Gene Details:
- Gene ID: Glyma.03G251400
- Gene Symbol: GmPpcK1
- Gene Name: phosphoenolpyruvate-carboxylase kinase1
- Genome: Wm82.a4.v1
- Species: Glycine max
Functional Descriptions:
- Although the GmPpcK1–4 transcripts are all expressed throughout nodule development, only the nearly identical GmPpcK2/3 homologs are nodule-enhanced and up-/down-regulated in vivo by photosynthate supply from the shoots.
- In situ RT–PCR investigation of the spatial localization of the GmPpcK1–4 and GmPpc7 transcripts in mature nodules is entirely consistent with this view.
- Complementary histochemical and related RNA gel-blot findings with nodulated, GmPpcK1/3 promoter::GUS-expressing T2 plants provide direct experimental evidence that (i) PpcK gene expression is controlled primarily at the transcriptional level| and (ii) the contrasting expression properties of GmPpcK1/3 are conferred largely by regulatory element(s) within the approximately 1.4-kb 5′-upstream region.
- As a result of our multifaceted analyses of GmPpcK1–4, GmPpc7 and PEPC-phosphorylation in the soybean nodule, it is proposed that the GmPpcK2/3 homologs and GmPpc7 together comprise the key molecular ‘downstream players’ in this regulatory phosphorylation system within the mature nodule’s central zone.
Function-related keywords:
Literature:
- The PEP-carboxylase kinase gene family in Glycine max (GmPpcK1-4): an in-depth molecular analysis with nodulated, non-transgenic and transgenic plants. DOI: 10.1111/j.1365-313X.2006.03006.x ; PMID: 17257170
Related News:
Gene Resources:
Orthologs:
Sequences:
cDNA Sequence
- >Glyma.03G251400.1
GAGGGAGCCTTCCCTCTCACGGTTAATACAGTTAAAGAAATACGTGGTGAATCTTGGTTGTGCCGTGTTACATGCAATGAGCATAATAACTGAATAGAAAACAAAGGGAATATTCCCGCAGTATTCTTGAGTTAAGCTTAAAAAAGTGACTATATAAGCCCTTTGCTCTCTATTTTATATATACACAGAAGAATGTATAGAGAAGCAGCGGTGAAGAAAGAGGAATACCAAGTGTTGGAAGAACTCGGGCGAGGCCGTTTCGGCACCGTGTTCCGCTGCTTCCACCGCACTTCAAACAAATTCTACGCGGCTAAACTCATCGAGAAGCGCAGACTCCTAAACGAGGACAGGCGCTGCATCGAAATGGAGGCCAAGGCCATGAGCTTCCTCTCTCCACACCCAAACATCCTCCAAATCATGGACGCCTTCGAGGACGCCGACTCCTGCTCCATAGTCCTGGAGCTCTGCCAGCCACACACCCTCTTGGACCGCATCGCCGCCCAAGGCCCCTTGACGGAGCCCCACGCCGCCAGCCTCCTCAAACAGCTTCTGGAGGCCGTGGCGCACTGCCACGCCCAGGGACTCGCGCACAGGGACATCAAGCCGGAAAACATCCTGTTCGATGAAGGGAACAAGCTTAAGCTCTCAGACTTCGGGTCCGCCGAGTGGTTGGGTGAGGGGAGTAGCATGAGCGGTGTGGTGGGGACGCCCTATTACGTTGCACCCGAAGTGATTATGGGGAGAGAGTACGATGAGAAGGTTGATGTGTGGAGCAGCGGGGTCATTCTTTACGCCATGTTGGCTGGCTTTCCACCCTTTTATGGAGAGTCTGCTCCCGAAATCTTTGAGTCTGTTTTGAGGGCTAATCTTAGGTTTCCCAGCCTAATCTTTAGCTCTGTTTCTGCACCTGCTAAGGACCTCCTTAGGAAAATGATTTCCAGGGATCCCTCTAACCGCATTTCCGCACATCAAGCCTTGCGACACCCGTGGATTTTGACTGGGGCTCTCACCACTGCAACTATATCCAATTTTCTCACTTAATTTGCTGGAATCAAGCTGTTTATGCTTCGAGTAGTCTTTCCTATTAGGTTCTGTTTATTTTTTCTCAACGGACCCTTTTTACTAACTCATGTATAATAGAGGTTTAAAGAGTTTCAGTTTCGGATTGTATAGCAAATAAGAATCCTGCATTTCCCTCCCTTTTCCGTATCTAATTAAATAAAATATTATAAATATTTATCTGCTTT - >Glyma.03G251400.2
GAGGGAGCCTTCCCTCTCACGGTTAATACAGTTAAAGAAATACGTGGTGAATCTTGGTTGTGCCGTGTTACATGCAATGAGCATAATAACTGAATAGAAAACAAAGGGAATATTCCCGCAGTATTCTTGAGTTAAGCTTAAAAAAGTGACTATATAAGCCCTTTGCTCTCTATTTTATATATACACAGAAGAATGTATAGAGAAGCAGCGGTGAAGAAAGAGGAATACCAAGTGTTGGAAGAACTCGGGCGAGGCCGTTTCGGCACCGTGTTCCGCTGCTTCCACCGCACTTCAAACAAATTCTACGCGGCTAAACTCATCGAGAAGCGCAGACTCCTAAACGAGGACAGGCGCTGCATCGAAATGGAGGCCAAGGCCATGAGCTTCCTCTCTCCACACCCAAACATCCTCCAAATCATGGACGCCTTCGAGGACGCCGACTCCTGCTCCATAGTCCTGGAGCTCTGCCAGCCACACACCCTCTTGGACCGCATCGCCGCCCAAGGCCCCTTGACGGAGCCCCACGCCGCCAGCCTCCTCAAACAGCTTCTGGAGGCCGTGGCGCACTGCCACGCCCAGGGACTCGCGCACAGGGACATCAAGCCGGAAAACATCCTGTTCGATGAAGGGAACAAGCTTAAGCTCTCAGACTTCGGGTCCGCCGAGTGGTTGGGTGAGGGGAGTAGCATGAGCGGTGTGGTGGGGACGCCCTATTACGTTGCACCCGAAGTGATTATGGGGAGAGAGTACGATGAGAAGGTTGATGTGTGGAGCAGCGGGGTCATTCTTTACGCCATGTTGGCTGGCTTTCCACCCTTTTATGGAGAGTCTGCTCCCGAAATCTTTGAGTCTGTTTTGAGGGCTAATCTTAGGTTTCCCAGCCTAATCTTTAGCTCTGTTTCTGCACCTGCTAAGGACCTCCTTAGGAAAATGATTTCCAGGGATCCCTCTAACCGCATTTCCGCACATCAAGCCTTGCGACACCCGTGGATTTTGACTGGGGCTCTCACCACTGCAACTATATCCAATTTTCTCACTTAATTTGCTGGAATCAAGCTGTTTATGCTTCGAGTAGTCTTTCCTATTAGGTTCTGTTTATTTTTTCTCAACGGACCCTTTTTACTAACTCATGTATAATAGAGGTTTAAAGAGTTTCAGTTTCGGATTGTATAGCAAATAAGAATCCTGCATTTCCCTCCCTTTTCCGTATCTAATTAAATAAAATATTATAAATATTTATCTGCTTT
CDS Sequence
- >Glyma.03G251400.1
