Gene Details:

Functional Descriptions:

  • Overexpression of miR169c inhibited nodulation through targeting 3′-UTR of GmNFYA-C, whereas knockout miR169c through CRISPR-cas9 promoted nodulation.
  • Overexpression of GmNFYA-C promoted soybean nodulation through facilitating rhizobium infection and increasing the expression of symbiotic signaling gene GmENOD40.
  • GmNFYA-C directly induced the expression of GmENOD40. In addition, overexpression of GmNFYA-C without the target site of miR169c partially attenuated the inhibitory effect of high N on soybean nodulation.
  • We discovered a new regulatory pathway involving the miR169c-NFYA-C-ENOD40 module that regulates soybean nodulation in response to N availability.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions: K7LI34

Sequences:

cDNA Sequence
  • >Glyma.10G082800.5
    AACACATGTAAGAGTTCTCTTTGCCCCCTCTCTCACACTTTGCTTATTCTTCTCTCTTGTACGTGTTAGTGTGTACTATGTTTGTGTGTTTGTGTTTTGCTTCATTTAGTACCGACATGGCTCCAAACTAATGGGGGTGTTCCTCCATTCATCTACTTCTTCTTAGAAACCGATTCTGCCATACTCGTTCTTCTGCTTTTACCACTTGGGAGCATTCAAGTTTTGCTTGAGTCCTTATAGCTTAGGCTCTTTGGAACAATCTTAATCTTCTTTTTATAGAATTTTATTGGTAAAATACAAATTAAAGTTTTGTTTTTCATATCTATTTTTTTTTTATGACAGGATAAAATTTGAGGCAGAACTGTAGTTTCTAGAAACTTGACAGAGCTAAGAGAATCTTGGTAATTATAGGCAGTAGATTACTTTATTAACTGTGTATAAACAAGTCATCGTTTGGAGGCTATAGATGGCGATGCAAACCGTTTACCTGAAAGAACATGAAGGACTTGTCTACAATTCTGTTGGACAGTTGTCATCTGTAACTTCAGCTCCATGGTGGAGTGCCTTAGGATCTCAACCAGTTTATGGGGAGTATTGTGGTCAAATCAAACCCTTTTCGTTCGAGATTTCCAACTACGTCGACCAATTCCCAGCCGGTAAGCAAGCGGTACGAGGAGTTGAACAATTGTTGGATAAAGGACATACAACAACCCAGTTTACCATCTTTCCAGATGATTGTAAAATGTCAGGTGATGCAGAAAATCCTCAGGCAACCTTATCACTGCAGTCATCACTTGCTGAGCCCCACAATCGTTTTGAGATAGGGTTTAATCAGCCAATGATCTGTGCAAAATATCCTTACATGGATCAATTTTATGGACTCTTCTCAGCTTTTGGACCTCAAATTTCGGGACGGATAATGCTTCCAATTAACTTGACATCCGATGACGGACCAACTTACGTAAATGCGAAGCAGTACCATGGAATCATCAGACGTCGGCTATCACGTGCAAAAGCCGTACTTGAGAACAAAATGATTAAACGTCGCAAGCCATATATGCATGAATCACGCCATCTACATGCATTGCGGCGACCAAGAGGATGTGGTGGTCGCTTCTTGAACACAAAAGGCTCTACTAATGGAAATGGTAGAAATGAAAGCAAAGTGAATAAAACTGGCGGCCGACAATTGCAGTCCAGTGCCTCTCAGAGTTCTGAAGTCCTGCATTCTGAGGTTGGAACTTTAAATTCCTCAAAGGAGACAAATCGAAGCAGTCCAAACATCTCTGGTTCAGAGGTGACTAGCATGTATTCAAGGGAAGGTTTTGATGGTTTTTCTGTCAATCGTCTTGGATCTTCCGTTCACTCCCTAGTGGACATGGTATTATCACGCCCACTAAATGGGTTGCAGCAGTATCAGGTAACTGCTGCAACCTTAAAGTTTGATTAGCAACGAGACTAGTGGGCTTGGTGCTGCGATGTTGCAGCACCACCCTATCCTTGCAAGGGACCAGGTGAGGAACTTCTGTTGCAGTTTCTTGTGTTCATTGTGACGAATTTCGTTCTGGAGGTGTGATTCTTCATCTGGTTTTAGGCAAACCATTCTTGGCTACTGCTTAGGCAACTCATCCTTGGCTCATTATATTAGTAGTACTGTCTTTTTCTTTCTGCACAGGCTTGTCATGACATGGTCATACATGCTAATGGCTTCATATCCTGATGCAGTAGACTATGATGTGTCTGTGTTAAAATAACAAGAATTCTGGTTTCGTGTGTTTTTTAGTTATGTCTAGGTGTGGTATTTTGTTCTGGATTTCTGACTTTGTGTGGAGCTATGTAACATAGAACTCTGTATTGTGATCTTTGTTAGAAAAATAAAACATCGATCTGGACTTACCCTCC
  • >Glyma.10G082800.8
    AACACATGTAAGAGTTCTCTTTGCCCCCTCTCTCACACTTTGCTTATTCTTCTCTCTTGTACGTGTTAGTGTGTACTATGTTTGTGTGTTTGTGTTTTGCTTCATTTAGTACCGACATGGCTCCAAACTAATGGGGGTGTTCCTCCATTCATCTACTTCTTCTTAGAAACCGATTCTGCCATACTCGTTCTTCTGCTTTTACCACTTGGGAGCATTCAAGTTTTGCTTGAGTCCTTATAGCTTAGGCTCTTTGGAACAATCTTAATCTTCTTTTTATAGAATTTTATTGGTAAAATACAAATTAAAGTTTTGTTTTTCATATCTATTTTTTTTTTATGACAGGATAAAATTTGAGGCAGAACTGTAGTTTCTAGAAACTTGACAGAGCTAAGAGAATCTTGGTAATTATAGGCAGTAGATTACTTTATTAACTGTGTATAAACAAGTCATCGTTTGGAGGCTATAGATGGCGATGCAAACCGTTTACCTGAAAGAACATGAAGGACTTGTCTACAATTCTGTTGGACAGTTGTCATCTGTAACTTCAGCTCCATGGTGGAGTGCCTTAGGATCTCAACCAGTTTATGGGGAGTATTGTGGTCAAATCAAACCCTTTTCGTTCGAGATTTCCAACTACGTCGACCAATTCCCAGCCGGTAAGCAAGCGGTACGAGGAGTTGAACAATTGTTGGATAAAGGACATACAACAACCCAGTTTACCATCTTTCCAGATGATTGTAAAATGTCAGGTGATGCAGAAAATCCTCAGGCAACCTTATCACTGCAGTCATCACTTGCTGAGCCCCACAATCGTTTTGAGATAGGGTTTAATCAGCCAATGATCTGTGCAAAATATCCTTACATGGATCAATTTTATGGACTCTTCTCAGCTTTTGGACCTCAAATTTCGGGACGGATAATGCTTCCAATTAACTTGACATCCGATGACGGACCAACTTACGTAAATGCGAAGCAGTACCATGGAATCATCAGACGTCGGCTATCACGTGCAAAAGCCGTACTTGAGAACAAAATGATTAAACGTCGCAAGCCATATATGCATGAATCACGCCATCTACATGCATTGCGGCGACCAAGAGGATGTGGTGGTCGCTTCTTGAACACAAAAGGCTCTACTAATGGAAATGGTAGAAATGAAAGCAAAGTGAATAAAACTGGCGGCCGACAATTGCAGTCCAGTGCCTCTCAGAGTTCTGAAGTCCTGCATTCTGAGGTTGGAACTTTAAATTCCTCAAAGGAGACAAATCGAAGCAGTCCAAACATCTCTGGTTCAGAGGTGACTAGCATGTATTCAAGGGAAGGTTTTGATGGTTTTTCTGTCAATCGTCTTGGATCTTCCGTTCACTCCCTAGTGGACATGGTATTATCACGCCCACTAAATGGGTTGCAGCAGTATCAGGTAACTGCTGCAACCTTAAAGTTTGATTAGCAACGAGACTAGTGGGCTTGGTGCTGCGATGTTGCAGCACCACCCTATCCTTGCAAGGGACCAGGTGAGGAACTTCTGTTGCAGTTTCTTGTGTTCATTGTGACGAATTTCGTTCTGGAGGTGTGATTCTTCATCTGGTTTTAGGCAAACCATTCTTGGCTACTGCTTAGGCAACTCATCCTTGGCTCATTATATTAGTAGTACTGTCTTTTTCTTTCTGCACAGGCTTGTCATGACATGGTCATACATGCTAATGGCTTCATATCCTGATGCAGTAGACTATGATGTGTCTGTGTTAAAATAACAAGAATTCTGGTTTCGTGTGTTTTTTAGTTATGTCTAGGTGTGGTATTTTGTTCTGGATTTCTGACTTTGTGTGGAGCTATGTAACATAGAACTCTGTATTGTGATCTTTGTTAGAAAAATAAAACATCGATCTGGACTTACCCTCC
  • >Glyma.10G082800.7
    AACACATGTAAGAGTTCTCTTTGCCCCCTCTCTCACACTTTGCTTATTCTTCTCTCTTGTACGTGTTAGTGTGTACTATGTTTGTGTGTTTGTGTTTTGCTTCATTTAGTACCGACATGGCTCCAAACTAATGGGGGTGTTCCTCCATTCATCTACTTCTTCTTAGAAACCGATTCTGCCATACTCGTTCTTCTGCTTTTACCACTTGGGAGCATTCAAGTTTTGCTTGAGTCCTTATAGCTTAGGCTCTTTGGAACAATCTTAATCTTCTTTTTATAGAATTTTATTGGTAAAATACAAATTAAAGTTTTGTTTTTCATATCTATTTTTTTTTTATGACAGGATAAAATTTGAGGCAGAACTGTAGTTTCTAGAAACTTGACAGAGCTAAGAGAATCTTGGTAATTATAGGCAGTAGATTACTTTATTAACTGTGTATAAACAAGTCATCGTTTGGAGGCTATAGATGGCGATGCAAACCGTTTACCTGAAAGAACATGAAGGACTTGTCTACAATTCTGTTGGACAGTTGTCATCTGTAACTTCAGCTCCATGGTGGAGTGCCTTAGGATCTCAACCAGTTTATGGGGAGTATTGTGGTCAAATCAAACCCTTTTCGTTCGAGATTTCCAACTACGTCGACCAATTCCCAGCCGGTAAGCAAGCGGTACGAGGAGTTGAACAATTGTTGGATAAAGGACATACAACAACCCAGTTTACCATCTTTCCAGATGATTGTAAAATGTCAGGTGATGCAGAAAATCCTCAGGCAACCTTATCACTGCAGTCATCACTTGCTGAGCCCCACAATCGTTTTGAGATAGGGTTTAATCAGCCAATGATCTGTGCAAAATATCCTTACATGGATCAATTTTATGGACTCTTCTCAGCTTTTGGACCTCAAATTTCGGGACGGATAATGCTTCCAATTAACTTGACATCCGATGACGGACCAACTTACGTAAATGCGAAGCAGTACCATGGAATCATCAGACGTCGGCTATCACGTGCAAAAGCCGTACTTGAGAACAAAATGATTAAACGTCGCAAGCCATATATGCATGAATCACGCCATCTACATGCATTGCGGCGACCAAGAGGATGTGGTGGTCGCTTCTTGAACACAAAAGGCTCTACTAATGGAAATGGTAGAAATGAAAGCAAAGTGAATAAAACTGGCGGCCGACAATTGCAGTCCAGTGCCTCTCAGAGTTCTGAAGTCCTGCATTCTGAGGTTGGAACTTTAAATTCCTCAAAGGAGACAAATCGAAGCAGTCCAAACATCTCTGGTTCAGAGGTGACTAGCATGTATTCAAGGGAAGGTTTTGATGGTTTTTCTGTCAATCGTCTTGGATCTTCCGTTCACTCCCTAGTGGACATGGTATTATCACGCCCACTAAATGGGTTGCAGCAGTATCAGGTAACTGCTGCAACCTTAAAGTTTGATTAGCAACGAGACTAGTGGGCTTGGTGCTGCGATGTTGCAGCACCACCCTATCCTTGCAAGGGACCAGGTGAGGAACTTCTGTTGCAGTTTCTTGTGTTCATTGTGACGAATTTCGTTCTGGAGGTGTGATTCTTCATCTGGTTTTAGGCAAACCATTCTTGGCTACTGCTTAGGCAACTCATCCTTGGCTCATTATATTAGTAGTACTGTCTTTTTCTTTCTGCACAGGCTTGTCATGACATGGTCATACATGCTAATGGCTTCATATCCTGATGCAGTAGACTATGATGTGTCTGTGTTAAAATAACAAGAATTCTGGTTTCGTGTGTTTTTTAGTTATGTCTAGGTGTGGTATTTTGTTCTGGATTTCTGACTTTGTGTGGAGCTATGTAACATAGAACTCTGTATTGTGATCTTTGTTAGAAAAATAAAACATCGATCTGGACTTACCCTCC
