Information report for GmPpcK2
Gene Details
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Functional Descriptions
- Although the GmPpcK1–4 transcripts are all expressed throughout nodule development, only the nearly identical GmPpcK2/3 homologs are nodule-enhanced and up-/down-regulated in vivo by photosynthate supply from the shoots.
- In situ RT–PCR investigation of the spatial localization of the GmPpcK1–4 and GmPpc7 transcripts in mature nodules is entirely consistent with this view.
- Complementary histochemical and related RNA gel-blot findings with nodulated, GmPpcK1/3 promoter::GUS-expressing T2 plants provide direct experimental evidence that (i) PpcK gene expression is controlled primarily at the transcriptional level| and (ii) the contrasting expression properties of GmPpcK1/3 are conferred largely by regulatory element(s) within the approximately 1.4-kb 5′-upstream region.
- As a result of our multifaceted analyses of GmPpcK1–4, GmPpc7 and PEPC-phosphorylation in the soybean nodule, it is proposed that the GmPpcK2/3 homologs and GmPpc7 together comprise the key molecular ‘downstream players’ in this regulatory phosphorylation system within the mature nodule’s central zone.
Functional Keywords
Literature and News
Gene Resources
- NCBI ID: 547707
- UniProt accessions: Q8GRZ5
- EMBL: AY144182 , AY144183
- AlphaFoldDB: Q8GRZ5
- GO: GO:0004674 , GO:0005524
- InterPro: IPR000719 , IPR008271 , IPR011009 , IPR017441 , IPR050205
- PANTHER: PTHR24349
- SUPFAM: SSF56112
- PROSITE: PS00107 , PS00108 , PS50011
- Gene3D: 1.10.510.10 , 3.30.200.20
- CDD: cd05117
Sequences
cDNA Sequence
- >Glyma.20G222600.1
ATTGAATAGAATGTGGTCAGCCCTAAAGAGGAATTACGAGGTTAGCGAGGAGATTGGTCGCGGTCGTTTCGGCACCATCTTCCGCTGCTTCCACCCACTCTCCAACCAACCCTACGCCTGCAAACTCATCGACAAATCCCTCCTCCTTGACTCCACCGACCGCCACTGCCTCCAGAACGAACCCAAATTCATGTCCCTCCTCTCTCCTCACCCCAACATCCTCCAAATCTTCCATGTCTTCGAGGACGACCACTATCTCTCCATCGTAATGGACCTCTGCCAGCCCCACACGCTTTTCGACCGCATGCTCCATGCTCCCTTCTCGGAGTCCCAGGCCGCCTCCCTCATCAAGAACCTTCTCGAAGCCGTCGCCCACTGCCACCGCCTCGGCGTCGCCCACCGCGACATTAAGCCCGACAATATTCTCTTCGATTCGGCGGACAATCTTAAGCTCGCCGACTTCGGTTCGGCGGAGTGGTTCGGCGACGGGAGGAGCATGAGCGGCGTGGTGGGGACGCCGTACTACGTGGCGCCGGAGGTTCTGTTGGGGAGGGAGTACGATGAGAAGGTTGATGTGTGGAGCTGTGGGGTGATTTTGTATATAATGTTGGCGGGGATTCCCCCTTTTTACGGAGACTCTGCTGCTGAGATCTTTGAGGCTGTGGTTAGGGCTAACCTCAGGTTCCCTTCTAGAATCTTCCGGACTGTGTCCCCCGCCGCCAAGGATCTCTTGAGAAAAATGATCTCTAGGGACTCTTCTAGAAGGTTCTCCGCAGAACAAGCCTTGAGACACCCTTGGATCTTGAGTGCGGGTGACACGGCTGAACTGACTTGAGAATGTAGCTACATGCTGTAGTAGTAGTGTAGCTAATTTTGCCCCTTTGTCTTTTCTCTCTACTAGCTTAATAAGGTCTACTCGTGGTGGCAGAGGAAGGAACGCTTCTGTAAATTTTCATGGTGCAAGTAGTTTTTTTTCTTTCTAGGAATCTTATATCTGAGACTCTGGGTATGAGTCGAGAGAATCTTGTTTTCCACTCTCGGTGATTCTCTCTTTTGTAGTAGAAAGTGAGAAAGTGGGTGATCTCCCAGACTCCCTCCCCCTAGTACAAAGGAGGAGTTTGATTCTACTATCCTTGTAGTTTTTGTATATGTAAATCATAATAAACAATCATTTTGAATGAATGTAAATGGTGTTTAATCTTCT
CDS Sequence
- >Glyma.20G222600.1
ATGTGGTCAGCCCTAAAGAGGAATTACGAGGTTAGCGAGGAGATTGGTCGCGGTCGTTTCGGCACCATCTTCCGCTGCTTCCACCCACTCTCCAACCAACCCTACGCCTGCAAACTCATCGACAAATCCCTCCTCCTTGACTCCACCGACCGCCACTGCCTCCAGAACGAACCCAAATTCATGTCCCTCCTCTCTCCTCACCCCAACATCCTCCAAATCTTCCATGTCTTCGAGGACGACCACTATCTCTCCATCGTAATGGACCTCTGCCAGCCCCACACGCTTTTCGACCGCATGCTCCATGCTCCCTTCTCGGAGTCCCAGGCCGCCTCCCTCATCAAGAACCTTCTCGAAGCCGTCGCCCACTGCCACCGCCTCGGCGTCGCCCACCGCGACATTAAGCCCGACAATATTCTCTTCGATTCGGCGGACAATCTTAAGCTCGCCGACTTCGGTTCGGCGGAGTGGTTCGGCGACGGGAGGAGCATGAGCGGCGTGGTGGGGACGCCGTACTACGTGGCGCCGGAGGTTCTGTTGGGGAGGGAGTACGATGAGAAGGTTGATGTGTGGAGCTGTGGGGTGATTTTGTATATAATGTTGGCGGGGATTCCCCCTTTTTACGGAGACTCTGCTGCTGAGATCTTTGAGGCTGTGGTTAGGGCTAACCTCAGGTTCCCTTCTAGAATCTTCCGGACTGTGTCCCCCGCCGCCAAGGATCTCTTGAGAAAAATGATCTCTAGGGACTCTTCTAGAAGGTTCTCCGCAGAACAAGCCTTGAGACACCCTTGGATCTTGAGTGCGGGTGACACGGCTGAACTGACTTGA
Protein Sequence
- >Glyma.20G222600.1
MWSALKRNYEVSEEIGRGRFGTIFRCFHPLSNQPYACKLIDKSLLLDSTDRHCLQNEPKFMSLLSPHPNILQIFHVFEDDHYLSIVMDLCQPHTLFDRMLHAPFSESQAASLIKNLLEAVAHCHRLGVAHRDIKPDNILFDSADNLKLADFGSAEWFGDGRSMSGVVGTPYYVAPEVLLGREYDEKVDVWSCGVILYIMLAGIPPFYGDSAAEIFEAVVRANLRFPSRIFRTVSPAAKDLLRKMISRDSSRRFSAEQALRHPWILSAGDTAELT*