Gene Details:

  • Gene ID: Glyma.18G258000
  • Gene Symbol: GmSSAcT1
  • Gene Name: BADH-type soyasaponin acetyltransferase
  • Genome: Wm82.a4.v1
  • Species: Glycine max

Functional Descriptions:

  • Identify and characterize GmSSAcT1, encoding a BADH-type soyasaponin acetyltransferase that catalyzes three or four consecutive acetylations on type-A soyasaponins in vitro and in planta.
  • GmSSAcT1 likely evolved from acyltransferases present in leguminous plants involved in isoflavonoid acylation.
  • Loss-of-function mutants of GmSSAcT1 exhibited impaired seed germination, which attribute to the excessive accumulation of null-acetylated type-A soyasaponins.
  • We conclude that GmSSAcT1 not only functions as a detoxification gene for high accumulation of type-A soyasaponins in soybean seeds but is also a promising target for breeding new soybean varieties with lower bitter soyasaponin content.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Glyma.18G258000.1
    GTTGTGGCTTAGTTAATCCTCAGATGGCAGTGGAAAATATCAAAGTCCACGAAGAGTCCAACGTTGCCCCTCCACCATCATCAACACAATCACTATCCCTCCCTTTCACTTTGTATGACACTTTGTTCCTCAGGTTCCCTTCCATTGACTGGATCTTCTTCTATTCCCTCAATGCTCAACAATCAGAACCTTCTTTCTTCTATGCCAATGTGATTCCAAAGCTCAAAGCCTCTCTCTCTCACACCCTCCTACACTACCCTCCTCTCGCTGGCAACATTCTTTGGCCTTCTGATTCCACAATTCCAATCATCAGTTACACCCCTGGTGATGCCGTTTCCGTCGTTGTTGCTGAGTCCAATGCTGAATTCAACCACTTCATAGATTACTCAGTGCCCCATGAAGCAACAGAGTCTCGTTTTTTAGTACCCCACTTGGAATCCTCGGATTCTCGTGCTTCTGCCCTAGCTCTTCAGATCACTCTTTTCCCCAACAAAGGCTTCAGCATTGGCATCAGCATCCACCATGCTGCTGTTGATGGAAGATCTTCCACCATGTTCATCAAGGCTTGGGCTTCTCTGTGCCAACAAATTATTATGAACTATGAAACAACATCACAAAGTGTGGTGGTGCCATCTTTGGTGCCGGAGTTGGAGCCTTCCTTTGACAGAACACTCATCAAAGACCCGGGAAATTGGAACCGCTTCTTGTTAGCAAAATGGTGCCCTAATATTGCTAATGGAAACAGTGATGGTGATGATAATGGGAAGCGAACCGTGAAGATTCTACCTTCCCCACCAAGACTCAAGGAAGCGTTTTCAGCCACGTCAGTGATTAAACCAACAATTGAGGAAGCAGTTAGAGCCACGTTTGTTCTCACACGAGAGGATTTGGAGAAGATAAAGAAAAGGGTCTTCTCCAAGTGGGACCAAGTGAAAGACCCAGAACCAGAACCAGAATCAGAATCAGAATCAAAATCAACGGTTAATTCTTCTTCAAAGCCACCAACTTTATCATCCTTTGTTCTTGCATGTGCATATTCTGTTGTGTGTATTGCAAAAGCGGTTCACGGGGTTGAAAAGGAGAAGCAAAAGTTTGGTTTCTGGTTCCCGGTGGATTATAGGGCTAGGTTAGAGCCTCCAATCCCTGATACCTATTTTGGAAACTGTGTGTGGAGCCACTTGGTGGATGCAGAACCCTTGGACTTCATAAAGGAAGAAGGGCTCGTCCTTGTTGCAAAAAGCATTAACAGAAAAGTGAAGACCTTACACAAGGAAGAAGTTTTCGGTAAATCGTCTTCTCGGTTCATGGCTTTGGCTAAAGAAGGAGCTGAAATGTTGGGAGTGTCGATGTCTAACAAATTTATGGTTTATGAGACTGATTTTGGTTGGGGGAAGCCTGCTAAGGTGGACATTATAAATTTGGATAGGGCTTCGAATTTGACCATGGGGTTGTTGGACAGCAAGGATGGGGATGGAGGTGTTGAAGTGGGGCTCGTTATGCATCAGAAGGTGATGGATCTCTTTGGTACTATTTTTCATGGAGGACTGAAAGATGAGTGATGGATTCGGAGCTCTTGTTTGTTAATTAATTCACCTGAGATGTGAAACATTGCATCTGAGTTCGGTTTATTTAAGTTATAAATGCTTTTGAAGGCTTCTTTGTCGAAAATATAGAGTTTTTCTGGTAGAAAAGCTTTTAAAAAACTCCAAGAGGAAGGTTGTGAGTTTCTATTTCCTGTTTAAGGTGGAATTGTTCTTTCTTTTGTGACTGTATTTAAAGGTTTTTGTTTACATTAATTTTGTCCTTTTAGTATTGTAATTGTAGAGTCATGTGTAGTATAATAATTATGAGTTGCTCTATTATAATAAATAGAGTACCAACTCACGTAGCTTGTGTACGTGTTAAAATAATATATTCAACCTCCTCTGTTTTGAAGTCTCTTCTTTATTTTTCTAACAGAGTGACAGTAGGCAGGGCTTTAAGTTTTGGGAATTGCAAACTGATTTGAATAATTTTGTACATAGATTAAAATAAAATATATTTTAAAAAAGT
CDS Sequence
  • >Glyma.18G258000.1
    ATGGCAGTGGAAAATATCAAAGTCCACGAAGAGTCCAACGTTGCCCCTCCACCATCATCAACACAATCACTATCCCTCCCTTTCACTTTGTATGACACTTTGTTCCTCAGGTTCCCTTCCATTGACTGGATCTTCTTCTATTCCCTCAATGCTCAACAATCAGAACCTTCTTTCTTCTATGCCAATGTGATTCCAAAGCTCAAAGCCTCTCTCTCTCACACCCTCCTACACTACCCTCCTCTCGCTGGCAACATTCTTTGGCCTTCTGATTCCACAATTCCAATCATCAGTTACACCCCTGGTGATGCCGTTTCCGTCGTTGTTGCTGAGTCCAATGCTGAATTCAACCACTTCATAGATTACTCAGTGCCCCATGAAGCAACAGAGTCTCGTTTTTTAGTACCCCACTTGGAATCCTCGGATTCTCGTGCTTCTGCCCTAGCTCTTCAGATCACTCTTTTCCCCAACAAAGGCTTCAGCATTGGCATCAGCATCCACCATGCTGCTGTTGATGGAAGATCTTCCACCATGTTCATCAAGGCTTGGGCTTCTCTGTGCCAACAAATTATTATGAACTATGAAACAACATCACAAAGTGTGGTGGTGCCATCTTTGGTGCCGGAGTTGGAGCCTTCCTTTGACAGAACACTCATCAAAGACCCGGGAAATTGGAACCGCTTCTTGTTAGCAAAATGGTGCCCTAATATTGCTAATGGAAACAGTGATGGTGATGATAATGGGAAGCGAACCGTGAAGATTCTACCTTCCCCACCAAGACTCAAGGAAGCGTTTTCAGCCACGTCAGTGATTAAACCAACAATTGAGGAAGCAGTTAGAGCCACGTTTGTTCTCACACGAGAGGATTTGGAGAAGATAAAGAAAAGGGTCTTCTCCAAGTGGGACCAAGTGAAAGACCCAGAACCAGAACCAGAATCAGAATCAGAATCAAAATCAACGGTTAATTCTTCTTCAAAGCCACCAACTTTATCATCCTTTGTTCTTGCATGTGCATATTCTGTTGTGTGTATTGCAAAAGCGGTTCACGGGGTTGAAAAGGAGAAGCAAAAGTTTGGTTTCTGGTTCCCGGTGGATTATAGGGCTAGGTTAGAGCCTCCAATCCCTGATACCTATTTTGGAAACTGTGTGTGGAGCCACTTGGTGGATGCAGAACCCTTGGACTTCATAAAGGAAGAAGGGCTCGTCCTTGTTGCAAAAAGCATTAACAGAAAAGTGAAGACCTTACACAAGGAAGAAGTTTTCGGTAAATCGTCTTCTCGGTTCATGGCTTTGGCTAAAGAAGGAGCTGAAATGTTGGGAGTGTCGATGTCTAACAAATTTATGGTTTATGAGACTGATTTTGGTTGGGGGAAGCCTGCTAAGGTGGACATTATAAATTTGGATAGGGCTTCGAATTTGACCATGGGGTTGTTGGACAGCAAGGATGGGGATGGAGGTGTTGAAGTGGGGCTCGTTATGCATCAGAAGGTGATGGATCTCTTTGGTACTATTTTTCATGGAGGACTGAAAGATGAGTGA
Protein Sequence
  • >Glyma.18G258000.1
    MAVENIKVHEESNVAPPPSSTQSLSLPFTLYDTLFLRFPSIDWIFFYSLNAQQSEPSFFYANVIPKLKASLSHTLLHYPPLAGNILWPSDSTIPIISYTPGDAVSVVVAESNAEFNHFIDYSVPHEATESRFLVPHLESSDSRASALALQITLFPNKGFSIGISIHHAAVDGRSSTMFIKAWASLCQQIIMNYETTSQSVVVPSLVPELEPSFDRTLIKDPGNWNRFLLAKWCPNIANGNSDGDDNGKRTVKILPSPPRLKEAFSATSVIKPTIEEAVRATFVLTREDLEKIKKRVFSKWDQVKDPEPEPESESESKSTVNSSSKPPTLSSFVLACAYSVVCIAKAVHGVEKEKQKFGFWFPVDYRARLEPPIPDTYFGNCVWSHLVDAEPLDFIKEEGLVLVAKSINRKVKTLHKEEVFGKSSSRFMALAKEGAEMLGVSMSNKFMVYETDFGWGKPAKVDIINLDRASNLTMGLLDSKDGDGGVEVGLVMHQKVMDLFGTIFHGGLKDE*