CDS Sequence
  • >Glyma.10G082800.5
    ATGGCGATGCAAACCGTTTACCTGAAAGAACATGAAGGACTTGTCTACAATTCTGTTGGACAGTTGTCATCTGTAACTTCAGCTCCATGGTGGAGTGCCTTAGGATCTCAACCAGTTTATGGGGAGTATTGTGGTCAAATCAAACCCTTTTCGTTCGAGATTTCCAACTACGTCGACCAATTCCCAGCCGGTAAGCAAGCGGTACGAGGAGTTGAACAATTGTTGGATAAAGGACATACAACAACCCAGTTTACCATCTTTCCAGATGATTGTAAAATGTCAGGTGATGCAGAAAATCCTCAGGCAACCTTATCACTGCAGTCATCACTTGCTGAGCCCCACAATCGTTTTGAGATAGGGTTTAATCAGCCAATGATCTGTGCAAAATATCCTTACATGGATCAATTTTATGGACTCTTCTCAGCTTTTGGACCTCAAATTTCGGGACGGATAATGCTTCCAATTAACTTGACATCCGATGACGGACCAACTTACGTAAATGCGAAGCAGTACCATGGAATCATCAGACGTCGGCTATCACGTGCAAAAGCCGTACTTGAGAACAAAATGATTAAACGTCGCAAGCCATATATGCATGAATCACGCCATCTACATGCATTGCGGCGACCAAGAGGATGTGGTGGTCGCTTCTTGAACACAAAAGGCTCTACTAATGGAAATGGTAGAAATGAAAGCAAAGTGAATAAAACTGGCGGCCGACAATTGCAGTCCAGTGCCTCTCAGAGTTCTGAAGTCCTGCATTCTGAGGTTGGAACTTTAAATTCCTCAAAGGAGACAAATCGAAGCAGTCCAAACATCTCTGGTTCAGAGGTGACTAGCATGTATTCAAGGGAAGGTTTTGATGGTTTTTCTGTCAATCGTCTTGGATCTTCCGTTCACTCCCTAGTGGACATGGTATTATCACGCCCACTAAATGGGTTGCAGCAGTATCAGGTAACTGCTGCAACCTTAAAGTTTGATTAG
  • >Glyma.10G082800.8
    ATGGCGATGCAAACCGTTTACCTGAAAGAACATGAAGGACTTGTCTACAATTCTGTTGGACAGTTGTCATCTGTAACTTCAGCTCCATGGTGGAGTGCCTTAGGATCTCAACCAGTTTATGGGGAGTATTGTGGTCAAATCAAACCCTTTTCGTTCGAGATTTCCAACTACGTCGACCAATTCCCAGCCGGTAAGCAAGCGGTACGAGGAGTTGAACAATTGTTGGATAAAGGACATACAACAACCCAGTTTACCATCTTTCCAGATGATTGTAAAATGTCAGGTGATGCAGAAAATCCTCAGGCAACCTTATCACTGCAGTCATCACTTGCTGAGCCCCACAATCGTTTTGAGATAGGGTTTAATCAGCCAATGATCTGTGCAAAATATCCTTACATGGATCAATTTTATGGACTCTTCTCAGCTTTTGGACCTCAAATTTCGGGACGGATAATGCTTCCAATTAACTTGACATCCGATGACGGACCAACTTACGTAAATGCGAAGCAGTACCATGGAATCATCAGACGTCGGCTATCACGTGCAAAAGCCGTACTTGAGAACAAAATGATTAAACGTCGCAAGCCATATATGCATGAATCACGCCATCTACATGCATTGCGGCGACCAAGAGGATGTGGTGGTCGCTTCTTGAACACAAAAGGCTCTACTAATGGAAATGGTAGAAATGAAAGCAAAGTGAATAAAACTGGCGGCCGACAATTGCAGTCCAGTGCCTCTCAGAGTTCTGAAGTCCTGCATTCTGAGGTTGGAACTTTAAATTCCTCAAAGGAGACAAATCGAAGCAGTCCAAACATCTCTGGTTCAGAGGTGACTAGCATGTATTCAAGGGAAGGTTTTGATGGTTTTTCTGTCAATCGTCTTGGATCTTCCGTTCACTCCCTAGTGGACATGGTATTATCACGCCCACTAAATGGGTTGCAGCAGTATCAGGTAACTGCTGCAACCTTAAAGTTTGATTAG
