Gene Details:

Functional Descriptions:

  • GmNLP7a inhibits soybean nodulation by interacting with GmNIN1a.
  • Overexpression of GmNLP7a downregulated the expression of GmNIN1a and GmENOD40-1. GmNLP7a interacted with GmNIN1a via the PB1 domain.
  • Downregulation of GmNLP7s increased nodule number, and overexpression of GmNLP7a (GmNLP7aOE) reduced nodule number regardless of nitrate availability, suggesting a negative role for GmNLP7s in nodulation.
  • Our results show that GmNLP7s play a negative role in soybean nodulation via interaction with GmNINa.
  • Overexpression of GmNLP7a reduced nodule number but not nitrogenase activity.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Glyma.09G137000.1
    AATCACCTTTTGTCTCAACAAACAAGTGGCATAATATGCATAGTAAACATTTTATTTTGTTGCATATTCTGATATTCTCCACCTTCTGCAATTTTGCTCAACCAGATTATTTTCTATTGTGTAGTTCTCATTTCTCAACTCCCATCATCATTACAAAGTTCCAAACTTAATCACTCTGTACACACCAATTTTCAAGGAAAAAGCATCATAGTGAAAAAATGTCAGAGTCCAAGGATGAAAATCCTGACCTTCCTCCCAAGTCAAAGCCACAGGAGGAACATGGTTTTGCCATGGATTTTGACATTAGCCTGGAAAGTCTATGGCCCTCGGATCATATATCTTTGGTCTCTAACCCCATGTCTCCTTTTCTCTTTTCCACTATCTCTGACCAACCTTGTTCTCCTGTCTGGGCTTTTTCTGATGCCGAAGATGAGAGGCTAATCAGGATTGCAGCTTCAGCAGGTAATACAAATACAACAACTGAAAATCTAGTTGAAAATTATGACAACAAAAAAACTGTGCCACCCCTTGTAGCCATCCCAACTTCAGAAAATCCAGATGCTTATTGTCTAATCAAGGAAAGAATGACACAAGCGCTTCGCCACTTCAAAGAGTTGACTGAACAAAATGTTCTAGCTCAGGTTTGGGCACCTATGAGGAATGGAAATCGGTATGCACTTACAACTTCAGGCCAACCATTTGTTCTTGATCCACATAGTAATGGACTCCATCAGTATAGAACAGTTTCCCTGATGTATATGTTCTCTGTGGATGGGGAGAATGATGAGATCATGGGACTTCCCGGCCGAGTTTTTCAGCAAAAAATTCCAGAATGGACTCCCAATGTTCAGTTTTATTCTAGTAAAGAGTATCAGCGCCTAAATCACGCGCAACATTACAATGTCCGCGGAACCTTGGCCCTGCCTGTGTTTGAGCTTGCAGGACAGTCATGTGTGGCTGTAGTGGAGTTGATAATGACTTCACAGAAGATTAACTATGCTCCTGAGGTTGATAAAATCTGCAAAGCCCTTGAGGCGGTTAAGTTGAGGAGTTCAGAAATTTTGGAGCATCAATACATTCAGATATGTAACGAAGACCGCCAGTATGCATTAGCCGAGATTTTGGAGATACTGACAGTTGTGTGTGAAACTCATAGTTTGCCTCTGGCACAAACATGGGTTCCATGTAAGCATCGGAGTGTCTTAGCACATGGCGGTGGTCATAAGAAAAGTTGCTCAAGTTTTGATGGATGTTGCATGGGGCAAGTTTGCATGTCTATAACTGAGGTAGCATTCTATGTAATAGATGCTCATACATGGGGTTTTCATGAGGCCTGTGTTGAGCATCACTTACAACAAGGTCAAGGAGTTGCTGGGAGGGCATTTTTGTCCCACAACATGTGCTTCTGTGGAAACATCGCCCAATTCTGCAAAACTGAATATCCCTTAGTACATTATGCACTCATGTTTGGTTTAACCAGCTGTTTTGCAGTATGCTTACAAAGCTCTCATACCGGAAATGATGATTATGTGCTAGAGTTTTTTCTGCCTCCTGGGATCACAGACTTTAATGAACAAAAAAGATTGTTGGGATCCATATTGGCAACAATGAAGCCACATTTTCAGAGTCTTAAGATTGCTTCTGGCATTGAACTTGAGGAAAATGCTTCAATTGAAATCATAGAAGCAAGAAATGAAAGAGTTAATTTGAGGTTTGAGTCCATTCCAATTACTCAATCTTCTAAGTCACCACCTAGACATGCCTCGCCAAATGTGGGGGAGGGGCTGCCACTGGAGCCATCAGAACAGAAAATAATGGCATACTTTGATGGTATAAATGATGGAGGGAGTCTTGGTGATAATGCAGGTGGACACATTGATCAGAACACTTCCTTAAAGATCAAAACCAAAAAGAAACCCTCAGAGAGAAAACGTGGAAAAGCTGAGAAATCAATTAGTCTTGATGTTTTGCAACATTACTTCACAGGAAGCCTTAAGGATGCTGCAAAGAGCCTTGGTGTTTGCCCTACTACAATGAAGCGCATCTGTAGGCAGCATGGGATATCACGCTGGCCATCTCGAAAGATAAAAAAGGTTAACCGTTCCCTGTCCAAGCTCAAGTGTGTTATTGAATCAGTCCATGGTGCTGAACGAGCATTTGGTTTGAATTCTCTAAGTACCGTTTCACTTCCCATTGCTGCCGGTTCCTTTTCTGAGCCATCCACTTCAAACAAGTTCAACCGCCAAACATCATTGACCATTAGGCCATCGGAACCTAAGATCAATGAAAAGGATTTTGATGCGTCTGGAGCATCAGAAACAAAAAGGCAGGCTGCAATGGAAGATCAATTCCTTGGACTTGAAGCAAGGGCACAAAGTCCTGAGAAAGTGATTAATGATAGAGGAGTGGCCACTCAGGAGATTGGCACTAAAGGCACAAACAAGTTTAGAACTGGAAGTGGCTCAAGTGAAAGCAGTGGAAATCCTACTCCTCATGGTTCATGCCATGGTAGTCCCCCAAATGAAATCTCACCTCCTAAAGATATATTTGTAACAGGCCACAGTGAGAAATGTCTTGTGTTAAGAGGATCACTGGGGTCAACAACACTGCACTCAACTAGTACACCGAACTGCACAACTGCATACCCTATGCCTCACATTGTAGAAACGACAGAACCTCAAGAACTATTTGGAGGACAGCTACTTGAGCGTGCTGGTAGTTCTAAAGACTTGAGAAATCTATGCCCTTCAGCAGATGCTGTTTTGGAGGATCAGGTTCCAGAAGCTTACAAGATGAATCCTCAATGTTCTGACTTGCCTCAAATGCAACACATGGATAACCTTAATAACACTCTGACACCTTTTGCAGTTAGGAAAGAGGTGAAGAGTGTGACTATTAAGGCAACATATAAAGAAGATATCATAAGGTTTAAGGTCTCTATGGACTGTGGTATTGTGGAACTGAAAGAGGAAATTGCCAAAAGATTGAAACTAGAGGCGGGAACATTTGATATCAAGTATCTGGATGATGATCATGAATGGGTTTTGATAGCCTGTGATGCAGACCTGCAGGAGTGCATGGACATCTCAAGATCATCAGGCAGCAACGTAATCAGGCTTGTGGTGCATGATATACTGCCCATTCTCGGAAGCTCTTGTGAGAGCTCAGGAGATTGGAAAGGTTGTATATAGTTTGGCTTGTATTATTCAATTATTGAGATTCTTGTAGTTATAAGTAATAGCTTTTTTTTTGTGATAAAATAATAGCTTTTTATCAAAAAAAGAGAAGAAAAAAAGTTTCTTACATGCGCAAATTGCAAGACACATATTATTGGATTTAAAGTCCAAGTTTTATAAT
