Information report for GmRIC1|GmCLE1
Gene Details
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Functional Descriptions
- Here, we report that microRNA172c (miR172c) activates soybean (Glycine max) Rhizobia-Induced CLE1 (GmRIC1) and GmRIC2 by removing the transcriptional repression of these genes by Nodule Number Control 1 (NNC1), leading to the activation of the AON pathway.
- These results suggest that both GmRIC1 and GmRIC2 act downstream of NNC1 and that the miR172c/NNC1 module regulates nodule number in soybean via GmRIC1 and GmRIC2.
- NNC1 knockdown resulted in dramatically higher expression levels of GmRIC1 and GmRIC2 at 5 DAI, suggesting an NNC1 inhibits the expression of both genes during nodulation.
- Given this result, we speculated that GmNINa and NNC1 antagonistically regulate GmRIC1 and GmRIC2 expression by competing for cis-element binding during nodulation.
- The soybean (Glycine max) nodulation-suppressive CLE peptide, GmRIC1, functions interspecifically in common white bean (Phaseolus vulgaris), but not in a supernodulating line mutated in the receptor PvNARK.
- Homeologous over-expression of the nodulation-suppressive CLE peptide-encoding soybean gene, GmRIC1, abolished nodulation in wild-type bean, but had no discernible effect on PvNARK-mutant plants. This demonstrates that soybean GmRIC1 can function interspecifically in bean, acting in a PvNARK-dependent manner.
- The GmRIC1 and GmRIC2 genes initiated expression solely in the roots at approximately 3 days after inoculation (DAI) with Nod factor-producing rhizobia, corresponding to the time point of AON, and the expression was up-regulated by cytokinins.
- The results of this study suggest that GmRIC1 and GmRIC2 are good candidates for root-derived signal Q in AON signal transduction.
Functional Keywords
Literature and News
- A GmNINa-miR172c-NNC1 Regulatory Network Coordinates the Nodulation and Autoregulation of Nodulation Pathways in Soybean. DOI: 10.1016/j.molp.2019.06.002 ; PMID: 31201867
- The soybean (Glycine max) nodulation-suppressive CLE peptide, GmRIC1, functions interspecifically in common white bean (Phaseolus vulgaris), but not in a supernodulating line mutated in the receptor PvNARK. DOI: 10.1016/j.molp.2019.06.002 ; PMID: 25040127
- A GmNINa-miR172c-NNC1 Regulatory Network Coordinates the Nodulation and Autoregulation of Nodulation Pathways in Soybean. DOI: 10.1093/pcp/pcr091 ; PMID: 21757457
- Structure–function analysis of the GmRIC1 signal peptide and CLE domain required for nodulation control in soybean. DOI: 10.1093/jxb/ert008 ; PMID: 23386683
Gene Resources
- NCBI ID: 110599574
- UniProt accessions: A0A097I386
- EMBL: KM485635
- AlphaFoldDB: A0A097I386
- GO: GO:0005576 , GO:0016020 , GO:0030154
- InterPro: IPR039617
- PANTHER: PTHR36016 , PTHR36016:SF1
Sequences
cDNA Sequence
- >Glyma.13G292300.1
CATCCAACCTCAAGTAGACCTTGTTCAATATTGATTCAGACTTTACATTTCACCGCATGGCAAATGCAACAATGGCTACTCGAGTGTCTATACTAATTGCACTAATCATCCTCTCCACCTTCTTCATGACTTTGCAGGCTAGTAATCTCCATGGCCATCCCTTCATTCGTGAAAATAATATTGCAGATAGCCATCACTTTCTTCACAAATATTTAGACGACCTATCAAAGCATATACATGTTCAAGATGCTGATGATGCCCCTCACAAAGATGGAAATACGCATAGGCTCGCACCAGAGGGACCAGATCCTCATCATAATTTTGCAACACCACCAAGAAACTAAAGTAGCTTTGTTTGGAGTAGATTTCGATTGTGCATGCCTCATATAGTTGTGTGAGTTAATTATGAGATCGATGCACGGTTTATTTTTAGTACCTTTTCTTCCCTTATTTAAACTTCGTGGTATTTTCAAATAAACCTACTTGTTGCTTTCGAAACCCAGTGTTCTAGAGCATATATATGTAGGTGAAGTACGCATCATTTTTGTTTGTTATTTGTCGGCAGTAGTAGTTGTATGGTATCTCTGTTTCTCTCTTTTCTACGTTCTTTTTCCTCCTTTTCTGCTGTATTTTTGCCCTTTCTACTGTTAGAGGAAGTACAAGTTCAGTGAGGAGGCTAAGTGTCAGGATTTGTCATGTATGGCAATAATATATATGTCACTTTTCTTACTCATATATGGAATCTGCTTATTTTATTTTTAATAGTAAAGTTTTTAATATGTT
CDS Sequence
- >Glyma.13G292300.1
ATGGCAAATGCAACAATGGCTACTCGAGTGTCTATACTAATTGCACTAATCATCCTCTCCACCTTCTTCATGACTTTGCAGGCTAGTAATCTCCATGGCCATCCCTTCATTCGTGAAAATAATATTGCAGATAGCCATCACTTTCTTCACAAATATTTAGACGACCTATCAAAGCATATACATGTTCAAGATGCTGATGATGCCCCTCACAAAGATGGAAATACGCATAGGCTCGCACCAGAGGGACCAGATCCTCATCATAATTTTGCAACACCACCAAGAAACTAA
Protein Sequence
- >Glyma.13G292300.1
MANATMATRVSILIALIILSTFFMTLQASNLHGHPFIRENNIADSHHFLHKYLDDLSKHIHVQDADDAPHKDGNTHRLAPEGPDPHHNFATPPRN*