  • >Glyma.10G082800.7
    ATGGCGATGCAAACCGTTTACCTGAAAGAACATGAAGGACTTGTCTACAATTCTGTTGGACAGTTGTCATCTGTAACTTCAGCTCCATGGTGGAGTGCCTTAGGATCTCAACCAGTTTATGGGGAGTATTGTGGTCAAATCAAACCCTTTTCGTTCGAGATTTCCAACTACGTCGACCAATTCCCAGCCGGTAAGCAAGCGGTACGAGGAGTTGAACAATTGTTGGATAAAGGACATACAACAACCCAGTTTACCATCTTTCCAGATGATTGTAAAATGTCAGGTGATGCAGAAAATCCTCAGGCAACCTTATCACTGCAGTCATCACTTGCTGAGCCCCACAATCGTTTTGAGATAGGGTTTAATCAGCCAATGATCTGTGCAAAATATCCTTACATGGATCAATTTTATGGACTCTTCTCAGCTTTTGGACCTCAAATTTCGGGACGGATAATGCTTCCAATTAACTTGACATCCGATGACGGACCAACTTACGTAAATGCGAAGCAGTACCATGGAATCATCAGACGTCGGCTATCACGTGCAAAAGCCGTACTTGAGAACAAAATGATTAAACGTCGCAAGCCATATATGCATGAATCACGCCATCTACATGCATTGCGGCGACCAAGAGGATGTGGTGGTCGCTTCTTGAACACAAAAGGCTCTACTAATGGAAATGGTAGAAATGAAAGCAAAGTGAATAAAACTGGCGGCCGACAATTGCAGTCCAGTGCCTCTCAGAGTTCTGAAGTCCTGCATTCTGAGGTTGGAACTTTAAATTCCTCAAAGGAGACAAATCGAAGCAGTCCAAACATCTCTGGTTCAGAGGTGACTAGCATGTATTCAAGGGAAGGTTTTGATGGTTTTTCTGTCAATCGTCTTGGATCTTCCGTTCACTCCCTAGTGGACATGGTATTATCACGCCCACTAAATGGGTTGCAGCAGTATCAGGTAACTGCTGCAACCTTAAAGTTTGATTAG
Protein Sequence
  • >Glyma.10G082800.5
    MAMQTVYLKEHEGLVYNSVGQLSSVTSAPWWSALGSQPVYGEYCGQIKPFSFEISNYVDQFPAGKQAVRGVEQLLDKGHTTTQFTIFPDDCKMSGDAENPQATLSLQSSLAEPHNRFEIGFNQPMICAKYPYMDQFYGLFSAFGPQISGRIMLPINLTSDDGPTYVNAKQYHGIIRRRLSRAKAVLENKMIKRRKPYMHESRHLHALRRPRGCGGRFLNTKGSTNGNGRNESKVNKTGGRQLQSSASQSSEVLHSEVGTLNSSKETNRSSPNISGSEVTSMYSREGFDGFSVNRLGSSVHSLVDMVLSRPLNGLQQYQVTAATLKFD*
  • >Glyma.10G082800.8
    MAMQTVYLKEHEGLVYNSVGQLSSVTSAPWWSALGSQPVYGEYCGQIKPFSFEISNYVDQFPAGKQAVRGVEQLLDKGHTTTQFTIFPDDCKMSGDAENPQATLSLQSSLAEPHNRFEIGFNQPMICAKYPYMDQFYGLFSAFGPQISGRIMLPINLTSDDGPTYVNAKQYHGIIRRRLSRAKAVLENKMIKRRKPYMHESRHLHALRRPRGCGGRFLNTKGSTNGNGRNESKVNKTGGRQLQSSASQSSEVLHSEVGTLNSSKETNRSSPNISGSEVTSMYSREGFDGFSVNRLGSSVHSLVDMVLSRPLNGLQQYQVTAATLKFD*
  • >Glyma.10G082800.7
    MAMQTVYLKEHEGLVYNSVGQLSSVTSAPWWSALGSQPVYGEYCGQIKPFSFEISNYVDQFPAGKQAVRGVEQLLDKGHTTTQFTIFPDDCKMSGDAENPQATLSLQSSLAEPHNRFEIGFNQPMICAKYPYMDQFYGLFSAFGPQISGRIMLPINLTSDDGPTYVNAKQYHGIIRRRLSRAKAVLENKMIKRRKPYMHESRHLHALRRPRGCGGRFLNTKGSTNGNGRNESKVNKTGGRQLQSSASQSSEVLHSEVGTLNSSKETNRSSPNISGSEVTSMYSREGFDGFSVNRLGSSVHSLVDMVLSRPLNGLQQYQVTAATLKFD*