CDS Sequence
  • >Glyma.09G137000.1
    ATGTCAGAGTCCAAGGATGAAAATCCTGACCTTCCTCCCAAGTCAAAGCCACAGGAGGAACATGGTTTTGCCATGGATTTTGACATTAGCCTGGAAAGTCTATGGCCCTCGGATCATATATCTTTGGTCTCTAACCCCATGTCTCCTTTTCTCTTTTCCACTATCTCTGACCAACCTTGTTCTCCTGTCTGGGCTTTTTCTGATGCCGAAGATGAGAGGCTAATCAGGATTGCAGCTTCAGCAGGTAATACAAATACAACAACTGAAAATCTAGTTGAAAATTATGACAACAAAAAAACTGTGCCACCCCTTGTAGCCATCCCAACTTCAGAAAATCCAGATGCTTATTGTCTAATCAAGGAAAGAATGACACAAGCGCTTCGCCACTTCAAAGAGTTGACTGAACAAAATGTTCTAGCTCAGGTTTGGGCACCTATGAGGAATGGAAATCGGTATGCACTTACAACTTCAGGCCAACCATTTGTTCTTGATCCACATAGTAATGGACTCCATCAGTATAGAACAGTTTCCCTGATGTATATGTTCTCTGTGGATGGGGAGAATGATGAGATCATGGGACTTCCCGGCCGAGTTTTTCAGCAAAAAATTCCAGAATGGACTCCCAATGTTCAGTTTTATTCTAGTAAAGAGTATCAGCGCCTAAATCACGCGCAACATTACAATGTCCGCGGAACCTTGGCCCTGCCTGTGTTTGAGCTTGCAGGACAGTCATGTGTGGCTGTAGTGGAGTTGATAATGACTTCACAGAAGATTAACTATGCTCCTGAGGTTGATAAAATCTGCAAAGCCCTTGAGGCGGTTAAGTTGAGGAGTTCAGAAATTTTGGAGCATCAATACATTCAGATATGTAACGAAGACCGCCAGTATGCATTAGCCGAGATTTTGGAGATACTGACAGTTGTGTGTGAAACTCATAGTTTGCCTCTGGCACAAACATGGGTTCCATGTAAGCATCGGAGTGTCTTAGCACATGGCGGTGGTCATAAGAAAAGTTGCTCAAGTTTTGATGGATGTTGCATGGGGCAAGTTTGCATGTCTATAACTGAGGTAGCATTCTATGTAATAGATGCTCATACATGGGGTTTTCATGAGGCCTGTGTTGAGCATCACTTACAACAAGGTCAAGGAGTTGCTGGGAGGGCATTTTTGTCCCACAACATGTGCTTCTGTGGAAACATCGCCCAATTCTGCAAAACTGAATATCCCTTAGTACATTATGCACTCATGTTTGGTTTAACCAGCTGTTTTGCAGTATGCTTACAAAGCTCTCATACCGGAAATGATGATTATGTGCTAGAGTTTTTTCTGCCTCCTGGGATCACAGACTTTAATGAACAAAAAAGATTGTTGGGATCCATATTGGCAACAATGAAGCCACATTTTCAGAGTCTTAAGATTGCTTCTGGCATTGAACTTGAGGAAAATGCTTCAATTGAAATCATAGAAGCAAGAAATGAAAGAGTTAATTTGAGGTTTGAGTCCATTCCAATTACTCAATCTTCTAAGTCACCACCTAGACATGCCTCGCCAAATGTGGGGGAGGGGCTGCCACTGGAGCCATCAGAACAGAAAATAATGGCATACTTTGATGGTATAAATGATGGAGGGAGTCTTGGTGATAATGCAGGTGGACACATTGATCAGAACACTTCCTTAAAGATCAAAACCAAAAAGAAACCCTCAGAGAGAAAACGTGGAAAAGCTGAGAAATCAATTAGTCTTGATGTTTTGCAACATTACTTCACAGGAAGCCTTAAGGATGCTGCAAAGAGCCTTGGTGTTTGCCCTACTACAATGAAGCGCATCTGTAGGCAGCATGGGATATCACGCTGGCCATCTCGAAAGATAAAAAAGGTTAACCGTTCCCTGTCCAAGCTCAAGTGTGTTATTGAATCAGTCCATGGTGCTGAACGAGCATTTGGTTTGAATTCTCTAAGTACCGTTTCACTTCCCATTGCTGCCGGTTCCTTTTCTGAGCCATCCACTTCAAACAAGTTCAACCGCCAAACATCATTGACCATTAGGCCATCGGAACCTAAGATCAATGAAAAGGATTTTGATGCGTCTGGAGCATCAGAAACAAAAAGGCAGGCTGCAATGGAAGATCAATTCCTTGGACTTGAAGCAAGGGCACAAAGTCCTGAGAAAGTGATTAATGATAGAGGAGTGGCCACTCAGGAGATTGGCACTAAAGGCACAAACAAGTTTAGAACTGGAAGTGGCTCAAGTGAAAGCAGTGGAAATCCTACTCCTCATGGTTCATGCCATGGTAGTCCCCCAAATGAAATCTCACCTCCTAAAGATATATTTGTAACAGGCCACAGTGAGAAATGTCTTGTGTTAAGAGGATCACTGGGGTCAACAACACTGCACTCAACTAGTACACCGAACTGCACAACTGCATACCCTATGCCTCACATTGTAGAAACGACAGAACCTCAAGAACTATTTGGAGGACAGCTACTTGAGCGTGCTGGTAGTTCTAAAGACTTGAGAAATCTATGCCCTTCAGCAGATGCTGTTTTGGAGGATCAGGTTCCAGAAGCTTACAAGATGAATCCTCAATGTTCTGACTTGCCTCAAATGCAACACATGGATAACCTTAATAACACTCTGACACCTTTTGCAGTTAGGAAAGAGGTGAAGAGTGTGACTATTAAGGCAACATATAAAGAAGATATCATAAGGTTTAAGGTCTCTATGGACTGTGGTATTGTGGAACTGAAAGAGGAAATTGCCAAAAGATTGAAACTAGAGGCGGGAACATTTGATATCAAGTATCTGGATGATGATCATGAATGGGTTTTGATAGCCTGTGATGCAGACCTGCAGGAGTGCATGGACATCTCAAGATCATCAGGCAGCAACGTAATCAGGCTTGTGGTGCATGATATACTGCCCATTCTCGGAAGCTCTTGTGAGAGCTCAGGAGATTGGAAAGGTTGTATATAG
Protein Sequence
  • >Glyma.09G137000.1
    MSESKDENPDLPPKSKPQEEHGFAMDFDISLESLWPSDHISLVSNPMSPFLFSTISDQPCSPVWAFSDAEDERLIRIAASAGNTNTTTENLVENYDNKKTVPPLVAIPTSENPDAYCLIKERMTQALRHFKELTEQNVLAQVWAPMRNGNRYALTTSGQPFVLDPHSNGLHQYRTVSLMYMFSVDGENDEIMGLPGRVFQQKIPEWTPNVQFYSSKEYQRLNHAQHYNVRGTLALPVFELAGQSCVAVVELIMTSQKINYAPEVDKICKALEAVKLRSSEILEHQYIQICNEDRQYALAEILEILTVVCETHSLPLAQTWVPCKHRSVLAHGGGHKKSCSSFDGCCMGQVCMSITEVAFYVIDAHTWGFHEACVEHHLQQGQGVAGRAFLSHNMCFCGNIAQFCKTEYPLVHYALMFGLTSCFAVCLQSSHTGNDDYVLEFFLPPGITDFNEQKRLLGSILATMKPHFQSLKIASGIELEENASIEIIEARNERVNLRFESIPITQSSKSPPRHASPNVGEGLPLEPSEQKIMAYFDGINDGGSLGDNAGGHIDQNTSLKIKTKKKPSERKRGKAEKSISLDVLQHYFTGSLKDAAKSLGVCPTTMKRICRQHGISRWPSRKIKKVNRSLSKLKCVIESVHGAERAFGLNSLSTVSLPIAAGSFSEPSTSNKFNRQTSLTIRPSEPKINEKDFDASGASETKRQAAMEDQFLGLEARAQSPEKVINDRGVATQEIGTKGTNKFRTGSGSSESSGNPTPHGSCHGSPPNEISPPKDIFVTGHSEKCLVLRGSLGSTTLHSTSTPNCTTAYPMPHIVETTEPQELFGGQLLERAGSSKDLRNLCPSADAVLEDQVPEAYKMNPQCSDLPQMQHMDNLNNTLTPFAVRKEVKSVTIKATYKEDIIRFKVSMDCGIVELKEEIAKRLKLEAGTFDIKYLDDDHEWVLIACDADLQECMDISRSSGSNVIRLVVHDILPILGSSCESSGDWKGCI*