Gene Details:

  • Gene ID: Glyma.03G251400
  • Gene Symbol: GmPpcK1
  • Gene Name: phosphoenolpyruvate-carboxylase kinase1
  • Genome: Wm82.a4.v1
  • Species: Glycine max

Functional Descriptions:

  • Although the GmPpcK1–4 transcripts are all expressed throughout nodule development, only the nearly identical GmPpcK2/3 homologs are nodule-enhanced and up-/down-regulated in vivo by photosynthate supply from the shoots.
  • In situ RT–PCR investigation of the spatial localization of the GmPpcK1–4 and GmPpc7 transcripts in mature nodules is entirely consistent with this view.
  • Complementary histochemical and related RNA gel-blot findings with nodulated, GmPpcK1/3 promoter::GUS-expressing T2 plants provide direct experimental evidence that (i) PpcK gene expression is controlled primarily at the transcriptional level| and (ii) the contrasting expression properties of GmPpcK1/3 are conferred largely by regulatory element(s) within the approximately 1.4-kb 5′-upstream region.
  • As a result of our multifaceted analyses of GmPpcK1–4, GmPpc7 and PEPC-phosphorylation in the soybean nodule, it is proposed that the GmPpcK2/3 homologs and GmPpc7 together comprise the key molecular ‘downstream players’ in this regulatory phosphorylation system within the mature nodule’s central zone.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Glyma.03G251400.1
    GAGGGAGCCTTCCCTCTCACGGTTAATACAGTTAAAGAAATACGTGGTGAATCTTGGTTGTGCCGTGTTACATGCAATGAGCATAATAACTGAATAGAAAACAAAGGGAATATTCCCGCAGTATTCTTGAGTTAAGCTTAAAAAAGTGACTATATAAGCCCTTTGCTCTCTATTTTATATATACACAGAAGAATGTATAGAGAAGCAGCGGTGAAGAAAGAGGAATACCAAGTGTTGGAAGAACTCGGGCGAGGCCGTTTCGGCACCGTGTTCCGCTGCTTCCACCGCACTTCAAACAAATTCTACGCGGCTAAACTCATCGAGAAGCGCAGACTCCTAAACGAGGACAGGCGCTGCATCGAAATGGAGGCCAAGGCCATGAGCTTCCTCTCTCCACACCCAAACATCCTCCAAATCATGGACGCCTTCGAGGACGCCGACTCCTGCTCCATAGTCCTGGAGCTCTGCCAGCCACACACCCTCTTGGACCGCATCGCCGCCCAAGGCCCCTTGACGGAGCCCCACGCCGCCAGCCTCCTCAAACAGCTTCTGGAGGCCGTGGCGCACTGCCACGCCCAGGGACTCGCGCACAGGGACATCAAGCCGGAAAACATCCTGTTCGATGAAGGGAACAAGCTTAAGCTCTCAGACTTCGGGTCCGCCGAGTGGTTGGGTGAGGGGAGTAGCATGAGCGGTGTGGTGGGGACGCCCTATTACGTTGCACCCGAAGTGATTATGGGGAGAGAGTACGATGAGAAGGTTGATGTGTGGAGCAGCGGGGTCATTCTTTACGCCATGTTGGCTGGCTTTCCACCCTTTTATGGAGAGTCTGCTCCCGAAATCTTTGAGTCTGTTTTGAGGGCTAATCTTAGGTTTCCCAGCCTAATCTTTAGCTCTGTTTCTGCACCTGCTAAGGACCTCCTTAGGAAAATGATTTCCAGGGATCCCTCTAACCGCATTTCCGCACATCAAGCCTTGCGACACCCGTGGATTTTGACTGGGGCTCTCACCACTGCAACTATATCCAATTTTCTCACTTAATTTGCTGGAATCAAGCTGTTTATGCTTCGAGTAGTCTTTCCTATTAGGTTCTGTTTATTTTTTCTCAACGGACCCTTTTTACTAACTCATGTATAATAGAGGTTTAAAGAGTTTCAGTTTCGGATTGTATAGCAAATAAGAATCCTGCATTTCCCTCCCTTTTCCGTATCTAATTAAATAAAATATTATAAATATTTATCTGCTTT
  • >Glyma.03G251400.2
    GAGGGAGCCTTCCCTCTCACGGTTAATACAGTTAAAGAAATACGTGGTGAATCTTGGTTGTGCCGTGTTACATGCAATGAGCATAATAACTGAATAGAAAACAAAGGGAATATTCCCGCAGTATTCTTGAGTTAAGCTTAAAAAAGTGACTATATAAGCCCTTTGCTCTCTATTTTATATATACACAGAAGAATGTATAGAGAAGCAGCGGTGAAGAAAGAGGAATACCAAGTGTTGGAAGAACTCGGGCGAGGCCGTTTCGGCACCGTGTTCCGCTGCTTCCACCGCACTTCAAACAAATTCTACGCGGCTAAACTCATCGAGAAGCGCAGACTCCTAAACGAGGACAGGCGCTGCATCGAAATGGAGGCCAAGGCCATGAGCTTCCTCTCTCCACACCCAAACATCCTCCAAATCATGGACGCCTTCGAGGACGCCGACTCCTGCTCCATAGTCCTGGAGCTCTGCCAGCCACACACCCTCTTGGACCGCATCGCCGCCCAAGGCCCCTTGACGGAGCCCCACGCCGCCAGCCTCCTCAAACAGCTTCTGGAGGCCGTGGCGCACTGCCACGCCCAGGGACTCGCGCACAGGGACATCAAGCCGGAAAACATCCTGTTCGATGAAGGGAACAAGCTTAAGCTCTCAGACTTCGGGTCCGCCGAGTGGTTGGGTGAGGGGAGTAGCATGAGCGGTGTGGTGGGGACGCCCTATTACGTTGCACCCGAAGTGATTATGGGGAGAGAGTACGATGAGAAGGTTGATGTGTGGAGCAGCGGGGTCATTCTTTACGCCATGTTGGCTGGCTTTCCACCCTTTTATGGAGAGTCTGCTCCCGAAATCTTTGAGTCTGTTTTGAGGGCTAATCTTAGGTTTCCCAGCCTAATCTTTAGCTCTGTTTCTGCACCTGCTAAGGACCTCCTTAGGAAAATGATTTCCAGGGATCCCTCTAACCGCATTTCCGCACATCAAGCCTTGCGACACCCGTGGATTTTGACTGGGGCTCTCACCACTGCAACTATATCCAATTTTCTCACTTAATTTGCTGGAATCAAGCTGTTTATGCTTCGAGTAGTCTTTCCTATTAGGTTCTGTTTATTTTTTCTCAACGGACCCTTTTTACTAACTCATGTATAATAGAGGTTTAAAGAGTTTCAGTTTCGGATTGTATAGCAAATAAGAATCCTGCATTTCCCTCCCTTTTCCGTATCTAATTAAATAAAATATTATAAATATTTATCTGCTTT
CDS Sequence
  • >Glyma.03G251400.1
