKGSNHCHFAFASSKRKFHYHHPGEMNISMQHLVERLRPLVGLNGWDYCIYWKLSEDQRFLEWLGCCCAGTESNQNAGEEHLFPVSSVASCRDITYPHPRTKPCDLLSQLSTCIPIDNSGIHAQTLLTNQPNWVNYSNGMDPNILEETIGTQVLISVPGGLVELFVTKQVSEDHQLIDFVTNQCIEAVNHSMSFNIDVSNMQSNPLIQDENEGNNNRNNNHLFHPSEHVITDMDHRNIGLCNSQLNFMQQFNYNQHNRMKSDAAFSEEYQAGNSFLHDEQTNPEDDQEPGHEHDTYQKSLMTTDSQYVEAKDQKQEEDKDLMKNVVGRSDSMSDCSDQNEEEELDGKYRRRNGKGNQSKNLVAERKRRKKLNDRLYNLRSLVPRISKLDRASILGDAIEYVKDLQKQVKELQDELEENADTESNCMNIGVGAELGPNAEHDKAQTGLHVGTSGNGYVSKQKQEGATVIDKQTQQMEPQVEVALIDENEYFVKVFCEHRPGGFVKLMEALNTIGMDVVHATVTSHTGLVSNVFKVEKKDNETVEAEDVRDSLLELTRNRYRGWTHEMTATPENGVGRDQHQLHNHQQIGAYPHQFHS*