Gene Details:
- Gene ID: Glyma.10G281800
- Gene Symbol: MS2
- Gene Name: male sterile 2
- Genome: Wm82.a4.v1
- Species: Glycine max
Functional Descriptions:
- Natural variation of MS2 confers male fertility and drives hybrid breeding in soybean.
- It was reported in the last century that the soybean male sterile MS2 mutant has the highest record with seed set.
- The cloning and characterization of the MS2 gene in soybean, which encodes a protein that is specifically expressed in the anther.
- MS2 functions in the tapetum and microspore by directly regulating genes involved in the biosynthesis of secondary metabolites and the lipid metabolism, which is essential for the formation of microspore cell wall.
- We demonstrated that the MS2 mutant conducts the best in outcrossing rate and makes it an ideal tool in building a cost-effective hybrid system for soybean.
Function-related keywords:
- fertility , anther , seed , tapetum , breeding , cell-wall , sterile , microspore , male-fertility , secondary-metabolism , secondary-cell-wall , seed-set , lipid-metabolism
Literature:
- Natural variation of MS2 confers male fertility and drives hybrid breeding in soybean. DOI: 10.1111/pbi.14133 ; PMID: 37475199
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >Glyma.10G281800.2
ATTGCAAAAATCCCTCCCTCTCTCTCATTCACCCTCTGCAGTTCACCTACCATAGTAACAGCCACATTATACGCTCCAATATTTTTATATATATAAAGATATTGCTATGATTATATTATGATATATAAAGAAGGAAGGAACCGGGTGTGTGCTTTCGATATTGTTTGCATCTTCCTTTCTTCCTAGGCACTGATACAAGGAACTTTGAGTTTGCTGAGATGGGACTTTCGGCATAAGGTGGATATGATTCAAACTTTTGGGTTATGATGGTGCAGTTAACTGTTGCTATCAACTAAGTTTTTTTATGGATATCAGTTTTTGTTTCTTCTGCTACTTCTGGTCTGTTTGTTCTTCAATTTTGCTCTTGCTTTTCTTGCTTTTAGTCAAAGGAAGTAACCATTGCCACTTTGCTTTTGCGAGCTCAAAGAGGAAGTTCCACTATCATCATCCAGGTGAGATGAACATCAGCATGCAACACTTAGTAGAGAGACTAAGACCCCTCGTGGGTTTGAACGGATGGGACTATTGCATCTACTGGAAATTGAGTGAAGACCAAAGGTTTCTTGAGTGGTTGGGATGCTGTTGTGCTGGCACTGAAAGCAATCAAAATGCTGGGGAAGAACATCTTTTCCCTGTCTCTTCTGTGGCTTCATGCAGAGATATCACTTATCCACACCCCAGAACAAAGCCCTGTGATCTTCTTTCACAGCTTTCCACTTGCATACCCATAGATAATTCTGGGATTCATGCACAGACCCTATTAACAAACCAACCCAACTGGGTGAACTATTCCAATGGCATGGATCCTAACATTTTGGAAGAAACAATTGGGACCCAGGTTTTGATTTCGGTGCCGGGTGGACTAGTTGAGCTGTTTGTAACTAAACAAGTGTCTGAAGATCATCAACTAATAGATTTTGTGACAAACCAGTGCATTGAAGCCGTGAACCACTCAATGAGCTTCAACATTGACGTGAGCAACATGCAATCAAACCCACTTATACAAGATGAAAACGAAGGGAACAACAACAGGAACAATAATCACTTATTCCATCCATCAGAACATGTCATCACTGATATGGACCACCGCAATATTGGTCTGTGTAATTCTCAACTGAACTTCATGCAGCAGTTCAACTACAACCAGCACAACAGAATGAAGAGCGATGCTGCTTTCTCTGAAGAATACCAAGCTGGTAATTCCTTCCTTCACGACGAGCAAACCAACCCAGAAGATGATCAGGAGCCTGGGCATGAGCATGACACGTATCAGAAAAGCCTCATGACAACAGATTCACAATACGTGGAGGCAAAAGATCAGAAGCAAGAGGAAGACAAGGACTTGATGAAAAACGTTGTTGGCAGATCAGATTCAATGTCAGATTGCAGTGACCAGAACGAAGAAGAGGAATTAGATGGAAAGTATAGGAGGAGGAATGGAAAAGGGAACCAATCCAAGAACCTTGTGGCTGAAAGAAAGAGAAGGAAGAAACTCAATGATAGGCTATATAACCTTCGTTCTTTGGTTCCTAGGATCTCTAAGCTGGATAGGGCATCCATTCTTGGAGATGCCATTGAGTATGTGAAGGATTTGCAGAAGCAAGTGAAGGAGCTCCAAGATGAGCTTGAGGAGAATGCAGACACTGAAAGCAACTGCATGAATATTGGTGTAGGTGCTGAACTTGGGCCAAATGCTGAACATGATAAGGCCCAAACTGGGTTGCATGTGGGAACATCAGGGAATGGATATGTCTCCAAACAAAAGCAGGAAGGTGCTACTGTCATTGATAAGCAGACCCAGCAGATGGAGCCGCAAGTGGAAGTGGCTCTGATAGATGAGAATGAGTATTTTGTGAAGGTTTTCTGTGAGCACAGGCCTGGTGGGTTTGTGAAATTGATGGAAGCGTTGAACACTATTGGCATGGATGTAGTGCATGCCACGGTAACCAGCCACACGGGACTCGTCTCAAATGTTTTTAAAGTGGAAAAAAAGGATAATGAAACGGTTGAGGCTGAAGATGTAAGAGACTCACTGCTAGAGCTTACGCGGAACCGTTATAGAGGGTGGACTCATGAGATGACAGCAACGCCGGAAAATGGGGTGGGAAGGGATCAACATCAACTTCACAACCACCAGCAGATAGGTGCCTACCCGCACCAGTTTCATAGTTAACCTTTGATCGACCATCATGTGTTCTCTCATTATAAACTTTGTTATATTAAACATTTTTAATAATAATTTAGCATGCGTGATTAATATAGAGCTTTCATTACTGGAATAATTTTATTTTACAATCATACAAATGTATGGCAAAACGTTGACAACCCTTAAAAAAACAATTTTTTAAATCATATATATATATATATTTGAGAG - >Glyma.10G281800.1
ATTGCAAAAATCCCTCCCTCTCTCTCATTCACCCTCTGCAGTTCACCTACCATAGTAACAGCCACATTATACGCTCCAATATTTTTATATATATAAAGATATTGCTATGATTATATTATGATATATAAAGAAGGAAGGAACCGGGTGTGTGCTTTCGATATTGTTTGCATCTTCCTTTCTTCCTAGGCACTGATACAAGGAACTTTGAGTTTGCTGAGATGGGACTTTCGGCATAAGGTGGATATGATTCAAACTTTTGGGTTATGATGGTGCAGTTAACTGTTGCTATCAACTAAGTTTTTTTATGGATATCAGTTTTTGTTTCTTCTGCTACTTCTGGTCTGTTTGTTCTTCAATTTTGCTCTTGCTTTTCTTGCTTTTAGTCAAAGGAAGTAACCATTGCCACTTTGCTTTTGCGAGCTCAAAGAGGAAGTTCCACTATCATCATCCAGGTGAGATGAACATCAGCATGCAACACTTAGTAGAGAGACTAAGACCCCTCGTGGGTTTGAACGGATGGGACTATTGCATCTACTGGAAATTGAGTGAAGACCAAAGGTTTCTTGAGTGGTTGGGATGCTGTTGTGCTGGCACTGAAAGCAATCAAAATGCTGGGGAAGAACATCTTTTCCCTGTCTCTTCTGTGGCTTCATGCAGAGATATCACTTATCCACACCCCAGAACAAAGCCCTGTGATCTTCTTTCACAGCTTTCCACTTGCATACCCATAGATAATTCTGGGATTCATGCACAGACCCTATTAACAAACCAACCCAACTGGGTGAACTATTCCAATGGCATGGATCCTAACATTTTGGAAGAAACAATTGGGACCCAGGTTTTGATTTCGGTGCCGGGTGGACTAGTTGAGCTGTTTGTAACTAAACAAGTGTCTGAAGATCATCAACTAATAGATTTTGTGACAAACCAGTGCATTGAAGCCGTGAACCACTCAATGAGCTTCAACATTGACGTGAGCAACATGCAATCAAACCCACTTATACAAGATGAAAACGAAGGGAACAACAACAGGAACAATAATCACTTATTCCATCCATCAGAACATGTCATCACTGATATGGACCACCGCAATATTGGTCTGTGTAATTCTCAACTGAACTTCATGCAGCAGTTCAACTACAACCAGCACAACAGAATGAAGAGCGATGCTGCTTTCTCTGAAGAATACCAAGCTGGTAATTCCTTCCTTCACGACGAGCAAACCAACCCAGAAGATGATCAGGAGCCTGGGCATGAGCATGACACGTATCAGAAAAGCCTCATGACAACAGATTCACAATACGTGGAGGCAAAAGATCAGAAGCAAGAGGAAGACAAGGACTTGATGAAAAACGTTGTTGGCAGATCAGATTCAATGTCAGATTGCAGTGACCAGAACGAAGAAGAGGAATTAGATGGAAAGTATAGGAGGAGGAATGGAAAAGGGAACCAATCCAAGAACCTTGTGGCTGAAAGAAAGAGAAGGAAGAAACTCAATGATAGGCTATATAACCTTCGTTCTTTGGTTCCTAGGATCTCTAAGCTGGATAGGGCATCCATTCTTGGAGATGCCATTGAGTATGTGAAGGATTTGCAGAAGCAAGTGAAGGAGCTCCAAGATGAGCTTGAGGAGAATGCAGACACTGAAAGCAACTGCATGAATATTGGTGTAGGTGCTGAACTTGGGCCAAATGCTGAACATGATAAGGCCCAAACTGGGTTGCATGTGGGAACATCAGGGAATGGATATGTCTCCAAACAAAAGCAGGAAGGTGCTACTGTCATTGATAAGCAGACCCAGCAGATGGAGCCGCAAGTGGAAGTGGCTCTGATAGATGAGAATGAGTATTTTGTGAAGGTTTTCTGTGAGCACAGGCCTGGTGGGTTTGTGAAATTGATGGAAGCGTTGAACACTATTGGCATGGATGTAGTGCATGCCACGGTAACCAGCCACACGGGACTCGTCTCAAATGTTTTTAAAGTGGAAAAAAAGGATAATGAAACGGTTGAGGCTGAAGATGTAAGAGACTCACTGCTAGAGCTTACGCGGAACCGTTATAGAGGGTGGACTCATGAGATGACAGCAACGCCGGAAAATGGGGTGGGAAGGGATCAACATCAACTTCACAACCACCAGCAGATAGGTGCCTACCCGCACCAGTTTCATAGTTAACCTTTGATCGACCATCATGTGTTCTCTCATTATAAACTTTGTTATATTAAACATTTTTAATAATAATTTAGCATGCGTGATTAATATAGAGCTTTCATTACTGGAATAATTTTATTTTACAATCATACAAATGTATGGCAAAACGTTGACAACCCTTAAAAAAACAATTTTTTAAATCATATATATATATATATTTGAGAG
CDS Sequence
- >Glyma.10G281800.2
ATGGATATCAGTTTTTGTTTCTTCTGCTACTTCTGGTCTGTTTGTTCTTCAATTTTGCTCTTGCTTTTCTTGCTTTTAGTCAAAGGAAGTAACCATTGCCACTTTGCTTTTGCGAGCTCAAAGAGGAAGTTCCACTATCATCATCCAGGTGAGATGAACATCAGCATGCAACACTTAGTAGAGAGACTAAGACCCCTCGTGGGTTTGAACGGATGGGACTATTGCATCTACTGGAAATTGAGTGAAGACCAAAGGTTTCTTGAGTGGTTGGGATGCTGTTGTGCTGGCACTGAAAGCAATCAAAATGCTGGGGAAGAACATCTTTTCCCTGTCTCTTCTGTGGCTTCATGCAGAGATATCACTTATCCACACCCCAGAACAAAGCCCTGTGATCTTCTTTCACAGCTTTCCACTTGCATACCCATAGATAATTCTGGGATTCATGCACAGACCCTATTAACAAACCAACCCAACTGGGTGAACTATTCCAATGGCATGGATCCTAACATTTTGGAAGAAACAATTGGGACCCAGGTTTTGATTTCGGTGCCGGGTGGACTAGTTGAGCTGTTTGTAACTAAACAAGTGTCTGAAGATCATCAACTAATAGATTTTGTGACAAACCAGTGCATTGAAGCCGTGAACCACTCAATGAGCTTCAACATTGACGTGAGCAACATGCAATCAAACCCACTTATACAAGATGAAAACGAAGGGAACAACAACAGGAACAATAATCACTTATTCCATCCATCAGAACATGTCATCACTGATATGGACCACCGCAATATTGGTCTGTGTAATTCTCAACTGAACTTCATGCAGCAGTTCAACTACAACCAGCACAACAGAATGAAGAGCGATGCTGCTTTCTCTGAAGAATACCAAGCTGGTAATTCCTTCCTTCACGACGAGCAAACCAACCCAGAAGATGATCAGGAGCCTGGGCATGAGCATGACACGTATCAGAAAAGCCTCATGACAACAGATTCACAATACGTGGAGGCAAAAGATCAGAAGCAAGAGGAAGACAAGGACTTGATGAAAAACGTTGTTGGCAGATCAGATTCAATGTCAGATTGCAGTGACCAGAACGAAGAAGAGGAATTAGATGGAAAGTATAGGAGGAGGAATGGAAAAGGGAACCAATCCAAGAACCTTGTGGCTGAAAGAAAGAGAAGGAAGAAACTCAATGATAGGCTATATAACCTTCGTTCTTTGGTTCCTAGGATCTCTAAGCTGGATAGGGCATCCATTCTTGGAGATGCCATTGAGTATGTGAAGGATTTGCAGAAGCAAGTGAAGGAGCTCCAAGATGAGCTTGAGGAGAATGCAGACACTGAAAGCAACTGCATGAATATTGGTGTAGGTGCTGAACTTGGGCCAAATGCTGAACATGATAAGGCCCAAACTGGGTTGCATGTGGGAACATCAGGGAATGGATATGTCTCCAAACAAAAGCAGGAAGGTGCTACTGTCATTGATAAGCAGACCCAGCAGATGGAGCCGCAAGTGGAAGTGGCTCTGATAGATGAGAATGAGTATTTTGTGAAGGTTTTCTGTGAGCACAGGCCTGGTGGGTTTGTGAAATTGATGGAAGCGTTGAACACTATTGGCATGGATGTAGTGCATGCCACGGTAACCAGCCACACGGGACTCGTCTCAAATGTTTTTAAAGTGGAAAAAAAGGATAATGAAACGGTTGAGGCTGAAGATGTAAGAGACTCACTGCTAGAGCTTACGCGGAACCGTTATAGAGGGTGGACTCATGAGATGACAGCAACGCCGGAAAATGGGGTGGGAAGGGATCAACATCAACTTCACAACCACCAGCAGATAGGTGCCTACCCGCACCAGTTTCATAGTTAA - >Glyma.10G281800.1
ATGGATATCAGTTTTTGTTTCTTCTGCTACTTCTGGTCTGTTTGTTCTTCAATTTTGCTCTTGCTTTTCTTGCTTTTAGTCAAAGGAAGTAACCATTGCCACTTTGCTTTTGCGAGCTCAAAGAGGAAGTTCCACTATCATCATCCAGGTGAGATGAACATCAGCATGCAACACTTAGTAGAGAGACTAAGACCCCTCGTGGGTTTGAACGGATGGGACTATTGCATCTACTGGAAATTGAGTGAAGACCAAAGGTTTCTTGAGTGGTTGGGATGCTGTTGTGCTGGCACTGAAAGCAATCAAAATGCTGGGGAAGAACATCTTTTCCCTGTCTCTTCTGTGGCTTCATGCAGAGATATCACTTATCCACACCCCAGAACAAAGCCCTGTGATCTTCTTTCACAGCTTTCCACTTGCATACCCATAGATAATTCTGGGATTCATGCACAGACCCTATTAACAAACCAACCCAACTGGGTGAACTATTCCAATGGCATGGATCCTAACATTTTGGAAGAAACAATTGGGACCCAGGTTTTGATTTCGGTGCCGGGTGGACTAGTTGAGCTGTTTGTAACTAAACAAGTGTCTGAAGATCATCAACTAATAGATTTTGTGACAAACCAGTGCATTGAAGCCGTGAACCACTCAATGAGCTTCAACATTGACGTGAGCAACATGCAATCAAACCCACTTATACAAGATGAAAACGAAGGGAACAACAACAGGAACAATAATCACTTATTCCATCCATCAGAACATGTCATCACTGATATGGACCACCGCAATATTGGTCTGTGTAATTCTCAACTGAACTTCATGCAGCAGTTCAACTACAACCAGCACAACAGAATGAAGAGCGATGCTGCTTTCTCTGAAGAATACCAAGCTGGTAATTCCTTCCTTCACGACGAGCAAACCAACCCAGAAGATGATCAGGAGCCTGGGCATGAGCATGACACGTATCAGAAAAGCCTCATGACAACAGATTCACAATACGTGGAGGCAAAAGATCAGAAGCAAGAGGAAGACAAGGACTTGATGAAAAACGTTGTTGGCAGATCAGATTCAATGTCAGATTGCAGTGACCAGAACGAAGAAGAGGAATTAGATGGAAAGTATAGGAGGAGGAATGGAAAAGGGAACCAATCCAAGAACCTTGTGGCTGAAAGAAAGAGAAGGAAGAAACTCAATGATAGGCTATATAACCTTCGTTCTTTGGTTCCTAGGATCTCTAAGCTGGATAGGGCATCCATTCTTGGAGATGCCATTGAGTATGTGAAGGATTTGCAGAAGCAAGTGAAGGAGCTCCAAGATGAGCTTGAGGAGAATGCAGACACTGAAAGCAACTGCATGAATATTGGTGTAGGTGCTGAACTTGGGCCAAATGCTGAACATGATAAGGCCCAAACTGGGTTGCATGTGGGAACATCAGGGAATGGATATGTCTCCAAACAAAAGCAGGAAGGTGCTACTGTCATTGATAAGCAGACCCAGCAGATGGAGCCGCAAGTGGAAGTGGCTCTGATAGATGAGAATGAGTATTTTGTGAAGGTTTTCTGTGAGCACAGGCCTGGTGGGTTTGTGAAATTGATGGAAGCGTTGAACACTATTGGCATGGATGTAGTGCATGCCACGGTAACCAGCCACACGGGACTCGTCTCAAATGTTTTTAAAGTGGAAAAAAAGGATAATGAAACGGTTGAGGCTGAAGATGTAAGAGACTCACTGCTAGAGCTTACGCGGAACCGTTATAGAGGGTGGACTCATGAGATGACAGCAACGCCGGAAAATGGGGTGGGAAGGGATCAACATCAACTTCACAACCACCAGCAGATAGGTGCCTACCCGCACCAGTTTCATAGTTAA
Protein Sequence
- >Glyma.10G281800.2
MDISFCFFCYFWSVCSSILLLLFLLLVKGSNHCHFAFASSKRKFHYHHPGEMNISMQHLVERLRPLVGLNGWDYCIYWKLSEDQRFLEWLGCCCAGTESNQNAGEEHLFPVSSVASCRDITYPHPRTKPCDLLSQLSTCIPIDNSGIHAQTLLTNQPNWVNYSNGMDPNILEETIGTQVLISVPGGLVELFVTKQVSEDHQLIDFVTNQCIEAVNHSMSFNIDVSNMQSNPLIQDENEGNNNRNNNHLFHPSEHVITDMDHRNIGLCNSQLNFMQQFNYNQHNRMKSDAAFSEEYQAGNSFLHDEQTNPEDDQEPGHEHDTYQKSLMTTDSQYVEAKDQKQEEDKDLMKNVVGRSDSMSDCSDQNEEEELDGKYRRRNGKGNQSKNLVAERKRRKKLNDRLYNLRSLVPRISKLDRASILGDAIEYVKDLQKQVKELQDELEENADTESNCMNIGVGAELGPNAEHDKAQTGLHVGTSGNGYVSKQKQEGATVIDKQTQQMEPQVEVALIDENEYFVKVFCEHRPGGFVKLMEALNTIGMDVVHATVTSHTGLVSNVFKVEKKDNETVEAEDVRDSLLELTRNRYRGWTHEMTATPENGVGRDQHQLHNHQQIGAYPHQFHS* - >Glyma.10G281800.1
MDISFCFFCYFWSVCSSILLLLFLLLVKGSNHCHFAFASSKRKFHYHHPGEMNISMQHLVERLRPLVGLNGWDYCIYWKLSEDQRFLEWLGCCCAGTESNQNAGEEHLFPVSSVASCRDITYPHPRTKPCDLLSQLSTCIPIDNSGIHAQTLLTNQPNWVNYSNGMDPNILEETIGTQVLISVPGGLVELFVTKQVSEDHQLIDFVTNQCIEAVNHSMSFNIDVSNMQSNPLIQDENEGNNNRNNNHLFHPSEHVITDMDHRNIGLCNSQLNFMQQFNYNQHNRMKSDAAFSEEYQAGNSFLHDEQTNPEDDQEPGHEHDTYQKSLMTTDSQYVEAKDQKQEEDKDLMKNVVGRSDSMSDCSDQNEEEELDGKYRRRNGKGNQSKNLVAERKRRKKLNDRLYNLRSLVPRISKLDRASILGDAIEYVKDLQKQVKELQDELEENADTESNCMNIGVGAELGPNAEHDKAQTGLHVGTSGNGYVSKQKQEGATVIDKQTQQMEPQVEVALIDENEYFVKVFCEHRPGGFVKLMEALNTIGMDVVHATVTSHTGLVSNVFKVEKKDNETVEAEDVRDSLLELTRNRYRGWTHEMTATPENGVGRDQHQLHNHQQIGAYPHQFHS*