    ATGTATAGAGAAGCAGCGGTGAAGAAAGAGGAATACCAAGTGTTGGAAGAACTCGGGCGAGGCCGTTTCGGCACCGTGTTCCGCTGCTTCCACCGCACTTCAAACAAATTCTACGCGGCTAAACTCATCGAGAAGCGCAGACTCCTAAACGAGGACAGGCGCTGCATCGAAATGGAGGCCAAGGCCATGAGCTTCCTCTCTCCACACCCAAACATCCTCCAAATCATGGACGCCTTCGAGGACGCCGACTCCTGCTCCATAGTCCTGGAGCTCTGCCAGCCACACACCCTCTTGGACCGCATCGCCGCCCAAGGCCCCTTGACGGAGCCCCACGCCGCCAGCCTCCTCAAACAGCTTCTGGAGGCCGTGGCGCACTGCCACGCCCAGGGACTCGCGCACAGGGACATCAAGCCGGAAAACATCCTGTTCGATGAAGGGAACAAGCTTAAGCTCTCAGACTTCGGGTCCGCCGAGTGGTTGGGTGAGGGGAGTAGCATGAGCGGTGTGGTGGGGACGCCCTATTACGTTGCACCCGAAGTGATTATGGGGAGAGAGTACGATGAGAAGGTTGATGTGTGGAGCAGCGGGGTCATTCTTTACGCCATGTTGGCTGGCTTTCCACCCTTTTATGGAGAGTCTGCTCCCGAAATCTTTGAGTCTGTTTTGAGGGCTAATCTTAGGTTTCCCAGCCTAATCTTTAGCTCTGTTTCTGCACCTGCTAAGGACCTCCTTAGGAAAATGATTTCCAGGGATCCCTCTAACCGCATTTCCGCACATCAAGCCTTGCGACACCCGTGGATTTTGACTGGGGCTCTCACCACTGCAACTATATCCAATTTTCTCACTTAA
  • >Glyma.03G251400.2
    ATGTATAGAGAAGCAGCGGTGAAGAAAGAGGAATACCAAGTGTTGGAAGAACTCGGGCGAGGCCGTTTCGGCACCGTGTTCCGCTGCTTCCACCGCACTTCAAACAAATTCTACGCGGCTAAACTCATCGAGAAGCGCAGACTCCTAAACGAGGACAGGCGCTGCATCGAAATGGAGGCCAAGGCCATGAGCTTCCTCTCTCCACACCCAAACATCCTCCAAATCATGGACGCCTTCGAGGACGCCGACTCCTGCTCCATAGTCCTGGAGCTCTGCCAGCCACACACCCTCTTGGACCGCATCGCCGCCCAAGGCCCCTTGACGGAGCCCCACGCCGCCAGCCTCCTCAAACAGCTTCTGGAGGCCGTGGCGCACTGCCACGCCCAGGGACTCGCGCACAGGGACATCAAGCCGGAAAACATCCTGTTCGATGAAGGGAACAAGCTTAAGCTCTCAGACTTCGGGTCCGCCGAGTGGTTGGGTGAGGGGAGTAGCATGAGCGGTGTGGTGGGGACGCCCTATTACGTTGCACCCGAAGTGATTATGGGGAGAGAGTACGATGAGAAGGTTGATGTGTGGAGCAGCGGGGTCATTCTTTACGCCATGTTGGCTGGCTTTCCACCCTTTTATGGAGAGTCTGCTCCCGAAATCTTTGAGTCTGTTTTGAGGGCTAATCTTAGGTTTCCCAGCCTAATCTTTAGCTCTGTTTCTGCACCTGCTAAGGACCTCCTTAGGAAAATGATTTCCAGGGATCCCTCTAACCGCATTTCCGCACATCAAGCCTTGCGACACCCGTGGATTTTGACTGGGGCTCTCACCACTGCAACTATATCCAATTTTCTCACTTAA
Protein Sequence
  • >Glyma.03G251400.1
    MYREAAVKKEEYQVLEELGRGRFGTVFRCFHRTSNKFYAAKLIEKRRLLNEDRRCIEMEAKAMSFLSPHPNILQIMDAFEDADSCSIVLELCQPHTLLDRIAAQGPLTEPHAASLLKQLLEAVAHCHAQGLAHRDIKPENILFDEGNKLKLSDFGSAEWLGEGSSMSGVVGTPYYVAPEVIMGREYDEKVDVWSSGVILYAMLAGFPPFYGESAPEIFESVLRANLRFPSLIFSSVSAPAKDLLRKMISRDPSNRISAHQALRHPWILTGALTTATISNFLT*
  • >Glyma.03G251400.2
    MYREAAVKKEEYQVLEELGRGRFGTVFRCFHRTSNKFYAAKLIEKRRLLNEDRRCIEMEAKAMSFLSPHPNILQIMDAFEDADSCSIVLELCQPHTLLDRIAAQGPLTEPHAASLLKQLLEAVAHCHAQGLAHRDIKPENILFDEGNKLKLSDFGSAEWLGEGSSMSGVVGTPYYVAPEVIMGREYDEKVDVWSSGVILYAMLAGFPPFYGESAPEIFESVLRANLRFPSLIFSSVSAPAKDLLRKMISRDPSNRISAHQALRHPWILTGALTTATISNFLT*