ATGTATAGAGAAGCAGCGGTGAAGAAAGAGGAATACCAAGTGTTGGAAGAACTCGGGCGAGGCCGTTTCGGCACCGTGTTCCGCTGCTTCCACCGCACTTCAAACAAATTCTACGCGGCTAAACTCATCGAGAAGCGCAGACTCCTAAACGAGGACAGGCGCTGCATCGAAATGGAGGCCAAGGCCATGAGCTTCCTCTCTCCACACCCAAACATCCTCCAAATCATGGACGCCTTCGAGGACGCCGACTCCTGCTCCATAGTCCTGGAGCTCTGCCAGCCACACACCCTCTTGGACCGCATCGCCGCCCAAGGCCCCTTGACGGAGCCCCACGCCGCCAGCCTCCTCAAACAGCTTCTGGAGGCCGTGGCGCACTGCCACGCCCAGGGACTCGCGCACAGGGACATCAAGCCGGAAAACATCCTGTTCGATGAAGGGAACAAGCTTAAGCTCTCAGACTTCGGGTCCGCCGAGTGGTTGGGTGAGGGGAGTAGCATGAGCGGTGTGGTGGGGACGCCCTATTACGTTGCACCCGAAGTGATTATGGGGAGAGAGTACGATGAGAAGGTTGATGTGTGGAGCAGCGGGGTCATTCTTTACGCCATGTTGGCTGGCTTTCCACCCTTTTATGGAGAGTCTGCTCCCGAAATCTTTGAGTCTGTTTTGAGGGCTAATCTTAGGTTTCCCAGCCTAATCTTTAGCTCTGTTTCTGCACCTGCTAAGGACCTCCTTAGGAAAATGATTTCCAGGGATCCCTCTAACCGCATTTCCGCACATCAAGCCTTGCGACACCCGTGGATTTTGACTGGGGCTCTCACCACTGCAACTATATCCAATTTTCTCACTTAA - >Glyma.03G251400.2
ATGTATAGAGAAGCAGCGGTGAAGAAAGAGGAATACCAAGTGTTGGAAGAACTCGGGCGAGGCCGTTTCGGCACCGTGTTCCGCTGCTTCCACCGCACTTCAAACAAATTCTACGCGGCTAAACTCATCGAGAAGCGCAGACTCCTAAACGAGGACAGGCGCTGCATCGAAATGGAGGCCAAGGCCATGAGCTTCCTCTCTCCACACCCAAACATCCTCCAAATCATGGACGCCTTCGAGGACGCCGACTCCTGCTCCATAGTCCTGGAGCTCTGCCAGCCACACACCCTCTTGGACCGCATCGCCGCCCAAGGCCCCTTGACGGAGCCCCACGCCGCCAGCCTCCTCAAACAGCTTCTGGAGGCCGTGGCGCACTGCCACGCCCAGGGACTCGCGCACAGGGACATCAAGCCGGAAAACATCCTGTTCGATGAAGGGAACAAGCTTAAGCTCTCAGACTTCGGGTCCGCCGAGTGGTTGGGTGAGGGGAGTAGCATGAGCGGTGTGGTGGGGACGCCCTATTACGTTGCACCCGAAGTGATTATGGGGAGAGAGTACGATGAGAAGGTTGATGTGTGGAGCAGCGGGGTCATTCTTTACGCCATGTTGGCTGGCTTTCCACCCTTTTATGGAGAGTCTGCTCCCGAAATCTTTGAGTCTGTTTTGAGGGCTAATCTTAGGTTTCCCAGCCTAATCTTTAGCTCTGTTTCTGCACCTGCTAAGGACCTCCTTAGGAAAATGATTTCCAGGGATCCCTCTAACCGCATTTCCGCACATCAAGCCTTGCGACACCCGTGGATTTTGACTGGGGCTCTCACCACTGCAACTATATCCAATTTTCTCACTTAA
Protein Sequence
- >Glyma.03G251400.1
MYREAAVKKEEYQVLEELGRGRFGTVFRCFHRTSNKFYAAKLIEKRRLLNEDRRCIEMEAKAMSFLSPHPNILQIMDAFEDADSCSIVLELCQPHTLLDRIAAQGPLTEPHAASLLKQLLEAVAHCHAQGLAHRDIKPENILFDEGNKLKLSDFGSAEWLGEGSSMSGVVGTPYYVAPEVIMGREYDEKVDVWSSGVILYAMLAGFPPFYGESAPEIFESVLRANLRFPSLIFSSVSAPAKDLLRKMISRDPSNRISAHQALRHPWILTGALTTATISNFLT* - >Glyma.03G251400.2
MYREAAVKKEEYQVLEELGRGRFGTVFRCFHRTSNKFYAAKLIEKRRLLNEDRRCIEMEAKAMSFLSPHPNILQIMDAFEDADSCSIVLELCQPHTLLDRIAAQGPLTEPHAASLLKQLLEAVAHCHAQGLAHRDIKPENILFDEGNKLKLSDFGSAEWLGEGSSMSGVVGTPYYVAPEVIMGREYDEKVDVWSSGVILYAMLAGFPPFYGESAPEIFESVLRANLRFPSLIFSSVSAPAKDLLRKMISRDPSNRISAHQALRHPWILTGALTTATISNFLT*